Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,194,168 G→T 15.4% Q247K (CAA→AAA)  kdgR ← pectin utilization transcriptional regulator KdgR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,194,1680GT15.4% 820.9 / 46.5 431Q247K (CAA→AAA) kdgRpectin utilization transcriptional regulator KdgR
Reads supporting (aligned to +/- strand):  ref base G (189/160);  new base T (77/3);  total (268/163)
Fisher's exact test for biased strand distribution p-value = 7.48e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

GGACCAATCAGTTTATACCATTCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATACACAGTTGCTCTTTCTGCA  >  NZ_CP034943/2194029‑2194307
                                                                                                                                            |                                                                                                                                           
      aTCAGTTTATACCATTCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCgg                                                                                                                                       >  2:3911539/1‑140 (MQ=255)
          gTTTATACCATTCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCAtc                                                                                                                                   <  2:3861189/140‑1 (MQ=255)
           tttATACCATTCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCTCTTTTTTTTTCTCCGGCAtct                                                                                                                                  >  2:3513741/1‑140 (MQ=255)
           tttATACCATTCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCAtct                                                                                                                                  >  2:2574358/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTTCTCCGGCATGTCTTTGTGaaa                                                                                                                        >  1:3614109/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTGCGGCATCTCTTTGTGaaa                                                                                                                        >  1:7723174/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGtaa                                                                                                                        >  1:2286473/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGaaa                                                                                                                        >  2:5850065/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGaaa                                                                                                                        >  2:4016240/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGaaa                                                                                                                        >  2:2702798/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTGTGTGaaa                                                                                                                        >  1:6905332/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCGGCGGCATCTCtttttttaa                                                                                                                        >  1:3106394/1‑134 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGATGATGATTTTCAGCATAATTCAGCCGTTCAAAGCCAGAATCGCTGTTTTTTTCTCAGGCATGTCTGTGTGaca                                                                                                                        >  2:6310149/1‑138 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGATCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGACTCGCTTTTTTTTGGTACGGCATCTCTTTGTGaaa                                                                                                                        >  2:3328103/1‑140 (MQ=255)
                     tCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGAGACGCAGGCCAAGCTCTTCCCTACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTACGGCATATCTTTGTGaaa                                                                                                                        >  1:2041188/1‑140 (MQ=255)
                                gAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  2:3243871/140‑1 (MQ=255)
                                gAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  1:1073652/140‑1 (MQ=255)
                                gAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  1:847842/140‑1 (MQ=255)
                                gAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  2:5097450/140‑1 (MQ=255)
                                gAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  2:5621140/140‑1 (MQ=255)
                                gAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  1:6089044/140‑1 (MQ=255)
                                gAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  1:5447122/140‑1 (MQ=255)
                                gAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  1:3855762/140‑1 (MQ=255)
                                 aaCCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATTATTTTCAGCATAATTAAGACGTTCAAAGCCAGAATCGCTTTTTTTGCTCCGGCATCTCTTTGTGAAAATAACGAAGCGcc                                                                                                           >  2:461933/1‑140 (MQ=255)
                                 aaCCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcgc                                                                                                            <  2:3403974/140‑1 (MQ=255)
                                 aaCCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcgc                                                                                                            <  1:3242226/140‑1 (MQ=255)
                                 aaCCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcgc                                                                                                            >  2:7128673/1‑140 (MQ=255)
                                      aGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCCCCt                                                                                                       >  2:2239102/1‑140 (MQ=255)
                                           tCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGt                                                                                                  >  2:411945/1‑140 (MQ=255)
                                           tCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGt                                                                                                  >  1:7577405/1‑140 (MQ=255)
                                                atatCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCTTTCAAAGCCAGAATCGCTTTTTTTTCTCCGGCATATATTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATca                                                                                            >  2:2934655/1‑139 (MQ=255)
                                                      tGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTTCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATGCTTGtt                                                                                       >  1:4143523/1‑140 (MQ=255)
                                                      tGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGtt                                                                                       >  2:738991/1‑140 (MQ=255)
                                                      tGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTGTGGGTAAAGGACGAAGCGCCACCGTAAGTTGATACTGGtt                                                                                       >  1:4253251/1‑140 (MQ=255)
                                                       gAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAATCCCGAATCGCTTTTTTTTTCTCCGGCATCTCTTTGTGACAAGAACGAAGAGCCACCTTAAGTTGATCCtttttt                                                                                      >  2:4345051/1‑140 (MQ=255)
                                                       gAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTTCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGttt                                                                                      >  1:5317526/1‑140 (MQ=255)
                                                       gAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTTTGAAAAGAAAGAAGCGCCAACTTAAGTTGATCCATtttt                                                                                      >  1:6008276/1‑140 (MQ=255)
                                                       gAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  1:48210/117‑1 (MQ=255)
                                                       gAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGttt                                                                                      >  2:1038441/1‑140 (MQ=255)
                                                       gAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGttt                                                                                      >  2:3442803/1‑140 (MQ=255)
                                                       gAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCGTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGttt                                                                                      >  2:1522407/1‑140 (MQ=255)
                                                        aGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTTCTACGGCATGTCTTTTTGAAAAGAAACAATCGCCCCCTTAAGTTGATACTtttttt                                                                                     >  1:5555889/1‑140 (MQ=255)
                                                        aGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTGCGGCATCTCTTTGTGAAAAGAACGCAGCGCCACCTTAAGTTGATCCTTGtttt                                                                                     >  1:6269556/1‑140 (MQ=255)
                                                         gggATACTCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGttttt                                                                                    <  2:5243559/140‑1 (MQ=255)
                                                              aCGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCAACTCTTTGTGAAAAGAACGAAGCGCCACCATAAGTTGATCCTTGTTTTTTACga                                                                               >  1:1609537/1‑139 (MQ=255)
                                                               agCAGGCCAAGCAGGTCCATACACCTGATTATTTTCAGCATAATTAACCCGTTCAAATCCATAATAGCTTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGt                                                                               <  1:6652374/139‑1 (MQ=255)
                                                                  aGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAAt                                                                           <  2:3039266/140‑1 (MQ=255)
                                                                    gCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTGTTTTGCTGCGGCCTCGCTTTGTGAAAAGAACGAAGCGCCCCCTTAGGTTGTGGGTTGTTTTTTTGGTCAAttt                                                                         >  1:4924724/1‑140 (MQ=255)
                                                                      cAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCa                                                                       <  2:4726460/140‑1 (MQ=255)
                                                                       aaGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGGATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCaa                                                                      >  2:5964729/1‑139 (MQ=255)
                                                                         gCTCGTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATAc                                                                    <  1:7247109/140‑1 (MQ=255)
                                                                          ctcgTCCATACAGCTGATGATTTTCAGCATAATTCAGCCGTTCAAAGCCAGACTCGCTTTTTTTGGCTCCGGCATCTCTTTGTGACAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACa                                                                   <  2:3711811/140‑1 (MQ=255)
                                                                           tctTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACAt                                                                  <  1:3992751/140‑1 (MQ=255)
                                                                           tcgtCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGACTCGCTTTTTTTTGCTCCGGCATCTCATTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACAt                                                                  <  1:6140388/137‑1 (MQ=255)
                                                                              tCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTTCTCCGGCAGCTCTTTGTGAAAAGAAAGAAGCGCCCCCGTAAGTTGTTGCTTGTTTTTTACGTCAAATTCATAAAtaat                                                               >  1:1027802/1‑137 (MQ=255)
                                                                              tCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCAGGTCTTTGTGAAAAGAACGTAGCGCCACCTTATGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCt                                                               >  1:1858136/1‑140 (MQ=255)
                                                                              tCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCAACTCTTTTTTAAAATAAAGAAACGCCACATTAAGTTTATGCTTTTTTTTTAAGTCAATTTAATACaatat                                                               >  1:1289521/1‑136 (MQ=255)
                                                                              tCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAAACGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAAAGAAGCTCCACCTAAAGTTGATACTTGTTTTTTAAGTCAATTTAATACAttat                                                               >  2:5713083/1‑138 (MQ=255)
                                                                              tCCATACAGCTGATGATTTTCAGCATAATTAAGCCGGTCAATGGCCGAATCGCTTTTGTTTGCTACGGCATCTATGTGTGAAAAGAACGACGCGCCACCTTAAGTTGATCCGTGGTTTTTAGGTCAGTTTCATGCagtgt                                                               >  2:6273935/1‑136 (MQ=255)
                                                                                  tACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACa                                                           <  2:4836902/140‑1 (MQ=255)
                                                                                       cTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGGTGCGGCCTCTATTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGAGGCTTGTTTTTTTCGTAAAATTACTACCATCTTActctgcc                                                      >  1:239406/1‑134 (MQ=255)
                                                                                       cTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCCTCTCGTTGTGAAAAGAACGAAGCGCCACCGTAAGTTGATCCATGGTTTTTACGTCAAGTTCATAAAATATTACAAGccc                                                      >  1:4522473/1‑140 (MQ=255)
                                                                                       cTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAATAAACAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTAATAAATTATTAAAAcccc                                                      >  1:304418/1‑140 (MQ=255)
                                                                                       cTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAATCGCCACCTTAAGTTGATACTTGTTTTTTACGTCAATTTAATAAATTATTaaaatacc                                                      >  2:6114328/1‑136 (MQ=255)
                                                                                       cTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAAt                                                                           <  1:4678427/119‑1 (MQ=255)
                                                                                       cTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAAAGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGccc                                                      >  2:6410510/1‑140 (MQ=255)
                                                                                         gatgatTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTTCTCCGGGATCTCTTTTTTGAAAGAAAGAACCGGCACCATAAGGTGATGCGTGTTTTTTACGTCAATTTGATTAATTATTAAAACCCCGt                                                    >  1:4588749/1‑140 (MQ=255)
                                                                                          atgatTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             <  1:7458144/82‑1 (MQ=255)
                                                                                           tgatTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCGTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTc                                                  <  2:5471483/140‑1 (MQ=255)
                                                                                              ttttCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTTCTCCGGCCTCTCTTTGTGAAAAGAACGAAGCGCCACCATAACTTGATCCTTGTTTTTTACGTCAAATTCATACATTCTTACAAGCCCGGTCGCa                                               >  1:3461914/1‑140 (MQ=255)
                                                                                                 tCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAATAACGAAGCGCCACCTTAAGTTGAGCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCAttt                                            >  2:1888582/1‑140 (MQ=255)
                                                                                                 tCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAgcg                                                                                                             >  2:2479018/1‑75 (MQ=255)
                                                                                                        aaTTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCg                                     >  2:6853963/1‑140 (MQ=255)
                                                                                                        aaTTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCATAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCg                                     >  2:7261299/1‑140 (MQ=255)
                                                                                                        aaTTAAGACGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGAGCCTTGTTTTTTACGTCAATTTCATACATTCTTCCAAGCCCGTTCGCATTTTGGGCCg                                     <  2:1705529/140‑1 (MQ=255)
                                                                                                             aGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCAcc                                                                                                        <  1:5705723/68‑1 (MQ=255)
                                                                                                              gCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCa                               <  1:502928/140‑1 (MQ=255)
                                                                                                              gCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCa                               <  1:1752396/140‑1 (MQ=255)
                                                                                                              gCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTATTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCa                               <  2:1998126/140‑1 (MQ=255)
                                                                                                                cGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCAtt                             <  1:6541041/140‑1 (MQ=255)
                                                                                                                cGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCAtt                             <  2:7521396/140‑1 (MQ=255)
                                                                                                                cGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCAtt                             <  2:1212513/140‑1 (MQ=255)
                                                                                                                cGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCAtt                             <  1:7639592/140‑1 (MQ=255)
                                                                                                                  ttCAAAGCCCGAAACGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGGTGTTTACGACACTGTCATAGATTCGTACAATCCCGTTCGCAGTTTGGGCCGAAGcccgttg                           >  2:4522674/1‑135 (MQ=255)
                                                                                                                  ttCAAAGCCAGAATCGCTTTTTTTTTGTCCGGCCTGTCTTTGTTTAAATAAAGAAGCGCCCCCGTAAGTTTTTACTTTTTTTTTTAGTCAATTTGATACAATATTAAAAACCCGGTGGCATTTTTGGCCGGAACCAttta                           >  1:392003/1‑139 (MQ=255)
                                                                                                                  ttCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAATAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTAAGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTc                           >  2:622632/1‑140 (MQ=255)
                                                                                                                  tacAAAGCCAGACTCGCTTTTTTTTGCTCCGGCAACTCGTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGGGAATTGGATACCATCTTACAAGACCGTTGGCATTTTGGGCCGAATCCATTTc                           >  2:13836/3‑140 (MQ=255)
                                                                                                                   tCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTcc                          <  1:6383525/140‑1 (MQ=255)
                                                                                                                    cAAAGCCAGAATCGCTTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTcc                          <  2:7668761/140‑1 (MQ=255)
                                                                                                                        gCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATa                     <  1:287325/140‑1 (MQ=255)
                                                                                                                        gCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATa                     <  1:674720/140‑1 (MQ=255)
                                                                                                                          cAGAATCGCTTTTTTGTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATaca                   <  2:6045712/140‑1 (MQ=255)
                                                                                                                            gAATCGCTTTTTTTTTGTCCGGCCTCGCTTTGTGAAAAGAACCAACCGCCCCCGTAAGTTGATCCTTGTTTTTTTAGTCAATTTCATAAATTCTTACAAACCCCGTCGCATTGTGTGCCGAAGCCATTTCCTGATAcaca                 >  1:2640403/1‑140 (MQ=255)
                                                                                                                            gAATCGCTTTTTTTTTCTCCGGCATCTCTTTGTGAAAAAAACCAAGCGCCACCTTAAGTTTATCCTTGTTTTTTTCGTCAAATTAATACATTCATACAAGCCCCGTCGCAATTTTGGGCGAAGCCAATTACTGATacaaa                 >  1:4413472/1‑138 (MQ=255)
                                                                                                                              aTCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATACACAGt               <  1:706196/140‑1 (MQ=255)
                                                                                                                                 tcgctttttttGCTCCGTCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATACACAGTTg             <  2:6531756/136‑1 (MQ=255)
                                                                                                                                  cTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATACACAGTTGct           <  2:2122175/140‑1 (MQ=255)
                                                                                                                                  cTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATACACAGTTGct           <  1:2150939/140‑1 (MQ=255)
                                                                                                                                        ttttGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATACACAGTTGCTCTTTc      <  1:6341473/140‑1 (MQ=255)
                                                                                                                                            gCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATACACAGTTGCTCTTTCTGCa  <  1:5870241/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GGACCAATCAGTTTATACCATTCCGTATCATCGAACCCAGCAATCCCTATATCTTGAGGGATACGCAGGCCAAGCTCTTCCATACAGCTGATGATTTTCAGCATAATTAAGCCGTTCAAAGCCAGAATCGCTTTTTTTTGCTCCGGCATCTCTTTGTGAAAAGAACGAAGCGCCACCTTAAGTTGATCCTTGTTTTTTACGTCAATTTCATACATTCTTACAAGCCCGTTCGCATTTTGGGCCGAAGCCATTTCCTGATACACAGTTGCTCTTTCTGCA  >  NZ_CP034943/2194029‑2194307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: