Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,387,066 T→G 13.5% S354R (AGC→CGC)  EO946_RS12195 ← acetyl‑CoA C‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,387,0660TG13.5% 806.5 / 9.8 392S354R (AGC→CGC) EO946_RS12195acetyl‑CoA C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (142/162);  new base G (73/2);  total (228/164)
Fisher's exact test for biased strand distribution p-value = 1.75e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 392 total aligned reads displayed.

CCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAC  >  NZ_CP034943/2386929‑2387204
                                                                                                                                         |                                                                                                                                            
          cTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAAAAATTGTCATGAGTATTATCGCACCGCTTGCGCCGATCgg                                                                                                                                  >  2:6318975/1‑140 (MQ=255)
          cTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAAAAATAGTCATGAGGATTCTCGCACCGCGTGCGCCGCTCgg                                                                                                                                  >  1:3410088/1‑140 (MQ=255)
           tGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCCGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAACTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCggg                                                                                                                                 <  2:5595053/140‑1 (MQ=255)
           tGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCggg                                                                                                                                 <  2:2836503/140‑1 (MQ=255)
           tGTTTGAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCggg                                                                                                                                 <  2:4245046/140‑1 (MQ=255)
                  aTGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGa                                                                                                                          >  2:2753002/1‑140 (MQ=255)
                      aCTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGc                                                                                                                      <  1:3844158/140‑1 (MQ=255)
                       cTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAATTTCATGAGTATTATCGCCCCCCGTGCGCCGATCGGGTGGCCGAGGGCg                                                                                                                     >  1:5049235/1‑140 (MQ=255)
                       cTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTAGTAAACAAGTGTGAAGTGTAATCTCTCACCGCGTTCGCCGCTAGGGTGGCCGTggggg                                                                                                                     >  1:557602/1‑138 (MQ=255)
                        ttGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGa                                                                                                                    <  1:1554271/140‑1 (MQ=255)
                         tGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGAt                                                                                                                   <  2:6254559/140‑1 (MQ=255)
                               aaGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcacc                                                                                                            <  2:352977/140‑1 (MQ=255)
                                aGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACACATGTCATGAGTAATATCGCACCGCTTTCCGCGAACCGGTGGCCGAGGGCGCTCGcacca                                                                                                           >  1:4762922/1‑140 (MQ=255)
                                aGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAAGTCGTAAACAACGGTCAAGAGTCATCTAGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGCTCGcacca                                                                                                           >  2:1142941/1‑140 (MQ=255)
                                      cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTCAGGAGTATTCTCGCCCCGCTTGCGCCCATCGGGTGGCCGCGGGCGAGCGCACCACCGgtt                                                                                                    >  2:76631/1‑140 (MQ=255)
                                            ccTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTGGTAAAAAACTGTGATGAGTATTCTCGCACCGCGTGGGCCGGTCGGGTGGGCGAGGGCGATCGCACCCCCGGTTGCAttt                                                                                              >  1:2372561/1‑140 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATTAGTATTCTCGCACCGCTTGCCCCGATCCGGTGGCCGAGGGCGATCGCACCACCGTGTAAATTTAAtttttaa                                                                                     >  2:6842443/1‑138 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCa                                                                                     >  2:126886/1‑140 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCa                                                                                     >  2:4389162/1‑140 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGGGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCa                                                                                     >  2:5786703/1‑140 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCGTGCGCCGATCGCGTGGCCGAGGGCGCTCGCACCACCGGTTACATTTACTTTTTCa                                                                                     >  2:7151130/1‑140 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAACTGTAATGAGGATTGTCGCACCCCGTGCGCCGCTCGGGTGGGCGAGGGCGATCGCACCCCCCGTTGACGTTACtttttaa                                                                                     >  1:6048274/1‑138 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGGAAACAAGTGTAATGAGTATTATCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCa                                                                                     >  2:2619384/1‑140 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAAAAAGTGTGATGAGTATTATACCACCCCGTGCGGCGATCGGGTGGGCGAGGGCGCTCGCACCCCCGGTTGCCTTTACTTTTTCa                                                                                     >  2:4124885/1‑140 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGGAAACAACTGTCCATAGTATTCGGCCAACGCGTGCGCCGAACGGGTGGCCGAGGGCGAGAGCACCAACGGTTACAATTACattttaa                                                                                     >  2:2280838/1‑138 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTCGTAAAAAAGTGGCATTAGGATTCTCGGACCGCGTGCGCCGCTCGGGTGGCCGCGGGCGCTCGCACCCCCGGTTAGATTTACTTTTTCa                                                                                     >  1:7146459/1‑140 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTATTTAGTAAAGAAGTTTGATGAGTATTGTCGCACCGCGTGCGCCGATCGGGGGGGCGAGGGCGATCGCCCCCCCGGGTACAATTAAtttttga                                                                                     >  1:2726630/1‑138 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTGGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGCGGGCGATCGCACCCCCGTGTACATTTACtttttaa                                                                                     >  2:5900964/1‑138 (MQ=255)
                                                     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAACAAGTGTGATGAGTATTGTCGCACCGGGTGGGCCGATCGGGTGGGCGCGGGCGATCGCACCACCGGTTACATTTTATTTTTCa                                                                                     >  1:5411285/1‑140 (MQ=255)
                                                      aCCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAACTGTCAAGAGTATTCTCGCACCGCTTGCGCCGATCCGGTGGCCGCGGGCGATCGCACCACCGTTTACATTTACTTTTTCaa                                                                                    >  2:1221539/1‑140 (MQ=255)
                                                          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACACGTGTCATGAGTATTCTCGCACCGCTTGCGCCGAGGGGGTGGCCGAGGGCGCTCGCACCCCCGGTGAGCTTTTCTTTTTCAAgtgc                                                                                >  2:5999803/1‑137 (MQ=255)
                                                          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAAAAACTGTCATGAGTAATATGGCACCCCATGGGCCGATCGGGTGGCCGAGGGCGAGGGCCCCCCCGTTTACATTTACTTTTTGAAGATc                                                                                >  1:2166395/1‑140 (MQ=255)
                                                          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTCGTAAACAACTGTCCTGAGTATTCTCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCCGTTACATTTTATTTTTAAAGATc                                                                                >  1:6150845/1‑140 (MQ=255)
                                                               aaTGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGGGTCATGAGTATTCTCGCACCCCGTGCGCCGGGCGGGTGGCCGTGGGCGATCGCACCCCCCGTTCCCATTAAATTTTCAATATCGAAAc                                                                           >  1:7417575/1‑140 (MQ=255)
                                                                  ggcggcAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAACTGTCATGAGTATTATCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTACATTTAATTTTTCAAGATAGAAAAcga                                                                        >  2:1395507/1‑140 (MQ=255)
                                                                     ggcAACACCCAGTCCCCCTCCCCGTCGCGTTTATTCGTAAACAAGTGGGTTTTGTTTTGTAGCACCGCGTGCGGCGCTCGGGGGGCCGGGGGCGCTCGCACCACCCGTTTACTTTAATTTTTCAAGTTCGTAACcgacga                                                                     >  1:7054489/1‑140 (MQ=255)
                                                                     ggcAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGGCATGAGGATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATAGCACCACCCGTTACATTTACTTTTGCAAGTTCGAAACcgaaga                                                                     >  2:1934935/1‑140 (MQ=255)
                                                                             ccAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTa                                                             <  1:5755273/140‑1 (MQ=255)
                                                                             ccACTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTa                                                             <  1:6817754/140‑1 (MQ=255)
                                                                                 tCCCCCTCCCCGTCGCTTTAATTCGTAACCCAGTGTCATGAGTATTCTCCCAACCCTTGCCCCGATCGGGTGGCCGCGGGCGCTCGCACCACCGTTTACATTTAATTTTTCAAGATAGAAACCGCCGATTTTTTTACATg                                                         >  2:811760/1‑140 (MQ=255)
                                                                                 tCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCGGGCGCCGATAGGGTGGCCGAGGGCGATCGCCCCACCGGGTGCAATTACTATTTCAAGATCGAAACCGACGATTTTTTTaaaag                                                         >  2:5000078/1‑136 (MQ=255)
                                                                                     ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa                                                     <  1:4309/140‑1 (MQ=255)
                                                                                     ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa                                                     <  1:5093621/140‑1 (MQ=255)
                                                                                     ccTCCCCGTCGCTTTAATTCGTAAAAAATTGTCATTAGTATTCTCGCACCGCTTGCGCCCATCGGGTGGCCGAGGGCGATCGCACCCCCGTTTACATTTTCTTTTTAAAGATCGAAACCGACGATTTTTTTAAATGTCaa                                                     >  2:4569362/1‑140 (MQ=255)
                                                                                          cggTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaa                                                <  2:6171827/138‑1 (MQ=255)
                                                                                          ccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaa                                                <  2:3465347/140‑1 (MQ=255)
                                                                                           cGTCGCTTTAATTAGTAAACAAGTTTCAAGAGTATTCTCGCACCGCGTGCGCCCATCGGGTGGCCGCGGGCGATCGCACCCCCGGTTACATTTAATTTTTCAAGATCGAAACCGACGATTTTTTTAAATGTCAAaacaaa                                               >  2:3232271/1‑139 (MQ=255)
                                                                                                cTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCCTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATTGCAAAACAACAGAAg                                          >  2:5254082/1‑140 (MQ=255)
                                                                                                  ttAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCa                                        <  2:7338839/140‑1 (MQ=255)
                                                                                                   tAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTAATTTTTCAAGATCGAAACCGACGATTTTTTTAAATGTCAAAACAACAGAAGCaa                                       >  2:2477090/1‑140 (MQ=255)
                                                                                                   tAATTCCTAAACAACTGTCATGTGTATTCTGGCACCGCATGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTACATTTACTTTTTCAAGATCGAAACCTACGATTTTTTTGCATGTCAAAACAACAGAAGccc                                       >  2:6702535/1‑138 (MQ=255)
                                                                                                          tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTc                                >  2:5479676/1‑140 (MQ=255)
                                                                                                          tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTc                                >  2:6520044/1‑140 (MQ=255)
                                                                                                          tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTc                                >  2:4779994/1‑140 (MQ=255)
                                                                                                          tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTAAAGATCGAAACCGACGATTTTTTTACATGTCAAAAAAACAGAAGCAAACGATTc                                >  1:7570189/1‑140 (MQ=255)
                                                                                                            aaCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAt                              <  2:3853430/140‑1 (MQ=255)
                                                                                                            aaCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGGGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTGTACATGTCAAAACAACAGAAGCAAACGCTTCAt                              <  1:4544628/140‑1 (MQ=255)
                                                                                                             aCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAtt                             <  1:1125014/140‑1 (MQ=255)
                                                                                                                  cgtCATGAGAATTCTCGCACCGCTCGCGCCGATCGGGTGGCCGAGCGCGATCGca                                                                                                               >  2:3273785/2‑55 (MQ=255)
                                                                                                                  cgtCATGAGAATTCTCGCACCGCTCGCGCCGATCGGGTGGCCGAGCGCGATCGca                                                                                                               <  1:3273785/54‑1 (MQ=255)
                                                                                                                    tCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCa                      >  2:5725321/1‑140 (MQ=255)
                                                                                                                    tCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACAATTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGTAGCAAAAGCTTCATTTACTTCa                      >  2:7694212/1‑140 (MQ=255)
                                                                                                                      aTGAGTATTCTCGCACCGCTTGGGCAGATCGGGTGGCCGAGGGCGATCGCACCACCTTTTACATTTAGTTTTTCATGATCGAAACCGACGATTTTTTTTCATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTaaaa                    <  1:943897/140‑1 (MQ=255)
                                                                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                    <  1:1055970/140‑1 (MQ=255)
                                                                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                    <  2:181520/140‑1 (MQ=255)
                                                                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                    <  1:5971927/140‑1 (MQ=255)
                                                                                                                         aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACaa                 >  1:4071357/1‑140 (MQ=255)
                                                                                                                         aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACaa                 >  2:6363913/1‑140 (MQ=255)
                                                                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt               >  2:7083413/1‑140 (MQ=255)
                                                                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt               >  1:6391857/1‑140 (MQ=255)
                                                                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt               >  2:3747283/1‑140 (MQ=255)
                                                                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt               >  1:1810457/1‑140 (MQ=255)
                                                                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt               >  1:1659615/1‑140 (MQ=255)
                                                                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt               >  1:3627742/1‑140 (MQ=255)
                                                                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTGTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt               >  2:1693596/1‑140 (MQ=255)
                                                                                                                             ttCTCTCACCGCTTGCGCCGATCGGGTGGCCGAGGGAGATCGCACCACCGTTTACATTTACTTTTTCAATATCTAAACCGACGATTTTTTTACATGTCAAATCAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  1:6976897/140‑1 (MQ=255)
                                                                                                                             ttCTCGGACCGCTTGCGCCGATCGTGTGGCCGAGGGCTATCGCAACACCGTTTAAATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAAGAGAAGCAAACGCTTCATTTAGTTCAAACAAGTCa             <  1:2280838/140‑1 (MQ=255)
                                                                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCTTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  2:4762922/140‑1 (MQ=255)
                                                                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             >  1:7658636/1‑140 (MQ=255)
                                                                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  1:2777290/140‑1 (MQ=255)
                                                                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  1:3126715/140‑1 (MQ=255)
                                                                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  2:7417575/140‑1 (MQ=255)
                                                                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  2:3653574/140‑1 (MQ=255)
                                                                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  1:4387332/140‑1 (MQ=255)
                                                                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  1:6497768/140‑1 (MQ=255)
                                                                                                                              tctcGCACCGCTTTCGCCGATCGGGTTGCCGATTGCGATCGCACCACATTTTACATTTACTTTTTAAAGATAGAAACCGAATATTTTTTTACATGTCAAAACAACATAATAAAAAGATTCATTTACTTCAAACCAGTaaa            <  2:3086761/140‑1 (MQ=255)
                                                                                                                              tctcGCACCGCTTGCGCCGATAGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATATTTTTACATGTCAAAACAACATAAGCAAACGCTTCATTTACTTCAAACAAGTCaa            >  1:3252357/1‑140 (MQ=255)
                                                                                                                              tctcGCACCGCTTGCGCAGATCGGGTGTCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTAAATATATAAACAGACTATTTTTTTACATGTCAAAACAAGATTATTAAAATATTCATTTACTTCaaaaaattaaa            <  2:2217837/140‑6 (MQ=255)
                                                                                                                                tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAata          >  2:6107629/1‑140 (MQ=255)
                                                                                                                                tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAata          >  1:2030305/1‑140 (MQ=255)
                                                                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     >  2:7510496/1‑140 (MQ=255)
                                                                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     <  2:6222306/140‑1 (MQ=255)
                                                                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     <  2:6443661/140‑1 (MQ=255)
                                                                                                                                       gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa   <  1:126886/140‑1 (MQ=255)
                                                                                                                                       gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa   <  2:2067455/140‑1 (MQ=255)
                                                                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAacaaca                                              <  2:1885416/96‑1 (MQ=255)
                                                                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa   <  1:5537454/139‑1 (MQ=255)
                                                                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc  >  1:2592977/1‑140 (MQ=255)
                                                                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc  >  2:1551445/1‑140 (MQ=255)
                                                                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTGTACATGTCAAATCAACAGAAGCTAACGCTTCATTTACTTCAAACAAGTCAAGATCTTGGAc  >  1:5880018/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                            
CCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAC  >  NZ_CP034943/2386929‑2387204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: