Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,427,023 C→T 82.2% L80F (CTC→TTC)  ptsP → phosphoenolpyruvate‑‑protein phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,427,0230CT82.2% 839.7 / 161.8 353L80F (CTC→TTC) ptsPphosphoenolpyruvate‑‑protein phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (33/30);  new base T (102/188);  total (135/218)
Fisher's exact test for biased strand distribution p-value = 1.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

CTGATTCTGAAGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGAATATATGAAAGA  >  NZ_CP034943/1426886‑1427159
                                                                                                                                         |                                                                                                                                        
  gATTCTGAAGCGGAAGTACGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAg                                                                                                                                      <  1:5268737/140‑1 (MQ=255)
  gATTCTGAAGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAg                                                                                                                                      <  1:7728714/140‑1 (MQ=255)
   aTTCTGAAGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGt                                                                                                                                     <  2:7695693/140‑1 (MQ=255)
         aaGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCt                                                                                                                               >  1:58571/1‑140 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  2:3142908/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  2:2394701/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  2:122896/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  1:2475180/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  2:5894279/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  2:6329538/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  2:676346/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  1:5450024/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  1:56872/140‑1 (MQ=255)
          aGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTCCTGCTCACCTTTTAGTTCTCAGTGACCCTg                                                                                                                              <  1:7584552/140‑1 (MQ=255)
            cGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAg                                                                                                                            <  1:4871960/140‑1 (MQ=255)
            cGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAg                                                                                                                            <  1:1104521/140‑1 (MQ=255)
             ggAATTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGc                                                                                                                           <  1:6573120/140‑1 (MQ=255)
             ggAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGc                                                                                                                           <  1:7395372/140‑1 (MQ=255)
             ggAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGc                                                                                                                           <  1:6081995/140‑1 (MQ=255)
              gAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCt                                                                                                                          >  1:6968022/1‑140 (MQ=255)
              gAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCt                                                                                                                          >  1:6936949/1‑140 (MQ=255)
              gAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCt                                                                                                                          >  1:1252036/1‑140 (MQ=255)
                   aaGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACACTGAGCTTCTGa                                                                                                                     >  2:4884656/1‑140 (MQ=255)
                    aGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGaa                                                                                                                    <  2:5685537/140‑1 (MQ=255)
                      ccGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAATAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTATTTCTCAGTGACCCTGAGCTTCTGAAcc                                                                                                                  >  2:7743201/1‑140 (MQ=255)
                      ccGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTGCTGAAcc                                                                                                                  >  2:2859434/1‑140 (MQ=255)
                         ttttGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTg                                                                                                                              <  2:1095810/125‑1 (MQ=255)
                         ttttGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCtg                                                                                                               >  2:1599114/1‑140 (MQ=255)
                         ttttGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCtg                                                                                                               >  2:3751062/1‑140 (MQ=255)
                         ttttGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTGTAGTTTTCAGTGACCCTGAGCTTCTGAACCCtg                                                                                                               >  1:1396477/1‑140 (MQ=255)
                          tttGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTATGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCtgt                                                                                                              >  2:2691687/1‑140 (MQ=255)
                                 aaGCTATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTgaaagaa                                                                                                       <  1:5587003/140‑1 (MQ=255)
                                 aaGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTgaaagaa                                                                                                       <  1:170110/140‑1 (MQ=255)
                                 aaGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTgaaagaa                                                                                                       <  1:3658459/140‑1 (MQ=255)
                                 aaGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTgaaagaa                                                                                                       <  1:3244050/140‑1 (MQ=255)
                                   gCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGaaaa                                                                                                     >  2:7124866/1‑140 (MQ=255)
                                      aTTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAAt                                                                                                  >  2:1826564/1‑140 (MQ=255)
                                           accttCACAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCa                                                                                             <  2:2266401/137‑1 (MQ=255)
                                           aCGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCa                                                                                             <  1:1769458/140‑1 (MQ=255)
                                              ttCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTg                                                                                          >  1:2221438/1‑140 (MQ=255)
                                                cAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGAt                                                                                        <  1:3442575/140‑1 (MQ=255)
                                                        gagCTCGAAAAAATAAAAGAGCACGCTTTGAAAGAACTGGGTCTGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTAAGTGACCCTGAGATTGTGAAGAGGGTGAAAGAAAATATAAGGACTGATTAAGTGaa                                                                                >  2:970075/1‑140 (MQ=255)
                                                         agCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGact                                                                               <  1:1237553/140‑3 (MQ=255)
                                                         agCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAAt                                                                               >  1:5402210/1‑140 (MQ=255)
                                                         agCTAGAAATATTCAAAGAGCACTCTTTGTAAGATCTTTGTCAGGATAAATCTGTTATTTTTTATTCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTGTGAACCCTGTGAAAGAAAAAATCAGCACTGATTAATTGact                                                                               <  1:4414538/140‑3 (MQ=255)
                                                           cTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGc                                                                             >  2:5998318/1‑140 (MQ=255)
                                                           cTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCAATGATTCACTGAATGc                                                                             >  2:6781143/1‑140 (MQ=255)
                                                                 aaaaTCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAAtt                                                                       <  2:1773421/140‑1 (MQ=255)
                                                                 aaaaTCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAAtt                                                                       <  1:7271304/140‑1 (MQ=255)
                                                                      cAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCtt                                                                  <  2:7268495/140‑1 (MQ=255)
                                                                      aaaaGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCtt                                                                  <  2:5535090/139‑1 (MQ=255)
                                                                        aaGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTa                                                                <  1:6439874/140‑1 (MQ=255)
                                                                             cACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGa                                                           >  2:6832977/1‑140 (MQ=255)
                                                                              aCGCTTTGAACGAACTGGGTCAGGATATAGCTGATATTTTTTCGGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCACCACGGATTCAGTGAATGCTTAATTCGCTTTAAAAGaa                                                          <  2:5829332/140‑1 (MQ=255)
                                                                              aCGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCGGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGaa                                                          <  1:7746774/140‑1 (MQ=255)
                                                                              aCGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGaa                                                          <  1:3871604/140‑1 (MQ=255)
                                                                               cggtgtGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTATGCTCACCTTTTAGTTTTCAGTGACCCTGAGATTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTACAAGtaa                                                         >  2:5339893/6‑140 (MQ=255)
                                                                               cGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTACTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGaaa                                                         <  1:2859434/140‑1 (MQ=255)
                                                                                gCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAAc                                                        <  2:1015704/140‑1 (MQ=255)
                                                                                  tttGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGt                                                      <  2:6046858/140‑1 (MQ=255)
                                                                                           aCTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGt                                             <  1:4173126/140‑1 (MQ=255)
                                                                                           aCTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGt                                             <  1:7664640/140‑1 (MQ=255)
                                                                                            cTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGtt                                            <  2:7045700/140‑1 (MQ=255)
                                                                                            cTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGtt                                            <  2:4630446/140‑1 (MQ=255)
                                                                                              gggTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCg                                          <  2:3577813/140‑1 (MQ=255)
                                                                                              gggTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTGTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCg                                          <  2:1396477/140‑1 (MQ=255)
                                                                                               ggTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGt                                         <  1:6282515/140‑1 (MQ=255)
                                                                                                 tCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTa                                       <  2:3899264/140‑1 (MQ=255)
                                                                                                    ggATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAATCGTCTTCTATGTTCGTTACTa                                    >  1:1550363/1‑140 (MQ=255)
                                                                                                    ggATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTa                                    >  1:7043791/1‑140 (MQ=255)
                                                                                                         aaGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGttt                               >  1:3154277/1‑140 (MQ=255)
                                                                                                         aaGCTGATATTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAGGAAACGTCTTCTATGTTCGTTACTATGttt                               >  2:3242938/1‑140 (MQ=255)
                                                                                                          aGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACt                                     >  1:452508/1‑133 (MQ=255)
                                                                                                          aGCTGATATTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACt                                     <  2:5141912/133‑1 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  1:2380188/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  1:101317/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  2:7618832/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  2:6549282/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  1:2550672/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  1:873423/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  1:2729270/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  2:4120048/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTTTCAGGGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  1:2254135/1‑140 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGtt                                <  2:200715/133‑1 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGtt                                >  1:200715/1‑133 (MQ=255)
                                                                                                               atatTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCa                         >  1:6762477/1‑140 (MQ=255)
                                                                                                                          gCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGa              <  1:3089123/140‑1 (MQ=255)
                                                                                                                          gCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGa              <  1:6306562/140‑1 (MQ=255)
                                                                                                                          gCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGa              <  2:7386576/140‑1 (MQ=255)
                                                                                                                          gCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGa              <  1:5127323/140‑1 (MQ=255)
                                                                                                                          gCTCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTCAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGa              <  1:4696718/140‑1 (MQ=255)
                                                                                                                          gCTCAACTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGa              <  2:4461737/140‑1 (MQ=255)
                                                                                                                          gCTAACATTTTAGTTTTCAGTGACCCTTAGCTTCTGAACCCTGTGAAATAAAAAATCAGCAATGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGa              <  1:1947676/140‑1 (MQ=255)
                                                                                                                            tCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGAat            <  2:1483364/140‑1 (MQ=255)
                                                                                                                            tCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGAat            <  1:3751062/140‑1 (MQ=255)
                                                                                                                            tCACCTTTTAGTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGAat            <  1:4070681/140‑1 (MQ=255)
                                                                                                                            tCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGAat            <  1:707886/140‑1 (MQ=255)
                                                                                                                            acACCTTTTAGATTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGAACTGATTCAGTTAATGCTGAATTCGCTTTAAAACAAACGTATTATATGTTCGTTACTATGTTAGAATCAATGGACAACGaag            <  2:7582390/139‑2 (MQ=255)
                                                                                                                                      gTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGAATATATGAAaga  <  2:4876713/140‑1 (MQ=255)
                                                                                                                                      gTTTTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGAATATATGAAaga  <  2:3484163/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CTGATTCTGAAGCGGAAGTAAGCCGTTTTGATGAAGCGATTGCACGTTCAAAAGAAGAGCTCGAAAAAATCAAAGAGCACGCTTTGAAAGAACTGGGTCAGGATAAAGCTGATATTTTTTCTGCTCACCTTTTAGTTCTCAGTGACCCTGAGCTTCTGAACCCTGTGAAAGAAAAAATCAGCACTGATTCAGTGAATGCTGAATTCGCTTTAAAAGAAACGTCTTCTATGTTCGTTACTATGTTTGAATCAATGGACAACGAATATATGAAAGA  >  NZ_CP034943/1426886‑1427159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: