BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,312	C→A	6.0%	L136F (TTG→TTT)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,312	0	C	A	6.0%	1413.6 / 22.8	505	L136F (TTG→TTT)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base C (326/133); new base A (3/43); total (329/176)
Fisher's exact test for biased strand distribution p-value = 2.70e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 511 total aligned reads displayed.

TCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTT  >  NZ_CP034943/3280173‑3280450

          aaCAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTACAATTTTCtaa                                                                                                                                  >  2:7095145/1‑140 (MQ=255)

             atctGTCCCACGAACACTAAATTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATAGAACAAGCGGAATTGAATGTAAAAGAAAGTCGGTCGGTTTATTCGTTTTCCAATTTTCtaatta                                                                                                                               <  1:6444616/137‑1 (MQ=255)

              gATGTCGCACGAACACTAACTTCTTCAGATTTTCCAACTGTGCTGTACAACAAGTACGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaattaa                                                                                                                              <  2:7509740/140‑1 (MQ=255)

               aTGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaattaat                                                                                                                             >  1:3806428/1‑140 (MQ=255)

                  tCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTgg                                                                                                                          >  2:6171544/1‑140 (MQ=255)

                   cGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGt                                                                                                                         <  2:3739490/140‑1 (MQ=255)

                       cGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTagag                                                                                                                     <  1:5358050/140‑1 (MQ=255)

                        gAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGc                                                                                                                    <  2:41598/140‑1 (MQ=255)

                         aaCACTAACTTCCTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGcc                                                                                                                   >  2:4283667/1‑140 (MQ=255)

                                aCTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCaaa                                                                                                            >  1:7089460/1‑140 (MQ=255)

                                      tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAac                                                                                                      >  1:6894617/1‑140 (MQ=255)

                                      tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGACACTCAAAATCAac                                                                                                      >  1:3990148/1‑140 (MQ=255)

                                          aTTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGa                                                                                                  >  2:3219889/1‑140 (MQ=255)

                                            tttCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGc                                                                                                >  1:5523988/1‑140 (MQ=255)

                                              tCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCt                                                                                              >  2:5863770/1‑140 (MQ=255)

                                                   cggtgCTGTACAACAAGTAAGCCCGGTCACCCTTACTCTAACCAGCGGAATTTAATGTAAAACAAAGTCGCTCGGTGTATTCGTTTTCCAATTTTCGAAATAATTGGTAGAGCCACTCAAACTCAACACGCGCCTTTTTg                                                                                         <  1:2268415/138‑1 (MQ=255)

                                                      tgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTaa                                                                                      >  2:2273861/1‑140 (MQ=255)

                                                         tGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaat                                                                                   <  1:5027554/140‑1 (MQ=255)

                                                           tACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaatga                                                                                 >  1:3652875/1‑140 (MQ=255)

                                                           tACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaatga                                                                                 >  1:6029871/1‑140 (MQ=255)

                                                              aacaaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaatgaatg                                                                              <  2:7253094/140‑1 (MQ=255)

                                                              aacaaGTAAGCCAGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCTGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaatgaatg                                                                              <  1:7285127/140‑1 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTagag                                                                                                                     <  1:2554818/99‑1 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTagag                                                                                                                     >  2:2554818/1‑99 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                            >  2:5940675/1‑140 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                            >  1:5898354/1‑140 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                            >  1:2173995/1‑140 (MQ=255)

                                                                caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                            >  1:2995320/1‑140 (MQ=255)

                                                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                         <  1:1312977/140‑1 (MQ=255)

                                                                   gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGACTTTTTGTAAAATGAATGTTGAt                                                                         >  2:5210066/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGATTGTTGATTa                                                                       >  2:6581151/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  2:3909288/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       <  2:73213/140‑1 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  2:2369842/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  2:7445026/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  1:154731/1‑140 (MQ=255)

                                                                     aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                       >  2:785348/1‑140 (MQ=255)

                                                                      aGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaa                                                                      <  1:1783317/140‑1 (MQ=255)

                                                                        cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                    >  1:3897437/1‑140 (MQ=255)

                                                                        cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                    >  2:6261373/1‑140 (MQ=255)

                                                                          cGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGa                                                                  <  2:4747136/140‑1 (MQ=255)

                                                                             tCACCCTTAATATAACAAGCGGACTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTGTTGTAAAATGAATGTTGATTAAGAGATaa                                                               >  2:5698161/1‑140 (MQ=255)

                                                                                        ataACAAGCGGAATTTAATGTAAAAGAAACTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                    <  2:1484511/140‑1 (MQ=255)

                                                                                        ataACAAGCCGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                    <  2:4457281/140‑1 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACCCGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 <  2:2991001/140‑1 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGATTTTTATCat                                                 >  2:6976019/1‑139 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                  >  1:2699334/1‑139 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 <  2:7611793/140‑1 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:5418270/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:5580351/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:4556499/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:4911280/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:4239331/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:3179989/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:2839975/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:6308291/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:1960998/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:1966440/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:1782589/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  2:1574318/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTGGTAAAATGAATGTTGATTAGAAGATAAGTGATGTTTATCAg                                                 >  1:3537648/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTGTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                 >  1:5809976/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTACAATTTTCTAATTAATTGTTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGATTTTTATCAg                                                 >  2:1026442/1‑140 (MQ=255)

                                                                                           aCAAGCGGAATTTAATGAAAAAGAAAGTCGGACGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACACAAAATCAACACGAGCCTAATTGTAAAATGAATGTTGATTAAATGATAAGTGATTTTTATCAg                                                 >  1:5609314/1‑140 (MQ=255)

                                                                                               tcttattttaatttaaAATAAAGTGGGTGGGTGTTTTCTTTTTCAATTTTTCTATTTATTTGGTAGAGCCAATCAAATTCAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             <  2:2105804/134‑1 (MQ=255)

                                                                                               tcgtaaTTTATTTTAAAATAAAGTCGGTCGTTGTATTCGTTTTCCAATTTTCTATTTAATTGGTAGAGCAACTAAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTTTTTAAAAGATAATTGATTTTTATCAGaaaa                                             <  2:124188/136‑1 (MQ=255)

                                                                                               gggtattttATTGTAAAATAATGTGGGTGGGTGTATTCGTTTTCCTATTTACTATTTATTTGGTAGAGCCACTCAAAATCAACACGAGCATTTTTGTAAAATTAATGTTGATTAAAACATAAGTTATTTTTATCAGaaaa                                             <  2:4588346/134‑1 (MQ=255)

                                                                                               gcgtattttAATGTAAAAGAAATTCGGTGGGTGTATTCGTTTTCAAATTTTATATTTATTTGGTAGAGCCACTAAAAATCAACACGCGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCACaaaa                                             <  2:3503065/134‑1 (MQ=255)

                                                                                               gCGTAATTTAATTTAAAATAAATTGGGTGGGTGTATTGTTTTTCAATTTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCTTTTTTGTAAATTTAATGTTTATTAAAAGATATGTGTTTTTTATCACaaaa                                             <  2:6880457/140‑1 (MQ=255)

                                                                                               gCGGAATTTATTGTAAAAGAAAGTGGGTCGGTGTTTTGGTTTTCCTATTTTCTAATTATTTGGTAGAGCAACTCAAAATCAACCCGAGCCTTTTTGTAAAATGATTGTTTATTAAAAGATATGTTATTTTTATCAGaaaa                                             <  2:3083836/140‑1 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             >  1:289031/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             >  1:6532168/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATGTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                             >  1:514908/1‑140 (MQ=255)

                                                                                               gCGGAATTTAATGTAAAACAAAGTCGGTGGGTGTATTCGTTTTCCAATTTTCTAATTATTTGGTAGAGCAACTAAAAATAAACACGACCCTTTTTGTAAAATGAATTTTGATTAAAACATAAGTGTTTTTTATCAGaaaa                                             <  2:5141755/140‑1 (MQ=255)

                                                                                               ccgcaaTTTAATGTAAAAGAAAGTCGGTCGTTTTATTCGTTTTCCAATTTTATAATTAATTGGTCGAGCACCTAAAAATCAACACGAGCCTTTTTTTAAAATGAATGTTGATTAAAACATAAGTGATTTTTATCATaaaa                                             <  2:4393878/136‑1 (MQ=255)

                                                                                                   aaTTTATTGTAAACTAAAGTGGGTCGGTGTATTGTTTTTCCAATTTTATAATTATTTGGTAGAGCAACAAAAAATCAACCCGCGCCTTTTTGTAAAATGAATGTTGATTAAAATATAAGTTATTTTTATCAGAAAACGAc                                         <  2:3806428/140‑1 (MQ=255)

                                                                                                    aTTTAATGTAAAATCAAGTCGGTCTGTGTATTCGTTTACCAATTTTCTAATTATTTGTTAGAGCCACTCAAAAGCAACACGCGCCTTTTTGTAAAATGACTGTTTATTAAAACATAAGTGATTTTTATCAGAAAACGAcc                                        <  2:6075151/140‑1 (MQ=255)

                                                                                                          tGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATAc                                  >  1:1177443/1‑140 (MQ=255)

                                                                                                             aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAAACAACCCGACCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                               <  2:3593964/140‑1 (MQ=255)

                                                                                                               aagaaagTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACt                             >  1:6962602/1‑140 (MQ=255)

                                                                                                                      tcggtcggTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaa                      >  2:5509345/1‑140 (MQ=255)

                                                                                                                      tcggtcggTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaa                      >  2:4108303/1‑140 (MQ=255)

                                                                                                                        ggtgggtTTATTCTTTTTCAAATTTTATAATTATTTGTTCGCGCCAAAAAAAATAAACACGAGCTTTTTTTTAAAATTATTTTTGATTAAAAGATATGTTTTTTTTATCACAAAACGACCCTATACCGACTGAAGaaaaa                    <  1:2338721/140‑1 (MQ=255)

                                                                                                                         gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa                   >  1:3362273/1‑140 (MQ=255)

                                                                                                                         gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAGTAAAAGATACGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa                   >  1:7393835/1‑140 (MQ=255)

                                                                                                                                ttttCTTTTTAAAATTTTATAATTATTTGTTAGAGCAACTCAAAATAAACACGAGATTTTTTTTAAAATGAATTTTTATAAAAATATATTTTTTTTTTATAAAAACACGACCCTATACCGACTGAAGAAAAAAGGCCaaa            <  1:5940675/138‑1 (MQ=255)

                                                                                                                                tattttttttCAAATTTTCTAATTAATTGGTAGCGCAACACAAAATCAACACGAGCCTTTTTTTAAAATGAATTTTGATTAAAAGATATGTTTTTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa            <  1:1662229/134‑1 (MQ=255)

                                                                                                                                tATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa            >  1:6208063/1‑140 (MQ=255)

                                                                                                                                   tCGTTTTCCAATTTTATAATTAATTGGTAGCGCAACTCAAAACCAACACGAGCATTTTTTTAAAATGAATGTTTATTAAAAGATATGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAgg         <  2:7682928/140‑1 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:1008399/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:4729007/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:6694391/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:752858/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:79487/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:4391033/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  2:6717420/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  2:641956/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg    >  1:5730087/1‑140 (MQ=255)

                                                                                                                                        ttCCAATTTTCTAATTAATTGGTAGAGACACTCAAAATCAACACCAGCCTTTATGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAAAGACCCTATACCGACTGAATAAAAAAGGCCTAAAGGTGTgg    >  2:5060326/1‑140 (MQ=255)

                                                                                                                                          ccAATTTTCTAATTAATTTGTATATACAATAAAAATAAACAATAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAggctgtctc  >  2:496189/1‑133 (MQ=255)

TCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTT  >  NZ_CP034943/3280173‑3280450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`