Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,201,042 G→T 9.6% A221S (GCG→TCG)  EO946_RS16570 → spore germination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,201,0420GT 9.6% 1022.4 / 23.1 406A221S (GCG→TCG) EO946_RS16570spore germination protein
Reads supporting (aligned to +/- strand):  ref base G (145/207);  new base T (50/3);  total (196/210)
Fisher's exact test for biased strand distribution p-value = 1.74e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

TTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACT  >  NZ_CP034943/3200907‑3201181
                                                                                                                                       |                                                                                                                                           
ttGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGc                                                                                                                                         <  2:1882181/140‑1 (MQ=255)
      catGTTTTCGGTGTATGTCGTGGACCCATTGCCAACTCTCTTCCTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTTTTTCTTCCTTAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGATGTCgt                                                                                                                                   <  2:2461443/138‑2 (MQ=255)
        tGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTATTCCTGAACATATTAATAAAAAGAAATACAAATTCATATATGCGGGTTTCGGCt                                                                                                                                 >  2:6801782/1‑140 (MQ=255)
             tAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTATGTATGGAAGTGATGCTGTTTCTTCCTGAAAATATGAACAAAAACAAATAAACAATCAGATATGCGGCTGTCGGCTTTCTg                                                                                                                            >  2:7331707/1‑140 (MQ=255)
             tAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTGTTTAGGAATGGAAGTTATGCTGTTTCTTACTGTACATATGAATAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTATg                                                                                                                            >  2:2066880/1‑140 (MQ=255)
                  gAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCCTCTATTTTTTATGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGCAAAAGAAATACACATTCATATATGCGGCTGGCGGCTTTATGATTcc                                                                                                                       >  2:1718056/1‑140 (MQ=255)
                  gAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTcc                                                                                                                       <  2:1541367/140‑1 (MQ=255)
                    aGGTCTTGGACCCATTGCTACCTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                     <  2:6675213/140‑1 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTTGGAATGGAAGTGATGCTGTTTGTTCCTGAACATATGAAGAAAAAGAAATAAACAATAAGATATTCGGCTGTGGGCTTTCTGATTCCGa                                                                                                                     >  1:2911296/1‑140 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTATGAATGGAACTGATGCTGTTTCTTCCTGAAAATATGAATAAAAATAAATACACAATCAGATATGCGGCTGTCGGCTTTCTGATTACGa                                                                                                                     >  1:5546547/1‑140 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAATAAATAAACAATCAGATATTAGGCTGTGGGGGTTCTGATTGCGa                                                                                                                     >  1:592147/1‑140 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                     <  1:7442292/140‑1 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCGTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTTATTCCGa                                                                                                                     <  1:2806785/140‑1 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCGTTTTTATGAATGGAATTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTGTGATTCCGa                                                                                                                     >  1:4821465/1‑140 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCATTTTTAGGAATGGAAGTGATTCTGTTTGTTCCGGAACATATGAATAAAAAGAAAAAAACAATCAGATATGCGGCTGTCGGCGTTATGATTACGa                                                                                                                     >  1:1967665/1‑140 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCAGCTCTTTTTTAGGAATGGAAGTGATTCTGTTTCTTGCTGAACATATGAAGAAAAAGAAATAAACATTCAGAAATGCGGCTGTCGGCGGTATGATTCCgt                                                                                                                     >  1:1411245/1‑139 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTGTCCATCTCTTTTTTAGGCATGGAAGTGATGCTGTTTCTTCCTGAACATAGGAAGAAAAAGAAATACACAATCAGATATGCGGCTGTCGGGTTTCTGATTCGGa                                                                                                                     >  2:626887/1‑140 (MQ=255)
                    aGGTCTTGGACCCATTGCTAACTCACGTACTGTCGTTTACATCTCATTTTTATGAATGGAAGTTATGCTGTTTATTACTGAAAAAATTAAGAAAAATAAATACAAATTCATATATGCGGCTGAGGGCGTTATAATTCGGa                                                                                                                     >  2:3378228/1‑140 (MQ=255)
                        cTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGattat                                                                                                                 <  2:6595176/140‑1 (MQ=255)
                         ttGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGc                                                                                                                                         >  2:7027168/1‑115 (MQ=255)
                         ttGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGc                                                                                                                                         <  1:6886463/115‑1 (MQ=255)
                         ttGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGattatt                                                                                                                <  2:1046769/140‑1 (MQ=255)
                         ttGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGattatt                                                                                                                <  2:3648925/140‑1 (MQ=255)
                            taCCCTTTGCTAACTCTCTTACTGTGGTTTCCATATATTTTTTAGGAATGTAAGTGATGCTGTTTCTTCCTGAAAATATTAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTAttttt                                                                                                             <  2:4771797/139‑2 (MQ=255)
                            gACCCTTTGCTAAGTCTCTTAGTGTCGTTTCAATATATTTTTTAGGAATTGAAGTTTTGATTTTTCTACCTTAAAATTTGAAGAAAAAGAAATACACATTAAGATATGCGGGTGTCGGCTTTCTTATTCCGATTATTTta                                                                                                             <  2:4993883/140‑1 (MQ=255)
                             aCCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTtat                                                                                                            <  1:7069283/140‑1 (MQ=255)
                             aCCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTtat                                                                                                            <  1:4375756/140‑1 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTGCATCTCTTTTTTATGAATTGAAGTTATGCTGTTTATTCCTGAACAAATGAAGAAAAATAAATAAACATTCACATATTCGGCTGTAGGCTGTCTGATTACGATTATTTtatataa                                                                                                        >  1:6485276/1‑139 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTCCATCTGTTTTTTAGGAATGGAAGTGATTCTTTTTCTTCCAGAAAAAATGAATTAAAATAAATAAACATTAAAATATGCGGCTGTCGGCTTTATTATTACGATTAATTTatatat                                                                                                        >  2:2553566/1‑140 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTCCATCTGTTTGTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAATAAAAAGAAATAAAAATTCATCAATTCGGCGGTCGGCGTTGTGATTGCGGTTATTTTatatat                                                                                                        >  1:841712/1‑140 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTTGGAATTGAAGTGATTCTGTTTCTTACTTAACAAATTAAGAAAAATAAATAAACATTAAGATATTCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                                                                                        >  1:3574686/1‑140 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAATAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                                                                                        >  2:7082637/1‑140 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGTAAAAGAAATACACATTCATATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                                                                                        >  2:5174109/1‑140 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                                                                                        >  2:3500518/1‑140 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTCCATCTATTTTTTAGGAATGGAACTGATTCTGTTTCTTACTGAAACAATGAAGAAAAATAAATAAACAATAAGATATTCGGCTGTCTGCTTTATGAATCCGATTAATTTataaat                                                                                                        >  2:5466583/1‑140 (MQ=255)
                                 aTTGCTAACTCTCTTACTGTCGTTTCCAACTCTTTTTTAGGAATGGAAGTGATGCAGTTTCTTCCTGAAAATATGAAGAAAAAGAAATAAAAATTCAGATATGAGGCTGTCGGCGTTCTGATTACGATTATTTtaaattt                                                                                                        >  2:7125954/1‑138 (MQ=255)
                                  ttGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                                                                                       <  2:2704000/140‑1 (MQ=255)
                                   tGCTAACTCTCTTACTGTCGTTTCCCTCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTGCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATcc                                                                                                      >  2:6358421/1‑140 (MQ=255)
                                    gCTAACTCTCTTACTTTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACTTTCAGATATTCTGATGTCGGCTTTCTGATTCCGATTATTTTatatatgtt                                                                                                     <  1:1373290/140‑4 (MQ=255)
                                       aaCTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGAc                                                                                                  <  2:3942717/140‑1 (MQ=255)
                                         ctctctTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACat                                                                                                <  2:7597975/140‑1 (MQ=255)
                                            tctTACTGTCGTTTCCATCTGTTTTTTAGGAATGGAAGTGATGCTGTTTATTCCTGAACATATGAAGAAAAATAAATAAACATTCAGATATGCGGCTGTCGGCTTTGTGATTGCGATTATTTTATATATCATGACATATg                                                                                             >  1:5105677/1‑140 (MQ=255)
                                             ctTACTGTCGTTTCAATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGt                                                                                            <  2:5841783/140‑1 (MQ=255)
                                              ttACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAACAAAAAGAAATACACATTCAGATATGCGGCTGTAGGCTTTCTGATTCCGATTATTTTATATATACTGACatattta                                                                                           >  2:7480730/1‑137 (MQ=255)
                                                 cTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATc                                                                                        <  1:336441/140‑1 (MQ=255)
                                                 cTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATc                                                                                        <  1:6990148/140‑1 (MQ=255)
                                                 cTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATc                                                                                        <  2:6947573/140‑1 (MQ=255)
                                                   gTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgt                                                                                      <  1:6117935/140‑1 (MQ=255)
                                                    tCGTTTGCAGCTCTTTTTTAGGCATGGAAGGGCTGCTGTTTCTTGCGGTACAGAGGACGAAAAAGAAATACACATTCAGATATGCGGCTGGCGGCTTTCTGGAGGCGGTTATGTTATATATGGTGACAGATGTCATCgtg                                                                                     >  2:4176955/1‑139 (MQ=255)
                                                    tCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTATTCCTGAACAAATGAAGAAAAAGAAATACAAATTCAGATATGCCGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATAAGTCATCggt                                                                                     >  2:2798497/1‑138 (MQ=255)
                                                      gTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                     <  1:4069073/106‑1 (MQ=255)
                                                      gTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACAATCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTAATTTATATATCCAGACATATCTCATCgttgt                                                                                   >  2:1982568/1‑140 (MQ=255)
                                                       tttCCATCTCTTTTTTCGGAATGGAAGTGATGATGTTTATTCCTTAACATATGAAGAAAAAGAAATACACATTCAGATTTTCGGATGTCGTCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgttgtt                                                                                  <  1:1901537/140‑1 (MQ=255)
                                                       tttCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgttgtt                                                                                  <  1:1558168/140‑1 (MQ=255)
                                                                  tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTTGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                                       <  2:436278/140‑1 (MQ=255)
                                                                  tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                                       <  1:7268068/140‑1 (MQ=255)
                                                                    tttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCg                                                                     <  1:2724507/140‑1 (MQ=255)
                                                                           aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAATAAATACACATTCAGATATGCGGCTTTGGGCTTTCTGATTCCTATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                              <  1:717830/140‑1 (MQ=255)
                                                                           aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                              >  1:3302355/1‑140 (MQ=255)
                                                                           aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                              >  2:7431302/1‑140 (MQ=255)
                                                                           aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                              <  1:7526042/140‑1 (MQ=255)
                                                                                    aTGCTGTTTCTTCCTGAACATATGAAGAAGAAGAACTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTCTGTTATATATCCTGACATAGGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGaaacc                                                     >  1:4677918/1‑138 (MQ=255)
                                                                                    aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                                     <  2:3042190/140‑1 (MQ=255)
                                                                                    aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                                     >  1:7711259/1‑140 (MQ=255)
                                                                                    aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                                     >  1:5355955/1‑140 (MQ=255)
                                                                                    aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                                     >  1:317766/1‑140 (MQ=255)
                                                                                    aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                                     <  2:2324149/140‑1 (MQ=255)
                                                                                      gCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGc                                                   >  2:4657384/1‑140 (MQ=255)
                                                                                            tcttcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGatat                                             >  1:2140745/1‑140 (MQ=255)
                                                                                               tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                                          <  1:1002285/140‑1 (MQ=255)
                                                                                               tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                                          <  2:5355955/140‑1 (MQ=255)
                                                                                                ccagAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                         <  2:5936977/137‑1 (MQ=255)
                                                                                                ccTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                         >  2:4859658/1‑140 (MQ=255)
                                                                                                ccTGAACATATGAAGAAAAAGAAAGACACAGTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                         <  2:2397786/140‑1 (MQ=255)
                                                                                                  tGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGAGTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGa                                       <  1:957931/140‑1 (MQ=255)
                                                                                                    aaCATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACt                                     >  1:1121763/1‑140 (MQ=255)
                                                                                                        tatGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTatat                                 <  1:4967959/140‑1 (MQ=255)
                                                                                                        tatGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTatat                                 <  2:6853265/140‑1 (MQ=255)
                                                                                                        tatGAAGAAAAAGAAATACACATTCAGATATGAGGCTGTCGGCTTTCTTATTCCGATTATTTTATATATCCTGACATATGTCATAGTTGTTTGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCAGAatatat                                 <  2:7219002/140‑1 (MQ=255)
                                                                                                          tGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtct                               <  1:3931523/140‑1 (MQ=255)
                                                                                                          tGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtct                               <  1:6358421/140‑1 (MQ=255)
                                                                                                           gaagaaAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtctc                              >  2:5651055/1‑140 (MQ=255)
                                                                                                                 aaagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTc                        >  1:6603523/1‑140 (MQ=255)
                                                                                                                  aagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCa                       <  2:445636/140‑1 (MQ=255)
                                                                                                                  aagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCa                       <  2:317766/140‑1 (MQ=255)
                                                                                                                  aagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCa                       <  2:4015272/140‑1 (MQ=255)
                                                                                                                    gaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGt                     <  2:2881687/140‑1 (MQ=255)
                                                                                                                    gaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTAGATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGt                     <  2:6202785/140‑1 (MQ=255)
                                                                                                                        tACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATGAGGTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGAGATGGCCGACTATATCTCTCTTTCAGTCCtt                 >  2:6444651/1‑140 (MQ=255)
                                                                                                                         acacATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCttt                <  2:5561055/140‑1 (MQ=255)
                                                                                                                               tCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTc          >  1:5669575/1‑140 (MQ=255)
                                                                                                                               tCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTc          >  2:1326895/1‑140 (MQ=255)
                                                                                                                                cAGATATGCGGCTGTGGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCa         <  1:447401/140‑1 (MQ=255)
                                                                                                                                cAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCa         <  2:4145573/140‑1 (MQ=255)
                                                                                                                                    tatGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATgg                                           >  2:6742499/1‑102 (MQ=255)
                                                                                                                                    tatGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATgg                                           <  1:6742499/102‑1 (MQ=255)
                                                                                                                                       gCGGGTGTCGGCTTTCTGAGTCCGATTATTTTATATATCCTGACATATGTCATCGTTGGTGGGGCTTTCACCGCTCCCGAGGTGAAAACCCTGATATGGCCGCCTAGATATCTCTATCAGGCCTGTGAGCTCCAAGGAca  >  2:4924320/1‑139 (MQ=255)
                                                                                                                                       gCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACt  >  1:2527148/1‑140 (MQ=255)
                                                                                                                                       gCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACt  >  2:3687375/1‑140 (MQ=255)
                                                                                                                                       gCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACt  >  1:1205339/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
TTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACT  >  NZ_CP034943/3200907‑3201181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: