Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,901,671 A→C 15.4% K411T (AAA→ACA)  EO946_RS14945 → extracellular solute‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,901,6710AC15.4% 752.1 / 47.1 316K411T (AAA→ACA) EO946_RS14945extracellular solute‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (236/21);  new base C (2/57);  total (238/78)
Fisher's exact test for biased strand distribution p-value = 2.30e-42
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

ATGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCGTT  >  NZ_CP034943/2901533‑2901809
                                                                                                                                           |                                                                                                                                          
aTGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGCAGAGAATACaaa                                                                                                                                           >  2:2694978/1‑140 (MQ=255)
aTGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACaaa                                                                                                                                           >  1:3434683/1‑140 (MQ=255)
  gTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAAGGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGACTACAAACg                                                                                                                                         >  2:7003854/1‑140 (MQ=255)
  gTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACg                                                                                                                                         >  2:2095213/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                        >  1:4049744/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                        >  1:2703380/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                        >  2:4772316/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGCCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATGTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                        >  2:3614413/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAAGGTGGGATGTCCTGGGCCCGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACgc                                                                                                                                        >  2:4029431/1‑139 (MQ=255)
            aGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCTAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAg                                                                                                                               >  2:4620533/1‑140 (MQ=255)
             gCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACCGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATCCAAACGACTCCGGGAGc                                                                                                                              >  1:5173595/1‑140 (MQ=255)
                 tACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATc                                                                                                                          >  1:182130/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGACGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:8435/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  2:4746583/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:239509/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:2140530/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  2:5302899/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:3719644/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  2:1429172/1‑140 (MQ=255)
                            tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                               >  2:4297068/1‑140 (MQ=255)
                            tgtataCTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGACTACAAACGACTCCGGGAGCACTCGGAAACACAgg                                                                                                               >  1:2293015/3‑140 (MQ=255)
                              tataCTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGa                                                                                                             >  2:5208956/1‑140 (MQ=255)
                                    tGCCAAACGGAATGTGGGCTGTCCTGGGCACGGTCGGCCAGGAGCTTATGGCTGACATTTTGAAGCCTCAAGAGATTTCGAATAATCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACCGGGAGCTgaa                                                                                                       >  1:7685843/1‑140 (MQ=255)
                                     tccAAACGGAATGTGGGATTTACTTTTAAAGATCCGACATTATCTTATGGCTGACATTTTGACGCCTCAATAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  2:2414357/2‑140 (MQ=255)
                                     gCCCAACGGAAGGTGGGTTGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACCTTTTGCCGCCTCACGAGATTTCTGAGTCGCTGGGACGAGCGCACAAACGCCTCCTGTAGCACTCGTACACACAGGGAGCGgaag                                                                                                      >  2:5778813/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:3195869/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:2486460/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:960611/1‑140 (MQ=255)
                                     cccAACCGGAATGTGGGTGGTCGTGGGCCGGGGCGGACCGGAGTTTTTGGCTTACATTTTGACGCAAAAAGATATTTCTAAGACGCTGGCAATACAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      <  2:2953057/139‑1 (MQ=255)
                                      ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  1:1159827/1‑140 (MQ=255)
                                      ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  2:346441/1‑140 (MQ=255)
                                       cAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaagaa                                                                                                    >  1:739576/1‑140 (MQ=255)
                                         aaCGTATTGTGTGATTTCCGGGGCCCGGTCGGACAGTTGTTTATGGCTGAATTTTTGCCGCCTAAAGATATTTCCATTTAGCTGGTATGATATTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACa                                                                                                  <  2:200899/140‑1 (MQ=255)
                                                    ggttGTCGTGGGCACGGGGGGACAGGAGGTTTTGGCTGAATTTTTGACGCATAAAGATATTTCGAAGAAGCTGGGAAGTTAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGAt                                                                                       <  2:7760294/137‑1 (MQ=255)
                                                        gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:6514329/1‑140 (MQ=255)
                                                        gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:1660966/1‑140 (MQ=255)
                                                        gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:6607071/1‑140 (MQ=255)
                                                        gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:134035/1‑140 (MQ=255)
                                                        gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:7177322/1‑140 (MQ=255)
                                                        gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAAAACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:3063325/1‑140 (MQ=255)
                                                            tGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAagag                                                                               >  1:6287141/1‑140 (MQ=255)
                                                            tGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAagag                                                                               >  1:6868757/1‑140 (MQ=255)
                                                                cccGGTCGCACAGTCGCTTTGGGCTGAAATTTGGCCGCCAAAACAGATTTCCAACACGCTGGGAAGACAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGt                                                                           <  2:5942570/138‑1 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGACTACAAACGACTCCGGGAGCCCTCGGAAACACAGGGAGCTGAAGACCAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:2902125/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGACTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:289996/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGAAACACAGGGAGCTGAAGAACAATGAGAGAGATCGCAAGAGACGTACa                                                                        >  2:5037490/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:1655614/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:6061351/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:6347924/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:4209983/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:2755297/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:6547149/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:7574402/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:6052284/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACACGGAGCTGAAGAACAATGAGTGAGATCGCAAGATACGTTCa                                                                        >  2:4946757/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTACGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGCGTGAGATCGCAAGAGACGTTCa                                                                        >  2:5233461/1‑140 (MQ=255)
                                                                    gTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAt                                                                       >  2:2562732/1‑140 (MQ=255)
                                                                     tCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAtg                                                                      >  1:5477291/1‑140 (MQ=255)
                                                                     tCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAtg                                                                      >  1:5375546/1‑140 (MQ=255)
                                                                     tCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAtg                                                                      >  2:5513674/1‑140 (MQ=255)
                                                                             gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAg                                                              >  1:1253742/1‑140 (MQ=255)
                                                                             gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAg                                                              >  2:738548/1‑140 (MQ=255)
                                                                               gCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCAGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGt                                                            >  2:1809558/1‑140 (MQ=255)
                                                                                  tATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGaa                                                         >  1:4155837/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGAAACACAGGGAGCTGAAGAACCATGAGTGAGATCGCACGAGACGTTCATGTGAAACAGGTGAAg                                                        >  2:4462710/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                        >  1:3115281/1‑140 (MQ=255)
                                                                                       cttactttttGACGCCTCAATATTTTTCGAATACGGTGGTAAGATAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGa                                                    <  2:7302514/134‑1 (MQ=255)
                                                                                       cTGACTTTTTGCCGCCTCAAGAGATTTCCAAGAAGGTGGTAAGATAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGa                                                    <  2:5697950/140‑1 (MQ=255)
                                                                                          aCATTTTGACGCGTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                                 >  1:5945439/1‑140 (MQ=255)
                                                                                          aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                                 >  1:5213859/1‑140 (MQ=255)
                                                                                          aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                                 >  1:2107153/1‑140 (MQ=255)
                                                                                          aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAACAGAAAACAAACCACTCCGGGAGCAATCGGAAACACAGGCAGCTGAAGAACAATCAGTGACATCGCAAGAGACGTTCATGTGAAAAAGGTGAACCCGaaaa                                                 >  2:4853757/1‑140 (MQ=255)
                                                                                           cATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGACCAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaaa                                                >  2:214818/1‑140 (MQ=255)
                                                                                            aTTTTGACGCATAAAGAGATTACGATTATGTGGGAACGCCAAAACCCACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAg                                               <  1:5276337/140‑1 (MQ=255)
                                                                                               ttGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAg                                            >  2:3294751/1‑140 (MQ=255)
                                                                                                tGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGt                                           >  2:3812949/1‑140 (MQ=255)
                                                                                                      cTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                     >  1:6424991/1‑140 (MQ=255)
                                                                                                           cagaTTTCCAACATGGGGGTAAGACAACACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtgg                                <  2:413383/139‑1 (MQ=255)
                                                                                                             tttttCGAACATGCGGGCAATATAAAAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtg                              <  1:4383257/138‑1 (MQ=255)
                                                                                                              aTTTCTAAGAAGCGGGCAACAGAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  1:5642034/140‑1 (MQ=255)
                                                                                                              aTTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             >  2:2988895/1‑140 (MQ=255)
                                                                                                              aTTCCGAACACGGTGGGAATACAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  1:1040958/140‑1 (MQ=255)
                                                                                                              aTTACGAAGATGGGGGGACGACAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  2:5547520/140‑1 (MQ=255)
                                                                                                                ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           >  1:580662/1‑140 (MQ=255)
                                                                                                                ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           >  2:6895607/1‑140 (MQ=255)
                                                                                                                ttCCAACAAGGTGTGAATACAACACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           <  2:6804801/140‑1 (MQ=255)
                                                                                                                   gaagaaGCTGGGATGATAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGta                        <  2:3758916/140‑1 (MQ=255)
                                                                                                                          cTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATAGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTatatctgt                 >  1:3023817/1‑136 (MQ=255)
                                                                                                                           tGGGAAGAGAAAACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTg                <  2:1940384/140‑1 (MQ=255)
                                                                                                                               aaGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCttt            <  2:6287141/140‑1 (MQ=255)
                                                                                                                                atcagaATAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCttt            <  1:4801155/137‑1 (MQ=255)
                                                                                                                                  gagaATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGc          >  2:4413220/1‑140 (MQ=255)
                                                                                                                                   ataaTAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGct         <  2:1189359/138‑1 (MQ=255)
                                                                                                                                     taaacacacGTCTCCTGGAGCAATCGGAAACACAGGGAGCTGAAGAACATTGATTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:7146406/137‑1 (MQ=255)
                                                                                                                                     accacacacGACGCCGGGAGCAATCGGACACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:6596289/137‑1 (MQ=255)
                                                                                                                                     aaaacacacGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGCGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  2:2261888/137‑1 (MQ=255)
                                                                                                                                     aaaacacacGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  2:162270/137‑1 (MQ=255)
                                                                                                                                     aaaacacacGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:5014049/137‑1 (MQ=255)
                                                                                                                                     aaaaacaaCGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  2:2755297/134‑1 (MQ=255)
                                                                                                                                      aTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCtctc      >  1:1540055/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ATGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCGTT  >  NZ_CP034943/2901533‑2901809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: