Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,669,737 C→T 100% Q88* (CAG→TAG)  fliM → flagellar motor switch protein FliM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,669,7370CT100.0% 981.0 / ‑6.6 275Q88* (CAG→TAG) fliMflagellar motor switch protein FliM
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (147/126);  minor base G (1/0);  total (148/127)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 275 total aligned reads displayed.

TCAAACGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACCAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCGGGTA  >  NZ_CP034943/1669600‑1669871
                                                                                                                                         |                                                                                                                                      
tCAAACGTGCGCTGCGGTTGTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCAGATATCTGTCAGCTCTGTTGACTAg                                                                                                                                      >  2:250591/1‑140 (MQ=255)
 cAAACGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGACTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAgg                                                                                                                                     >  1:2608403/1‑140 (MQ=255)
  aaaCGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGt                                                                                                                                    <  1:6778905/140‑1 (MQ=255)
  aaaCGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGt                                                                                                                                    <  2:6783345/140‑1 (MQ=255)
     cGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTccc                                                                                                                                 <  2:4008484/140‑1 (MQ=255)
         cgcTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGt                                                                                                                                    <  2:7446431/133‑1 (MQ=255)
          gcTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATg                                                                                                                            <  1:4468579/140‑1 (MQ=255)
          gcTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATg                                                                                                                            <  1:80070/140‑1 (MQ=255)
           cTGCGGTTTTCCATGGATCAGATACGCAGTCTAACGATAATTCATGACAATTTTGCAAGACTTCTTACTAATCACTTTTCTGCTCAGCTCCGGACCTATATTCTCATATCTGTAAGCTCTGTTGAGTAGGTGCCTTATgt                                                                                                                           >  2:1651385/1‑139 (MQ=255)
               ggTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGaa                                                                                                                       <  1:4054156/140‑1 (MQ=255)
               ggTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGaa                                                                                                                       <  2:3444960/140‑1 (MQ=255)
               ggTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGaa                                                                                                                       <  1:6192525/140‑1 (MQ=255)
               ggTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGaa                                                                                                                       <  1:3083815/140‑1 (MQ=255)
                   ttCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATGTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTCGGTCCCTTATGAGGAATTTa                                                                                                                   >  2:3101880/1‑140 (MQ=255)
                    tCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTAt                                                                                                                  <  2:2856752/140‑1 (MQ=255)
                         ggATCATATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGat                                                                                                             <  2:3150400/140‑1 (MQ=255)
                         ggATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGat                                                                                                             <  2:424396/140‑1 (MQ=255)
                         ggATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGat                                                                                                             <  1:5100504/140‑1 (MQ=255)
                              aGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGatccatc                                                                                                        <  2:3695120/140‑1 (MQ=255)
                                     cAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACa                                                                                                 >  1:7082741/1‑140 (MQ=255)
                                          tAACGAGAATTCATGACAATTTTGCAAGAGTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACg                                                                                            >  1:2148609/1‑140 (MQ=255)
                                             cGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGAtt                                                                                         >  1:3589461/1‑140 (MQ=255)
                                             cGAGAATTCATGACAATTTTGCAAGACTTCTTACGACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGACTTTATCAGATCCATCCCGAACATGACGAtt                                                                                         >  1:5547968/1‑140 (MQ=255)
                                                  aatCATGACAATTTTGCAAGACTTCTGACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTAGGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                    >  1:1396402/3‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                    >  2:7620726/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                    >  1:6749416/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                    >  1:6623232/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                    >  1:6546070/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                    >  1:3197853/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                    >  1:4249747/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                    >  2:7061411/1‑140 (MQ=255)
                                                  aTTCATGAAAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATACACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCAATCCCGAACATGACGATTTTGaa                                                                                    >  1:4600545/1‑140 (MQ=255)
                                                   ttCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAAt                                                                                   <  1:6037782/140‑1 (MQ=255)
                                                    tCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTCTCAGCTCTGTTGACTAGGTCCCTTATGAGGAAATTATCAGATCCATCCCGAACATGACGATATTGAATc                                                                                  <  1:6958026/140‑1 (MQ=255)
                                                        gACAATTTTGCAAGACTTCTTACTAGTCACTGTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCGTTATGAGGAATTTATCAGATCCATCCCGAACATGACGTGTTTGAATCTGtt                                                                              >  1:1104385/1‑140 (MQ=255)
                                                        gACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGtt                                                                              >  1:6438364/1‑140 (MQ=255)
                                                           aaTTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGa                                                                           >  1:6345909/1‑140 (MQ=255)
                                                                   aaGCCTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATc                                                                   <  1:3754812/140‑1 (MQ=255)
                                                                      aCTTCTTACTACTCAGTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGa                                                                <  2:5615835/140‑1 (MQ=255)
                                                                        ttcttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATg                                                              <  1:5011546/140‑1 (MQ=255)
                                                                          cttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATAGa                                                            >  1:4532096/1‑140 (MQ=255)
                                                                             actactCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAgg                                                         <  1:162877/140‑1 (MQ=255)
                                                                             actactCACTTGTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAgg                                                         <  1:2884521/140‑1 (MQ=255)
                                                                               tactCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTa                                                       <  1:1730371/140‑1 (MQ=255)
                                                                                   cACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGAt                                                   <  2:1266022/140‑1 (MQ=255)
                                                                                   cACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGAt                                                   <  1:1440666/140‑1 (MQ=255)
                                                                                   cACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGAt                                                   <  2:4974975/140‑1 (MQ=255)
                                                                                    aCTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGAtt                                                  >  1:2693599/1‑140 (MQ=255)
                                                                                     cTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTa                                                 >  2:3198156/1‑140 (MQ=255)
                                                                                      ttttCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTat                                                >  2:1557961/1‑140 (MQ=255)
                                                                                      ttttCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCAATCCTATGGAAGGTAGGATTat                                                >  1:734040/1‑140 (MQ=255)
                                                                                         tCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTatgat                                             <  2:4939689/140‑1 (MQ=255)
                                                                                         tCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTatgat                                             <  1:3301922/140‑1 (MQ=255)
                                                                                         tCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTatgat                                             >  2:6184865/1‑140 (MQ=255)
                                                                                          cTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTatgatg                                            <  1:6040231/140‑1 (MQ=255)
                                                                                          atGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTatgatg                                            <  1:2325294/139‑1 (MQ=255)
                                                                                           tGCTCAGCTCAGGACCTATATTCACATATCTGTAAGCTCTGTTGACTAGGTCGCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATgg                                           >  2:6571691/1‑140 (MQ=255)
                                                                                             ctcagctcagGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAg                                         >  2:154957/1‑140 (MQ=255)
                                                                                                agatcagGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGtt                                      <  2:4901849/137‑1 (MQ=255)
                                                                                                  ctcagGACCTATATTCACATAGCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGACTTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTGCATCCGAGGGAAGGTAGGAGTCTGATGGAGGTTaa                                    >  2:1102687/1‑140 (MQ=255)
                                                                                                       gACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCGTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGCAGGTAGGATTCTGATGGCGGTTAACCCGa                               >  2:2146078/1‑140 (MQ=255)
                                                                                                       gACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGa                               >  2:5926449/1‑140 (MQ=255)
                                                                                                       gACCTATAGTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGAGTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGa                               <  2:5095383/140‑1 (MQ=255)
                                                                                                         ccTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACa                             <  1:5474076/140‑1 (MQ=255)
                                                                                                          cTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACaa                            <  2:3740068/140‑1 (MQ=255)
                                                                                                          cTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACaa                            >  1:3683317/1‑140 (MQ=255)
                                                                                                          cTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACaa                            <  2:6388650/140‑1 (MQ=255)
                                                                                                           tataTTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAt                           <  1:3462046/140‑1 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCGCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGa                                                                >  1:6247983/1‑102 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAgg                                                         <  2:782492/109‑1 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGTGTAGGATGGAGGTTAACCCGACAAtt                          >  2:3047004/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  1:1065959/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  1:207904/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  2:3904671/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  1:2551258/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  1:4072771/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  2:6009338/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  1:4508660/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  1:7545082/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTGATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACaaag                          >  2:5801999/1‑138 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCGTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                          >  1:880773/1‑140 (MQ=255)
                                                                                                               ttCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCa                       <  2:3550370/140‑1 (MQ=255)
                                                                                                               ttCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCa                       <  2:2208130/140‑1 (MQ=255)
                                                                                                                 cacaTATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCata                     <  2:5714750/140‑1 (MQ=255)
                                                                                                                 cacaTATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCata                     <  2:54532/140‑1 (MQ=255)
                                                                                                                    atatCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGTTGGAGGTTAACCCGACAATTGCATATAc                  >  1:1891495/1‑140 (MQ=255)
                                                                                                                    atatCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATAc                  >  1:4729598/1‑140 (MQ=255)
                                                                                                                    atatCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATAc                  >  1:1000246/1‑140 (MQ=255)
                                                                                                                       tCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGAGGGAGGTTAACCCGACAATTGCATATACgat               >  2:7715971/1‑140 (MQ=255)
                                                                                                                        cTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACgatg              <  1:2942267/140‑1 (MQ=255)
                                                                                                                           tCAGCTCTTTTGACTAGGTCACTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATc                                                                                  <  1:875545/69‑1 (MQ=255)
                                                                                                                            cAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATgg          >  2:1042305/1‑140 (MQ=255)
                                                                                                                            cAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAAAATGACGATTTTGAATCTGTTTGACGTCCATCCGATCGAAGGTAGGAGTATGATGGAGGTTAAACCGACAATTGCATATACGATGATgg          >  2:4523678/1‑140 (MQ=255)
                                                                                                                            cAGCTCTGTTGACTAGGTCCCTTAGGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATgg          >  2:7148964/1‑140 (MQ=255)
                                                                                                                              gCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGAt        >  2:2275027/1‑140 (MQ=255)
                                                                                                                              gCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACAATACAATTGCATATACGATGATGGAt        <  2:1726820/140‑1 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGa                                                            >  2:3882609/1‑84 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCata                     >  2:2077661/1‑123 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCggg    >  1:3543737/1‑140 (MQ=255)
                                                                                                                                    ttGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCGGGTa  <  2:6524936/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TCAAACGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACCAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCGGGTA  >  NZ_CP034943/1669600‑1669871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: