Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,485,790 G→T 5.8% D52Y (GAT→TAT)  ktrC → Ktr system potassium transporter KtrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,485,7900GT 5.8% 1036.7 / 11.6 365D52Y (GAT→TAT) ktrCKtr system potassium transporter KtrC
Reads supporting (aligned to +/- strand):  ref base G (188/141);  new base T (2/32);  total (190/175)
Fisher's exact test for biased strand distribution p-value = 1.92e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

GCGGTAATTGGACTTGGCCGTTTTGGTGGCAGTATTTGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCATTACAGT  >  NZ_CP034943/1485652‑1485929
                                                                                                                                          |                                                                                                                                           
gcgggcaTTGGCCTGGGCCGTTTTGGTGGCAGTCTTTGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGAGAAAGTCAACGACTATGCCAAGATCGCATCCCACGCCGTTATCGGTGa                                                                                                                                            <  2:922582/134‑1 (MQ=255)
    tAATTGGACTTGGCCGTTTTGGTGGCAGTATTTGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCa                                                                                                                                        <  2:5097237/140‑1 (MQ=255)
    gaaTTGGACTTGGCCGTTTTGGTTGCATTATTTTCAAAGCATTACTCGTAGAAGGCGTCGAGGTTATGGCGATGGAGATCTATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCa                                                                                                                                        <  2:1556531/139‑1 (MQ=255)
         ggACTTGGCCGTTTTGGTGGCAGTATTTGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGa                                                                                                                                   <  2:7767472/140‑1 (MQ=255)
              tGGCCGTTTTGGTGGCAGTATTTGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAAt                                                                                                                              <  2:1797728/140‑1 (MQ=255)
              tGGCCGTTTTGGTGGCAGTATTTGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGGTATCGGTGATTCAACAGACGAAt                                                                                                                              >  2:294164/1‑140 (MQ=255)
               ggCCGTTTTGGTGGCAGTATTTGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATc                                                                                                                             >  2:274985/1‑140 (MQ=255)
                                  tttGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGt                                                                                                          <  2:5492345/140‑1 (MQ=255)
                                        aaGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGa                                                                                                    >  1:435459/1‑140 (MQ=255)
                                        aaGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGa                                                                                                    >  2:6530547/1‑140 (MQ=255)
                                          gCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGGCAAGATCGCATCCGACGCCGTTATCGGTGATTCCACAGACGAATGTGTCCTGAAAAGGTTGGGGGTGCGaag                                                                                                  >  1:4264817/1‑139 (MQ=255)
                                          gCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGCTTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGaaa                                                                                                  >  2:4100614/1‑140 (MQ=255)
                                          gCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGaaa                                                                                                  >  1:2332046/1‑140 (MQ=255)
                                           cATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAAt                                                                                                 >  2:3542025/1‑140 (MQ=255)
                                              tAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAAtttt                                                                                              >  2:4740178/1‑140 (MQ=255)
                                                aGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGa                                                                                            <  2:364857/140‑1 (MQ=255)
                                                 gCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGAc                                                                                           <  1:5929636/140‑1 (MQ=255)
                                                 gCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGAc                                                                                           <  1:7448113/140‑1 (MQ=255)
                                                 gCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGAc                                                                                           <  1:4804391/140‑1 (MQ=255)
                                                 gCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGAc                                                                                           <  1:2996369/140‑1 (MQ=255)
                                                 gCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGAc                                                                                           <  1:2936742/140‑1 (MQ=255)
                                                 gCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGAc                                                                                           <  1:2813063/140‑1 (MQ=255)
                                                 gCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGAc                                                                                           <  2:694901/140‑1 (MQ=255)
                                                     agaagGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGACTATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACg                                                                                       >  1:1175980/1‑140 (MQ=255)
                                                     agaagGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACg                                                                                       >  1:1005597/1‑140 (MQ=255)
                                                     agaagGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACg                                                                                       >  2:1967785/1‑140 (MQ=255)
                                                     agaagGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACg                                                                                       >  1:3826464/1‑140 (MQ=255)
                                                     agaagGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACg                                                                                       >  2:445868/1‑140 (MQ=255)
                                                     agaagGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACg                                                                                       >  1:2106479/1‑140 (MQ=255)
                                                     agaagGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACg                                                                                       >  1:4026808/1‑140 (MQ=255)
                                                     agaagGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACg                                                                                       <  2:7371568/140‑1 (MQ=255)
                                                           cGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTAGTg                                                                                 >  2:6294416/1‑140 (MQ=255)
                                                                  gTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAAt                                                                          <  1:6178388/140‑1 (MQ=255)
                                                                     aTGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCgg                                                                       >  1:5436776/1‑140 (MQ=255)
                                                                     aTGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCgg                                                                       >  2:3220456/1‑140 (MQ=255)
                                                                      tGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAAt                                                                                                                              >  1:2544058/1‑84 (MQ=255)
                                                                          gATGTATATCGATGAAGATAAAGTCAACTAATATGACAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaa                                                                  <  2:3826464/140‑1 (MQ=255)
                                                                          gATGGATATCGATGACGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaa                                                                  <  2:5625511/140‑1 (MQ=255)
                                                                           aTGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaaa                                                                 >  2:6473046/1‑140 (MQ=255)
                                                                           aTGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaaa                                                                 >  2:5721286/1‑140 (MQ=255)
                                                                           aTGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaaa                                                                 >  2:6902903/1‑140 (MQ=255)
                                                                           aTGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaaa                                                                 >  2:264354/1‑140 (MQ=255)
                                                                           aTGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaaa                                                                 >  2:2151020/1‑140 (MQ=255)
                                                                           aTGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaaa                                                                 >  2:3502286/1‑140 (MQ=255)
                                                                           aTGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGaaaa                                                                 >  1:5247389/1‑140 (MQ=255)
                                                                            tGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAat                                                                >  1:6496336/1‑140 (MQ=255)
                                                                                 atCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCa                                                           <  2:3341511/140‑1 (MQ=255)
                                                                                    gATGAAGATAAAGTCACCGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGCTTCAACAGACGAATCTGTCCTGAAAAATTTGGGACTGCGAACTTGTGACCACGTTATTGTAGCAATCGGAGAAAATAGTCAAGc                                                        <  1:1936713/140‑1 (MQ=255)
                                                                                    gATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGc                                                        >  1:3431659/1‑140 (MQ=255)
                                                                                      tGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGa                                                      <  1:5761327/140‑1 (MQ=255)
                                                                                        aaGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAACATATTCAAGCGAGt                                                    >  2:4332488/1‑140 (MQ=255)
                                                                                               aGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTAAAGCGAGTATTTTaa                                             >  2:4994782/1‑140 (MQ=255)
                                                                                                  cAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGa                                          >  2:1453574/1‑140 (MQ=255)
                                                                                                  cAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGa                                          >  2:3091545/1‑140 (MQ=255)
                                                                                                    actaaTATGCAAAGATCGCATCCCACGCCGTTATCGGTTATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGAct                                        <  2:7201642/137‑1 (MQ=255)
                                                                                                    aCGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGAct                                        >  1:6592516/1‑140 (MQ=255)
                                                                                                     cGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGActc                                       >  2:1030649/1‑140 (MQ=255)
                                                                                                     cGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGActc                                       >  1:1528364/1‑140 (MQ=255)
                                                                                                     cGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGActc                                       >  1:6417764/1‑140 (MQ=255)
                                                                                                     cGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGActc                                       >  2:7544433/1‑140 (MQ=255)
                                                                                                      taaTATGCAAATATCGCATCCCACGCCGTTATCGTTTATTAAACACAACAATCTGTCATAAAAATTTTGGGTCTGGTAAATTTTGACCACGTTATTGTAGCAATGGTAGAAAATATTAAAGCGAGTATTTTAACGActct                                      <  2:4591932/139‑1 (MQ=255)
                                                                                                      gAATATGCCACCAGCGCATCCCACGCCGTTATCGGTGATGCAACACTCGAATCGTTGCTGAAAAATTTGGTCCTGCGAAATTTTGACCACGTTCTTTTCCCAACCGGAGAAATTATTCATGCGTGTATTTTAACGActct                                      <  2:5459296/140‑1 (MQ=255)
                                                                                                      gAATATGCCAAGATCGCATCCCACGCCTTTATCGGTGATTAAACAGACGAATCTTTCGTGAAAAATTTGGGTCTGCTAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCACGCGAGTATTTTAACGActct                                      <  2:136040/140‑1 (MQ=255)
                                                                                                      gAATATGCAAAGAGCGCATCCCACGCCGTGATCGGTGATTCCACAGACGAATCGGTCCTGAAAAATTTGGGTCTGCGAAATTTGGCCCACGTTTTTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGActct                                      <  2:6622549/140‑1 (MQ=255)
                                                                                                        atatGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCACTCGGAGAAACTATTCAAGCGAGTATTTTAACGACTCTCa                                    >  1:6342943/1‑140 (MQ=255)
                                                                                                         tttGCCAAGATCGTATCCCACGCCGTTATCGGTTATTAAACAGACGAACTTGTCCTTAAAATTTTGGGTGTGCTAAATTTTTACCACGTTTTTGTTGCATTCGGATAAAATATTAAAGCGAGTATTTTAACGACTCTCAt                                   <  2:7386573/138‑1 (MQ=255)
                                                                                                         tttGCAAATATCGCATCCCACGCCTTTATCGTTTATTCAACAGACTAATCTGTCCTTAAAAATTGGGGTCTGCTAAATTTTCACCACGTTATTGTACAAATCGGAGAAAATATTCACGCGAGTATTTTAACGACTCTCAt                                   <  1:3247038/138‑1 (MQ=255)
                                                                                                           tGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCa                                                                                         >  1:1382641/1‑84 (MQ=255)
                                                                                                               aaGATCGCATCCCACGCGGTTATGGGTGATTCAACAGACGAATCTGGCCTGAAAAATTTGGGTCTGCGAAATTTTGACCCCGTTATTGTAGCAATCGCAGAAAATATACACGCGAGTTTTTTAACGACTCTCATTCTGaa                             <  1:2641351/140‑1 (MQ=255)
                                                                                                                aGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGaat                            >  1:4567331/1‑139 (MQ=255)
                                                                                                                  aTCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGa                          >  1:3410561/1‑140 (MQ=255)
                                                                                                                    cGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAAc                        >  2:6193430/1‑140 (MQ=255)
                                                                                                                      cATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACtt                      >  2:533130/1‑140 (MQ=255)
                                                                                                                      cATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACtt                      >  1:6808149/1‑140 (MQ=255)
                                                                                                                      cATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACtt                      >  1:7268424/1‑140 (MQ=255)
                                                                                                                       aTCCCCCGCCTTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTg                     <  2:6314709/140‑1 (MQ=255)
                                                                                                                       aTCCCACGCCTTTATAGTTTATTAAACAGACCAATCTGTCCTGAAAAATTTGGTTCTGCTAATTTTTGACCACGTTATTGTACCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTg                     <  2:4894369/140‑1 (MQ=255)
                                                                                                                       aTCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTg                     >  1:1752948/1‑140 (MQ=255)
                                                                                                                       aTCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTg                     <  2:3410561/140‑1 (MQ=255)
                                                                                                                       aTCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTg                     <  1:7544433/140‑1 (MQ=255)
                                                                                                                        tCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTgg                    >  2:2202528/1‑140 (MQ=255)
                                                                                                                          caccGCCGTTAGCGTTTATACACCAGACGAATGTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCACGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCg                  <  2:4054622/137‑1 (MQ=255)
                                                                                                                            aCGCCGTTATCGGTGATTCAACAGACTAATCTGTCATTAAAAATTTGGGTCTGCTAAATTTTGACCACGTTATTGTACCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGtt                <  2:2016118/140‑1 (MQ=255)
                                                                                                                            aCGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGtt                <  2:4399791/140‑1 (MQ=255)
                                                                                                                              gcggTTATGGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCCCGTTTTTGTAGCAATCGGAGAAAATATTCACGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCa              <  1:1541021/137‑1 (MQ=255)
                                                                                                                              gCCGTTATGTGTTATTCAACAGACGAATCTGTCCTTAAAAATTTGGTTCTGCGAAATTTTGACCACGTTTTTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCa              <  2:4003694/140‑1 (MQ=255)
                                                                                                                              gCCGTTATCGTTTATACACCAGACGAATCTGTCCTGAAAAATTTGGGTCTGCTAAATTTTGACCACGTTATTGTAGAAATCGGACAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCa              <  1:819730/140‑1 (MQ=255)
                                                                                                                              gCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTTTTGTAGCAATCGGAGAAAATATTCAAGCGATTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCa              <  1:962192/140‑1 (MQ=255)
                                                                                                                              gCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCa              <  2:5247389/140‑1 (MQ=255)
                                                                                                                              gCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCa              <  1:4921078/140‑1 (MQ=255)
                                                                                                                                    aTCGGTTATTCAACAGACGAATCTGTCCTTAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGATAAAATATTCACGCGAGTTTTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAt        <  1:6972638/140‑1 (MQ=255)
                                                                                                                                    aTCGGTGATTCAACAGACTAATCTGTCATTAAAAATTTGGGTGTGCTATATTTTGACCAGTTTATTGTAGCAATCGGAGATAATATACAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAt        <  2:5790975/140‑1 (MQ=255)
                                                                                                                                    aTCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAt        >  1:5624152/1‑140 (MQ=255)
                                                                                                                                    aTCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAt        <  2:2427717/140‑1 (MQ=255)
                                                                                                                                    aTCGGTCATTCAACCCTCTAATGTGTCCTTAAAAATTTGCGTCTGCGAAATTTTGACCTCGTTATTGTAGCATTCGGTGAAAATTTTCTAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAt        <  2:5950552/140‑1 (MQ=255)
                                                                                                                                     tCGGTTATTCAAAAGAAGAATCTGTACTTAAAAATTTGGTTCTGAGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCTAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAtt       <  2:4273792/140‑1 (MQ=255)
                                                                                                                                     tCGGTTATACAACACACGAATCGGCCCTCAAAAATTGGGGTCGGCGAAATTTTGACCACTTTATTGTAGAAATCGGAGAAAATATTCACGCGTGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAtt       <  1:4548048/140‑1 (MQ=255)
                                                                                                                                     tCGGTGATTCAACAGACGAATCTGTCATTAAAATTTTGGTTCTGCCAAATTTTGACCCCGTTTTTGTAGCAATGGGAGAAAATATTCAAGCTAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAtt       <  1:3538618/140‑1 (MQ=255)
                                                                                                                                     tCGGTGATTAAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCTAAATTTTGACCACTTTATTGTAGAAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCAtt       <  2:1153101/140‑1 (MQ=255)
                                                                                                                                       ggTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCGGCGAAATTTTGACCACTTTATTGTAGCAATCGGATAAAATATTCAAGCGTGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCATTAc     <  1:2744231/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GCGGTAATTGGACTTGGCCGTTTTGGTGGCAGTATTTGCAAAGCATTAAGCGAAGAAGGCGTCGAGGTTATGGCGATGGATATCGATGAAGATAAAGTCAACGAATATGCCAAGATCGCATCCCACGCCGTTATCGGTGATTCAACAGACGAATCTGTCCTGAAAAATTTGGGTCTGCGAAATTTTGACCACGTTATTGTAGCAATCGGAGAAAATATTCAAGCGAGTATTTTAACGACTCTCATTCTGAAAGAACTTGGCGTTCATACCATTACAGT  >  NZ_CP034943/1485652‑1485929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: