Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,820,732 C→A 7.4% S361Y (TCT→TAT)  EO946_RS09145 → thioesterase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,820,7320CA 7.4% 729.9 / 7.7 304S361Y (TCT→TAT) EO946_RS09145thioesterase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (130/135);  new base A (2/33);  total (132/172)
Fisher's exact test for biased strand distribution p-value = 2.45e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

GCGCTTGAGAGTGAGGGATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCGAG  >  NZ_CP034943/1820593‑1820871
                                                                                                                                           |                                                                                                                                           
gcgcTTGAGAGTGAGGGATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTc                                                                                                                                             >  1:3571041/1‑140 (MQ=255)
gcgcTTGAGAGTGAGGGATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTc                                                                                                                                             >  1:2328642/1‑140 (MQ=255)
    ttGAGAGTGAGGGATATCGCTATGTTTCTTTTATTGCTGAACACTCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCtata                                                                                                                                         <  1:2580607/140‑1 (MQ=255)
    ttGAGAGTGAGGGATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCtata                                                                                                                                         <  2:1877122/140‑1 (MQ=255)
            gAGGGATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAaatgaa                                                                                                                                 <  1:1190364/140‑1 (MQ=255)
            gAGGGATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAaatgaa                                                                                                                                 <  1:5776371/140‑1 (MQ=255)
            gAGGGAGATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAaatgaa                                                                                                                                 <  1:1841288/140‑1 (MQ=255)
             aGGGATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAaatgaat                                                                                                                                >  2:7099332/1‑140 (MQ=255)
              gggATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAaatgaatg                                                                                                                               <  1:6532227/140‑1 (MQ=255)
              gggATATCGATATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAaatgaatg                                                                                                                               <  1:4997725/140‑1 (MQ=255)
              gggAGATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAaatgaatg                                                                                                                               <  2:1182681/140‑1 (MQ=255)
                  tatCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGt                                                                                                                           >  2:3904798/1‑140 (MQ=255)
                   atCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTa                                                                                                                          >  2:3026902/1‑140 (MQ=255)
                     cGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTaaa                                                                                                                        >  1:3473596/1‑140 (MQ=255)
                       cTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTaaaat                                                                                                                      >  2:4369778/1‑139 (MQ=255)
                        tATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGCATGCTGTAAAAGc                                                                                                                     <  2:2699453/140‑1 (MQ=255)
                          tGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGcac                                                                                                                   <  2:4257602/140‑1 (MQ=255)
                               cTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTTCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTca                                                                                                              >  2:3673051/1‑140 (MQ=255)
                               cTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTca                                                                                                              >  2:6171358/1‑140 (MQ=255)
                               cTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATAAAAATGAATGCTGTAAAAGCACATTca                                                                                                              >  2:2472423/1‑140 (MQ=255)
                               attttATTGCTGAACAAACACATAAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGGCAAAACAAGAGAACTGAGACAGAAGAACATGCAGATTATATAAAAATGAAAGCTGTAAAAACACATAca                                                                                                              >  1:7439959/2‑140 (MQ=255)
                                 tttATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTcaca                                                                                                            <  2:640963/140‑1 (MQ=255)
                                    aTTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGa                                                                                                         <  2:4106020/140‑1 (MQ=255)
                                      tGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGaaa                                                                                                       >  1:4576953/1‑140 (MQ=255)
                                        cTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGACCTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGc                                                                                                     >  2:5795617/1‑140 (MQ=255)
                                        cTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGc                                                                                                     >  2:6699395/1‑140 (MQ=255)
                                          gAACAATCACCTCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGa                                                                                                   <  2:2801350/140‑1 (MQ=255)
                                          gAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGa                                                                                                   <  2:5220702/140‑1 (MQ=255)
                                          gAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGa                                                                                                   <  1:428977/140‑1 (MQ=255)
                                          gAACAATCACATCAGCTTGGCCAGCAGATCAGTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGa                                                                                                   >  1:7028140/1‑140 (MQ=255)
                                             cAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGCTGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGtc                                                                                                <  1:152467/140‑1 (MQ=255)
                                             cAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGtc                                                                                                <  1:5071541/140‑1 (MQ=255)
                                             cAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGtc                                                                                                <  1:3375814/140‑1 (MQ=255)
                                             cAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGAGTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGtc                                                                                                <  1:4509057/140‑1 (MQ=255)
                                              aaTCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGtct                                                                                               <  1:1273713/140‑1 (MQ=255)
                                                tCACATCAGCTTGGCCAGCAGATGATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGACGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCTCATTCACAGGACAGCGAGtctct                                                                                             >  2:7316604/1‑140 (MQ=255)
                                                tCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGtctct                                                                                             >  2:147347/1‑140 (MQ=255)
                                                tCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGtctct                                                                                             >  2:4801444/1‑140 (MQ=255)
                                                  acaTCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGACGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCa                                                                                           >  1:5868659/1‑140 (MQ=255)
                                                  acaTCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCa                                                                                           <  2:3473596/140‑1 (MQ=255)
                                                       aGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGaa                                                                                      >  2:7733859/1‑140 (MQ=255)
                                                            ggCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGcac                                                                                                                   >  2:5347827/1‑106 (MQ=255)
                                                              ccAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGa                                                                               >  2:4375730/1‑140 (MQ=255)
                                                               cagcagATCATTGTTGCTGAAAGGGAGGGAGTGGTCCGTCAAAATAAGAGAACTGAGAAATAAGAATATGCAGATTATATAAAAATGATGGCTGTAAAAGCACATTCACAGGAAAGCGATTCTCTCATTGAACAAACGAc                                                                              <  2:2913713/140‑1 (MQ=255)
                                                                  cagATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCa                                                                           >  2:587071/1‑140 (MQ=255)
                                                                  cagATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCa                                                                           >  2:2095292/1‑140 (MQ=255)
                                                                  cagATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCa                                                                           >  2:1802734/1‑140 (MQ=255)
                                                                    gATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAAt                                                                         <  2:3571041/140‑1 (MQ=255)
                                                                         ttgttgCTGAAATTGACGGAGTCGTCCTTAAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTATATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTtgtg                                                                    <  1:1599433/140‑1 (MQ=255)
                                                                               cTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGACCTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCAt                                                              >  2:1077611/1‑140 (MQ=255)
                                                                               cTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCAt                                                              >  2:5508231/1‑140 (MQ=255)
                                                                                    aGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCt                                                         >  1:2015717/1‑140 (MQ=255)
                                                                                    aGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCt                                                         >  1:6916472/1‑140 (MQ=255)
                                                                                      tGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTgg                                                       <  1:7733859/140‑1 (MQ=255)
                                                                                               cgtccttcAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGa                                              <  2:472297/140‑1 (MQ=255)
                                                                                                gtccgtcAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTGTATACAACTGAATGCTGTAAAAGCACATTCACAGGAACCCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGAt                                             <  2:1593503/140‑1 (MQ=255)
                                                                                                gtccgtcAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGtc                                                                                                <  2:1058700/89‑1 (MQ=255)
                                                                                                 tccttcAAAATAAGAGAACTGAGACAGAAGAAGATGCGGATTCTATACAACTGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCTTTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATc                                            <  2:256731/140‑1 (MQ=255)
                                                                                                 tccgtaAAAAGAAGATAACTGATAAAGAAGAATATGAAGATTATATAAAAATGAATGATTTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTTAAAAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATc                                            <  2:3265923/140‑1 (MQ=255)
                                                                                                   cgtcAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCaa                                          <  1:6641274/140‑1 (MQ=255)
                                                                                                     aaaaaaGACCAGAAATTAGACAGAACAACTTGCAGTTTATTAAAAATTGAATTGTGTAAAACCACTTTCACTGGAATCCGAGTATCTAATTTAATAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAAc                                        <  1:1802734/138‑1 (MQ=255)
                                                                                                            aagaGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGcc                                 <  1:4405289/140‑1 (MQ=255)
                                                                                                            aagaGAACTGAGACAGAAGAAGATGCAGATTATATACAAATGAATGCTGTAAAAGCACATTCACAGGAACGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGcc                                 <  1:7129636/140‑1 (MQ=255)
                                                                                                             atataaCTTAGACAGAATTATATGAAGATTCTATAAAAATGAATGCTTTAAAAGCACATTCACATGAAAGCGAGTCTCTAATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCa                                <  2:6193891/136‑1 (MQ=255)
                                                                                                             agagAACGGAGGCAGAAGAAGATCCAAGCCATATACAAATGAATGCTGAAATCGATCATTCACAGGAAAGCGATTCTCTCATCGAACAAACGGCACAATTTGTGAAGCATACGCTGGCAAAATCAATCAAACTATCACCa                                <  1:6773378/140‑1 (MQ=255)
                                                                                                               agaACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCaga                              >  1:5346922/1‑140 (MQ=255)
                                                                                                               agaACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCaga                              >  1:7215864/1‑140 (MQ=255)
                                                                                                               agaACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCaga                              >  1:6343776/1‑140 (MQ=255)
                                                                                                                        aCAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCa                     >  1:3291110/1‑140 (MQ=255)
                                                                                                                         cAGAAGAAGATGCAGTTTCTATACAAATGAATGCTTTAAAAGCACATTCACCGGAACGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCAt                    <  1:2472423/140‑1 (MQ=255)
                                                                                                                         cAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAACGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCAt                    <  2:6959987/140‑1 (MQ=255)
                                                                                                                         cAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACACACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCAt                    <  1:1057530/140‑1 (MQ=255)
                                                                                                                         cAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCAt                    <  1:3673051/140‑1 (MQ=255)
                                                                                                                         cAGAAGAAGATGCAGATTATATACAAATGAATGCTGTAAAAGAACATTCACAGGAAAGCGAGTCTCTCATTGAACACACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCAt                    <  1:3891798/140‑1 (MQ=255)
                                                                                                                           gaagtagaTGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATGGAACAAACGACTCATTTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGa                  >  1:399935/1‑140 (MQ=255)
                                                                                                                           gaagaagaTGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGa                  >  1:5533723/1‑140 (MQ=255)
                                                                                                                           gaagaagaTGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGa                  >  1:283432/1‑140 (MQ=255)
                                                                                                                            aagaagaTGCAGATTCTATACAAATCAATGCTGTAAAACCACATACACAGCAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGaa                 <  2:2125894/140‑1 (MQ=255)
                                                                                                                                agaTGCATATTATATACAAATTAATGGTTTATAAGCACATTCACAGTAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTTGCAAAATCGATCAAACTATCTCCAGAGCGTATTCATGAAGATa             <  2:6316420/140‑1 (MQ=255)
                                                                                                                                agaTGCATATACGAGACAAATTCTTGCTGTAAAAGCACATTCCCAGGAAACCGAGTCTCTCATGGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACGATCGCCAGAGCGTATTCATGAAGATa             <  2:1002930/140‑1 (MQ=255)
                                                                                                                                agaTGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTTAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATa             <  1:3014571/140‑1 (MQ=255)
                                                                                                                                agaTGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATa             <  2:6779953/140‑1 (MQ=255)
                                                                                                                                agaTGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAACCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATa             <  2:1340048/140‑1 (MQ=255)
                                                                                                                                agaTGCAGATTATATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATa             <  2:4879656/140‑1 (MQ=255)
                                                                                                                                   tGCATATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGa          <  2:4558048/140‑1 (MQ=255)
                                                                                                                                   tGCAGATTATATACAAATGAATGCTTTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGa          <  1:6136371/140‑1 (MQ=255)
                                                                                                                                      aGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACAt       >  2:2379226/1‑140 (MQ=255)
                                                                                                                                      aGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACAt       >  2:7620594/1‑140 (MQ=255)
                                                                                                                                       gATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACAtt      >  1:881992/1‑140 (MQ=255)
                                                                                                                                        aTTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTc     <  2:873433/140‑1 (MQ=255)
                                                                                                                                        aTTATATAAAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTc     <  2:1929097/140‑1 (MQ=255)
                                                                                                                                           cTATACAATTGATTGTTTTAAAAGCAAATTCACAGGAAAGGCAGTATATCTTTTAAAAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGATCGTATTCATGAAGATACGACATTCgag  <  1:925454/140‑1 (MQ=255)
                                                                                                                                           cTATACAAATGAATGCTGTAAAAGCACATTCACAGGAACGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCgag  <  1:5285934/140‑1 (MQ=255)
                                                                                                                                           cTATACAAATGAATGCTGTAAAAGCACATTCACAGGAACGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCgag  <  1:2765120/140‑1 (MQ=255)
                                                                                                                                           cTATACAAATGAATGCTGTAAAAGCACATTCACAGGAACGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCgag  <  1:1333273/140‑1 (MQ=255)
                                                                                                                                           cTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCTTTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCgag  <  2:2749084/140‑1 (MQ=255)
                                                                                                                                           cTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCGAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCgag  <  1:2904043/140‑1 (MQ=255)
                                                                                                                                           cTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCgag  <  2:657577/140‑1 (MQ=255)
                                                                                                                                           cTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCACATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCgag  <  1:4171714/140‑1 (MQ=255)
                                                                                                                                           cTATAAAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCgag  <  2:318913/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GCGCTTGAGAGTGAGGGATATCGCTATGTTTCTTTTATTGCTGAACAATCACATCAGCTTGGCCAGCAGATCATTGTTGCTGAAAGTGACGGAGTCGTCCGTCAAAAGAAGAGAACTGAGACAGAAGAAGATGCAGATTCTATACAAATGAATGCTGTAAAAGCACATTCACAGGAAAGCGAGTCTCTCATTGAACAAACGACTCAATTTGTGAAGCATACGCTGGCAAAATCGATCAAACTATCGCCAGAGCGTATTCATGAAGATACGACATTCGAG  >  NZ_CP034943/1820593‑1820871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: