Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 177,011 T→G 8.1% intergenic (+184/‑88) EO946_RS01020 → / → EO946_RS01025 alcohol dehydrogenase/SulP family inorganic anion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943177,0110TG 8.1% 768.1 / 7.3 336intergenic (+184/‑88)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
Reads supporting (aligned to +/- strand):  ref base T (159/131);  new base G (42/4);  total (201/135)
Fisher's exact test for biased strand distribution p-value = 6.01e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 360 total aligned reads displayed.

AGCTCAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCG  >  NZ_CP034943/176872‑177145
                                                                                                                                           |                                                                                                                                       
aGCTCAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGtt                                                                                                                                         <  2:1839502/140‑1 (MQ=255)
  cTCAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGTTCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGtttt                                                                                                                                       >  2:5222523/1‑140 (MQ=255)
  cTCAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGtttt                                                                                                                                       >  2:824228/1‑140 (MQ=255)
  cTCAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGtttt                                                                                                                                       >  1:4480615/1‑140 (MQ=255)
  cTCAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGtttt                                                                                                                                       >  2:4207886/1‑140 (MQ=255)
  cTCAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGtgtt                                                                                                                                       >  2:4791196/1‑140 (MQ=255)
    cAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCACAACAACATACTGAACGTGTCCTCTTTTTATCCGGTCGTTTTcc                                                                                                                                     >  2:224518/1‑140 (MQ=255)
      aaCTAAGTTTGAGCCATTATTTTCTTCAGCATAGATCTTGCGTCATTGATCAAAATATACTATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAAGGTCTCCTCTTTTTCTCCGGTCGtttttgtt                                                                                                                                   >  1:3392735/1‑136 (MQ=255)
            gTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCAttt                                                                                                                             >  2:894949/1‑140 (MQ=255)
                 aGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATAGTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGATTTACTATCAtttatagc                                                                                                                        >  1:7542182/1‑135 (MQ=255)
                 aGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCTTTAAtttttacc                                                                                                                        >  1:226291/1‑135 (MQ=255)
                 aGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTAAtttgtacc                                                                                                                        >  1:566300/1‑135 (MQ=255)
                 aGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTCAattatacc                                                                                                                        >  1:1333101/1‑135 (MQ=255)
                 aGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCAGGTCGTTTTACTTTCAtttatacc                                                                                                                        >  1:4643650/1‑135 (MQ=255)
                     attattTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTAATTTATCCCGTaa                                                                                                                    >  1:5210646/1‑140 (MQ=255)
                      ttattTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCTGTAATTTATCCCGTaaa                                                                                                                   >  1:3316021/1‑140 (MQ=255)
                           ttcttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTAATTTGTACCGTAAAATaaa                                                                                                              >  1:2771338/1‑140 (MQ=255)
                            tcttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTATACCCTaaaaaaaaa                                                                                                             >  2:3296163/1‑139 (MQ=255)
                             cttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATaaaga                                                                                                            <  1:4207886/140‑1 (MQ=255)
                             cttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATaaaga                                                                                                            <  1:170332/140‑1 (MQ=255)
                             cttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATaaaga                                                                                                            <  2:7123579/140‑1 (MQ=255)
                               tcatcaTAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATaaagaaa                                                                                                          <  1:6776874/140‑1 (MQ=255)
                                catcatAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGGTTTGATTTATACCCGAAAAAaaagaaac                                                                                                         >  1:24158/1‑139 (MQ=255)
                                catcatAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAAAaaagaaaa                                                                                                         >  2:92461/1‑139 (MQ=255)
                                catcatAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTAAATTATACCCTAAAATaaaaaaaa                                                                                                         >  1:2133119/1‑139 (MQ=255)
                                   catAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAACCTc                                                                                                      >  2:3401629/1‑140 (MQ=255)
                                       taTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCaa                                                                                                  <  2:5137016/139‑1 (MQ=255)
                                       gATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCaa                                                                                                  <  1:1109654/140‑1 (MQ=255)
                                       gATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCaa                                                                                                  <  2:6713836/140‑1 (MQ=255)
                                        aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                 <  2:5003083/140‑1 (MQ=255)
                                        aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTAATTTATCCCGTAAAATAAAGAAAGCTCCCaaa                                                                                                 >  2:6966027/1‑139 (MQ=255)
                                        aTCTTGCGTAATTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                 <  2:1090783/140‑1 (MQ=255)
                                          cTTTCGTATTTGATGAAAATTTAATATGATACTATTTGTATAATAACTCCTTGACTCAAAACAACATACTCAACGTGTCCTCTTTTTATCCGTTCGTTTTCCTTTAATTTCTCCAGTAAAATAAAGAAAGCTCCCAATac                                                                                               <  2:7080234/140‑1 (MQ=255)
                                          cTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                               <  1:768341/140‑1 (MQ=255)
                                          cTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                               <  1:6979684/140‑1 (MQ=255)
                                          attGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                               <  1:4581275/139‑1 (MQ=255)
                                          attGCGTATTTGATCAAAATTTAAGATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                               <  2:3141519/139‑1 (MQ=255)
                                             gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCTTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACATTCATTTCTCCCGTAAAATAAAGAAATCTCCCAATacaaa                                                                                            >  2:3785363/1‑139 (MQ=255)
                                             gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAACTCACAATaaaaa                                                                                            >  2:1655023/1‑139 (MQ=255)
                                             gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTATACCCTAAAATAAAGAAAGCACCCAATaaaaa                                                                                            >  2:133335/1‑139 (MQ=255)
                                             gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAAAAACTCCTTGCCTCAAAACAACATAATCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTACTAGCGATAAAAAAACAAAGCAACCAAAacaaa                                                                                            >  2:3896171/1‑139 (MQ=255)
                                                tATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTATACCCTAAAATAAATAAAGCTACCAATAAAACaaa                                                                                         >  2:5088040/1‑140 (MQ=255)
                                                tATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACcaa                                                                                         >  2:7198968/1‑140 (MQ=255)
                                                  ttttATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTTAATTATCACCTAAAATAAAAAAAGAACCCAaaaaaaaaaaaa                                                                                       >  1:910909/1‑138 (MQ=255)
                                                  tttGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTTCTTTCATTTATACCCTAAAAAAAACAAAAATCCCAaaaaaaaaaaaa                                                                                       >  1:3140081/1‑138 (MQ=255)
                                                  tttGACCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATa                                                                                       <  1:4824124/140‑1 (MQ=255)
                                                        cAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTGATTTGTCCCGGAAAATAAAGAAATCTCCCAATACAAAAAATaacgttc                                                                                 >  1:4943243/1‑134 (MQ=255)
                                                        cAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTATCCCCTAAAAAAAAAAAAGAACCCCAAACAACAAATAAGGTGc                                                                                 >  1:2966699/1‑140 (MQ=255)
                                                                     gATACTCTTTGTATAATAACTCCTTGCCTCAATACAACATACTCAACGTGTCCTCTTTTTCTCGGGTCGTTTTGCTTTGATTTGTCCCGTAAAATATAGAAAGATCCCAATACAACAAATACGGGGCGGAGTTAtttttt                                                                    >  2:5371991/1‑140 (MQ=255)
                                                                         ctctTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTaaaa                                                                <  1:644308/140‑1 (MQ=255)
                                                                            tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTGTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTTAAAAgg                                                             >  2:1940792/1‑140 (MQ=255)
                                                                             ttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGa                                                            <  2:531423/140‑1 (MQ=255)
                                                                              tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTGATTTTGACCGGAAAAAAAACAAAACAACCAATAAAACAAAAAACGAGCGGGTTTTTTTTTTTAAAATGAg                                                           >  1:6692671/1‑140 (MQ=255)
                                                                              tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTGATTTGTCCCGTAAAATAAAGAAAGCTCCCCATACAACAAATACCGGGCGGATTTATTTTTTAAAAAGGat                                                           >  1:1466920/1‑139 (MQ=255)
                                                                                   aataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTCTCCCGTAAAATAAATAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTGGtt                                                      >  2:4276105/1‑140 (MQ=255)
                                                                                   aataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTTATTTGTCCCCGAAAATAAATAAAGCTCCCAAAACAACAAAGACGGGTGGGATGTATTTTTTTAATAgggggggtt                                                      >  1:1528955/1‑133 (MQ=255)
                                                                                    ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTGTCCCGTAAAATAAAGAAAGCTCCCAATAAAACAAAAACGGGGCGGATTTATTTTTTAAAAAGGAGTGGTta                                                     >  2:6323701/1‑140 (MQ=255)
                                                                                    ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTGATTTCTCCCCTAAAATAAAGAAAGCTCCCAATAAAACAAATACGGTGCGGATTTATTTTTTAAAAAGGATTGGTta                                                     >  2:6598114/1‑140 (MQ=255)
                                                                                     taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCAATTATCCCCTAAAATAAAAAAAACGCCCAAAACAAAAAAAAAAGTGCGGCTTTTTTTTTTAAAAACGAGTAGTtat                                                    >  1:2846633/1‑140 (MQ=255)
                                                                                     taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAATAAAGCTCCCAAAAAAACAAAAACGGTGGGGGTTTTTTTTTTAAAAATGAGTCGTtat                                                    >  2:5577676/1‑140 (MQ=255)
                                                                                     taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTACCAATACAAAAAAAAAGGTGCGGATTTATTTTTTAAAAAGGAGTCGGtat                                                    >  2:3967073/1‑140 (MQ=255)
                                                                                       aCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                 <  1:949562/140‑1 (MQ=255)
                                                                                       aCTCCGTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                 <  2:2883895/140‑1 (MQ=255)
                                                                                        cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTGCCGTAAAATAAAGTAAGCTCCCAATACAACAAAAAACGGGCGGGTTTATTTTTTAAAAAGGTGTGGTTATAtt                                                >  2:3244638/1‑140 (MQ=255)
                                                                                        cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTCTACCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAATGATTAGTtttaat                                                 >  2:7695574/1‑138 (MQ=255)
                                                                                        cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTATCCCCTAAAATAAAGAAAGCTCCCAAAAAAACAAATAACGTGCGGATTTATTTTTTTAAAAGGATTCGTTATAtt                                                >  2:6504673/1‑140 (MQ=255)
                                                                                          ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCGTTAATTTATCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGGGGATTTATTTTTTAAAAAGGGGGCGGTGTAtttt                                              >  2:3059830/1‑139 (MQ=255)
                                                                                          ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTATCCCGTAAAATAAAGAAAAATCCCAATAAAAAAAATAAGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTg                                              >  2:2272991/1‑140 (MQ=255)
                                                                                          ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACGGTGATTTATCCCGGAAAAAAAAAAAAGCTCCCAAGAAAACAAAAAAGGTGGGGATTTATTTTTTTAAAAGGGGTGGTTGTAtttt                                              >  1:4750237/1‑139 (MQ=255)
                                                                                             tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTGTGCCGTAAAATAAAGAAAGCTGCCAATACAACAAATACGGGGCGGCTTTATTTTTTTAAAAGGAGTCGGGATTTTTGtat                                           >  1:5297506/1‑140 (MQ=255)
                                                                                             tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAATAAACAAACCTCCCCATACAACAAAAAACGTGCGGAGTTATTTTTTAAAAAGGAGTACTTGTATTTgtaa                                           >  2:5939207/1‑137 (MQ=255)
                                                                                              gCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaata                                          <  1:2471547/140‑1 (MQ=255)
                                                                                               ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                         <  1:3719287/140‑1 (MQ=255)
                                                                                               ccTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaataa                                         <  2:3499627/140‑1 (MQ=255)
                                                                                                   aaaaCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTAATTTGTCCCGTAAAATAAAGTAAGCTCCCCATAAAAAAAATAAGGGGCGGATTTATTTTTTAAAAATGTGTCGTTATATTTGTATAAAAGt                                     >  2:645031/1‑140 (MQ=255)
                                                                                                     aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                   <  1:5532173/140‑1 (MQ=255)
                                                                                                     aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                   <  1:5987027/140‑1 (MQ=255)
                                                                                                     aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                   <  2:6189112/140‑1 (MQ=255)
                                                                                                          gctccTCATCGTGTCCTCTTTTTCTCGGGTCGTGTTCCTTTCTTTTCTCCCGTAAAATTAAGACAGCTCACAATACAACAACTTCGGTGCGGATTTATTTTTTAAAAAGGTGTCGTTATATTTGAATAATAGTTTTACAt                              <  2:7583207/136‑1 (MQ=255)
                                                                                                          caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                 >  2:1463955/1‑74 (MQ=255)
                                                                                                          caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACAt                              <  2:4480615/140‑1 (MQ=255)
                                                                                                          caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACGTTGAATTCTACCCTAAAATAAAGAAAACACCCAAAACAAAAAAAAAGGGGCGGATTTATTTTTTAAAAAAGAGTCGTGAAATTTGAATAATAATATTACAt                              >  2:6267507/1‑140 (MQ=255)
                                                                                                          caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACGTTACTTTCTCCCGTAAAATAAATAAAGCGCCCAATACAAAAAATACGGTGGGGATTTATTTTTTTAAAATGAGGGGTTATTTTTTTATAATTTTTTTAAAt                              >  2:3719287/1‑140 (MQ=255)
                                                                                                          caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACGTTAATTTATACCGTAAAATAAAGAAACCTCCCAATAAAACAAAAACCGTGCGGATTTATTTTTTAAAAATGAGGCGGTATATTTTAATAATATTTTTAAAt                              >  2:208086/1‑140 (MQ=255)
                                                                                                          caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCGTAAAAAAAAGAAATCTCCCAATAAAACAAAAAGGGTGCGGATTTATTTTTTAAAAAGGAGTGGGTATATTTTAATAATAGgttttaat                              >  2:4404202/1‑136 (MQ=255)
                                                                                                                 aaCGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTAATTTCTCCCCTAAAATAAAGAAATCTGCCAATACAAAAAATAAGGTGCGGATTTATTTTTTAAAAAGGAGTCGGTTTATTTTGATAATAGGTGTACATTAAagac                       >  2:4966388/1‑138 (MQ=255)
                                                                                                                 aaCGTGTCCTCTTTTTCTCCGGTCGTTTTCCGTTGATTTCTCCCGTAAAATAAAGAAAACTCCCAATAAAACAAATAAGGTGCGGATTTATTTTTTAAAAAGGAGTAGTTATATTTTAAATAATTTTTTAAATTAAagga                       >  2:2722520/1‑138 (MQ=255)
                                                                                                                    gtgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAgcagca                    <  1:6298061/140‑1 (MQ=255)
                                                                                                                    gggtCCTCTGTGTCTCCGGTCGTTTTCCTTTCATGTCTCCCCTAAAACAAAGAAAGCTCCCAATCCCACAAATACGGTGCGGAATTATTTTTTAAAAAGGAGACGTTATATTTGAATAATAGTTTTACATTAAAgcagca                    <  1:4057805/138‑1 (MQ=255)
                                                                                                                     tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACACATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                   <  1:6391409/140‑1 (MQ=255)
                                                                                                                     tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                   <  2:6088213/140‑1 (MQ=255)
                                                                                                                     tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                   <  2:6869006/140‑1 (MQ=255)
                                                                                                                     tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                   <  1:7223935/140‑1 (MQ=255)
                                                                                                                     tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                   <  1:141896/140‑1 (MQ=255)
                                                                                                                     agacagctTTTTCTCCTGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                   <  1:5850208/134‑1 (MQ=255)
                                                                                                                       tCCTCTTTTTCTCCGGTCGTTTTGCTGTGATTTGTCCCGTAAAATAAATAAAGCTCCCAAAAAAAAAAATAAGGGGGGGATTTATTTTTTTAAAATGAGTGGGTTTTTTTTTATAATATTTTTTAATTAAATCACCAATg                 >  1:7638040/1‑140 (MQ=255)
                                                                                                                          tctTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGtt              <  1:7198968/140‑1 (MQ=255)
                                                                                                                           ctTTTTCTCCGGTCGTTTTCCTTTCATTTCTGCCGTAAAATAAAGAAAGCTCCCAATAAAAAAAATAAGGTGCGGATTTATTTTTTAAAAAGGGGTGGTTTTATTTTAATAATAGTTTTTGATTATATCATCAATGTTTc             >  2:7317769/1‑140 (MQ=255)
                                                                                                                                      gTCGTTTTCCTTTCATTTGTCCCCGAAAAAAAAGAAAGCTACCAAAAAAAAAAATAAGGTGGGGATTTATTTTTTAAAAAGGAGTCGTTTTAATTTAAAAAAAGTTTTAAATTAAATAACCAATGGTTTGGGAATATccc  >  2:1234524/1‑139 (MQ=255)
                                                                                                                                      gTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCg  <  2:24158/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
AGCTCAAACTAAGTTTGAGCCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCG  >  NZ_CP034943/176872‑177145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: