BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,341	C→A	8.0%	E127* (GAG→TAG)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,341	0	C	A	8.0%	1334.0 / 24.3	502	E127* (GAG→TAG)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base C (312/133); new base A (4/53); total (316/186)
Fisher's exact test for biased strand distribution p-value = 6.44e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 502 total aligned reads displayed.

TAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAA  >  NZ_CP034943/3280203‑3280479

  aCTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCaaa                                                                                                                                         >  1:7089460/1‑140 (MQ=255)

      cttcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAATGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCa                                                                                                                                     >  2:4196624/1‑140 (MQ=255)

      cttcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCa                                                                                                                                     >  2:3053629/1‑140 (MQ=255)

          aGATTTTCCATCTGTGCTGTAAAACAATTAACCACTGTCACCCTTAATATAACAAGCTGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTACAATTTTCTAATTAATTGGTAGAGACACTCAAAATCAacaa                                                                                                                                 >  2:5423433/1‑139 (MQ=255)

              tttCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGc                                                                                                                             >  1:2004362/1‑140 (MQ=255)

                tCCATATGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCTGAATTTAATGTAAAAGAAACTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTAAAAATCAACACGAGCCt                                                                                                                           >  2:612425/1‑140 (MQ=255)

                 ccATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCtt                                                                                                                          >  2:7171372/1‑140 (MQ=255)

                      tgtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAACGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGt                                                                                                                     <  2:7495580/140‑1 (MQ=255)

                      tgtgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGt                                                                                                                     <  2:2394645/140‑1 (MQ=255)

                      tgtgCTGTACAACAAGGAAGCCCGGTCACCCTTAATCTAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGt                                                                                                                     <  1:5925448/140‑1 (MQ=255)

                             tACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaatga                                                                                                              >  2:3717350/1‑140 (MQ=255)

                                aacaaGTAAGCCAGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCTGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaatgaatg                                                                                                           <  1:7285127/140‑1 (MQ=255)

                                  caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                         >  1:2798371/1‑140 (MQ=255)

                                  caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGtt                                                                                                         >  1:6551986/1‑140 (MQ=255)

                                  caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTAGTTTTCCAATTTTCTAATTAATTGGTCGAGGCACTAAAAATCAACACCAGACTTTTTGTAAAATGAATGtt                                                                                                         >  2:4452304/1‑140 (MQ=255)

                                    aGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGa                                                                                                       >  2:5505193/1‑140 (MQ=255)

                                     gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                      <  1:2467446/140‑1 (MQ=255)

                                     gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                      >  1:455887/1‑140 (MQ=255)

                                     gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                      <  1:1312977/140‑1 (MQ=255)

                                     gTAAGCCCGGTCACCATTAATATAACAAGCGTAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                      <  1:2285126/140‑1 (MQ=255)

                                       aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                    >  2:785348/1‑140 (MQ=255)

                                       aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                    >  2:3817097/1‑140 (MQ=255)

                                       aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                    >  1:7502403/1‑140 (MQ=255)

                                       aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                    >  2:112668/1‑140 (MQ=255)

                                       aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                    >  2:4738056/1‑140 (MQ=255)

                                       aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                    >  2:2816342/1‑140 (MQ=255)

                                          cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTataa                                                                                                 >  2:5290039/1‑140 (MQ=255)

                                          cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                 >  2:6406847/1‑140 (MQ=255)

                                              gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAtata                                                                                             >  1:2988234/1‑140 (MQ=255)

                                              gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                             >  1:357172/1‑140 (MQ=255)

                                               tCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATaa                                                                                            >  2:2929146/1‑140 (MQ=255)

                                                cACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAg                                                                                           <  1:7741153/140‑1 (MQ=255)

                                                       aaTATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGAtttt                                                                                    >  1:496655/1‑140 (MQ=255)

                                                       aaTATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCAAATTTTCTAATTAATTGGTAGAGCAAATCAAAATAAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGAtttt                                                                                    >  1:3961153/1‑140 (MQ=255)

                                                         tataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTa                                                                                  >  1:1854842/1‑140 (MQ=255)

                                                          ataACCCGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAAGCAACACGAGCCTTTTTGTAAAATGAATGTGGATTAAAAGATAAGTGATTTTTAt                                                                                 <  1:5031740/140‑1 (MQ=255)

                                                          ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                 <  1:3507066/140‑1 (MQ=255)

                                                          ataACAAGCGGAATTTAATGTAAAAGAAACTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                 <  2:1484511/140‑1 (MQ=255)

                                                            aaCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                                               >  1:5755007/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAATGATAAGTGATTTTTATCAg                                                                              >  1:6783503/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGATTTTTATCat                                                                              >  2:6976019/1‑139 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  2:6890240/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              <  2:7611793/140‑1 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  2:1782589/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  1:3179989/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  1:2637087/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  1:2264379/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  1:6308291/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  1:6954483/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  1:4135119/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTGGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  1:6024365/1‑140 (MQ=255)

                                                             aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATAAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                              >  2:6736145/1‑140 (MQ=255)

                                                                 tgtgattttaatttaaACTATTGTCGGTCGGTGTAGTGGTTTTCAATTTTTATATTTAATTGGTAGAGCCACTCAAACTCAACACGAGCCTTTTTGTAAAATTAATGTTTATTAAAAGATAAGTGATTTTTATAAGaaaa                                                                          <  2:4340100/134‑1 (MQ=255)

                                                                 gctgAATTTATTTTAAAAGAATGTGGGTGGGTTTTTTGTTTTTCAATTTTTCTTTTTAATTGGTATAGCCACTAAAAATCAACACGAGCCTTTTTTTAAAATGATTGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                          <  2:2334091/137‑1 (MQ=255)

                                                                 gctgAATTTAATTTAAAATACATTGGGTCGTTTTATTGGTTTTCCAATTTTCTAATTATTTGGTAGAGCCACTCAAAATCAACACGAGCTTTTTTGTAAATTTAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                          <  2:3954966/137‑1 (MQ=255)

                                                                 gctgAATTTAATTAAAACCAAAGTCGGTGGTTTTATTCTTTTTCAATTTTTATAATTAATTGGTAGAGCCACTAAAAATCAACACGACCCTTTTTGTAAAATTAATGTTTATTAAAATATAAGTGATTTTTATCAGaaaa                                                                          <  2:3445245/137‑1 (MQ=255)

                                                                 gcgcAATTTAAGGTAAACGAAAGTCTGAGGGTTTTTTCGTTTTCCATTTTTATATTTATTTGGTTGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAAATGATTGTTGATTAAACGATAAGGGTTTTTTATCAGaaaa                                                                          <  2:4127069/140‑1 (MQ=255)

                                                                 gagtattttaatttaaAATAAATTCGGTGTGTTTATTCGTTTTCCAATTTTATAATTAATTGTTAGAGCCACTAAAAATAAACACGAGCCTTTTTGTAAAATTAATGTTTATTAAAATATAAGTGATTTTTATCAGaaaa                                                                          <  2:5980259/134‑1 (MQ=255)

                                                                 gCGTAATTTAATGTAAAAGAAAGTCGGTCGTTGTATACTTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTAAAAATCACCACGAGCCTTTTTGTAAAATGAATTTTGATTAAACGATACGTGATTTTTATCATaaaa                                                                          <  2:4564474/140‑1 (MQ=255)

                                                                 gCGTAATTTAATGTAAAAGAAAGTCGGTCGGTTTATTCTTTTTCCAATTTTATAATTAATTGGTAGAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                          <  2:1266434/140‑1 (MQ=255)

                                                                 gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTTTATTCTTTTTCAATTTTTATAATTATTTGGTAGAGCCACACAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                          <  2:3203763/140‑1 (MQ=255)

                                                                 gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                          >  1:5575558/1‑140 (MQ=255)

                                                                 gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                          >  1:3866044/1‑140 (MQ=255)

                                                                 gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATGTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                          >  1:514908/1‑140 (MQ=255)

                                                                 gCGGAATTTAATCTATAAGACAGTCGGGCGGTGTAGTCGTTTTCCAATTTTCTAATTAATGGGTAGAGCCACTCAAACTCAAACCGAGCCTTTTTGTAAATTTATTGTTGATTAAAAGATAAGTTATTTTTTTCAGaaaa                                                                          <  2:5075968/140‑1 (MQ=255)

                                                                   ttaatttaatttAAAATAAATTGGGTGGGTTTTTTCTTTTTCCATTTTTCTAATTATTTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCATAAAACg                                                                        <  2:5032568/138‑1 (MQ=255)

                                                                     attttAATGTAAAACAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTATAGCCACAAAAAATCAACACGACCATTTTTTTAAAATGAATGTTGATTAAAAGATACGTGATTTTTATCAGAAAACGAc                                                                      <  2:5679884/138‑1 (MQ=255)

                                                                     aaTTTAATGTAAACCAAAGTGGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGACACTCAAAATCAACACGTGCCTTTTTGTAAAATTAATGTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGAc                                                                      <  2:7288493/140‑1 (MQ=255)

                                                                            tGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATAc                                                               >  1:1177443/1‑140 (MQ=255)

                                                                               aaaaGAAAGTCGGTCGGTTTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTAAAAATCAACACGAGCATTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                            <  2:7363663/140‑1 (MQ=255)

                                                                               aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAAACAACCCGACCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                            <  2:3593964/140‑1 (MQ=255)

                                                                                      agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                    <  2:2515431/93‑1 (MQ=255)

                                                                                           gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa                                                >  1:3362273/1‑140 (MQ=255)

                                                                                           gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAGTAAAAGATACGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa                                                >  1:7393835/1‑140 (MQ=255)

                                                                                                  tattttttttCAAATTTTCTAATTAATTGGTAGCGCAACACAAAATCAACACGAGCCTTTTTTTAAAATGAATTTTGATTAAAAGATATGTTTTTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                         <  1:1662229/134‑1 (MQ=255)

                                                                                                  tATTCGTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACCCGAGCCTTTTTTTAAAATGAATTTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                         <  2:5757416/140‑1 (MQ=255)

                                                                                                     tCGTTTTCCAATTTTATAATTAATTGGTAGCGCAACTCAAAACCAACACGAGCATTTTTTTAAAATGAATGTTTATTAAAAGATATGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAgg                                      <  2:7682928/140‑1 (MQ=255)

                                                                                                         tttCAAATTTTTTAATTATTTTTTATACCAACTAAAATTCAAAACGCGCCTTTTTTTAAAAGTATTTTTTATTAAACTTTAAGTTATTTTTATCTGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGtgtg                                  <  1:5414693/140‑1 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAATAAAAAAGGCCAAAATGTGTgg                                 >  2:6221714/1‑140 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTCTACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                 >  1:5853913/1‑140 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGTCCAAAAGGTGTgg                                 >  1:4790726/1‑140 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                 >  1:1008399/1‑140 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                 >  2:5561119/1‑140 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                 >  1:3687462/1‑140 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                 >  1:4391060/1‑140 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                 >  1:864745/1‑140 (MQ=255)

                                                                                                          ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                 >  1:6456880/1‑140 (MQ=255)

                                                                                                            ccAATTTTCTAATTAATTTGTATATACAATAAAAATAAACAATAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAggctgtctc                               >  2:496189/1‑133 (MQ=255)

                                                                                                                 tttCTAATTAATTGGTAGAGCCACACAAAATCAACACCAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAACAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgt                          <  2:20799/140‑1 (MQ=255)

                                                                                                                   gccaattacttGGTCGACCCACTCAAAATCAACACCAGCGTTTTTGTAAAATGAATGTTTATAAAAAGATAAGTGATTTTTATAAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgttg                        <  2:5864203/137‑1 (MQ=255)

                                                                                                                   acTAATAAATGGGTAGAGCCACACAAAATCACCCCGAGCCTTTTTGTAAAATCAATTTTGATTAAAAGATAAGTGATTTGTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgttg                        <  2:5156807/139‑1 (MQ=255)

                                                                                                                       ataatttGGTAGAGCCACTCAAAAACAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAACATACGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGctct                    <  1:5629981/135‑1 (MQ=255)

                                                                                                                         taattGTTAGAGCCAATAAAAAACAACACGAGCATTTTTGTAAAATTAATGTTGATTAAAATATAATTCTTTTTTATAAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCtcttc                  <  2:6644600/140‑1 (MQ=255)

                                                                                                                         taattGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCtcttc                  <  2:1643189/140‑1 (MQ=255)

                                                                                                                         taattGGTAGAGCCACTCAAAATAAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAATTATAAGTTTTTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCtcttc                  <  2:6533202/140‑1 (MQ=255)

                                                                                                                                 agcgCCACTCAAAAACAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa          <  2:2192844/137‑1 (MQ=255)

                                                                                                                                 agagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa          >  2:1898139/1‑140 (MQ=255)

                                                                                                                                 agacCCACAAAAAATCACCACCCGCATTTTTTTAAAATGAATGTTTATAAAAAGATAAGTTATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa          <  1:1538429/140‑1 (MQ=255)

                                                                                                                                        cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa   >  1:3041694/1‑140 (MQ=255)

                                                                                                                                        cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa   >  1:6184924/1‑140 (MQ=255)

TAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAA  >  NZ_CP034943/3280203‑3280479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`