BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,702	C→T	19.6%	A182V (GCC→GTC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,702	0	C	T	19.6%	293.9 / 73.6	173	A182V (GCC→GTC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (72/63); new base T (3/28); total (75/98)
Fisher's exact test for biased strand distribution p-value = 5.35e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGG  >  NZ_CP034943/607567‑607840

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGa                                                                                                                                        >  1:924502/1‑140 (MQ=255)

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                        <  1:4004087/140‑1 (MQ=255)

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                        >  2:1273872/1‑140 (MQ=255)

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                        <  1:2578010/140‑1 (MQ=255)

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGATCGGGGACTCCGGACCAACCGGAGCTACAGGAGCAAACGGGGATGCGGGa                                                                                                                                        >  1:622025/1‑140 (MQ=14)

    ggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAg                                                                                                                                    <  1:2567661‑M1/16‑1 (MQ=255)

        gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGAAGGCGGAGCAGCCgg                                                                                                                                <  2:1587696‑M1/20‑1 (MQ=255)

        gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGCACGTCGGACCAGCCgg                                                                                                                                <  2:2471568‑M1/20‑1 (MQ=255)

          cggaccaaccggagcgacgggagccacgggggctactggggacgtcggaccagccggagcgacaggagctcccggtgaagccggaccaaccggcgcgacgggcgccacaggggctactGGGTACGTCGCACCAGCCGGAg                                                                                                                              <  2:2300631‑M1/22‑1 (MQ=255)

          aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                              <  2:2245395/140‑1 (MQ=255)

             accaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctacgGGGGACGTCGGACCAGCCGGAGCGa                                                                                                                           <  2:314803‑M1/25‑1 (MQ=255)

                cccTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACGGGCGAAGCCGGAGCAGCCGGAGCCCCAGGAGCTCCGGGCGACGCAGGAGCTACCGGAGCGACAGGAGCTACCGGCGAACCCGGACCAGCCGGAGCGACAg                                                                                                                        <  1:3634389/138‑1 (MQ=14)

                 ccgggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  1:3939354/137‑1 (MQ=255)

                 aCTGGGGAAGCCGGACCAGCCGGGGCGAAAGGACATAGCTGGGAAGCCGGACCACCCGGCGCGACTAGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCAGCCGGcgcgtgggg                                                                                                                       >  2:3949107/1‑135 (MQ=11)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCGACC‑GGGCCGCCGGCGCAGCCGGAGCGACAGGa                                                                                                                      >  2:1643770/1‑140 (MQ=14)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGAGCGACCGGAGCGCCCGGGGCAGCCGGACAAACCGGAGCgcccgg                                                                                                                       >  2:609040/1‑135 (MQ=11)

                 aCTGGCGAAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  1:936746/140‑1 (MQ=14)

                   cggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                     <  2:3008840/139‑1 (MQ=39)

                      agcgacgggagccacaggggcaactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctccgGGCGACGGCGGACCAGCCGGAGCGTCAGGAGCTa                                                                                                                  <  2:2899062‑M1/34‑1 (MQ=255)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCGAcc                                                                                                                >  1:803072/1‑140 (MQ=255)

                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                                >  1:1596108/1‑140 (MQ=255)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                              >  2:2185551/1‑140 (MQ=255)

                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGagcgaccggagcgaccgg                                                                                                              >  2:2407432/1‑140 (MQ=255)

                          gCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGAGCAGCCTGAGCGACATCAGCTACCGGGCAAGCCGGACCAACCGGTGCGAGAGGAGCTACCGCGGAAGCAGGACCAGCCGGGGCGACAGGAGCTACCgg                                                                                                              >  1:3485701/1‑140 (MQ=14)

                           ccGGACCCGCAGGAGCGAGTTGAGCTACCGGGGTTGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             <  1:3340074/140‑1 (MQ=14)

                               agccacaggggctactgcccacgtcggcccagccgcagcgacaggagctaccggggaagccggaccaaacggagcgtggggagccacaggggctactGGGGAAGTCGTACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         >  1:3949107‑M1/98‑140 (MQ=255)

                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGacg                                                                                                         >  1:1868117/1‑138 (MQ=14)

                                    ccGGAGCGCCTGGAGCCTCGGGGGACCCCCGACCAGACGGAGCCACAGGAGCTCACGGTGAACCCGGACCAACCGGAGCCACAGGAGCTACAGGCCAAGCGGGACCAGCGGGAGCCACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  1:1799264/140‑1 (MQ=9)

                                    ccGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  1:2795030/140‑1 (MQ=255)

                                        aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAGGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                                                <  1:1235956/140‑1 (MQ=255)

                                                   cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGTAGCGACAGGGGACGACGGACCAACCGGAGCCACg                                                                                     >  1:113854/1‑140 (MQ=14)

                                                     aCCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                   <  2:768044/140‑1 (MQ=255)

                                                     aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                   <  2:349043/140‑1 (MQ=255)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAACAGGAACAGCCGGAGCGACAGTAGCAACCGACGAATCCAGACCACACGCAGAGACAGGac                                                                                 >  1:1026467/1‑139 (MQ=11)

                                                         ggcaaGCTGGACCACCGGGAGCCCCAGGAGATACGGGCGACCCCGGACCTACCGGCGACCCAGGACCTCCCGGGGATTCCGGACCCCCCGGAGCTACAGGTGCTCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                                               <  2:966804/137‑1 (MQ=2)

                                                             aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCCGCCGGACCAACAGGAGCGACAGGAGCGACCGGGGACGCCGTACCAGACTGAGCGTCAGGAGCTCGCGCGCAAGCCGGTCCACCCGGAGCGGCGGGAGCCCCAg                                                                           >  1:1960971/1‑140 (MQ=14)

                                                             aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACTCCGGACCAGCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                                           >  1:2962203/1‑140 (MQ=255)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCCGGCCCAGCCGCAGCGACAGGAGCGCCCGGCGCCGCCGGACAAGCGGGTGCGCCGGGCGCGACAgg                                                                          >  2:170225/1‑140 (MQ=11)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                          <  1:1041743/140‑1 (MQ=255)

                                                              cccGGACCCCCCGGAGCGCCAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCCCCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAgg                                                                          <  1:1431403/139‑1 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACTCCGGACCAGCCGGAGCGACTGGAGCAACCGGGGCAGCCGGACCAACCGGAGCGACGGGATCCACAggag                                                                        >  2:1998197/1‑138 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGCCGGGAGCCACAggcg                                                                        >  2:3943661/1‑138 (MQ=255)

                                                                            agccccAGGAGCTCCCGGTGAACCCGGAGCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            <  1:2751041/135‑1 (MQ=17)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGTGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            >  2:2892494/1‑140 (MQ=255)

                                                                            aGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            <  1:3684362/140‑1 (MQ=255)

                                                                            aGCCACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                            <  1:3707886/140‑1 (MQ=255)

                                                                              ctaccggggaagctggaccagctggagcgacgggagcgACAGGAGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGGGCTACTGGGGACGTc                                                          >  1:72225‑M2/39‑140 (MQ=255)

                                                                                 ccggggacgccggacccaccggagccacgggagccaccggcgcttctGGCGACGCCGGAGCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                       >  1:1738283‑M1/48‑140 (MQ=255)

                                                                                  cggggacgccgcaccacccggagcgacgggagccTCAGGGGCTCCCGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                      <  2:666187‑M2/106‑1 (MQ=255)

                                                                                  aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                                     <  2:1741196/93‑1 (MQ=37)

                                                                                      gacgccggcccagccggagccaccgccgccACAGCCGCTACCGGGGACGGCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                                  <  2:1868117‑M2/110‑1 (MQ=255)

                                                                                      gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                                  <  2:3663399/140‑1 (MQ=255)

                                                                                       cgtccGGGGCCGCCGGTCCAACCGGTGCTACAGGTGCTTCCGGGGAAGCGGGACCCCCGGGAGCGACAGGAGCTAGCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                                                 <  2:737555/137‑1 (MQ=18)

                                                                                       cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACACCGGACCACCCGGAGCGACGGGAGCCCCAGGGGCTCCTGGGGACGGCGGCCCAGcc                                                 >  2:1125589/1‑140 (MQ=17)

                                                                                         gccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACcagcggg                                               <  1:518771‑M1/101‑4 (MQ=255)

                                                                                         gccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                               <  2:2633917‑M1/101‑1 (MQ=255)

                                                                                         gccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                               <  1:518341‑M1/101‑1 (MQ=255)

                                                                                         actggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGCCCAGCCGGAGCGACGGGAGCCACCGGGGCGACGGGGGACGCCGGAGCCGCCgg                                               >  2:656548/4‑140 (MQ=11)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             >  2:2675495/1‑110 (MQ=255)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  1:2675495/110‑1 (MQ=255)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                               >  2:2250113/1‑140 (MQ=255)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                               <  1:2227877/140‑1 (MQ=255)

                                                                                          ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCTGCAGGAGCGACAGGGGAAGCCGGACCAACCGGAGCCTCGGGAGCCACAGGGGCTACTGGGGAGCTCGGACCAGCCGGa                                              >  2:1424556/1‑140 (MQ=18)

                                                                                           cgcaccagcgggagcgacgggagcgACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                             <  2:72225‑M2/115‑1 (MQ=255)

                                                                                            ggggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                   >  2:1926187/1‑101 (MQ=35)

                                                                                            ggggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                   <  1:3924948/101‑1 (MQ=35)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCAACCGGCGACGCCGGCCCACCCGGAGCGCCGGGAGCCACAGCCGCGACGGGGGACGTCGGACCAGCCGGAGCgcc                                         >  2:3132731/1‑138 (MQ=17)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                  >  2:1677780/1‑51 (MQ=35)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                  <  1:1677780/51‑1 (MQ=35)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGAACAACCGGAGCTAGGGTAGCCACAGGGGGTACGGGGGACCTCGGACCGGCCTGAGCGACAgg                                      >  2:803670/1‑140 (MQ=17)

                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                   <  2:3842468/38‑1 (MQ=33)

                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCCCAGGCGCGACGGGCGACGGCGGACCAGCCGCAGCGCCAGCAGCTAGCgg                             >  2:1475506/1‑140 (MQ=17)

                                                                                                            cgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                            <  1:3105407‑M1/120‑1 (MQ=255)

                                                                                                            ccGGAGCGACAGGCGCTACCGGCGAAGCCGGAGCTGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                            <  2:459913/140‑1 (MQ=18)

                                                                                                                cgCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGCCGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                        <  1:1932502/139‑1 (MQ=255)

                                                                                                                aGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAACCAGGACCAACCGCAGCGACGGGAGCCACAGGGGCTACTCGGGAAGTCGGAGCAGCCGGAGCGCCAGGAGGTCCCggggccg                        >  1:97608/1‑137 (MQ=18)

                                                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGCAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                        >  1:1176545/1‑140 (MQ=255)

                                                                                                                  ccacaggggctactGGGGACGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  1:2176340‑M1/29‑1 (MQ=255)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGCAGCGCCGGGAGCCCCAGGGGCGACTGGGGCCGTCGGACCTGCCGGAGGGACCCGAGCGACCGGGGACGCggg                    >  2:1244850/1‑140 (MQ=18)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGCGCAACAGGCGCCGGCGGAGCTGCCTGAGCGGCATGAGCTCCGGGGGAAGcagc                    >  2:1343848/1‑137 (MQ=14)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                    >  2:1341913/1‑140 (MQ=255)

                                                                                                                       ggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                 <  1:3828165‑M1/131‑1 (MQ=255)

                                                                                                                         ggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa               <  1:1218284‑M1/133‑1 (MQ=255)

                                                                                                                          gctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc              <  2:3425615‑M1/134‑1 (MQ=255)

                                                                                                                             actGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg           <  2:1928080‑M1/137‑1 (MQ=255)

                                                                                                                             actGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         >  1:3906379‑M1/4‑46 (MQ=255)

                                                                                                                             actGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  2:3906379‑M1/43‑1 (MQ=255)

                                                                                                                             actGGCGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg           >  2:3687773‑M1/4‑140 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGCGACGTCGGACCAGCGGGAGCGACAGCGGCTACCGTGGAATCCTTACCAACggg           >  2:928253/1‑140 (MQ=18)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCAACAGGGGAAGTCGGACCAGCCGTAGCGCCAGGAGCTACCGGGGAATCCTGACCaccacg           >  2:1418899/1‑135 (MQ=17)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg           >  2:3346498/1‑140 (MQ=255)

                                                                                                                                ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGc        >  1:240021/1‑140 (MQ=255)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             >  1:339819/1‑68 (MQ=40)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                             <  2:339819/68‑1 (MQ=40)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa          <  2:3924499/135‑1 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcgg   >  2:2427533/1‑138 (MQ=255)

                                                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcggt  >  1:1359389/1‑137 (MQ=255)

                                                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg  <  1:2185551/140‑1 (MQ=255)

                                                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg  >  2:1045246/1‑140 (MQ=255)

                                                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg  >  2:3197999/1‑140 (MQ=255)

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGG  >  NZ_CP034943/607567‑607840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`