Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494317,8870GT31.6% 157.0 / 15.5 101E248D (GAG→GAT) dacAD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (48/19);  new base T (0/31);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 1.10e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 106 total aligned reads displayed.

TTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGCAATCTACATACAG  >  NZ_CP034943/17748‑18023
                                                                                                                                           |                                                                                                                                        
ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                                          >  2:2091939/1‑140 (MQ=255)
ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                                          >  1:2627743/1‑140 (MQ=255)
ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                                          >  1:20903/1‑140 (MQ=255)
ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                                          >  2:2674544/1‑140 (MQ=255)
ttCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAg                                                                                                                                          >  1:1715045/1‑140 (MQ=255)
     gCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAACGGGCTTGTCAGCGAGTAGaa                                                                                                                                     >  1:2690595/1‑140 (MQ=255)
     gCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaa                                                                                                                                     >  1:649176/1‑140 (MQ=255)
     gCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaa                                                                                                                                     >  2:1837525/1‑140 (MQ=255)
     gCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATaa                                                                                                                                     >  1:2073170/1‑140 (MQ=255)
                   tACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAg                                                                                                                       >  1:2582419/1‑140 (MQ=255)
                     cTGACTACCCTTATATTCTGCAGACTTCCTGTATGTCTAAAACGAAGTTCCTAGATGGCACAGATGATGAAATGGACATGCCGAAGTGCAATTTTTTGCTGAATGGGGTTGTCAGCGTTTATAAAAAAGCGACTGTAGAc                                                                                                                     <  2:2534786/140‑1 (MQ=255)
                        aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTCTAAAAAAGCGACTGTAGACGGa                                                                                                                  >  2:578176/1‑140 (MQ=255)
                        aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                                                  >  1:77554/1‑140 (MQ=255)
                        aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                                                  >  1:1297388/1‑140 (MQ=255)
                        aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGa                                                                                                                  >  2:1085377/1‑140 (MQ=255)
                        aCTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGAAGACGGa                                                                                                                  >  2:1248752/1‑140 (MQ=255)
                            cccTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGa                                                                                                              >  2:1545891/1‑140 (MQ=255)
                            cccTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGa                                                                                                              >  2:2319626/1‑140 (MQ=255)
                               tGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGaaaa                                                                                                           >  1:39518/1‑140 (MQ=255)
                                gatcttgtGGAGACTTCCATTATCGATAAACCCAAGTTCAGAGAAGGCACACATTATTAATTGGACATGCCGAACTTCAACTTTATGCTGAACGGGCTTGTCAGCGAGTAAAAAAAAGCGACTGTAGACGGATTGAAAAc                                                                                                          <  2:1896192/133‑1 (MQ=255)
                                      cTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGCTGATGAAATGGACATGCCGAACTGGAACTTTATGTTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTc                                                                                                    >  1:152782/1‑140 (MQ=255)
                                      cTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTc                                                                                                    >  1:270700/1‑140 (MQ=255)
                                      cTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTc                                                                                                    >  1:779279/1‑140 (MQ=255)
                                       tGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGACGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCt                                                                                                   >  2:1510492/1‑140 (MQ=255)
                                       tGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTCTAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCt                                                                                                   >  1:584490/1‑140 (MQ=255)
                                       tGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCt                                                                                                   >  2:1864534/1‑140 (MQ=255)
                                        ggAGAGTTCCAGTATGGCTAAACCGAAGTTCAGAGACGGCACAGATGATGAACGGGACAGGCCGAACTGCAACTTTATGCTCAACGGGCTTGACAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTa                                                                                                  <  2:2157994/140‑1 (MQ=255)
                                              ttCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGAct                                                                                            >  1:1332898/1‑140 (MQ=255)
                                                 cattATCGATAAAACGACTTTCAGATACGGCACAGTTTATTAACTGGACATCCCTAACTGGAACTTTTTGCTCAAAGGGCTTGTCCGCTATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTg                                                                                         <  2:1339571/137‑1 (MQ=255)
                                                 aattATCCCTAAACCCACGTCCCGAGACGCCACACAGCATTAAATGCACATGCCGAACTGCAACTTTTGGCTCAACGGGTTTGCCCGCGATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTg                                                                                         <  2:77554/137‑1 (MQ=255)
                                                      tCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGc                                                                                    >  1:325265/1‑140 (MQ=255)
                                                        gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTCGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                                                  >  1:1595358/1‑140 (MQ=255)
                                                        gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                                                  >  1:273091/1‑140 (MQ=255)
                                                        gCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTc                                                                                  >  1:1416179/1‑140 (MQ=255)
                                                         caaaaaCGAAGTTCAGATCAGGAACATATGATCAACTGGAACTCCCTACCTGGAAATTTATGCTCAAAGGGCTGGTAAGCCATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCa                                                                                 <  2:330165/138‑1 (MQ=255)
                                                         cTAACACTAAGTTCAGAGAAGGCACAGATTATGAAATGCACTTCCCTAAGTGTAACTTTTTGCTGAAAGGGCTTTTCTGCTAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCa                                                                                 <  2:791790/140‑1 (MQ=255)
                                                         cTAAACCGACTTTCAGATAAGGCAACGATTATGAAATTCACTTGCCGAAATTCAACTTTTTGCTGAACGGGCTTGTCAGCTAGTATAAAAAAGCGACTGTAGACTGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCa                                                                                 <  2:1103951/140‑1 (MQ=255)
                                                         cTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCa                                                                                 >  2:1721061/1‑140 (MQ=255)
                                                         cTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCa                                                                                 >  1:2131525/1‑140 (MQ=255)
                                                         cTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCa                                                                                 >  1:437420/1‑140 (MQ=255)
                                                            aacgcacgTTCAGAGAAGGAACACATCTTGAAAGGCACTTCCCGACCTGCAACTTTATGCTCAACGGGGGTGTCAGCGTGTATAAAAAAGCGACTGTAGTCGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                              <  2:2560411/133‑1 (MQ=255)
                                                            aaaCGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                              >  1:2509084/1‑140 (MQ=255)
                                                            aaaCCAAGTCAAGACACGGCACCGATCATCAACTGGACATGCCTAAGTGTACCTTTATGGTTAAAGGGCTTGTCAGCTATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGc                                                                              <  2:536617/140‑1 (MQ=255)
                                                              aCGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCtt                                                                            >  1:667359/1‑140 (MQ=255)
                                                                  aGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACa                                                                        >  2:970533/1‑140 (MQ=255)
                                                                  aGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACa                                                                        >  2:487770/1‑140 (MQ=255)
                                                                  aGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACa                                                                        >  2:577446/1‑140 (MQ=255)
                                                                    ttcctagaAGCCACAGTTCATGACATGGACACGCCGAACTGGAAATTTATGCTGAACGGGCTTGACTGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAgg                                                                      <  2:1718161/135‑1 (MQ=255)
                                                                    tacctaaaaGGAAAAGATGATTAATTGGAACGGCCGACGGGGAAGTTTATGCTTAATGGGTTTGTCAGCTAGTAAAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAgg                                                                      <  1:2134451/133‑1 (MQ=255)
                                                                         atatgcaacaTATTATTAATTGCACTTGCCTAACGGTAATTTTATGATTAACGGGTTTTTCAGGCATTATAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAg                                                                 <  1:1992367/133‑1 (MQ=255)
                                                                         agaAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAg                                                                 >  2:2626142/1‑140 (MQ=255)
                                                                         acacggCACCGATCAGAAAAGGCACATCCCGACCTGCAAATTTATGCTGAACGGGTTTGTCAGCGTTTAAAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAg                                                                 <  1:1992794/136‑1 (MQ=255)
                                                                           aaGGCACAGAGGTTCAACTGCACATGGCTAACTGGAACTTTTTGCGGAACGGGGTGGTCCGCGATAAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCt                                                               <  2:2337471/140‑1 (MQ=255)
                                                                           aaGCCACATATGATTAATTGTACATGCGTAACTGGAAATTTTTGCTCAATGGGGTTGTAAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCt                                                               <  2:2165658/140‑1 (MQ=255)
                                                                              gCACAGATTATTAAATGGACATGCCGAACTGTAACTTTATGCTGAACGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGaa                                                            <  2:1656730/140‑1 (MQ=255)
                                                                              gCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGaa                                                            >  1:223955/1‑140 (MQ=255)
                                                                              gCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGaa                                                            >  1:1301371/1‑140 (MQ=255)
                                                                                   gatgatGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCaa                                                       >  1:1034031/1‑140 (MQ=255)
                                                                                       tttaaaTCGACATGCCGAACTGTAACTTTATGCTGAACGGGCTTGTCAGCGATAATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGa                                                   <  2:1755385/137‑1 (MQ=255)
                                                                                       atcaaaTGGCCATGCCGAACTGCAAGTTTATGCTGAACGGGGTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGa                                                   <  2:2567082/137‑1 (MQ=255)
                                                                                        tgaAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGACCAGCTGAACTCAACGGaa                                                  >  1:2567082/1‑140 (MQ=255)
                                                                                          aatgggACATGCCGAACTGCACGTTTAGGCTGAACGGGTTTGTCAGCGAGTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                                <  2:223955/136‑1 (MQ=255)
                                                                                          aactGGACATGCCCAAGTGTAAGTTTTTGCTGAACGGGCTTGTCCGCGATTATAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                                <  1:2319626/137‑1 (MQ=255)
                                                                                          aaaggcaCATGCCCAACGGTAAATTTTGGCTCAATGGGCTTGTCAGCTATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                                <  1:1150946/134‑1 (MQ=255)
                                                                                          aaaTTGACATGCCTAACGGGAACTTTATGGTGCATGGGTTTGTCAGCGACAAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGGTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                                <  1:2667169/140‑1 (MQ=255)
                                                                                          aaaTGGACATGCCGAACTGGAAATTTATGCTGAAAGGGGTTGTCAGGGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                                <  2:1856190/140‑1 (MQ=255)
                                                                                          aaaTGGAATTGCCTAAGTGTATCTTTTTGCTGAATGGGGTTGTCCTGTATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                                <  2:2495522/140‑1 (MQ=255)
                                                                                          aaaGGGACATGCCGAAGTGGAACTTTATGCTGAATGGGCTTGTCAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATg                                                <  2:2131525/140‑1 (MQ=255)
                                                                                            ttgtaattGCCTAACTGTAATTTTTTGCTTAACGGGTTTGCCAGCGCTTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGGTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                              <  2:325265/133‑1 (MQ=255)
                                                                                            ctggccctGCCCACCTGCAACTTTATGCTCAACGGGCTTGTCAGCTCCAATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCg                                              <  1:2148100/133‑1 (MQ=255)
                                                                                              ggACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtg                                            >  2:571500/1‑140 (MQ=255)
                                                                                              ggACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtg                                            >  1:1755385/1‑140 (MQ=255)
                                                                                               taCATGCCTAAGTGTAAGTTTATGCTGAATGGGTTTGTCAGCGTTTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCgtgt                                           <  2:1562443/139‑1 (MQ=255)
                                                                                                  aTGCCTAACTTTAATTTTATGCTTAAAGGGTTTGTCTGCTATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttat                                        <  2:2627743/140‑1 (MQ=255)
                                                                                                    gCCTAAGTGTAACTTTAGGCTTACTGGGCTTGCCCGCTATAATAAAACAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta                                      <  1:1634263/140‑1 (MQ=255)
                                                                                                    gCCTAACTTGAACTTTATGCTTAAAGGTTTTGTCAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta                                      <  2:653855/140‑1 (MQ=255)
                                                                                                    gCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta                                      >  1:2495522/1‑140 (MQ=255)
                                                                                                    gCCGAACTGGAACTTAATGCTGAACGGTCTTGAACGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta                                      <  2:506610/140‑1 (MQ=255)
                                                                                                    gCCGAACTGCAATTTTATGCTGAACGGGGTGGTCAGCGATAAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta                                      <  1:100887/140‑1 (MQ=255)
                                                                                                    ccccaccTGGAAGTTTTTGCTCAACGGGCTTGTCAGCGAGTAAAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGttatta                                      <  1:1458667/134‑1 (MQ=255)
                                                                                                       gAACTGGAACTTTATGCTGACCGGGCTGGACAGCGCCTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACtg                                   <  1:1207543/140‑1 (MQ=255)
                                                                                                       ccaCTGGAACTTTATGCTGAACGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACtg                                   <  2:1485912/138‑1 (MQ=255)
                                                                                                         aCTGGACCTTTTTGCTTACAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACtgtt                                 <  2:437420/140‑1 (MQ=255)
                                                                                                               aCTTTATGCTGAAAGGGCTTGTCAGCGAGGATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTGCTACTTACTCTGCGGGCTCAGGCTTTACAGGACCAGCTGAACTCAACGGAATGCGTGTGATTACTGTTGTATTa                           >  1:988693/1‑140 (MQ=255)
                                                                                                                cTTTATGCTGACCGGGCTTGTCCGCTCGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTaa                          <  2:52313/140‑1 (MQ=255)
                                                                                                                   tATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACg                       >  2:1356321/1‑140 (MQ=255)
                                                                                                                   tATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACg                       >  1:455199/1‑140 (MQ=255)
                                                                                                                     tGCTTAATGGGTTTGTCAGCGATTAAAAAAACGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCa                     <  1:2590254/140‑1 (MQ=255)
                                                                                                                          aaaGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAgg                >  2:597622/1‑140 (MQ=255)
                                                                                                                           gaGGGTTTTTCAGCTTGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc               <  2:63255/139‑1 (MQ=255)
                                                                                                                           atgtggttGTAAGCGTTTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc               <  2:2582419/134‑1 (MQ=255)
                                                                                                                           atggggTTGTCAGCGATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc               <  1:1191034/138‑1 (MQ=255)
                                                                                                                           atgggCTTGTCAGCTATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc               <  1:1400810/138‑1 (MQ=255)
                                                                                                                           acggggTTGTCCGCTATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc               <  2:211547/138‑1 (MQ=255)
                                                                                                                           acgggCTTTTCAGCGATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc               <  2:1301371/138‑1 (MQ=255)
                                                                                                                           acgggCTTGTCAGCTATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc               <  2:1715045/138‑1 (MQ=255)
                                                                                                                           aaGGGATTGTCAGCGTTTATAAAAAAGCGACTGTAGTCGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGc               <  1:1143837/140‑1 (MQ=255)
                                                                                                                               ggttGTCACCGATTAAAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGCAATc           <  2:201968/138‑1 (MQ=255)
                                                                                                                                  tGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGCAATCtac        >  2:2146401/1‑140 (MQ=255)
                                                                                                                                     cAGCTATTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGCAATCtacata     <  1:2201166/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TTCTTGCGGATCACTTGATTACTGACTACCCTGAGATTCTGGAGACTTCCAGTATCGCTAAAACGAAGTTCAGAGAAGGCACAGATGATGAAATGGACATGCCGAACTGGAACTTTATGCTGAAAGGGCTTGTCAGCGAGTATAAAAAAGCGACTGTAGACGGATTGAAAACAGGTTCTACTGACTCTGCGGGCTCATGCTTTACAGGAACAGCTGAACTCAACGGAATGCGTGTTATTACTGTTGTATTAAACGCAAAAGGCAATCTACATACAG  >  NZ_CP034943/17748‑18023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑