BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,227	0	T	G	30.1%	186.6 / 49.9	143	intergenic (+28/‑179)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base T (35/65); new base G (43/0); total (78/65)
Fisher's exact test for biased strand distribution p-value = 2.83e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 151 total aligned reads displayed.

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363

ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACTCCTGGGAAACCGGGCGtgttt                                                                                                                                         >  2:1617427/1‑140 (MQ=255)

ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCgttgtt                                                                                                                                         >  1:2267817/1‑140 (MQ=255)

aaccGACCAACTGCAAAGCTCTAAAGCTTACTATGTTGGGCTCAAACGGGTTGCTGAATCGGGGCAGAGCGGGTAAATTTTTAAATTTATCCCCTTTCAAAAAAAGAAAAACAAACACGCCTGGGAAACCGGGCGttttt                                                                                                                                         <  1:1749469/137‑1 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTTGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtttttttt                                                                                                                                      >  2:693427/1‑139 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACTGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGTGAAACCGGGCGTTTTtgtg                                                                                                                                      >  2:539551/1‑140 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtttttttt                                                                                                                                      >  2:1741021/1‑139 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtgttgttt                                                                                                                                      >  2:1749469/1‑139 (MQ=255)

    gAGCAACTGGAACGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTTTACCTATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGt                                                                                                                                     <  2:1757494/140‑1 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                    >  1:1841572/1‑140 (MQ=255)

      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACTAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTggtg                                                                                                                                   >  1:2105582/1‑137 (MQ=255)

        aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTtttttttttt                                                                                                                                 >  2:951994/1‑140 (MQ=255)

        aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTGTtttt                                                                                                                                 >  1:1965451/1‑140 (MQ=255)

        aaCTGGAAAGCTCGAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTGATACCGAGTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTAtt                                                                                                                                 >  2:871291/1‑140 (MQ=255)

          cTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGACTGGGAAACCTGGCGTGTTTTTGttagttt                                                                                                                               >  2:2629391/1‑135 (MQ=255)

           tGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTATATTTATACCGATTGAAACAACGTAATACAAAAACGCCTGGGAACCCGGGCGTGTTTTGGTTGttttt                                                                                                                              >  2:2104682/1‑140 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATGTaa                                                                                                                       >  2:452106/1‑140 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATt‑a                                                                                                                   >  2:30671/1‑140 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTGTTTGTTGTTTTTTATGTAATt‑a                                                                                                                   >  2:190053/1‑140 (MQ=255)

                       aaGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAACCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTGTTGTTTATGTTATt‑tct                                                                                                                 >  1:2097923/1‑140 (MQ=255)

                       aaGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTttatt‑aat                                                                                                                 >  1:2341167/1‑138 (MQ=255)

                       aaGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTTTGTAAAt‑aat                                                                                                                 >  1:1281460/1‑138 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATGTAAAT‑AAT‑Aaaaa                                                                                                           >  2:377279/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGttatt‑agagaaaa                                                                                                            >  1:315672/1‑133 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAAt                                                                                                          >  2:602276/1‑140 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAAt                                                                                                          >  2:1991332/1‑140 (MQ=255)

                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTATTTTATT‑AAT‑ACAAAATTTaaa                                                                                                    >  1:1577034/1‑140 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGTGCGTTGTTTTTTTTGTGTTTGTTTATTTAACT‑AAAAATTTGaaa                                                                                                    >  1:1643682/1‑140 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATGTAAAT‑AAG‑AAAAAATTTaaaa                                                                                                   >  2:1256254/1‑140 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTAATT‑TAT‑AAAAAATTTaaaa                                                                                                   >  1:2332287/1‑140 (MQ=255)

                                      ggCTGAAACGGCTTGCTTAATCGGTGAAGAGCGGGTAAATTTTTAATTTTATAACTATTGAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAAt                                                                                                 <  2:1643682/140‑1 (MQ=255)

                                      ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTATTTAAAT‑AAT‑AGAAAATTGaaaaaa                                                                                                 >  1:961912/1‑139 (MQ=255)

                                      ggCTGAAACGGCTTGCTGAATCGGTGAAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAAt                                                                                                 <  2:892623/140‑1 (MQ=255)

                                      ggCTCAAAGGGCTTGCTTAATGGGTGCATAGCGGGTAAATTTTTAAATTTATACCGATTTAAAAAAAGAAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAAt                                                                                                 <  1:1741021/140‑1 (MQ=255)

                                       gCTGAAACGGCTTGCTGACTGGGTGCAGCGCGGGGACATGATTAAAGTGATACCGATTGAAACAAGGGAAAACAAGAACGCCTCGGAACCCGGGGGGGGTGGGGTTCTGTTGTCGGTAATA‑ACT‑GCAAATTTGgaaaaga                                                                                                >  2:783576/1‑138 (MQ=255)

                                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATT‑TCT‑AAAAAATTGAAAAATa                                                                                                >  2:428675/1‑140 (MQ=255)

                                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTAAAT‑AAT‑ACAAATTTTaaaaaaa                                                                                                >  2:428479/1‑138 (MQ=255)

                                         tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATT‑AAA‑AAAAAATTTaaaaaaaag                                                                                              >  2:72709/1‑136 (MQ=255)

                                         tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATTTAATT‑AAT‑AAAAAATTTAAAAATACg                                                                                              >  2:1179043/1‑140 (MQ=255)

                                          gAAACGGCTTGCTGAATCGGGGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACgg                                                                                             <  2:1264666/140‑1 (MQ=255)

                                           aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATTTAATT‑ACT‑ACAAAATTTAAAAAAAAggg                                                                                            >  2:779134/1‑140 (MQ=255)

                                               ggCTTGCTGAATCGGTGCAGCGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGaaaa                                                                                        <  1:2695971/140‑1 (MQ=255)

                                                 cTTGCTGAATCGTTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTATTGTTCTTTTTTATGTAATT‑AAT‑AAAAAATTGAAAAATACGGGaaaaaa                                                                                      >  2:1431307/1‑139 (MQ=255)

                                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTGTTGTTATTTTTTATGTAATT‑AAT‑ACAAAATTTAAAAATAAGGGaaaaaa                                                                                      >  2:1200583/1‑139 (MQ=255)

                                                     gtGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCTATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATcc                                                                                  <  1:1746566/139‑1 (MQ=255)

                                                              tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATT‑ACT‑ACAAATTTTAAAAATAAGGGAAAAACATACCCTTTAAtt                                                                         >  2:2270437/1‑140 (MQ=255)

                                                                cAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATTTTAAT‑AAT‑AAAAAATTTAAAAAAAACGGAAAAAAAAACCAATTACTTca                                                                       >  1:2700028/1‑139 (MQ=255)

                                                                 agagCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                      <  1:1159934/140‑1 (MQ=255)

                                                                  gagCGGGGAAATTTTTAAATTTCTACCGATTGAAACACAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTCTTTTTTTTGTAATT‑AAT‑AAAAATATTAAAAAAACGGGTAAAAAAAACCATTTTCTTACaa                                                                     >  2:1186399/1‑140 (MQ=255)

                                                                   cgCGGGTAAATTTTTAAATTTATACCTTTTGAAAAAAATTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCaaa                                                                    <  1:73560/139‑1 (MQ=255)

                                                                       gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTGTTGTTGTTTTTTATTTTATT‑AAT‑ACAAAATTTAAAAAAAAGGGAAAAAAAACCCAAATAATTACAAAaaat                                                                >  1:2782411/1‑140 (MQ=255)

                                                                        gtaaaTTTTTAAATTTATACAGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAAAATTTAGTTTTTTTTTTATTTTTTATTTAATT‑AAT‑AAAAAATTTAAAAATAAAGGAAAAAAAAACAATTTAATTAAAAAATAtt                                                               >  2:1201254/3‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCTTTTTGTTGTTGTTTTTTATTTTAGT‑AAT‑ACAAATTTGAAAAATACTGGAAAAACATAGCATTTAATTCCAAAATAtt                                                               >  2:1355443/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTTTTTTTTTTTTTATT‑AATAAAAAATTTTAAAAAAACGGTAAAAAAAACCCGTTAATTTCAAAAaaat                                                                >  1:1192356/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATT‑ACT‑AACAATTTTAAAAATACGGGGAAAAAATACCATTTAATTACAAAAtttt                                                               >  2:1674412/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTTATT‑TAT‑AAAAATTTTTAAAATAATGGAAAAAAATACCATTTTATTAAAAAATAtt                                                               >  2:453989/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTAATT‑ACT‑AAAAAATTGAAAAATAAGGGAAAAACATACCAGTTTCTTACAAAataat                                                               >  2:312370/1‑138 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATT‑ACT‑AAAAATTTGTAAAATAAGGGTAAAACAAACCATTTAAGTACAAAATAtt                                                               >  2:144207/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTTTTGTTTTTTATGTAATT‑ACT‑AAAAAATTTAAAAAAAAGGGAAAAAAATACCAATTACTTACAAAataat                                                               >  2:1569841/1‑138 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTGTTTTTTGTTATT‑AAT‑TCAAATTTTTAAAATAAGGGAAAAACATCACATTTAATTCAAAAAGAtt                                                               >  1:2166274/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATTTAATT‑ACT‑CCAAATTTGAAAAATACGGGAAAAAACTCCCAATTACATCCAAAAAAtt                                                               >  2:2634787/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTGTTTATGTAAGT‑ACT‑ACAAAATTGAAAAATACGGGAAAAACATCCCATGTACTTCCCAAATAtt                                                               >  2:1664041/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATACCATTTAATTACAAAATAtt                                                               >  2:2015242/1‑140 (MQ=255)

                                                                                        ataCCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                               <  1:805419/140‑1 (MQ=255)

                                                                                            ctaTTCAAAAAACGAAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCC‑AAATATTTGATGGTAATTTTATGTAGGt                                          <  1:2394203/138‑1 (MQ=255)

                                                                                              aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTAATT‑AATAAAAAATTTAAAAAAAAAGGGAAATA‑ATACCAATTTTTTTGAAAAAATTTTATGGTGTTTTTATTTTGGtt                                         >  1:2502621/1‑139 (MQ=255)

                                                                                                tGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTTATT‑TAT‑TCAAATTTGAAAAATAAGGGAAAAAAATACCATTTAATTTGAAAATATTTGTTTGTAATTTTATGTTGGTtaa                                       >  2:1707330/1‑140 (MQ=255)

                                                                                                  acaCAAAGTAAAACAAAAACCCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:2105582/138‑1 (MQ=255)

                                                                                                  aaaaaaAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:2782411/140‑1 (MQ=255)

                                                                                                  aaaCAACCTAAACCAAAAACTACTGCGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATT‑AAT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  1:2190049/140‑1 (MQ=255)

                                                                                                  aaaCAAATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  1:1192666/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:382807/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:1030873/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:2601887/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:2428789/140‑1 (MQ=255)

                                                                                                       aaGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTa                                <  2:1577034/140‑1 (MQ=255)

                                                                                                            acaCAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1465984/138‑1 (MQ=255)

                                                                                                            aaccAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGACACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:2128992/137‑1 (MQ=255)

                                                                                                            aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:82407/140‑1 (MQ=255)

                                                                                                            aaaaaaaaACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:462399/140‑1 (MQ=255)

                                                                                                            aaaCAAAACCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:2457407/140‑1 (MQ=255)

                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1947284/140‑1 (MQ=255)

                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:648622/140‑1 (MQ=255)

                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1441376/140‑1 (MQ=255)

                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:30671/140‑1 (MQ=255)

                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1407872/140‑1 (MQ=255)

                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1319128/140‑1 (MQ=255)

                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:2284884/140‑1 (MQ=255)

                                                                                                             aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATT‑TCT‑ACAAATTTTAAAAATACGGGAAAAACATCCCATTTACTTCCAAAAAATTTTATTGTAATTTTATGTAGGTAAGAGTTTTATTTggg                          >  2:2500855/1‑139 (MQ=255)

                                                                                                             aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAAAT‑TAT‑ACAAAGTTTAAAAATTAGGGAAAAACCTAGCATTTACTTAAACAAAATTTTATGGTAATTTTATTTAGGTTAAATTTGTATTTggg                          >  2:848121/1‑139 (MQ=255)

                                                                                                             aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTTATT‑AAT‑ACAAATTTTTAAAATAAGGGAAAAAAAACCCAATTAATTCCAAAATATTTTATGGTAAATTTTTGTAGGTAAAATTTGTttttttg                          >  2:1319596/1‑137 (MQ=255)

                                                                                                             aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTAAATTTTATTTAATT‑ACA‑AAAAATATGAAAAATAAAGGAAAAACAAACCATTATCTTCAAAAATATATGATGGTAATTTTATGTAGGTAAAATTTGTaaatagc                          >  2:2854314/1‑134 (MQ=255)

                                                                                                                  aaaCGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTTATT‑TCT‑ACAAATTTGAAAAATACGGGAAAAACATACCATTTACGTCCCACATATTTGATGGTAATTTTTTGTTTGTAAAATTTTTTTTTGGGCGttg                     >  2:2124551/1‑139 (MQ=255)

                                                                                                                  aaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTTAAT‑ACT‑ACAAAATTGTAAAATAAGGGAAAAAAAAACCCTTTTATTCCAAAAAAAATTATGGTAAATTGTTGTAGGTAAAATTTGTTCTTggggcgta                     >  2:1939454/1‑134 (MQ=255)

                                                                                                                    aCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                   <  1:693427/140‑1 (MQ=255)

                                                                                                                    aCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                   <  1:1299537/140‑1 (MQ=255)

                                                                                                                      cccTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:453989/139‑1 (MQ=255)

                                                                                                                                     cgcttttttGTTCTCTTTTAGCTAATT‑ACT‑ACAAAGGTGCAACATACGCGACAAACATCCCATTTACGACCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  2:2480448/137‑1 (MQ=255)

                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:1861253/140‑1 (MQ=255)

                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:377279/140‑1 (MQ=255)

                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:1059548/140‑1 (MQ=255)

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`