Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,097,399 C→A 5.4% Q48K (CAA→AAA)  EO946_RS05675 → MarR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,097,3990CA 5.4% 619.9 / 8.7 206Q48K (CAA→AAA) EO946_RS05675MarR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (102/87);  new base A (2/14);  total (105/101)
Fisher's exact test for biased strand distribution p-value = 1.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

ATACAGATCATACGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTT  >  NZ_CP034943/1097262‑1097538
                                                                                                                                         |                                                                                                                                           
aTACAGATCATACGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCaa                                                                                                                                           <  1:3629658/140‑1 (MQ=255)
aTACAGATCATACGAAACGCAACATTTTTGAAGTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGTGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCaa                                                                                                                                           <  1:2183174/140‑1 (MQ=255)
      aTCATACGAAACGCAACATTTTTTAATTAAATCCTTAGATAATTAATCAGCACGAGAAATGGGAGGTGATCATTAAATCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCg                                                                                                                                     >  2:1117356/1‑140 (MQ=255)
      aTCATACGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCg                                                                                                                                     >  1:978493/1‑140 (MQ=255)
      aTCATACGAAACGCAAAATTTTTTAATTATATGCGGAGCTAATTAATCAGCAGGAGAAATGGGAGTGGCTCATTAAAGACTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCg                                                                                                                                     >  2:572416/1‑140 (MQ=255)
         aTACGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATg                                                                                                                                  >  1:2100277/1‑140 (MQ=255)
           aCGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGTCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATGATCGATGAc                                                                                                                                >  1:3399584/1‑140 (MQ=255)
           aCGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGAc                                                                                                                                >  2:1737115/1‑140 (MQ=255)
           aCGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGAc                                                                                                                                >  1:2193515/1‑140 (MQ=255)
            cGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACa                                                                                                                               <  1:3462031/140‑1 (MQ=255)
            cGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACa                                                                                                                               <  1:3864454/140‑1 (MQ=255)
            cGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACa                                                                                                                               <  1:1879467/140‑1 (MQ=255)
            cGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACa                                                                                                                               <  2:1424709/140‑1 (MQ=255)
              aaaCGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTTAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGa                                                                                                                             <  2:2663243/140‑1 (MQ=255)
              aaaCGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGa                                                                                                                             <  2:2694129/140‑1 (MQ=255)
              aaaCGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGa                                                                                                                             <  2:665791/140‑1 (MQ=255)
                aCGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGaaa                                                                                                                           >  1:3481252/1‑140 (MQ=255)
                aCGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGaaa                                                                                                                           >  1:2716353/1‑140 (MQ=255)
                    aaCATTTTTGAAGTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCa                                                                                                                       >  2:1956316/1‑140 (MQ=255)
                         ttttGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGttc                                                                                                                  >  2:2763027/1‑140 (MQ=255)
                             gAATTATATGCGGAGCTCATTCATCAGCCGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGttctttc                                                                                                              <  2:2317959/140‑1 (MQ=255)
                               aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                            <  2:2730370/140‑1 (MQ=255)
                               aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                            <  2:1152157/140‑1 (MQ=255)
                               aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                            <  2:924834/140‑1 (MQ=255)
                               aTTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTtctttctt                                                                                                            <  2:425912/140‑1 (MQ=255)
                                  atatGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTtgtg                                                                                                         <  2:3224727/140‑1 (MQ=255)
                                  atatGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTtgtg                                                                                                         <  1:3164974/140‑1 (MQ=255)
                                  atatGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTtgtg                                                                                                         <  1:3700122/140‑1 (MQ=255)
                                   tatGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTgtgt                                                                                                        <  1:1121743/140‑1 (MQ=255)
                                       cGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTggg                                                                                                    <  2:2104595/140‑1 (MQ=255)
                                       cGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTggg                                                                                                    <  1:157986/140‑1 (MQ=255)
                                       cGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTggg                                                                                                    <  2:3753663/140‑1 (MQ=255)
                                          aGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTGAATTATCGATGACAGAAATTAATGTTCTTTCTTGTGTTGGGTAc                                                                                                 >  2:717510/1‑140 (MQ=255)
                                          aGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGGGTTGGGGAc                                                                                                 >  2:3474981/1‑140 (MQ=255)
                                           gCTCATTCATCAGCAGGAGACATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCACTTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACa                                                                                                <  1:2954762/140‑1 (MQ=255)
                                           gCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACa                                                                                                <  1:31090/140‑1 (MQ=255)
                                           gCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACa                                                                                                <  1:3367482/140‑1 (MQ=255)
                                           gCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACa                                                                                                <  1:1821034/140‑1 (MQ=255)
                                            cTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACaa                                                                                               >  1:1499374/1‑140 (MQ=255)
                                            cTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACaa                                                                                               >  1:1345199/1‑140 (MQ=255)
                                               attcatCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACTGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGa                                                                                            >  2:2768797/1‑140 (MQ=255)
                                               attcatCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTgg                                                                                                     <  1:1400735/131‑1 (MQ=255)
                                                ttcatCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATAGATTACAGAAATTGATGTTATTTCTTGTGTTGGGGACAATGat                                                                                           <  2:3273735/140‑2 (MQ=255)
                                                     cagcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaat                                                                                      <  2:3724997/140‑1 (MQ=255)
                                                     cagcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaat                                                                                      <  2:3840823/140‑1 (MQ=255)
                                                     cagcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaat                                                                                      <  1:3080352/140‑1 (MQ=255)
                                                      agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatc                                                                                     >  2:3493598/1‑140 (MQ=255)
                                                      agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatc                                                                                     >  2:82496/1‑140 (MQ=255)
                                                      agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTAAATTATCGATGACAGAAATTCATGTTCTTTCTTTTGTTGGGGACAATGAACaaatc                                                                                     >  2:2891859/1‑140 (MQ=255)
                                                         agGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaat                                                                                  >  2:2801327/1‑140 (MQ=255)
                                                         agGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaat                                                                                  >  1:3314989/1‑140 (MQ=255)
                                                           gagaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                >  1:168044/1‑140 (MQ=255)
                                                           gagaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                >  2:1324102/1‑140 (MQ=255)
                                                            agaAATGGGAGGGGCTCATTAAAGCCTTGTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTc                                                                               <  2:2808229/140‑1 (MQ=255)
                                                             gaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACa                                                                                                <  2:1260316/122‑1 (MQ=255)
                                                             gaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACa                                                                                                >  1:1260316/1‑122 (MQ=255)
                                                             gaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGAAATAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATTTCa                                                                              >  1:867483/1‑140 (MQ=255)
                                                              aaaTGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCAc                                                                             <  2:3235215/140‑1 (MQ=255)
                                                                aggggAGGGGCTCATTAAACCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGTAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGt                                                                           <  2:2368634/138‑1 (MQ=255)
                                                                aTGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGTGGACAATGAACCAATCAATGTCACGt                                                                           >  2:3423758/1‑140 (MQ=255)
                                                                aTGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGt                                                                           >  2:2570545/1‑140 (MQ=255)
                                                                   ggAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGTGGAAAATGAACCAATCAATGTCACGTCTa                                                                        >  1:82527/1‑140 (MQ=255)
                                                                     aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                      >  2:3620390/1‑140 (MQ=255)
                                                                     aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                      >  1:2508482/1‑140 (MQ=255)
                                                                        ggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCa                                                                   >  1:1700937/1‑140 (MQ=255)
                                                                        ggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCa                                                                   >  1:345458/1‑140 (MQ=255)
                                                                          cTCATTAAAGCCTTTTTGGCTGACGAATTGGAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGa                                                                 >  2:907731/1‑140 (MQ=255)
                                                                              ttAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaaaa                                                             <  2:1499374/140‑1 (MQ=255)
                                                                                   gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCATTCAATGTCACGTCTATTGCAGAAAAAATGaa                                                        <  1:2783238/140‑1 (MQ=255)
                                                                                   gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                        <  2:4904/140‑1 (MQ=255)
                                                                                       ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTc                                                                               >  1:3815901/1‑113 (MQ=255)
                                                                                       ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                    >  1:519491/1‑140 (MQ=255)
                                                                                       ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                    >  1:2173284/1‑140 (MQ=255)
                                                                                       ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                    >  1:2758258/1‑140 (MQ=255)
                                                                                          tGGCTGACGAATTGAATAATCTCGATGTCGAGAAGGGGTGTAAATGTCAATTATCGATGACAGAAATTCATGTTCTTTGTTGTGTTGGGGACATTGAACCAATCAATGTCACGTCTTTTGCAGAAAAAATGAATACGACa                                                 <  2:1800201/140‑1 (MQ=255)
                                                                                          gggCTGACGAAGTGACGCAGCTCCCTGGCGAGCACGGGAGTAAAAGTCACTTACCGAAGACCGAACTTCATGTTCGTTTTGGTGTGGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACa                                                 <  2:3186810/139‑1 (MQ=255)
                                                                                            gCTGACTATTTTAAGACGCTCGATGTCGAGCACGGTAGTAATTATAATTTATCTCTTACAGAATTTCATTTTTTTTGTTGTGTTGGGTAAAATGAACAAATAAATGTCACGTTTATTGCATAAAAAATTAATACGACaaa                                               <  2:786380/140‑1 (MQ=255)
                                                                                              tGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAg                                             >  2:3523715/1‑140 (MQ=255)
                                                                                               gACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGc                                            >  2:618348/1‑140 (MQ=255)
                                                                                               gACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGc                                            >  2:1051832/1‑140 (MQ=255)
                                                                                                aCGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCg                                           >  2:3867998/1‑140 (MQ=255)
                                                                                                 ctaaTTGAATACGCGCGATTTCGAGCAGGGTATTAAATCTAAATTATCGATGACATAAATTATTGTTGTTTGTTGTGTTGGGGAAAATCAACAAATCAATGTCACGTCTTTTCCATAAAAAATGAATACGACAAAAGCGa                                          <  2:3335106/138‑1 (MQ=255)
                                                                                                 cGAATTGAAGACGCTCTATGGCGAGCAGGGGAGTAAATATCAATTATCTATGACAGAAATTCTTTTTGTTTCTTGTGTTGGGGACAATTAACAAATCATTGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGa                                          <  2:3028084/140‑1 (MQ=255)
                                                                                                          gaagCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATcgc                                 >  1:167072/1‑140 (MQ=255)
                                                                                                             gCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTTCAGAAAAAATGAATACGAAAAAAGAGACTGTATcgcgaa                              >  2:2445970/1‑138 (MQ=255)
                                                                                                             gCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCa                              >  1:91720/1‑140 (MQ=255)
                                                                                                             gCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCa                              >  1:2852497/1‑140 (MQ=255)
                                                                                                              ctctttGTCGAGCACGGGTTTAAATATAATTTATCTATCACATATATTATTGTTTTTTGTTGTTTTGGGCAAATTGAACAAATAATTTTCACGTCTTTTGCATAAAAAATGAATACCACAAAAGCGACTGTATCGCGCAt                             <  1:3013458/135‑1 (MQ=255)
                                                                                                              cTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCAt                             >  1:2569721/1‑140 (MQ=255)
                                                                                                                   tGTCGAGCACGGTATTAAATCTAAATTATCGATGACACAAATTCTTGTTCTTTCTTGTGTTGGGCAAAATCAACCAAACAATGTCACGTCTTTGGCAAAAAAATTTAACCCCACAAAAGCGACTGTATCGCGCATCAGca                        <  1:1956316/140‑1 (MQ=255)
                                                                                                                             ggtattAAATATCAATTATCGATCACACAATTTCTTTTTGTTTTTTGTTTTGGGCAAAATCAAAAAATAAATGTCACGTATATTGCACAAAAAATTAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCt              <  1:837781/135‑1 (MQ=255)
                                                                                                                             gggAGTAACCCTCATTTATCGATCACAGAATTTCTTGTTGTTTCTTGTGTGGGGGACAATCAACCAATCAATGTCACGTCTATTGCAGAAAAATTGAATACGAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCt              <  2:2040791/140‑1 (MQ=255)
                                                                                                                             gggAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCt              >  1:120447/1‑140 (MQ=255)
                                                                                                                                    aaTCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGcgcg       >  1:2398667/1‑140 (MQ=255)
                                                                                                                                     aTCTCAATTATCGATGAAATAAATTCTTGTTCTTTCTTGTTTTGGGTACAATGAACCAATCAATGTCACTTATATTGCATAAAAAATTAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg      <  2:1394411/140‑1 (MQ=255)
                                                                                                                                     aTCTAATTTATCTATGACATAATTTATTTTTGTTTTTTGTGTTGGGCACAATCAACCAATCAATGTAACGTCTATTCAAAAAAAAATCAATCCCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg      <  1:2345770/140‑1 (MQ=255)
                                                                                                                                     aTCTAAATTATCTATGACAGAAAGTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATAAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg      <  2:2242522/140‑1 (MQ=255)
                                                                                                                                     aTCTAAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCAGGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg      <  2:2953810/140‑1 (MQ=255)
                                                                                                                                       ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt    >  2:646330/1‑140 (MQ=255)
                                                                                                                                       ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt    >  1:2293830/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ATACAGATCATACGAAACGCAACATTTTTGAATTATATGCGGAGCTCATTCATCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTT  >  NZ_CP034943/1097262‑1097538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: