Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,838 A→G 14.1% T227T (ACA→ACG EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,8380AG14.1% 1007.9 / 113.3 382T227T (ACA→ACGEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (142/178);  new base G (20/33);  total (166/216)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 403 total aligned reads displayed.

AAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACAC  >  NZ_CP034943/607699‑607977
                                                                                                                                           |                                                                                                                                           
acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                                                                             >  1:917215/5‑140 (MQ=255)
acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                                                                             >  1:2650888/5‑140 (MQ=255)
acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                                                                             >  1:2620952/5‑140 (MQ=255)
acgtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACAGGGCACGTCGGACCTGCCGTAGCGAAAGCAGCTACCGGGGAAGCCGTACCAACCGGAGCGTCa                                                                                                                                             >  1:93663/5‑140 (MQ=17)
  gtcGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                                           >  2:2770261/3‑140 (MQ=255)
    cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                                                                                                         >  2:1456662/1‑140 (MQ=255)
    cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAg                                                                                                                                         >  2:40926/1‑140 (MQ=255)
    cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                         >  2:150545/1‑140 (MQ=255)
    cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                         >  1:791102/1‑140 (MQ=255)
    cGGACCAGCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGATCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCCACAGGAGCTACCGGCGAAGCCGGACCAACCGCAGCGACAGGAg                                                                                                                                         >  2:2685677/1‑140 (MQ=17)
       agcgacgggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCa                                                                                                                                      >  1:3617551/8‑140 (MQ=255)
           gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACagg                                                                                                                                  <  2:2180969‑M2/140‑4 (MQ=255)
            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACTGCAGCCACgggg                                                                                                                                 >  2:1747139/1‑139 (MQ=255)
            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggg                                                                                                                                 <  1:1054343‑M2/140‑5 (MQ=255)
            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggg                                                                                                                                 <  2:3113501‑M2/140‑5 (MQ=255)
            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                                                                                 >  2:1320339/1‑140 (MQ=255)
            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                                                                                 >  1:2368222/1‑140 (MQ=255)
            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                                                                                 >  2:3432617/1‑140 (MQ=255)
             aggaGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACagggg                                                                                                                                >  1:3338501‑M2/2‑135 (MQ=255)
                aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCca                                                                                                                             >  1:3854307/1‑140 (MQ=255)
                     cAGGAGCTACCGGGGAAGCCGGACCAACCGGATCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAGCCGGACCAACCGGCGCGACGGGAGCCACgggggctccgggg                                                                                                                        <  2:3179356‑M2/140‑14 (MQ=255)
                     cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCCCGGGAGCcacaggc                                                                                                                        >  2:1269054/1‑140 (MQ=255)
                     cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                        >  2:2575521/1‑140 (MQ=255)
                      aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggca                                                                                                                       >  2:75576/1‑140 (MQ=255)
                      aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGCACCAGCCGGAGCGTCAGGAGCTACCGGGGAAGCCGGACCAACGCGAGCGACGGGAGCCACaggggctaggggtg                                                                                                                       <  1:3004499‑M2/140‑15 (MQ=255)
                          gcgaCCCGGGAAGCCCGACCACCCGGACCGACGGCAGCCACAGGGGCTAATGGGGACGTCGGTCCAGCGGGAGCGTCAGGAGCTACGTGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                   <  2:1176545/137‑1 (MQ=21)
                             aCCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctcctggggacgtcgc                                                                                                                <  1:743515‑M2/140‑22 (MQ=255)
                               cGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                              >  2:397647/1‑140 (MQ=255)
                                  ggAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGCGACGTCGGACCAGCGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctaggggggaggtcgcaccag                                                                                                           <  1:557946‑M2/140‑27 (MQ=255)
                                   gAAGCCGGACCAGCCGGCGCGACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCACAGGCCCAGGcgcagcggc                                                                                                          >  2:3218344/1‑137 (MQ=14)
                                    aggCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAACAGCTggg                                                                                                         >  2:1095806/3‑140 (MQ=255)
                                      gCCGGACCAACCGGAGCGACGGGAGCCACATGGGCTACTGGGGACGTCGGACCAGCCGGAGGGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctaatggggacggaggaccagccgg                                                                                                       <  2:3288449‑M2/140‑31 (MQ=255)
                                      gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACGGGAGCCACaggggctcctggggacgtcggaccagccgg                                                                                                       <  2:3662474‑M2/140‑31 (MQ=255)
                                      gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACaggggctactggggaaggcggagcagccgg                                                                                                       <  2:1587696‑M2/140‑31 (MQ=255)
                                      gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGtt                                                                                                       >  1:3799294/1‑140 (MQ=255)
                                        cGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                     <  1:3218344/140‑1 (MQ=255)
                                        cGGACCAACCGGAGCGACGGGAGCCACGGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCAACCGGCGCGACGGGCGCCACaggggctactgggtacgtcgcaccagccggag                                                                                                     <  2:2300631‑M2/140‑33 (MQ=255)
                                        cGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                     >  2:2117991/1‑140 (MQ=255)
                                        cGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                     >  2:868234/1‑140 (MQ=255)
                                           aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTCTCCg                                                                                                  >  1:634504/1‑140 (MQ=255)
                                           aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCg                                                                                                  >  2:2896335/1‑140 (MQ=255)
                                           aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCg                                                                                                  >  1:2747382/1‑140 (MQ=255)
                                             cAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGaa                                                                                                <  1:2899062/140‑1 (MQ=255)
                                               cccGGAGCGCCGGGAGACACCGGGGCTACTGGGGACGTGGTACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAAtc                                                                                              <  1:1902338/139‑2 (MQ=25)
                                               cccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  1:1343848/139‑1 (MQ=255)
                                               aCCGGAGCGCCGGGAGCCCCAGGGGCTCCCGCGGACGACGGACCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  1:3993277/140‑1 (MQ=25)
                                               aCCGGAGCGACGGGAGCCACAGGGTCTACTGGGGACGTCAGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  1:1244850/140‑1 (MQ=255)
                                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              >  1:1307552/1‑140 (MQ=255)
                                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                              <  1:2575521/140‑1 (MQ=255)
                                                    aGCGACGGGAGCCACAGGGGCTAGTGGTGAAGTCGGTCGTGCCGGAGCTTCAGTAGCTCCCGGCGATGCCGGAGAACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                         <  1:2300276/140‑1 (MQ=21)
                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                         <  1:1643770/140‑1 (MQ=255)
                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                         <  1:2117991/140‑1 (MQ=255)
                                                     gCGACCGGAGCCACCGGGGCAGCCGGTCCAGCCGGCCCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGta                                                                                        <  1:1507624/140‑1 (MQ=21)
                                                       gACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGGCGGACCAACGGGAGCGCCGGGAGCCCCGGGCGCCACAGGCACAGGCGCAGCTGGGTTCTCGGAATACGGGtaga                                                                                      >  2:3044854/1‑138 (MQ=21)
                                                         cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCAGACTACGGGtatatc                                                                                    >  2:2388531/1‑139 (MQ=255)
                                                         cGGGAGCCACAGGGGCGCCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAGGGGtatata                                                                                    >  2:53788/1‑140 (MQ=25)
                                                           ggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatata                                                                                  <  1:2153179/140‑1 (MQ=255)
                                                           ggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatata                                                                                  <  1:428590/140‑1 (MQ=255)
                                                                       gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                      >  2:2502956/1‑140 (MQ=255)
                                                                        cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCt                                                                     <  1:1475506/140‑1 (MQ=255)
                                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                                                                             <  2:2076001/66‑1 (MQ=255)
                                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGt                                                                                                        >  2:934363/1‑103 (MQ=255)
                                                                              gggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACg                                                               <  1:1587696/140‑1 (MQ=255)
                                                                               ggaagccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACgg                                                              <  2:789066/134‑1 (MQ=255)
                                                                               ggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACgg                                                              <  1:2052150/140‑1 (MQ=255)
                                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACTGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                             <  1:1420577/140‑1 (MQ=255)
                                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                             <  1:1747139/140‑1 (MQ=255)
                                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                             <  1:3432617/140‑1 (MQ=255)
                                                                                gACGTCGGACCAGCAGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                             <  1:1122107/140‑1 (MQ=255)
                                                                                 aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                            >  2:1031826/1‑140 (MQ=255)
                                                                                 aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                            <  1:2140452/140‑1 (MQ=255)
                                                                                  cGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAg                                                           <  2:3617551/140‑1 (MQ=255)
                                                                                   gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatata                                                                                  >  1:1031852/1‑116 (MQ=255)
                                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGTCGCAACAGGCGAAGGAGCACCTGCGTTATCCGAATAAGCGACTATATAAGACGTACGCGCACAAGCgaaggcta                                                        >  2:1635487/1‑133 (MQ=12)
                                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAg                                                                         <  2:1963243/123‑1 (MQ=255)
                                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCta                                                        >  1:1732176/1‑140 (MQ=255)
                                                                                      ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCtat                                                       <  1:2502956/140‑1 (MQ=255)
                                                                                       gcccAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCtata                                                      <  1:3680749/138‑1 (MQ=255)
                                                                                              gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTg                                               >  2:2955007/2‑140 (MQ=255)
                                                                                                        ggAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCAtttt                                     <  2:2049490/140‑1 (MQ=255)
                                                                                                            cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGAt                                 <  1:313225/140‑1 (MQ=255)
                                                                                                               ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGta                                                                                        >  1:1813982/1‑82 (MQ=255)
                                                                                                               ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCa                              <  1:701672/140‑1 (MQ=255)
                                                                                                                cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                             <  1:851120/140‑1 (MQ=255)
                                                                                                                cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                             <  1:2427533/140‑1 (MQ=255)
                                                                                                                cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                             <  1:2932203/140‑1 (MQ=255)
                                                                                                                 ggggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAAc                            <  2:1717089/140‑1 (MQ=255)
                                                                                                                   ggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGt                                                                                                        <  2:3532067/62‑1 (MQ=255)
                                                                                                                   ggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGt                                                                                                        >  1:3532067/1‑62 (MQ=255)
                                                                                                                   ggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAg                          >  2:188086/1‑140 (MQ=255)
                                                                                                                    gAAGCCGGACCAACCGGAGCGACTGGAGCCACAGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAgg                         <  1:1998197/140‑1 (MQ=255)
                                                                                                                    gAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAgg                         <  1:3943661/140‑1 (MQ=255)
                                                                                                                         cGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATaa                    >  2:3701508/1‑140 (MQ=255)
                                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                   <  1:222316/140‑1 (MQ=255)
                                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                   <  2:1786518/140‑1 (MQ=255)
                                                                                                                                aCCGGCGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc             <  2:1660660/140‑1 (MQ=255)
                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc             <  2:3869908/140‑1 (MQ=255)
                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc             <  2:654175/140‑1 (MQ=255)
                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc             <  1:1716792/140‑1 (MQ=255)
                                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa        <  1:515188/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACAC  >  NZ_CP034943/607699‑607977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: