BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	196,535	C→G	7.1%	S99* (TCA→TGA)	cdaA →	diadenylate cyclase CdaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	196,535	0	C	G	7.1%	1025.9 / 12.4	437	S99* (TCA→TGA)	cdaA	diadenylate cyclase CdaA
Reads supporting (aligned to +/- strand): ref base C (144/229); new base G (33/3); total (204/233)
Fisher's exact test for biased strand distribution p-value = 2.73e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 444 total aligned reads displayed.

CAGCCAATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATGGGAGATTATATCGAG  >  NZ_CP034943/196398‑196674

cagccaATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCaa                                                                                                                                           >  2:677208/1‑140 (MQ=255)

cagccaATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCaa                                                                                                                                           >  2:1429390/1‑140 (MQ=255)

  gccaATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAgg                                                                                                                                         >  2:492037/1‑140 (MQ=255)

  gccaATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAgg                                                                                                                                         >  2:167811/1‑140 (MQ=255)

  gccaAGATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAgg                                                                                                                                         <  1:1441188/140‑1 (MQ=255)

         tttGGGGCTCAGCCCGGTTCAATGGCTGCTGGACCAAGCGTTACCCTGGGGCTTTTTAGCAATTTTTAGTATTTTTCAGCCTGAGGTTAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  2:86246/140‑1 (MQ=255)

         tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:3294349/140‑1 (MQ=255)

         tttGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  2:2346263/140‑1 (MQ=255)

         tttGGGCCTAAGCACGCTTCAATGGATGATGGACCAAGCGATAACATGGGGATTTTTAGGAATTTTTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  2:1493227/140‑1 (MQ=255)

         ttggggCCTCAGCACGCGTCAAGGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTGGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCa                                                                                                                                  <  1:2523193/137‑1 (MQ=255)

           tGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACg                                                                                                                                <  2:532489/140‑1 (MQ=255)

           tGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACg                                                                                                                                <  1:1913077/140‑1 (MQ=255)

             ggCCTCAGCACGCTTCTCTGGCTGATGGACCAAGCTATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTGGAACAGCTTGGCGGCGGCCGCTTTTTTTCAAGGAGCGGCACGcc                                                                                                                              <  2:2963299/140‑1 (MQ=255)

             ggCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGcc                                                                                                                              >  2:1464852/1‑140 (MQ=255)

             ggCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGcc                                                                                                                              >  2:1366300/1‑140 (MQ=255)

             ggCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGcc                                                                                                                              >  1:2314045/1‑140 (MQ=255)

             ggCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGcc                                                                                                                              >  2:2832003/1‑140 (MQ=255)

             ggCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAAAATGGGGATTTTTAGCAATTATTCTTATTTTTCAGCCTGAGCTGAGAAGAGCGCTGGTACAGCTTGGCCGCGGCCGCTTTTTTTAAAGGAGCGGCACGcg                                                                                                                              >  2:1593532/1‑139 (MQ=255)

                cTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCtgt                                                                                                                           <  1:388546/140‑1 (MQ=255)

                   aGCACGCTTCAATGGCTGATGGACCAAGCGATAAGATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGTggg                                                                                                                        >  2:804744/1‑139 (MQ=255)

                             aaTGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGGGGAGGACgcgccg                                                                                                              >  2:2298453/1‑138 (MQ=255)

                             aaTGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGTGGAGGAAGCgcag                                                                                                              >  2:3578668/1‑140 (MQ=255)

                               tGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCgcagca                                                                                                            >  1:2857859/1‑140 (MQ=255)

                                  gtGATGGACCATGCGATAACATGGTGATTTTTATCAATTATTATTATTTTTCAGCATGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCTGCCGCTATTTTTAAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaa                                                                                                         <  2:550228/139‑1 (MQ=255)

                                  cTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCCCGCCTGTGGCGGCAGCGCAGCAGaa                                                                                                         >  1:1025490/1‑140 (MQ=255)

                                  cTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaa                                                                                                         >  2:3730998/1‑140 (MQ=255)

                                  cTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCCGCACCCCTGTGGAGGGAGCGCAACAGaa                                                                                                         >  1:1124847/1‑140 (MQ=255)

                                  cTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTAAAGTAGCGGCACGCCTGGGGAGGAAGCGCACCACaa                                                                                                         >  1:164994/1‑140 (MQ=255)

                                    gATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTGAAGGAGCGGCACGCCGGTGGCGGAAGCGCAGCACaaaa                                                                                                       >  1:2082430/1‑140 (MQ=255)

                                    gATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaaaa                                                                                                       >  2:2060765/1‑140 (MQ=255)

                                    gATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGaaaa                                                                                                       >  1:2009990/1‑140 (MQ=255)

                                    gATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGGGGAGGAAGCGCAGCAGaaaa                                                                                                       >  2:2730541/1‑140 (MQ=255)

                                      tGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTGAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACa                                                                                                     >  2:1252350/1‑140 (MQ=255)

                                       ggACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAaaaaaa                                                                                                    >  2:2919988/1‑140 (MQ=255)

                                       ggACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGTGGAGGAAGCGCAGCAGAAAACaa                                                                                                    >  2:1966355/1‑140 (MQ=255)

                                       ggACCAAGCGATAACATGGGGATTTTTAGCAATTATTAGTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGATTTTTTTCAAGGAGCGGCACGCCGGGGGAGGAAGCGCAGCAGAAAACaa                                                                                                    >  2:3681915/1‑140 (MQ=255)

                                            aaGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCgg                                                                                                                                    <  2:2870456/103‑1 (MQ=255)

                                              gCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGc                                                                                             <  1:3701061/140‑1 (MQ=255)

                                              gCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGc                                                                                             <  1:533294/140‑1 (MQ=255)

                                                   aaCATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGGAGCGCAGCAGAAAAAAAATGAGGcccaca                                                                                        >  1:765949/1‑136 (MQ=255)

                                                         gggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAAATGAGGCCATCACAAAAg                                                                                  >  2:1756084/1‑140 (MQ=255)

                                                          ggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGACGCGCAGCAGAAAAAAATTGAGGCCATCACAAAAGc                                                                                 >  2:1203595/1‑140 (MQ=255)

                                                          ggATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGATGCCATCACAAAAGc                                                                                 >  2:4004357/1‑140 (MQ=255)

                                                           gATTTTTTTCAATTTTTATTATTTTTCAGCCTGTGCTGATTAGTGCTCTTGAACAGCTTGGGCGCGGCCTCTTTTTTTCAATGAGCGGCACGCCTGTGGAGGAAGAGCAGCAGAAAACAATTGAGGCCATCACAATAGca                                                                                <  2:3466734/140‑1 (MQ=255)

                                                           gATTTTTAGCTATTTTTTTTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACTCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGca                                                                                <  2:2506506/140‑1 (MQ=255)

                                                           gATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGca                                                                                <  1:2946130/140‑1 (MQ=255)

                                                             tttttAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGcaat                                                                              <  1:1511288/140‑1 (MQ=255)

                                                             tttttAGCAATTATTATTATTTTTCAGCCGGAGCTGAGAAGAGCGCGTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGcaat                                                                              <  1:137087/140‑1 (MQ=255)

                                                                     aaTTATTATTATTTTTCAGCGTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATtata                                                                      <  2:608906/140‑1 (MQ=255)

                                                                     aaTTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATtata                                                                      <  1:1157239/140‑1 (MQ=255)

                                                                     aaTTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTCAACAGCTTGGCCGCGGCCGCTTTTTTACAAGTAGCGGCACGCCTGTGGAGTAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATtata                                                                      <  1:1297457/140‑1 (MQ=255)

                                                                         attattattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGTGGAGGAAGCGCAGCAGAAAACAATTTAGTCCATCACAAAAGCAATCAATTATATTGc                                                                  >  2:2978602/1‑140 (MQ=255)

                                                                             ttattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCACGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  1:3534641/140‑1 (MQ=255)

                                                                             ttattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  2:3193245/140‑1 (MQ=255)

                                                                             ttattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  1:3379625/140‑1 (MQ=255)

                                                                             ttattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  1:3155994/140‑1 (MQ=255)

                                                                             ttattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  1:2594597/140‑1 (MQ=255)

                                                                             ttattTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  1:1393775/140‑1 (MQ=255)

                                                                               attTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACg                                                            <  1:1847670/140‑1 (MQ=255)

                                                                                     cAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGtat                                                      <  1:2142661/140‑1 (MQ=255)

                                                                                     cAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGtat                                                      <  1:3616294/140‑1 (MQ=255)

                                                                                       gCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCGGGGGAGGAAGCGCAGCAGAAAAAAATTGAGGCCATCACAAACGCAATAAATTATTTGGCGAAACGCCGTagag                                                    >  2:111039/1‑140 (MQ=255)

                                                                                         ctgagctgagAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGgc                                                  <  2:73525/140‑1 (MQ=255)

                                                                                          agagctgagAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCATTTTTTCAACGAGCGGCACGCCTGTGGAGCAAGCGCAGCAGAAAACAATTGAGGCCATCACAAACGCAATCAATTATATGGCGAAACGCCGTATAGgcg                                                 <  1:3042996/139‑1 (MQ=255)

                                                                                                 ataagaGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCctgct                                          <  2:3782777/138‑1 (MQ=255)

                                                                                                  gaagaGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCctgctg                                         <  1:89542/140‑1 (MQ=255)

                                                                                                  gaagaGCGCTTGAACAGCTTGGCCGCGGCCGCTTTATTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGCAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCctgctg                                         <  1:2790309/140‑1 (MQ=255)

                                                                                                      agCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTGAAGGAGCGGCACGCCGGGGGAGGCAGCGCAGCAGAAAACAATTGAGGCCCGCACAAAAGCAAACAAATATATTGCGAAACGCCCTAGAGGCGCCCTGCTGACCa                                     >  1:3184684/1‑140 (MQ=255)

                                                                                                      agCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              <  1:2669074/115‑1 (MQ=255)

                                                                                                      agCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGaaa                                                              >  2:2669074/1‑115 (MQ=255)

                                                                                                        cgcTTGAACAGCTTGGCCGCGGCCGCTTTTTTTGAAGGAGCGGCACGCCTGTGGGGGAAGCGCAGCAGAAAAAAAATTATGCCATCACAAAAGCAATAAATTATATGGCGAAACCCCGGATATGCGCCCAGCTGACCaat                                   >  1:3958372/1‑138 (MQ=255)

                                                                                                           tttaaCAGCTTGGCCGCGGCCTCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  2:3038514/137‑1 (MQ=255)

                                                                                                           tttaaCAGATTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  2:2423724/137‑1 (MQ=255)

                                                                                                           ttGATCAGCTTGGCCGGGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  2:907703/140‑1 (MQ=255)

                                                                                                           ttGACCAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAAGGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:934359/140‑1 (MQ=255)

                                                                                                           ttGAACAGCTTGGCCGCGGCCGCTTTTTTTGAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:2993621/140‑1 (MQ=255)

                                                                                                           ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:581390/140‑1 (MQ=255)

                                                                                                           ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:2488364/140‑1 (MQ=255)

                                                                                                           ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:3441155/140‑1 (MQ=255)

                                                                                                           ttGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:3239006/140‑1 (MQ=255)

                                                                                                           gtGAACCGCTTGGCCGCGGCCGCTTTTTTTCCAGGAGCGGCACGCCTGTGGACGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:1736793/139‑1 (MQ=255)

                                                                                                           gtGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAg                                <  1:218495/139‑1 (MQ=255)

                                                                                                             gAACAGCTTGGCCGCGGCCGCTTTTTTTGGAGGAGCGGCACCCCGGGGGCGGCAGCGCCGCAGAAAACAATTGATGCCATCACAAAAGCAAACAATTATATTGCGAAACGCCCTGTAGGCGCCCTGGTGAACCATGAGCg                              >  1:108968/1‑140 (MQ=255)

                                                                                                             gAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGATCGGCACGGCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCCACACAAAAGCAATCAAATATATTGCGAAACGCCGTATATGAGACCTGCTGACCATTGAGCg                              >  2:185662/1‑140 (MQ=255)

                                                                                                              aaCAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCgg                             <  2:1883173/140‑1 (MQ=255)

                                                                                                                   tttGGGCGGGGCAGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATAc                        <  2:3971439/139‑1 (MQ=255)

                                                                                                                   cTTGGCCGCGGCCGCTTTTTTTCAAGGCGCGGCACGCCTGTGGAGGGAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATAc                        >  2:3130628/1‑140 (MQ=255)

                                                                                                                   cTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCCTCACAAAAGCAATCAATTATATTGCGAAACGCCGTATAGGCGCCCGGCTGACCATTGAGCGGGATAc                        >  2:1521723/1‑140 (MQ=255)

                                                                                                                   cTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATTTGCGCCCTGCTGACCAGTGAGCGGGATAc                        >  2:1823758/1‑140 (MQ=255)

                                                                                                                   cTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATAc                        >  2:3881836/1‑140 (MQ=255)

                                                                                                                   cTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATAc                        <  2:2566179/140‑1 (MQ=255)

                                                                                                                   cTTGGCCGCGGCCGCTTTTTTTAAAGGAGCGGCACGCCGGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATAc                        >  2:2814067/1‑140 (MQ=255)

                                                                                                                          gcggccgcTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATg                 <  1:825598/140‑1 (MQ=255)

                                                                                                                            ggccgcTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAACGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATggg               <  1:1939695/140‑1 (MQ=255)

                                                                                                                            ggccgcTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATggg               <  2:3946495/140‑1 (MQ=255)

                                                                                                                             gccgcTTTTTTTCGCGGGGCGGCACGCCTGGTGAAGAAGCGCAGCAGAAAACGATTGAGGGCCTTACAAAAGCAAACACTTATATTGCGAAACGCCGTATAGGCGCCCTGCTGACCCTTGAGCGGGGTACTGGAATgggg              >  2:3387394/1‑139 (MQ=255)

                                                                                                                             gccgcTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATGGga              >  2:1282140/1‑140 (MQ=255)

                                                                                                                             gccgcTTTTTTTCAAGGAGCGGCACGCCGGGGGCGGAAGCGCAGCAGAAAACAATTGATGCCCTCACCAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCGGCTGACCATTGAGCGGGATACCGGACTGGga              >  2:105836/1‑140 (MQ=255)

                                                                                                                             gccgcTTTTTTTAAAGGAGCGGCACGCCGGGGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCCCAAAAGCAATCAATTATATGGCGAAACGCCGGATAGGCGCCCGGCTGACCAGTGAGCGGGATACCGGAATGGga              >  2:456356/1‑140 (MQ=255)

                                                                                                                                         cAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATGGGAGATTATATCgag  <  1:731007/140‑1 (MQ=255)

CAGCCAATATTTGGGCCTCAGCACGCTTCAATGGCTGATGGACCAAGCGATAACATGGGGATTTTTAGCAATTATTATTATTTTTCAGCCTGAGCTGAGAAGAGCGCTTGAACAGCTTGGCCGCGGCCGCTTTTTTTCAAGGAGCGGCACGCCTGTGGAGGAAGCGCAGCAGAAAACAATTGAGGCCATCACAAAAGCAATCAATTATATGGCGAAACGCCGTATAGGCGCCCTGCTGACCATTGAGCGGGATACCGGAATGGGAGATTATATCGAG  >  NZ_CP034943/196398‑196674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`