BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP034943	132,099	T→C	V531A (GTA→GCA)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,099	0	T	C	99.5%	788.7 / NA	211	V531A (GTA→GCA)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (85/125); minor base G (1/0); total (86/125)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

CGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACT  >  NZ_CP034943/131963‑132230

cGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGt                                                                                                                                  <  1:2489255/140‑1 (MQ=255)

cGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGt                                                                                                                                  >  2:2838185/1‑140 (MQ=255)

 gTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGtt                                                                                                                                 >  1:1450512/1‑140 (MQ=255)

 gTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTGCGGACACGTTTGGAGCGAATTCGAACCAAACGAAGACGGCGCTTGCTGTGAATTTGAAAATGCGATTGTCGGTGGGGCAGtg                                                                                                                                 >  1:276354/1‑139 (MQ=255)

  tacggttGCAAAAGTTGAAGGTAATTTGGTACGTCATTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCTAATTCGAACCAAACGAAGATGGCTCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTc                                                                                                                                <  1:914071/134‑1 (MQ=255)

  tACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAACGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTc                                                                                                                                >  2:699196/1‑140 (MQ=255)

  tACCGGTGCAAAAGATGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTTAAAATGCGATTGTCGGTGGGGCAGTTc                                                                                                                                <  1:2321627/140‑1 (MQ=255)

     cGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCAc                                                                                                                             <  2:1249893/140‑1 (MQ=255)

     cGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCAc                                                                                                                             <  1:663423/140‑1 (MQ=255)

     cGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCAc                                                                                                                             <  1:780435/140‑1 (MQ=255)

     cGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCAc                                                                                                                             <  2:1422500/140‑1 (MQ=255)

     cGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCAc                                                                                                                             <  1:1251588/140‑1 (MQ=255)

       gTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCCGTTCCACGt                                                                                                                           >  2:2685485/1‑140 (MQ=255)

       gTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGt                                                                                                                           >  1:865329/1‑140 (MQ=255)

       gTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGt                                                                                                                           >  1:1977903/1‑140 (MQ=255)

       gTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGAGTGTCGGTGGGGGCGTTcgccgg                                                                                                                           >  1:2191010/1‑135 (MQ=255)

        tGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTg                                                                                                                          >  1:1100923/1‑140 (MQ=255)

        tGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTg                                                                                                                          >  2:2779584/1‑140 (MQ=255)

        tGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTg                                                                                                                          >  1:2032488/1‑140 (MQ=255)

        tGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTg                                                                                                                          >  2:131513/1‑140 (MQ=255)

        tGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTg                                                                                                                          >  1:85309/1‑140 (MQ=255)

        tGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTg                                                                                                                          >  2:2825055/1‑140 (MQ=255)

          cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                        <  2:1068059/140‑1 (MQ=255)

          cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                        <  2:2329290/140‑1 (MQ=255)

          cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                        <  1:1107937/140‑1 (MQ=255)

          cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAAGTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                        <  1:963285/140‑1 (MQ=255)

            aaaGTTGAATGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATa                                                                                                                      <  2:2548624/140‑1 (MQ=255)

            aaaGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATa                                                                                                                      >  2:2494519/1‑140 (MQ=255)

                    aGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAg                                                                                                              <  2:2044267/140‑1 (MQ=255)

                    aGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAg                                                                                                              <  1:1589776/140‑1 (MQ=255)

                         aaTTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGtt                                                                                                         >  1:183130/1‑140 (MQ=255)

                             cGTACGTCAGTGTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCGTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  1:2547123/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:889200/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:183130/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:1190237/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  1:2813895/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:2650250/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:2595560/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  1:114447/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:83229/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  1:2182207/140‑1 (MQ=255)

                             cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:916402/140‑1 (MQ=255)

                              gTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGc                                                                                                    <  2:197000/140‑1 (MQ=255)

                              gTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGACCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGc                                                                                                    <  2:1112059/140‑1 (MQ=255)

                                aCGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAg                                                                                                  <  2:487932/140‑1 (MQ=255)

                                     aGTCTGGTGGACGCGGTCAGTTCGGTCACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTg                                                                                             >  1:306673/1‑140 (MQ=255)

                                      gTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTgg                                                                                            >  1:1223508/1‑140 (MQ=255)

                                       tCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:2309528/130‑1 (MQ=255)

                                       tCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGa                                                                                           <  2:2408888/140‑1 (MQ=255)

                                       tCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGa                                                                                           <  2:905562/140‑1 (MQ=255)

                                                cgcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     <  2:1440394/121‑1 (MQ=255)

                                                cgcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                     >  1:1439580/1‑121 (MQ=255)

                                                cgcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACt                                                                                  >  2:1534469/1‑140 (MQ=255)

                                                cgcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACt                                                                                  >  1:2776171/1‑140 (MQ=255)

                                                 gcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACTTCACAGCTCTTCAAGCAGGTCTGGAAGATGCACtt                                                                                 >  2:2659239/1‑140 (MQ=255)

                                                 gcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACtt                                                                                 >  1:692545/1‑140 (MQ=255)

                                                 gcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACtt                                                                                 >  1:1157816/1‑140 (MQ=255)

                                                 gcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACtt                                                                                 >  2:2740639/1‑140 (MQ=255)

                                                   ggTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGa                                                                               <  1:484673/140‑1 (MQ=255)

                                                   ggTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGa                                                                               >  1:2040956/1‑140 (MQ=255)

                                                   ggTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGa                                                                               <  2:1611863/140‑1 (MQ=255)

                                                   ggTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAACAAGGCGCTAGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGa                                                                               >  2:2686091/1‑140 (MQ=255)

                                                     tCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGaaa                                                                             >  1:2299819/1‑140 (MQ=255)

                                                        gTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATg                                                                          <  1:192205/140‑1 (MQ=255)

                                                        gTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATg                                                                          <  1:2685620/140‑1 (MQ=255)

                                                          tCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGgt                                                                        <  2:403417/140‑1 (MQ=255)

                                                             gACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTa                                                                     >  2:290359/1‑140 (MQ=255)

                                                                 cGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTgg                                                                 >  2:1044503/1‑140 (MQ=255)

                                                                  gTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGc                                                                <  2:711830/140‑1 (MQ=255)

                                                                  gTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGc                                                                <  2:2264626/140‑1 (MQ=255)

                                                                   tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGACTTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCa                                                               <  1:2387117/140‑1 (MQ=255)

                                                                   tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                        >  2:2459759/1‑83 (MQ=255)

                                                                   tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCa                                                               >  2:1772685/1‑140 (MQ=255)

                                                                   tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCa                                                               >  1:812703/1‑140 (MQ=255)

                                                                   tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCa                                                               >  1:982973/1‑140 (MQ=255)

                                                                          cGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTcc                                                        <  1:2779584/140‑1 (MQ=255)

                                                                            aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCg                                                      >  2:549881/1‑140 (MQ=255)

                                                                            aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCg                                                      >  2:937074/1‑140 (MQ=255)

                                                                            aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATGTGAAAATGCGATTGTCGGTGTGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGTTGCACTTGAAAATGGTGTACTGGCAGGCTTCCCg                                                      >  1:1710188/1‑140 (MQ=255)

                                                                                       aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                           >  2:766670/1‑140 (MQ=255)

                                                                                       aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCGGTTCAAGCCGGTCTGGAAGATGCACTGGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                           >  1:1742833/1‑140 (MQ=255)

                                                                                                ggCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCaaa                                  >  2:605268/1‑140 (MQ=255)

                                                                                                    cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACtt                              >  1:1027880/1‑140 (MQ=255)

                                                                                                    cttgctgTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTCTCGACATTAAAGCAAAACtt                              >  2:1679248/1‑140 (MQ=255)

                                                                                                         tttgaatttgaaCATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                         <  2:2753798/140‑1 (MQ=255)

                                                                                                         tttgaatttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                         <  1:2495312/140‑1 (MQ=255)

                                                                                                         tttgaatttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                         <  1:2740639/140‑1 (MQ=255)

                                                                                                         tttgaatttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                         <  2:1139749/140‑1 (MQ=255)

                                                                                                         tttgaatttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                         <  2:859474/140‑1 (MQ=255)

                                                                                                         cgcgaatgtgaaACTGCGCTTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGGTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                         <  1:2161792/137‑1 (MQ=255)

                                                                                                          ttgaatttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGAt                        <  1:1086574/140‑1 (MQ=255)

                                                                                                             aatttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGt                     >  2:979956/1‑140 (MQ=255)

                                                                                                                ttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCt                  <  1:2659449/140‑1 (MQ=255)

                                                                                                                  gaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTa                >  1:2840119/1‑140 (MQ=255)

                                                                                                                   aaaaTGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                         >  2:196071/1‑130 (MQ=255)

                                                                                                                   aaaaTGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                         <  1:196071/130‑1 (MQ=255)

                                                                                                                     aaTGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCa             <  2:628107/140‑1 (MQ=255)

                                                                                                                     aaTGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCa             <  1:549881/140‑1 (MQ=255)

                                                                                                                           aTTGTCGGTGGGGCAGGTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGt       <  2:472264/140‑1 (MQ=255)

                                                                                                                                cGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACt  <  2:812638/140‑1 (MQ=255)

CGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACT  >  NZ_CP034943/131963‑132230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`