| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 2,981,253 | 0 | C | T | 46.7% | ‑6.0 / 18.4 | 15 | noncoding (1267/1550 nt) | EO946_RS15435 | 16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (0/8); new base T (2/5); total (2/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG > NZ_CP034943/2981117‑2981355 | tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTAAACCTCGCGGTTTCGCt < 1:3044733/137‑1 (MQ=1)tgaaCAAGGCCCGGGAACGTATTCAAAGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCt > 2:3164302/4‑140 (MQ=0) cctggAACTTATTCACCGCGGCCTGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGtt < 1:2090034/137‑1 (MQ=0) cctggAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGtt < 2:945004/137‑1 (MQ=1) taatCACCGCGGCATGCTGATCAGCTATTAAAATCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCAtt > 2:1506017/4‑140 (MQ=1) gtccccGCGGCATGCAGATCCGCGAGTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGt < 1:3246228/136‑1 (MQ=0) acgcgtgaTGCTGCTCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGATTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCa < 2:304078/133‑1 (MQ=0) caggCTGATCCGCGATTACTCGCGATTCCAGCTTCCCGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCATTGTAGCACgtgtg < 1:2265630/137‑1 (MQ=0) caagCTGATCCGCGATTACGAGCGATTCCAGCTTCACGCACTCGACTTGCAGACTGCGCTCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACgtgtg < 1:643587/137‑1 (MQ=1) gagccGCGATTACTCGCGAATCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGccc < 2:2189471/137‑1 (MQ=0) gagtCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACTTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCatg < 1:1651926/137‑1 (MQ=1) gagtCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGAGTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCatg < 2:2652518/137‑1 (MQ=1) caagCAGTCGAGTTGCAGAATGCTATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCACTTTGTTCTGTCGATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATTATTTGACg < 1:2792465/137‑1 (MQ=1) cgaccAGACTTGCGGGATTGGCTAAACCTCGCGGTTTCGCGGCCCTTTGTTCTGTCCATTGGAGCACGTGTGTAGCCCAGGTCATACGGGGCATGATGATTTGACGTCATCCCCACCGTCCTCCGGTTTGTCACCGGCAg < 2:2979382/136‑1 (MQ=1) agcaCAGATTTGTGGGAGTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAg < 1:1052160/137‑1 (MQ=0) | TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAG > NZ_CP034943/2981117‑2981355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |