mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | PAL128_S126_L001_R1_001.good.fq | 3,869,402 | 525,293,148 | 100.0% | 135.8 bases | 140 bases | 98.8% |
| errors | PAL128_S126_L001_R2_001.good.fq | 3,869,402 | 525,293,148 | 100.0% | 135.8 bases | 140 bases | 97.9% |
| total | 7,738,804 | 1,050,586,296 | 100.0% | 135.8 bases | 140 bases | 98.4% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NZ_CP034943 | 4,045,538 | 251.4 | 11.9 | 100.0% | Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499 strain |
| total | 4,045,538 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 4064 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 167 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.012 |
| reference sequence | pr(no read start) |
|---|---|
| NZ_CP034943 | 0.70954 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 11:01:42 16 May 2023 | 11:03:55 16 May 2023 | 2 minutes 13 seconds |
| Read alignment to reference genome | 11:03:55 16 May 2023 | 11:20:19 16 May 2023 | 16 minutes 24 seconds |
| Preprocessing alignments for candidate junction identification | 11:20:19 16 May 2023 | 11:22:12 16 May 2023 | 1 minute 53 seconds |
| Preliminary analysis of coverage distribution | 11:22:12 16 May 2023 | 11:26:10 16 May 2023 | 3 minutes 58 seconds |
| Identifying junction candidates | 11:26:10 16 May 2023 | 11:26:12 16 May 2023 | 2 seconds |
| Re-alignment to junction candidates | 11:26:12 16 May 2023 | 11:30:25 16 May 2023 | 4 minutes 13 seconds |
| Resolving best read alignments | 11:30:25 16 May 2023 | 11:33:10 16 May 2023 | 2 minutes 45 seconds |
| Creating BAM files | 11:33:10 16 May 2023 | 11:36:33 16 May 2023 | 3 minutes 23 seconds |
| Tabulating error counts | 11:36:33 16 May 2023 | 11:38:16 16 May 2023 | 1 minute 43 seconds |
| Re-calibrating base error rates | 11:38:16 16 May 2023 | 11:38:17 16 May 2023 | 1 second |
| Examining read alignment evidence | 11:38:17 16 May 2023 | 11:57:17 16 May 2023 | 19 minutes 0 seconds |
| Polymorphism statistics | 11:57:17 16 May 2023 | 11:57:18 16 May 2023 | 1 second |
| Output | 11:57:18 16 May 2023 | 11:57:37 16 May 2023 | 19 seconds |
| Total | 55 minutes 55 seconds | ||
