BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	814,356	T→G	16.6%	H340Q (CAT→CAG)	nosA →	nitric oxide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	814,356	0	T	G	16.6%	482.5 / 52.4	227	H340Q (CAT→CAG)	nosA	nitric oxide synthase
Reads supporting (aligned to +/- strand): ref base T (72/112); new base G (41/2); total (113/114)
Fisher's exact test for biased strand distribution p-value = 1.58e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

AGCAAGGTGTCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCTTA  >  NZ_CP034943/814217‑814494

aGCAAGGTGTCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCat                                                                                                                                            <  1:1599299/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGCACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGCCTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  <  1:393356/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  <  2:619947/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  <  2:3177064/140‑1 (MQ=255)

          cAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCAcc                                                                                                                                  <  1:1516208/140‑1 (MQ=255)

           aGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCg                                                                                                                                 >  2:1755220/1‑140 (MQ=255)

           aGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCg                                                                                                                                 >  2:370381/1‑140 (MQ=255)

               tCGTTGACCATCATACAGCGGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCAATTTCACCCGCTGCCACTCATATCTTCCACCGCTcc                                                                                                                             <  1:3243505/140‑1 (MQ=255)

                cGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCt                                                                                                                            >  2:656425/1‑140 (MQ=255)

                cGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCt                                                                                                                            >  2:2330753/1‑140 (MQ=255)

                 gTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTa                                                                                                                           >  1:1000920/1‑140 (MQ=255)

                  tGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTcc                                                                                                                             >  2:1144719/1‑137 (MQ=255)

                  tGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATAGCTTCCACCGTTCCTAt                                                                                                                          >  2:4025631/1‑140 (MQ=255)

                       catcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGACGAAGCGGGCAGTAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACaa                                                                                                                     >  2:3968809/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACt                                                                                                                   >  2:1909710/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACt                                                                                                                   >  2:357613/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACt                                                                                                                   >  2:2296651/1‑140 (MQ=255)

                         tcatACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACt                                                                                                                   >  1:2796123/1‑140 (MQ=255)

                          catACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTc                                                                                                                  >  2:93235/1‑140 (MQ=255)

                            tACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCaa                                                                                                                >  2:1343811/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                           >  2:36201/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                           >  2:2894634/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                           >  1:1078336/1‑140 (MQ=255)

                                 ccGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGtt                                                                                                           >  1:3676068/1‑140 (MQ=255)

                                   gCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTaa                                                                                                         >  2:3112421/1‑140 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                        >  2:3565082/1‑140 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                        >  2:3876249/1‑140 (MQ=255)

                                    cAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAg                                                                                                        >  1:3807107/1‑140 (MQ=255)

                                     aaGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGc                                                                                                       >  2:3088208/1‑140 (MQ=255)

                                            tttAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTa                                                                                                <  2:3370871/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGCCGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCGACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  1:3921093/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCTCTGCCACTCATATCTTCCACCGTTCCTATTACAACTTAATATTTAAGCCGAATTACTTTTAt                                                                                         <  2:1026219/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  2:165934/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  1:3296407/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  1:2434765/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  2:1078336/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  1:913928/140‑1 (MQ=255)

                                                   ggTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  1:143083/140‑1 (MQ=255)

                                                     tttGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGACTTACTTTTATCa                                                                                       >  1:3624979/1‑140 (MQ=255)

                                                     tttGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCa                                                                                       >  1:784182/1‑140 (MQ=255)

                                                         aagaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGAt                                                                                   <  1:2844172/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  2:1599326/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  2:3295369/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  2:3771268/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:1020568/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:225294/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:3209134/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:3478025/140‑1 (MQ=255)

                                                          agaaCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATa                                                                                  <  1:357613/140‑1 (MQ=255)

                                                             aCAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGc                                                                               >  1:188641/1‑140 (MQ=255)

                                                              cAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGcc                                                                              >  1:2991761/1‑140 (MQ=255)

                                                              cAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGcc                                                                              >  1:3396457/1‑140 (MQ=255)

                                                              cAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGcc                                                                              >  1:1107772/1‑140 (MQ=255)

                                                               aggaggAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCt                                                                             <  2:3223787/140‑1 (MQ=255)

                                                                ggaggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCtt                                                                            >  2:786133/1‑140 (MQ=255)

                                                                 gaggaAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTa                                                                           >  1:3901090/1‑140 (MQ=255)

                                                                      agaagCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAGTCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGt                                                                      >  2:1234944/1‑140 (MQ=255)

                                                                           cGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTAt                                                                                         <  1:3780387/116‑1 (MQ=255)

                                                                            gggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAaacaa                                                                <  1:687425/140‑1 (MQ=255)

                                                                            gggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTAAATCGTTAAGCCGATTTACTTTTATCAGGATATGCCTTACGAGTAaacaa                                                                <  1:1689055/140‑1 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAaacaac                                                               >  1:942324/1‑140 (MQ=255)

                                                                             ggCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAaacaac                                                               >  1:2958242/1‑140 (MQ=255)

                                                                                         aCGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTg                                                   >  1:2281058/1‑140 (MQ=255)

                                                                                         aCGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACTACGGAAAATCGGTg                                                   >  1:2979026/1‑140 (MQ=255)

                                                                                         aCGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTAATTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTg                                                   >  1:1042154/1‑140 (MQ=255)

                                                                                          cGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTGTGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGa                                                  <  1:1399965/140‑1 (MQ=255)

                                                                                          cGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGa                                                  <  1:1343811/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  1:2226707/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                >  1:887518/1‑140 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  1:1571333/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  2:2703700/140‑1 (MQ=255)

                                                                                            ggggACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATg                                                <  1:257000/140‑1 (MQ=255)

                                                                                                aCTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCt                                            >  2:2912340/1‑140 (MQ=255)

                                                                                                aCTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCt                                            >  1:992572/1‑140 (MQ=255)

                                                                                                aCTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCt                                            >  1:51432/1‑140 (MQ=255)

                                                                                                   ggACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCa                                         <  1:2330753/140‑1 (MQ=255)

                                                                                                         ggCTGATTCCGCCGATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTg                                   >  1:1046426/1‑140 (MQ=255)

                                                                                                           cTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACACCTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCg                                 <  1:630206/140‑1 (MQ=255)

                                                                                                              aTTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGa                              <  1:1171605/140‑1 (MQ=255)

                                                                                                                tCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGAtt                            <  2:2841878/140‑1 (MQ=255)

                                                                                                                tCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGAtt                            <  2:3311737/140‑1 (MQ=255)

                                                                                                                tCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGAtt                            <  2:151661/140‑1 (MQ=255)

                                                                                                                   gccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGaa                                                                                                   >  2:2426437/1‑66 (MQ=255)

                                                                                                                   gccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtg                         <  2:605870/140‑1 (MQ=255)

                                                                                                                   gccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtg                         <  2:942324/140‑1 (MQ=255)

                                                                                                                   gccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtg                         <  2:2958242/140‑1 (MQ=255)

                                                                                                                   gccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtg                         <  2:218816/140‑1 (MQ=255)

                                                                                                                    ccgATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtgt                        >  1:2122240/1‑140 (MQ=255)

                                                                                                                    ccgATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtgt                        >  1:1374248/1‑140 (MQ=255)

                                                                                                                    ccgATTTCACCCGCTGCCACTCAGATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCtgt                        >  1:2731829/1‑140 (MQ=255)

                                                                                                                       aTTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGAc                     <  1:3884388/140‑1 (MQ=255)

                                                                                                                                  tGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAGTCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCgg          <  1:669572/140‑1 (MQ=255)

                                                                                                                                  tGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCgg          <  2:2784019/140‑1 (MQ=255)

                                                                                                                                  tGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCgg          <  1:3655942/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  1:183064/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  2:3673350/140‑1 (MQ=255)

                                                                                                                                   gCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTCTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGt         <  2:2137346/140‑1 (MQ=255)

                                                                                                                                   acagcTCATATCTTCCACCGTTCCTATGACAACTCAATAGTTAAGCCTAATTACTTTTATCAGTATAAGACTTACGAGTAAACAACGGAAATTCTTTTATGGACTTCAATTATTATTTATTATGTGACAGAGACGTCgtt         <  2:2639711/136‑3 (MQ=255)

                                                                                                                                        tCAGATCTTCCAACGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCt    <  2:3854565/140‑1 (MQ=255)

                                                                                                                                         cATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCtt   <  1:3567650/140‑1 (MQ=255)

AGCAAGGTGTCAGCATCGTGGACCATCATACGGCCGCAAGCCAGTTTAAACGGTTTGAAGAACAGGAGGAAGAAGCGGGCAGAAAGCTGACGGGGGACTGGACGTGGCTGATTCCGCCGATTTCACCCGCTGCCACTCATATCTTCCACCGTTCCTATGACAACTCAATCGTTAAGCCGAATTACTTTTATCAGGATAAGCCTTACGAGTAAACAACGGAAAATCGGTGATGGCCTTCAAGGCTGCGGGATTCTGTGACAGAGACGTCGGTAACCTTA  >  NZ_CP034943/814217‑814494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`