Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,201,029 C→A 8.9% Y216* (TAC→TAA EO946_RS16570 → spore germination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,201,0290CA 8.9% 958.1 / 18.2 378Y216* (TAC→TAAEO946_RS16570spore germination protein
Reads supporting (aligned to +/- strand):  ref base C (135/193);  new base A (48/2);  total (183/195)
Fisher's exact test for biased strand distribution p-value = 1.67e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 394 total aligned reads displayed.

TTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTT  >  NZ_CP034943/3200890‑3201167
                                                                                                                                           |                                                                                                                                          
tttttGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATac                                                                                                                                            <  2:2237204/140‑1 (MQ=255)
     gACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACAtt                                                                                                                                       <  1:4100995/140‑1 (MQ=255)
     gACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACAtt                                                                                                                                       <  1:3896879/140‑1 (MQ=255)
     gACATCAATAATTTGCGCCCTGTTTTAGGCGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACAtt                                                                                                                                       <  1:1442943/140‑1 (MQ=255)
           aataatTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTGTCCAACTCTTTTTTAGGAATTGAACTGATGCTGTTTATTCCTGAAAATATGAAGAAAAAGAAATACACAATCAGata                                                                                                                                 >  1:1179680/1‑140 (MQ=255)
                 ttGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGc                                                                                                                           <  1:3238852/140‑1 (MQ=255)
                    cgcCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATTGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTAAGATATGCGGCTGt                                                                                                                        >  2:6552822/1‑140 (MQ=255)
                       ccTGTTGTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCgg                                                                                                                     <  2:2460740/140‑1 (MQ=255)
                         tGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTATTCCTGAACATATTAATAAAAAGAAATACAAATTCATATATGCGGGTTTCGGCt                                                                                                                   >  2:6801782/1‑140 (MQ=255)
                              tAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTATGTATGGAAGTGATGCTGTTTCTTCCTGAAAATATGAACAAAAACAAATAAACAATCAGATATGCGGCTGTCGGCTTTCTg                                                                                                              >  2:7331707/1‑140 (MQ=255)
                              tAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCATTTTTAGGAATGGAAGTGATGCTTTTTCTTCATGAACATATGAAGAAAAATAAATACACAATCACATATGCGGCTGTCGGCTTTCTg                                                                                                              >  2:1615419/1‑140 (MQ=255)
                                   gAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATTCGGCTGTCGGCTTTCTGTTTcc                                                                                                         >  2:5817096/1‑140 (MQ=255)
                                   gAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGAAGCTGTTTCTTACTGAAACTATGAAGAAAAAGAAAGACACATTCATATATGCGGGGGTCGGCTTTCTGATTcc                                                                                                         >  2:6434096/1‑140 (MQ=255)
                                   gAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCACTTTTTTAGGCATGGAAGTGATTCTGTTTATTGCTGAACATATTAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGGTGTCTGAttgc                                                                                                         >  1:5486147/1‑138 (MQ=255)
                                     atgTCTTGGACCCATTGCTAAATATATTAATGTCGTTTAAATATATTTTTTATTAATTGAAGTGATGCTTTTTAATACTTAAAATATGAATAAAAATAAATAAACATTAAGAAATTAGGATGTAGGCTTTCTTATTACGa                                                                                                       >  2:4918315/3‑140 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTGCATGTGTTTTTTACTAATTTAATTGATGCTGTTTATTAAAGAAAATATTAATAAAAAGAAATAAACAATAAGATATTCGGATGTGGGCTTTCTGATTcaca                                                                                                       >  1:7032319/1‑137 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                       <  1:7442292/140‑1 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                       <  1:658121/140‑1 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                       <  1:863081/140‑1 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                       <  2:6310495/140‑1 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                       <  1:2774947/140‑1 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCAAATCTTTTTTTGGAATGGAAGGGAAGGTGTTTATTACTTAACATATTAAGAAAAAGAAAAACACAATCACATATTCGGCTGTGGGCTTTCTTAttaaga                                                                                                       >  1:3347529/1‑136 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTACATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                       <  1:6102820/140‑1 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTACATCTCATTTTTAGGAATTGAAATGATTCAGTTTCTTACTGAACATATGAAGAAAAAAAAATACAAATTAAGATATTAGGCTGTCGGCTTTCTGATTCCgc                                                                                                       >  1:1662287/1‑139 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTACATCTATTTTTTAGGAATGGAACTGATGCTGTTTATTCCTGAACAAATGAATAAAAAGAAAAAAACAATCAGATATTCGGCTGTAGGCTTTCTGATTGCGa                                                                                                       >  1:6904161/1‑140 (MQ=255)
                                     aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTGTACATCGCTTTTTTAGGAATGGAAGTGATGCTGTTTATTCCTGAACATATGAATAAAAAGAAATCAACATTCAGATATTCGGGTGTCGGCGTGATGATTACgc                                                                                                       >  1:4892355/1‑139 (MQ=255)
                                     aGGTATTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                       <  2:7058855/140‑1 (MQ=255)
                                         cTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGTAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGattat                                                                                                   <  2:11320/140‑1 (MQ=255)
                                             taCCCTTTGCTAACTCTCTTACTGTGGTTTCCATATATTTTTTAGGAATGTAAGTGATGCTGTTTCTTCCTGAAAATATTAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTAttttt                                                                                               <  2:4771797/139‑2 (MQ=255)
                                              aCCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTtat                                                                                              <  1:4375756/140‑1 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTTTGCATCTCTTTTTTATGAATTGAAGTTATGCTGTTTATTCCTGAACAAATGAAGAAAAATAAATAAACATTCACATATTCGGCTGTAGGCTGTCTGATTACGATTATTTtatataa                                                                                          >  1:6485276/1‑139 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTTTGCATCTCTTTTTTATGAAAGGAAGTGATGCTGTTTATTCCTGAAAATATGAAGAAAAAGAAATAAACAATAAGATATGCGGCTGTCGGGTTTCTGATTACGATTATTTTatatat                                                                                          >  1:2958760/1‑140 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATTGAAGTGATTCTGTTTGTTTCTTAACAAATGAAGAAAAATAAATAAAAATTAAGAAATTCGGCTGTAGGCTTTGTTATTGGGATTTTTTTatatat                                                                                          >  1:4015272/1‑140 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATTCTGTTTGTTACTGAACATATGAAGAAAAATAAAAAAACAATAAGATATGCGGCTGTCGGCGTTGTGATTACGATTATTTTatatat                                                                                          >  1:2815314/1‑140 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGTAAAAGAAATACACATTCATATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                                                                          >  2:5174109/1‑140 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAACTGATGCTTTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCACATATTCGGCTGTCGGCTTTCTGATTCCCATTATTTTatatat                                                                                          >  2:717830/1‑140 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTTTCCATCTATTTTTTAGGAATGGAACTGATTCTGTTTCTTACTGAAACAATGAAGAAAAATAAATAAACAATAAGATATTCGGCTGTCTGCTTTATGAATCCGATTAATTTataaat                                                                                          >  2:5466583/1‑140 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTTTACCTCGGTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCATGAACATATGAAGAAAAAGAAATACACATTGAGTTATGCGGCTGTGGGGTTTGTGTTTCCGATTATTTTatatat                                                                                          >  1:5035744/1‑140 (MQ=255)
                                                  aTTGCTAACTCTCTTACTGTCGTGTCCATCTCTTTTTTTTGAATTGAAGTGATGCTGTTTGTTACTGAAAATATTAAGAAAAAAAAATACAAAAAAATATATTCGGCTGTGGGCTTTGTGTTTCGGATTATTTTatatat                                                                                          >  1:983268/1‑140 (MQ=255)
                                                   ttGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                                                                         <  1:4558349/140‑1 (MQ=255)
                                                   ttGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                                                                         <  1:5984659/140‑1 (MQ=255)
                                                        aaCTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGAc                                                                                    <  2:3942717/140‑1 (MQ=255)
                                                          ctctctTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACat                                                                                  <  2:7597975/140‑1 (MQ=255)
                                                            ctctTACTGTCGTTTGCATCGCTTTTTTAGGAATGGAAGTGATGCTGTTTATTGCTGAACAAATGAAGAAAAAGAAATACACATTCAGATATTCGGCTGTCGGCTTTGTGATTCCGAGTATTTTATATATACTGACaaat                                                                                >  1:2658954/1‑140 (MQ=255)
                                                              ctTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGt                                                                              <  2:722554/140‑1 (MQ=255)
                                                              ctTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCGGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGt                                                                              >  2:5650146/1‑140 (MQ=255)
                                                              cgtACTGTCGTGTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGt                                                                              <  2:6606840/138‑1 (MQ=255)
                                                               ttACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAACAAAAAGAAATACACATTCAGATATGCGGCTGTAGGCTTTCTGATTCCGATTATTTTATATATACTGACatattta                                                                             >  2:7480730/1‑137 (MQ=255)
                                                                tACTGTCGTTTCCAACTCTTTTTTATGAATTGAAGTGATGCTGTTTCTTCCTGAACATATTAATAAAAAGAAAAACACATTCAGAAATGCGGCTGTCGGCTTTCTGATTACGAGTATTTTATATATACTGACatatttaa                                                                            >  1:2012298/1‑136 (MQ=255)
                                                                 aCTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCAt                                                                           <  1:5301212/140‑1 (MQ=255)
                                                                  cTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATc                                                                          <  1:1384554/140‑1 (MQ=255)
                                                                        tttCCATCTCTTTTTTAGGAATGGAAGTGATTCTGTTTCTTCCTTAACATATTAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATTTATACTTACATATGTCATCgttgtt                                                                    <  2:298653/140‑1 (MQ=255)
                                                                        tttCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgttgtt                                                                    <  1:6848128/140‑1 (MQ=255)
                                                                             aTCTCTTTTTTACGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCGTTCTGATTCCGATTATTTTATATATCCTTACATATGTCATCGTTGTTGGGGc                                                               >  2:1828454/1‑140 (MQ=255)
                                                                                  ttttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGa                                                          <  2:437045/140‑1 (MQ=255)
                                                                                   tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                         <  1:7268068/140‑1 (MQ=255)
                                                                                   tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                         <  1:5670112/140‑1 (MQ=255)
                                                                                      ttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGc                                                      <  2:6694603/140‑1 (MQ=255)
                                                                                            aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACAGATTCAGATATGCGGCTGTCGGGTTTCTGATTCGGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                >  1:7187663/1‑140 (MQ=255)
                                                                                            aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                >  2:7431302/1‑140 (MQ=255)
                                                                                            aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                >  2:1486196/1‑140 (MQ=255)
                                                                                            aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                <  1:7526042/140‑1 (MQ=255)
                                                                                                     aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                       <  2:2324149/140‑1 (MQ=255)
                                                                                                     aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                       >  1:1904396/1‑140 (MQ=255)
                                                                                                     aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                       >  1:7711259/1‑140 (MQ=255)
                                                                                                       gCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGc                                     >  2:4657384/1‑140 (MQ=255)
                                                                                                           tttCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGat                                 >  2:3562993/1‑140 (MQ=255)
                                                                                                           tttCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGat                                 >  2:6192071/1‑140 (MQ=255)
                                                                                                             tcttcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGatat                               >  2:4150031/1‑140 (MQ=255)
                                                                                                                tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                            <  1:1002285/140‑1 (MQ=255)
                                                                                                                tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                            >  2:3392659/1‑140 (MQ=255)
                                                                                                                tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                            <  2:4995436/140‑1 (MQ=255)
                                                                                                                tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                            <  2:5355955/140‑1 (MQ=255)
                                                                                                                tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                            <  2:2528298/140‑1 (MQ=255)
                                                                                                                tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                            <  2:2772123/140‑1 (MQ=255)
                                                                                                                 ccTGAACATATGAAGGAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                           >  2:3154484/1‑140 (MQ=255)
                                                                                                                 ccTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                           >  2:4859658/1‑140 (MQ=255)
                                                                                                                 ccTGAACATATGAAGAAAAAGAAATACACATTCAGATACGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                           <  2:2396595/140‑1 (MQ=255)
                                                                                                                     aaCATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACt                       >  1:1121763/1‑140 (MQ=255)
                                                                                                                     aaCATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGACTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACt                       >  1:2184878/1‑140 (MQ=255)
                                                                                                                      aCATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACta                      <  1:4777159/140‑1 (MQ=255)
                                                                                                                       cATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACtat                     <  2:3677728/140‑1 (MQ=255)
                                                                                                                       cATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACtat                     <  2:3283724/140‑1 (MQ=255)
                                                                                                                         tatGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTatat                   <  2:6853265/140‑1 (MQ=255)
                                                                                                                         tatGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTatat                   <  1:3500518/140‑1 (MQ=255)
                                                                                                                          atGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATGCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACAGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtc                  >  2:2595354/1‑140 (MQ=255)
                                                                                                                          aagaagaaAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtc                  >  2:2826716/3‑140 (MQ=255)
                                                                                                                           tGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtct                 <  2:193533/140‑1 (MQ=255)
                                                                                                                           tGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtct                 <  2:4323542/140‑1 (MQ=255)
                                                                                                                           tGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtct                 <  2:6904034/140‑1 (MQ=255)
                                                                                                                           tGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtct                 <  1:6358421/140‑1 (MQ=255)
                                                                                                                           tGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtct                 <  2:5519481/140‑1 (MQ=255)
                                                                                                                                   aagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCa         <  2:445636/140‑1 (MQ=255)
                                                                                                                                   aagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCa         <  2:2001230/140‑1 (MQ=255)
                                                                                                                                   aagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCa         <  2:4015272/140‑1 (MQ=255)
                                                                                                                                    agaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAg        <  2:592147/140‑1 (MQ=255)
                                                                                                                                     gaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGt       <  2:7006401/140‑1 (MQ=255)
                                                                                                                                     gaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGt       <  2:7089299/140‑1 (MQ=255)
                                                                                                                                          acacATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCttt  <  1:7082637/140‑1 (MQ=255)
                                                                                                                                          acacATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCttt  <  2:6229609/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTT  >  NZ_CP034943/3200890‑3201167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: