BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,991,262	G→T	21.2%	intergenic (+63/‑144)	gbsR → / → bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,262	0	G	T	21.2%	424.3 / 50.8	229	intergenic (+63/‑144)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base G (57/117); new base T (53/2); total (110/119)
Fisher's exact test for biased strand distribution p-value = 1.67e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

GATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAGGGGGAATT  >  NZ_CP034943/2991125‑2991400

 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTAATAC‑AAAATTG‑aaa                                                                                                                                           >  2:2418401/1‑140 (MQ=255)

 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTTATAC‑AATTTTT‑aaa                                                                                                                                           >  2:3177146/1‑140 (MQ=255)

 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTAATTAATAC‑AAATTTT‑aaa                                                                                                                                           >  2:4292684/1‑140 (MQ=255)

  ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATAC‑AAATTTT‑aaaa                                                                                                                                          >  2:5607991/1‑140 (MQ=255)

  ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTACGAC‑AAATTTT‑aaaa                                                                                                                                          >  2:7178601/1‑140 (MQ=255)

    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAAt                                                                                                                                        <  2:3796039/140‑1 (MQ=255)

    ggCTGAAACGGATTGCTGACTCGGTGACGCGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAAt                                                                                                                                        <  1:7756512/140‑1 (MQ=255)

     gCTGAAACGGCTTGCTGAATCGGTGCATAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTCTGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAAAAAATAC‑AAATTTG‑aaaaaaa                                                                                                                                       >  2:4501659/1‑138 (MQ=255)

     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTTTTTGTTTTTTTTTTTTTGTTATTAATAC‑AAATTTT‑aaaaaaa                                                                                                                                       >  1:5018311/1‑138 (MQ=255)

     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTTAATTCTAC‑AAAATTG‑aaaaaaa                                                                                                                                       >  1:5856696/1‑138 (MQ=255)

     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATATTTTATTTAATTACTAC‑AAAAATG‑aaaaaaa                                                                                                                                       >  2:4269076/1‑138 (MQ=255)

         aaaCGGCTTGCTGAATCGTTGCAGAGCGGGTAATTTTTTAAATTTATACCTATTCAAAAAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACggg                                                                                                                                   <  1:554218/140‑1 (MQ=255)

            cgtcTTGCTTATTCTGGGCAGAGCTGGCAAATTTTTAAATTTATACCGATTCAAAAAAATTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGaaa                                                                                                                                <  1:6313899/137‑1 (MQ=255)

            cGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGaaa                                                                                                                                <  1:5328512/140‑1 (MQ=255)

               cTTGCTGAATCGGTTCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAAc                                                                                                                             <  2:2677616/140‑1 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGAACTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTGTTTTTGTTTTTTATGTAATTACTAC‑AAAGTTG‑ACAAATAACGGaacacc                                                                                                                             >  2:4186419/1‑136 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAATGTCAAACAAAAACGCCGGGGAAACCGGGCGTGTGTTTGTGCTTGTTTATTTAATTACGAC‑AAATTTG‑TATAATACgggggagac                                                                                                                             >  2:3575917/1‑134 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTAGTTAATTTTAAC‑AAAATTT‑GAAAATAAGGGaaaaaa                                                                                                                             >  1:5380618/1‑139 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTATTTTTTATGTAATTTCAAC‑AAAATTT‑AAAAAAAAGGGGAAAAc                                                                                                                             >  2:3842312/1‑140 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTTTTTAATTTATAA‑CAATTTG‑AAAAATAAGGGaaaaaa                                                                                                                             >  1:3529419/1‑139 (MQ=255)

               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTTTTTTTTTAATAA‑AAAATTT‑AAAAATAATGGaaaaaa                                                                                                                             >  1:3062179/1‑139 (MQ=255)

                ttGCTTAATCGGTGCAGCGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAACACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACa                                                                                                                            <  1:6951133/140‑1 (MQ=255)

                ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAAATAAAAA‑AAATTTG‑AAAAATAAGGGAAAAACa                                                                                                                            >  2:4352921/1‑140 (MQ=255)

                ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTGTGTGTTCTTTTTTATGTAATTAATAA‑CAATTTG‑AAAAATACGGGCAAAACa                                                                                                                            >  2:4836986/1‑140 (MQ=255)

                  gatGAATCTGTGAATATCTGGTAAATTTTTAAATTTATACCTTTTTTAAAAATTTTAAAAAAAAACTACTGTGAAACAGTTAGTTTTTTTGTTATTTTTTATTTTATTTATAT‑TAATTTT‑AAAAATATGTGATAAACAT‑c                                                                                                                         <  2:5487004/138‑1 (MQ=255)

                       actggggCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCAtt                                                                                                                    <  1:5944118/134‑1 (MQ=255)

                             tcAGAGCGTTGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTc                                                                                                              <  2:7581381/139‑1 (MQ=255)

                               agagCGGGTAAATTTTTAAATTTATACCGATTGAAACAAATTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCa                                                                                                            <  2:2743553/140‑1 (MQ=255)

                                 agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAAGAC‑AAATTTT‑AAAAAAAAGGGAAAAAAAA‑ACCCATTACTTACaaa                                                                                                          >  1:5865964/1‑140 (MQ=255)

                                 accGGGGAAATTATTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTATTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGACAAACAT‑CCCATGTACTTCCaaa                                                                                                          <  2:6694239/138‑1 (MQ=255)

                                     ggtaaaTTTTTAAATTTATACCTATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAatat                                                                                                      <  2:5931809/137‑1 (MQ=255)

                                     gggACATTTATAAAGTGATACCGATTCACACAAAGTAAAACAAACACGCCGCGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAatat                                                                                                      <  2:4528374/140‑1 (MQ=255)

                                     gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTATTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAatat                                                                                                      <  2:1564782/140‑1 (MQ=255)

                                     gcgAAATTTTTAAATTTATACCGATTGAAACAAATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAatat                                                                                                      <  2:4216463/138‑1 (MQ=255)

                                      ggAAATTTTTAAATTTATCCCGAGTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTACTTACTAC‑CAAATTG‑AAAAATTCCGGTAAAACAT‑GCCAGTTACATACAAAAAAtt                                                                                                     >  2:5204667/1‑140 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGTTGTTTTGTTTTTTTTTTTGTTATTTATAA‑AAAATTT‑TAAAAAAAGGGTAAAAAAT‑ACAAATTAGTTGCAAAAAAtt                                                                                                     >  1:3295991/1‑140 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTACTTACTAA‑AAAATTT‑TAAAATAAGGGGAAAACAG‑CCCATTTACTTGCaaaaaggg                                                                                                     >  2:5944118/1‑136 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATAC‑AAATTTG‑TAAAATAAGGGAAAAAAAA‑ACCAATTAATTGCAAAATAtt                                                                                                     >  2:5759104/1‑140 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTAGTTTTTTTGTAATTAATAC‑AAAATTG‑AAAAAAAAGGGAAAAACAT‑ACCAATTAAGTCCAAAataat                                                                                                     >  2:3357092/1‑138 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTATTTATACAAAATTTT‑TAAATAATGGAAAAAAAAA‑CCCATTTA‑TTACAAAatagt                                                                                                     >  1:2337531/1‑138 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTTATTAAAAA‑AAATTTG‑AAAAATAACGGAAAAACAA‑ACCAGTTACTTGCAAAAAAtt                                                                                                     >  1:3218671/1‑140 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTTTTTTATTAATAA‑AAATTTT‑AAAAATAAGGGAAAAAAAA‑CACACTTAATTCAaaaaaaat                                                                                                     >  1:76108/1‑136 (MQ=255)

                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTGTTTTTTTTGTTATTTATAA‑AAATTTT‑TAAAATAGGGGGAAAAAAT‑ACCAGTTTATTACAAAAAAtt                                                                                                     >  1:4736132/1‑140 (MQ=255)

                                       taaaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTCTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATAttt                                                                                                    <  2:4039502/139‑1 (MQ=255)

                                            ttttAAATTTATCCCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATgg                                                                                               <  2:6240997/140‑1 (MQ=255)

                                                      atcccGTTTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                                     <  1:1612185/137‑1 (MQ=255)

                                                      ataCCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                                     <  1:2864170/140‑1 (MQ=255)

                                                      ataCCGATAGAAACAACGTAACACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                                     <  1:5446177/140‑1 (MQ=255)

                                                           taTTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                                <  1:3017986/139‑1 (MQ=255)

                                                           gATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                                <  2:6755857/140‑1 (MQ=255)

                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTACTAC‑AAATTTT‑AAAAATAAGGGAAAAACAT‑ACCATTTAAGTCCAACATATTTTTTGGTAATTTTTTGTAggga                                                                               >  2:6628009/1‑138 (MQ=255)

                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTAATTAATAC‑AAAATTT‑AAAAATACAGGCAAAAAAT‑ACCATTTTCTTACAAAAAATTTTGTGGTAAATTTATGTTGGtt                                                                               >  1:7532574/1‑139 (MQ=255)

                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGGTTTTTTGTTGTTTTTTATTTAATTAATAA‑AAATATG‑AAAAAGACGGGAAAAACAT‑AGAATTTAAGTGCGAAAAATTTTATGGTTATTTTATGTAAGTa                                                                               >  2:7711019/1‑140 (MQ=255)

                                                                aaaaaaACTAAAACAAAAACGCCTGGGAAACCGGGCGATTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                           <  1:6763571/140‑1 (MQ=255)

                                                                      aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTGTAATTACTAC‑AAATTTG‑AAAAAAAAGGGCAAAACAA‑CCCCATTACATGCAAAATATTTTTTTGTAATTTTATGTTGGTTAAAATTG‑ttt                                                                    >  2:2506238/1‑138 (MQ=255)

                                                                          aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTACTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAACAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTgg                                                                <  1:5091249/140‑1 (MQ=255)

                                                                          aaaaaaACACGCCTGGTAAACCGGTCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTgg                                                                <  1:4432889/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTgg                                                                <  1:953980/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTgg                                                                <  1:4292684/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTgg                                                                <  1:6458312/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTgg                                                                <  1:2418401/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTgg                                                                <  2:2877029/140‑1 (MQ=255)

                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTgg                                                                <  1:1232772/140‑1 (MQ=255)

                                                                           aacaaacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGc                                                               <  1:1061493/140‑1 (MQ=255)

                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTAATAC‑AAATTTG‑AAAAATAAGGGAAAAACAA‑CCCATTTACTTCAAAAATATTTGAAGGTAATTTTATGTAGGTAAAAATTG‑TATTTggg                                                               >  2:1399777/1‑139 (MQ=255)

                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAAATAATAC‑AAATATT‑AAAAATACCGGTCAAACATACCCATTT‑TTTACAAAAAATGTGTTGGTAATTTTATGTTGGTAAAATTTT‑TAttttgg                                                               >  2:7064183/1‑137 (MQ=255)

                                                                           aaCAAAAAAGCCTGGGAAACCGGGCGTGTGTTTTGTCTTTTTTATTTTTTTAATAA‑AAAATTGTAAAAAAACGGACAAAAC‑T‑AGCAGTTAGTTACAAAATATTTGATTTTAATTTTTTGTAGGTTAGATTTT‑TATTTgtc                                                               >  2:1089973/1‑138 (MQ=255)

                                                                             cAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCg                                                             <  1:3796960/140‑1 (MQ=255)

                                                                               aacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGtt                                                           <  1:588313/137‑1 (MQ=255)

                                                                               aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGtt                                                           <  1:7299403/140‑1 (MQ=255)

                                                                                aaaCGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTATTTAATTACTAC‑AAAATTT‑AAAAATAACGGAAAAACAA‑CCCAATTTCTTCCAAAACATTTGGTGGTAATTTTTTTTAGGGAAAATTTG‑TATTTGGTCGtta                                                          >  2:198775/1‑139 (MQ=255)

                                                                                aaaCGCCTGGGAAACCGGGCGTGTTTTTGTTGGTTTTTATTTAATTATAAC‑AAAATTG‑AAAAATACGGGAAAAAAAT‑CACATTTAAATACAAAAAAATTGGCGTTAAATTTATTTACTTAAAATTTGATATTTTGCGGtc                                                           >  2:6029328/1‑139 (MQ=255)

                                                                                  aCGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTTTGTAATTACTAA‑AAAATTG‑AAAAATAAGGGAAAAAAAT‑CCCATTTACTTCCAAAATATTTGATTGTAATTTTATGTATGTAAAATTTT‑TATTTTGCCGTTGag                                                        >  2:1389331/1‑140 (MQ=255)

                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCagag                                                      <  2:2051071/140‑1 (MQ=255)

                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCagag                                                      <  1:6361377/140‑1 (MQ=255)

                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCagag                                                      <  1:2675818/140‑1 (MQ=255)

                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCagag                                                      <  1:1667114/140‑1 (MQ=255)

                                                                                         ggAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGt                                                 <  1:418837/140‑1 (MQ=255)

                                                                                              ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGaaaa                                            <  1:2829568/140‑1 (MQ=255)

                                                                                                   cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                                       <  1:6866507/140‑1 (MQ=255)

                                                                                                   cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                                       <  2:6608217/140‑1 (MQ=255)

                                                                                                          ttGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCt                                <  2:493539/140‑1 (MQ=255)

                                                                                                           tGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTg                               <  2:7750002/140‑1 (MQ=255)

                                                                                                              tattttttATTTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                            <  2:5852033/138‑1 (MQ=255)

                                                                                                                ttttttATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAatat                          >  2:3092313/1‑140 (MQ=255)

                                                                                                                  ttttATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGTATAttt                        >  2:2291933/1‑140 (MQ=255)

                                                                                                                      aTGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtat                    <  1:1912869/140‑1 (MQ=255)

                                                                                                                        gTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATaa                  <  2:6946943/140‑1 (MQ=255)

                                                                                                                          aaTTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATAGTTGTTGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGGCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACt                >  2:4759253/1‑140 (MQ=255)

                                                                                                                           aTTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACtt               >  1:3705258/1‑140 (MQ=255)

                                                                                                                            ttACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt              >  1:1449588/1‑140 (MQ=255)

                                                                                                                            ttACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt              >  1:2485413/1‑140 (MQ=255)

                                                                                                                              actac‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTAACTTCCAAAATATATGATGGTAATTTTATGTAGTTAAAATTAG‑TATTTGGCCGTTCAGAGGATGTGAAAATCGGGCAGGCCTGAATCTATTTATAACtgttt            >  2:1840936/1‑140 (MQ=255)

                                                                                                                                 ac‑AAATTTG‑AAAAATACTGGAAAAACAT‑CACATTTACTTACAAAATATTTGATGGTAATTTTATTTAGGTAAAATTTG‑TATTTGGACTTTCATAGGAGGTGAAAAGCTGGAAGGCCTGAATATTTTTATATCTTTTTAgg         <  2:6266084/140‑1 (MQ=255)

                                                                                                                                 ac‑AAATTTG‑AAAAATACGGGAAAAACAT‑ACCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgg         <  2:5311121/140‑1 (MQ=255)

                                                                                                                                  c‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAggg        >  2:254132/1‑140 (MQ=255)

                                                                                                                                  c‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAggg        >  2:6319520/1‑140 (MQ=255)

                                                                                                                                    aaaTTTG‑AAAAATACGTGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg       <  2:5083829/140‑1 (MQ=255)

                                                                                                                                    aaaTTTG‑AAAAATACGGGAAAAACAT‑CCCATTTTCTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAgggg       <  2:1176603/140‑1 (MQ=255)

                                                                                                                                      aTTTG‑AAAAATACGGGAAAAACAC‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCTTTCAGAGGAGGTGAAAAGCGGGCAGGGCTGTTTA‑ATTTATCATTTTTTAGGGGGaa    >  1:3374151/1‑140 (MQ=255)

GATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTG‑AAAAATACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTG‑TATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTAGGGGGAATT  >  NZ_CP034943/2991125‑2991400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`