BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,991,245	G→T	22.9%	intergenic (+46/‑161)	gbsR → / → bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,245	0	G	T	22.9%	389.0 / 46.8	236	intergenic (+46/‑161)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base G (67/108); new base T (58/3); total (125/111)
Fisher's exact test for biased strand distribution p-value = 2.50e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAA  >  NZ_CP034943/2991109‑2991384

ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATTTaa                                                                                                                                             >  2:1985030/1‑140 (MQ=255)

ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTTTTTaa                                                                                                                                             >  1:5616726/1‑140 (MQ=255)

 tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAAt                                                                                                                                            <  2:1487617/140‑1 (MQ=255)

   tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTCTTTTTTATGTAATta                                                                                                                                          >  2:4510702/1‑140 (MQ=255)

      aGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATtacta                                                                                                                                       <  2:5405024/140‑1 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACACAGTAAAACAAAATCGCCTGGGAACCCGGGCGT‑TTTTTTGGTGTTTTTTATTTAATTACAAC‑aaa                                                                                                                                  >  2:1372476/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTATTTTTTTTTTAATTAAAAC‑aaa                                                                                                                                  >  2:6110633/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATTTAATtactaacaa                                                                                                                                   >  2:4694147/1‑136 (MQ=255)

           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAATCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTATTTTTTTTTTAATtaataacaat                                                                                                                                  >  2:6656125/1‑135 (MQ=255)

           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATTTAATTACTAC‑AAAt                                                                                                                                 >  2:2133412/1‑140 (MQ=255)

           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATGTAATTAATAa‑aaaa                                                                                                                                 >  1:7395753/1‑139 (MQ=255)

           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATGTAATTAATAC‑AAAt                                                                                                                                 >  2:2085008/1‑140 (MQ=255)

           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTATTTAATTAATAa‑aaaa                                                                                                                                 >  1:6946943/1‑139 (MQ=255)

           aCTATGATTGGCTCAAACGGCTTGCTTAATCGGTGAAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAAt                                                                                                                                 <  1:4694147/140‑1 (MQ=255)

                gATTGGCGTAACCGGCGTGCTGCATCGTGGCCGAGCGGGTAAATTTTTAAATTTACACCCATCGAAACAACCGACACAACAAACGCCTGGGAAACCGGGAGT‑TTTTTTGTACATTTTTATGGAATTACTAC‑AAATTTG‑aa                                                                                                                           <  1:533258/140‑1 (MQ=255)

                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATGTAATTAATAA‑AAATTTT‑aaa                                                                                                                          >  2:2021991/1‑140 (MQ=255)

                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTT‑aaa                                                                                                                          >  2:2418793/1‑140 (MQ=255)

                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTATTTTTTATGTAATTAATAC‑AAATTTT‑aaa                                                                                                                          >  2:4292684/1‑140 (MQ=255)

                    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAAt                                                                                                                       <  2:3796039/140‑1 (MQ=255)

                    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTAATAC‑AAATTTT‑aaaaaa                                                                                                                       >  2:6783892/1‑139 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGT‑TTTTTTTTTATTTTTTTTTTAATTAATAC‑AAATTTT‑TAAAATa                                                                                                                      >  1:6608529/1‑140 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGT‑TTTTGTTTTTTTTTTTTTGTTATTAATAC‑AAATTTT‑aaaaaaa                                                                                                                      >  1:5018311/1‑138 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTAATAAAAAA‑AAATTTa‑aaaaaa                                                                                                                       >  1:6796825/1‑139 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATTTTATTAATAA‑AAATTTT‑aaaaaaa                                                                                                                      >  1:3687933/1‑138 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTATATTTTATTTAATTACTAC‑AAAAATG‑aaaaaaa                                                                                                                      >  2:4269076/1‑138 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGGTGTTTTTTATGTAATTAAGAA‑AAATTTT‑TAAAATa                                                                                                                      >  1:4280769/1‑140 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTGTTATTTCTACAAAATTTT‑aaaaaa                                                                                                                       >  1:333486/1‑139 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTTTTGTTTTTTATTTAATTACTAA‑AAAATTT‑AAAAATa                                                                                                                      >  2:3105469/1‑140 (MQ=255)

                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATTTTATTAGTAA‑AAATTTT‑AAAAATaac                                                                                                                    >  2:774444/1‑138 (MQ=255)

                            cgtcTTGCTTATTCTGGGCAGAGCTGGCAAATTTTTAAATTTATACCGATTCAAAAAAATTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGaaa                                                                                                               <  1:6313899/137‑1 (MQ=255)

                               cTTGCTGAATCGGTTCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAAc                                                                                                            <  2:2677616/140‑1 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAACTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTGTTTTTGTTTTTTATGTAATTACTAC‑AAAGTTG‑ACAAATAACGGaacacc                                                                                                            >  2:4186419/1‑136 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAATGTCAAACAAAAACGCCGGGGAAACCGGGCGT‑GTGTTTGTGCTTGTTTATTTAATTACGAC‑AAATTTG‑TATAATACgggggagac                                                                                                            >  2:3575917/1‑134 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTCTTTTTTAGTTAATTTTAAC‑AAAATTT‑GAAAATAAGGGaaaaaa                                                                                                            >  1:5380618/1‑139 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTGTTATTTTTTATGTAATTTCAAC‑AAAATTT‑AAAAAAAAGGGGAAAAc                                                                                                            >  2:3842312/1‑140 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAAc                                                                                                            <  2:488257/140‑1 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTTTTGTTTTTTTTTTTTTTAATAA‑AAAATTT‑AAAAATAATGGaaaaaa                                                                                                            >  1:3062179/1‑139 (MQ=255)

                                ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATTTAAATAAAAA‑AAATTTG‑AAAAATAAGGGAAAAACa                                                                                                           >  2:4352921/1‑140 (MQ=255)

                                ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTGTGTGTTCTTTTTTATGTAATTAATAA‑CAATTTG‑AAAAATACGGGCAAAACa                                                                                                           >  2:4836986/1‑140 (MQ=255)

                                  gatGAATCTGTGAATATCTGGTAAATTTTTAAATTTATACCTTTTTTAAAAATTTTAAAAAAAAACTACTGTGAAACAGTTAGT‑TTTTTTGTTATTTTTTATTTTATTTATAT‑TAATTTT‑AAAAATATGTGATAAACATc                                                                                                         <  2:5487004/138‑1 (MQ=255)

                                  gCTGAATCTTTGAAGAGCGGGGAAATTTTTAAATTTATACCTATTGAAAAAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATc                                                                                                         <  2:1227786/140‑1 (MQ=255)

                                   cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGT‑TTTTTTTTTGTTTTTTATGTAATTACTAC‑AAAATTG‑TATCAGACGGGTAAAAAAttc                                                                                                        >  2:7008190/1‑138 (MQ=255)

                                           gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGCCGT‑GGTTTTTTTGTTTTTTATTTAAATTATAC‑AAATTTT‑TAAAATGAGGGAAAAACATCGCAtatgat                                                                                                >  2:4943025/1‑135 (MQ=255)

                                                 agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTTTAATTAAGAC‑AAATTTT‑AAAAAAAAGGGAAAAAAAAACCCATTACTTACaaa                                                                                          >  1:5865964/1‑140 (MQ=255)

                                                     gttaaaTTTTTAAATTTATACCTATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAatat                                                                                      <  2:5931772/137‑1 (MQ=255)

                                                     gcgAAATTTTTAAATTTATACCGATTGAAACAAATTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAatat                                                                                      <  2:4216463/138‑1 (MQ=255)

                                                      ggAAATTTTTAAATTTATAGCGATTGAAACAAAGTAAAACAAAAACTCCTGGGAAACCGGGCGT‑TTTTTTGTTATTTTTTATGTAATTAATAC‑AAATTTG‑TATAATACGGGAAAAACATCCCCTTTAAGTCCcaaagagt                                                                                     >  2:5309717/1‑136 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGACACACAGTAAAACAAAAACGCCTGGTAAACCGGGCGT‑GTGTGTGTTGTTATTTATTTAAATACTAC‑AAATTTG‑AAAAATACAGCACAAACATACCATTTACTGCCAAAAAAtt                                                                                     >  2:565176/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGT‑TGTTTTGTTTTTTTTTTTGTTATTTATAA‑AAAATTT‑TAAAAAAAGGGTAAAAAATACAAATTAGTTGCAAAAAAtt                                                                                     >  1:3295991/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTAGTTTTTTTGTAATTAATAC‑AAAATTG‑AAAAAAAAGGGAAAAACATACCAATTAAGTCCAAAataat                                                                                     >  2:3357092/1‑138 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTTTTATTAATTA‑AAAATTT‑TAAAATAAAGGAAAAAAATCACAGTTAATTGAAAAAAAtt                                                                                     >  1:5852033/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTTTTTTTTATTTAATTAAAAA‑AAAATTT‑AAAAAAAAGGGAAAAACAAACCAGTTAATTCAAAAAAAtt                                                                                     >  1:1413755/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTTTTATTAAAAA‑AAATTTG‑AAAAATAACGGAAAAACAAACCAGTTACTTGCAAAAAAtt                                                                                     >  1:3218671/1‑140 (MQ=255)

                                                       taaaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTCTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                                                    <  2:4039502/139‑1 (MQ=255)

                                                         aaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                                  <  2:673827/140‑1 (MQ=255)

                                                           tttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGTAAACCGGTCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                <  2:1670103/140‑1 (MQ=255)

                                                           tttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                <  2:5950550/140‑1 (MQ=255)

                                                           tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTGTGTTTTTTATGTAATTAATAA‑AAATTTG‑AAAAAAAAGGGAAAAAAATACCATATAAGTACAAAATATatgatg                                                                                >  2:6245831/1‑140 (MQ=255)

                                                            ttttAAATTTATACCCATTCAAACAACGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                               <  1:2339183/140‑1 (MQ=255)

                                                                      atcccGTTTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                     <  1:1612185/137‑1 (MQ=255)

                                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTTTTTTTTTTTTAATTAATAC‑AAAATTT‑AAAAATACAGGCAAAAAATACCATTTTCTTACAAAAAATTTTGTGGTAAATTTATGTTGGtt                                                               >  1:7532574/1‑139 (MQ=255)

                                                                                aaaCAAATTAAAACAAAAACGCCTGCGAAACCGGGCGT‑TTTTTTGTTATTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                           <  2:7155612/140‑1 (MQ=255)

                                                                                aaaCAAAGAAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                           <  1:2857404/140‑1 (MQ=255)

                                                                                          aaacaaacACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  1:6098666/140‑1 (MQ=255)

                                                                                          aaacaaacACGCCTGGGAAACCGGGCGA‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AACTTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  2:2966818/140‑1 (MQ=255)

                                                                                          aaaaaaaaACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  1:862439/140‑1 (MQ=255)

                                                                                          aaaCAACCACGCCTGCGACACCCGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTCC‑AACTTTG‑CAAAATACGGGACAAACATCCCATTTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  2:4547577/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  1:3001630/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  1:1232772/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  1:2418401/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  1:4173208/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  2:2297032/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  1:6458312/140‑1 (MQ=255)

                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTgg                                                <  1:1655437/140‑1 (MQ=255)

                                                                                           taCAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTTTTGTTTTTTATTTAATTACTAA‑AAATTTG‑AAAAATACGGGAAAAACATGCCATTTACTTAGAAAATATTTGGTTGTAATTTTTTGTAGGTTAAATTTGTAGTTTgg                                                >  2:7256067/2‑140 (MQ=255)

                                                                                           aacaaacaCGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGc                                               <  1:1061493/140‑1 (MQ=255)

                                                                                           aaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATGTAATTAATAC‑AAATTTG‑AAAAATAAGGGAAAAACAACCCATTTACTTCAAAAATATTTGAAGGTAATTTTATGTAGGTAAAAATTGTA‑TTTggg                                               >  2:1399777/1‑139 (MQ=255)

                                                                                           aaCAAAAAAGCCTGGGAAACCGGGCGT‑GTGTTTTGTCTTTTTTATTTTTTTAATAA‑AAAATTGTAAAAAAACGGACAAAAC‑TAGCAGTTAGTTACAAAATATTTGATTTTAATTTTTTGTAGGTTAGATTTTTA‑TTTgtc                                               >  2:1089973/1‑138 (MQ=255)

                                                                                             cAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCg                                             <  1:3796960/140‑1 (MQ=255)

                                                                                               aacaCGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGtt                                           <  1:588313/137‑1 (MQ=255)

                                                                                                aaaCGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATGTAATTAATAC‑AAATTTG‑AAAAATAAGGGCAAAACATGCCATTTAAGTGCCAAATAGTTGATGTTTCCTTTATGTGGGTCAAATTTGTC‑TTTGGGCGtgc                                          >  2:6085839/1‑138 (MQ=255)

                                                                                                aaaCGCCTGGGAAACCGGGCGT‑TTTTGTGTGTTTTTTTATGTAATTACTAC‑AAATTTG‑TACAAAAAGGGCAAAAAATCCCATGTAATTAGCAAATATATTATTGTAATTTGTTTTAGGTTAAATGTGTA‑TTTGGGCGttg                                          >  2:2796125/1‑139 (MQ=255)

                                                                                                  aCGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATGTTATTAATAC‑AAATTTG‑AAAAAAAAGGGGAAAAAATACCAATTACTTCCAAAAGATTTTATTGTTATTTTATTTAGGTAAAATTTGTA‑TTTGGGCGTTGag                                        >  2:6487407/1‑140 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTATTTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCagag                                      <  1:7687582/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑ACAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCagag                                      <  1:5164815/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCagag                                      <  1:6361356/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCagag                                      <  1:4872118/140‑1 (MQ=255)

                                                                                                    gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCagag                                      <  1:2675818/140‑1 (MQ=255)

                                                                                                         ggAAACCGGGCTT‑TTTTTTGTTATTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGt                                 <  1:4349548/140‑1 (MQ=255)

                                                                                                         ggAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGt                                 <  1:1572754/140‑1 (MQ=255)

                                                                                                              ccGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGaaaa                            <  1:5530620/140‑1 (MQ=255)

                                                                                                                   cGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  2:6608529/140‑1 (MQ=255)

                                                                                                                   cGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  1:6866507/140‑1 (MQ=255)

                                                                                                                   agT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTACAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  1:7064183/139‑1 (MQ=255)

                                                                                                                           ttGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCt                <  2:2512313/140‑1 (MQ=255)

                                                                                                                            tGTTATTGTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGATAAACATCCCATGTACTTCCAAAATATTAGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTg               <  2:7698904/140‑1 (MQ=255)

                                                                                                                               tattttttATTTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat            <  2:5852033/138‑1 (MQ=255)

                                                                                                                                 ttttttATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAatat          >  2:5942368/1‑140 (MQ=255)

                                                                                                                                   ttttATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATAttt        >  1:2749957/1‑140 (MQ=255)

                                                                                                                                       aTGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtat    <  1:1912869/140‑1 (MQ=255)

CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTA‑TTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAA  >  NZ_CP034943/2991109‑2991384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`