Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,514,854 T→G 10.9% intergenic (+58/+1) EO946_RS07915 → / ← EO946_RS07920 YlaH‑like family protein/YlaI family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,514,8540TG10.9% 403.4 / 10.9 180intergenic (+58/+1)EO946_RS07915/EO946_RS07920YlaH‑like family protein/YlaI family protein
Reads supporting (aligned to +/- strand):  ref base T (61/93);  new base G (24/2);  total (85/95)
Fisher's exact test for biased strand distribution p-value = 2.70e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

TCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCG  >  NZ_CP034943/1514723‑1514989
                                                                                                                                   |                                                                                                                                       
tcgCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCGGCTGTtttttt                                                                                                                                 >  1:1584655/1‑140 (MQ=255)
tcgCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAttttt                                                                                                                                 >  1:214948/1‑140 (MQ=255)
tcgCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTAttttt                                                                                                                                 >  1:3127417/1‑140 (MQ=255)
 cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAGGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTGTTGTATGGTAAAGCGGCCGCTTTAtttttt                                                                                                                                >  2:128918/1‑140 (MQ=255)
 cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAtttttt                                                                                                                                >  1:3772874/1‑140 (MQ=255)
 cgctgcgctgATTTTAATCATTTACAAAATTCGGCGGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGACGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTAtttttt                                                                                                                                >  1:2348466/1‑140 (MQ=255)
 cgctgcgctgATTTGTATCATTTACAAAATTCGGGTGTATCAGTCGTAAATGGGACAGTCTGCAAAATCATAAACAGACTCAGCTGAGAAGCTGTGGGTTTTGTTGGGTTGGGTCTGGTACAGCGGCGGGTGTAtttgtg                                                                                                                                >  2:2940211/1‑137 (MQ=255)
  gctgcgctgATTTTAATCATTTACAAAATTCGGCTTTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTTTATGGTAAATCGGCCGCTTGATTTTTTg                                                                                                                               >  2:3140888/1‑140 (MQ=255)
  gctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTg                                                                                                                               >  2:3549296/1‑140 (MQ=255)
  gctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTAttttttt                                                                                                                               >  2:2418935/1‑139 (MQ=255)
      cgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGAcgt                                                                                                                           >  2:2735571/1‑138 (MQ=255)
        ctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGGatttt                                                                                                                         >  1:757246/1‑140 (MQ=255)
        ctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGAgtttt                                                                                                                         >  2:114061/1‑140 (MQ=255)
           aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGTctgtttgt                                                                                                                      >  2:3668611/1‑138 (MQ=255)
           aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGActttttct                                                                                                                      >  2:824504/1‑140 (MQ=255)
           aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGActttttct                                                                                                                      >  1:2967582/1‑140 (MQ=255)
           aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTGTTTAcgttttgt                                                                                                                      >  1:3148678/1‑138 (MQ=255)
           aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCGTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGActttttgt                                                                                                                      >  2:3673798/1‑138 (MQ=255)
                caTCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTGTGTGTGTTGTATGGTAAAGCGGCCGCTTTATTTTTTGTCTTTTTCtttttt                                                                                                                 >  2:1816163/2‑140 (MQ=255)
                aaTCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCATCGGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTTTTTTTTGTGtttttttttttt                                                                                                                 >  1:642612/1‑140 (MQ=255)
                aaTCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCtttttt                                                                                                                 >  2:444967/1‑140 (MQ=255)
                aaTCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCtttttt                                                                                                                 <  2:3478675/140‑1 (MQ=255)
                    aTTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGc                                                                                                             <  1:2444440/140‑1 (MQ=255)
                    aTTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGc                                                                                                             <  1:3743080/140‑1 (MQ=255)
                    aTTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGc                                                                                                             <  1:3742410/140‑1 (MQ=255)
                    aTTTACAAAATTCGGCTGTATCAGTAGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGc                                                                                                             <  1:2748660/140‑1 (MQ=255)
                     tttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACTTTTTGgttttttttgt                                                                                                            >  1:3004546/1‑137 (MQ=255)
                     tttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCt                                                                                                            >  1:949322/1‑140 (MQ=255)
                     tttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGAGTTTTTGTTTTTTTTGCt                                                                                                            >  1:1849051/1‑140 (MQ=255)
                         cAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTcc                                                                                                        <  1:707545/140‑1 (MQ=255)
                              ttCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTGTATTTTTTGTCTTTTTGTTTTTTTTGCTGTCCCGGGt                                                                                                   >  2:3788986/1‑140 (MQ=255)
                                             cGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTc                                                                                    <  2:2121030/140‑1 (MQ=255)
                                                aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGATGTCCCGGGTGAAAAGTGAATTTTCCCg                                                                                 >  2:2032635/1‑140 (MQ=255)
                                                aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAATTTTTTACTTTTTGTTTTTTTTGCGTTACCCGGTGAAAATTGAATTTTCCCg                                                                                 >  1:1896348/1‑140 (MQ=255)
                                                aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTGTTGTGTTGTGTATGGTAAAGCGGCCGCTGTATTTTTTGACGTTTTGTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTACCg                                                                                 >  2:136284/1‑140 (MQ=255)
                                                     ggACAGTCTGCAAAATCATAACAAGACTCAGCTGAGAAGCTGAGTCATTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTGTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGtttac                                                                            <  1:1918966/140‑3 (MQ=255)
                                                      gACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGTCTTTTTCTTTTTTTTGGTTTACCGGGTGAAAAGTTTATTTTACCGttttaa                                                                           >  1:2533294/1‑138 (MQ=255)
                                                      gACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAATTTTTGACTTTTTCATTTTTTTGCTGTCCAGGGTGAAAATTTAATATTCACGttttaa                                                                           >  2:727370/1‑138 (MQ=255)
                                                        cAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGc                                                                         <  2:3166214/140‑1 (MQ=255)
                                                          gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGGTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTACCGTTTTGAGcag                                                                       >  2:719877/1‑138 (MQ=255)
                                                          gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACGTTTTGTTTTTTTTTGTTTGCCGGGTGAAAAGTGAATTTTCCCGGTTTAAGGCg                                                                       >  1:833773/1‑140 (MQ=255)
                                                          gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCg                                                                       <  2:1191622/140‑1 (MQ=255)
                                                          gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACTTTTTCTTTTTTTTGCTGTACCGGGTGAAAAGTGAATTTTACCGTTTTGAGCCg                                                                       >  2:1299206/1‑140 (MQ=255)
                                                          gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTATTTTTGTTTTTTGTATGTTAAAGCGGCCGCTTTTTTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCg                                                                       <  2:918982/140‑1 (MQ=255)
                                                               cAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTTTTTTTTTTGTTGTTGTTTTTTTTGCTGTCCCGGGTGTAAATTGTATTTTACCGGTTTGAGCCGgtctt                                                                  >  1:1886790/1‑140 (MQ=255)
                                                                 aaaTCATAAATAGACTCAGCGGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTGGCTGGCCCGGGTTAAAAGTGAATTTTCCGGTTTTCAGCCGTtcttct                                                                <  2:1289995/140‑1 (MQ=255)
                                                                 aaaTCATAAAAAGACTCAGGTTCGAAGCTGAGTATTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTtcttct                                                                <  2:489513/140‑1 (MQ=255)
                                                                     cATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGGCGGTTTGTTTTTTTTGGTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGGTCTTCTGttt                                                            >  1:2815859/1‑140 (MQ=255)
                                                                     cATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGAGGTTTTGTTTTTTTTTGTGTCGCCGGTGAAAAGTGAATTTTACCGGTTTAAGCCGTTGGTCTGttt                                                            >  1:329693/1‑140 (MQ=255)
                                                                     cATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCGCGGTTTCAGCCGTTCTTCTGttt                                                            >  2:2580705/1‑140 (MQ=255)
                                                                       tAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATGTTTTGACGTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTACCGTTTTAAGCCGTTCGTGTGttttt                                                          >  2:3564882/1‑140 (MQ=255)
                                                                          aaaGACTCAGCTGAGCAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGGGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTgg                                                       <  2:1660360/140‑1 (MQ=255)
                                                                           aaGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTGTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                      <  2:175587/140‑1 (MQ=255)
                                                                           aaGACTCAGCTGAGAAGCTGAGTATTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                      <  2:1474621/140‑1 (MQ=255)
                                                                              aCTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGGTTTTTTTTGTCTTTTTGTTTTTTTTTGTGTCGCGGGGGGAAAGTGAATTTTGCCGGTTTGAGGCGGTGTTGTGTTTTTTggggac                                                   >  1:1250517/1‑137 (MQ=255)
                                                                              aCTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGGATGGTAAAGCGGCCGCTTTAGTTTTTGACTTTTTCTTTTTTTTTCTGTACCGGGTGAAAAGTTAATTTTCCCGGTTTCAGCCGTTCTTATGTTTTTTTGGCaa                                                   >  2:306282/1‑140 (MQ=255)
                                                                              aCTCAGCTGAGAAGCTGAGTCTTTGTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACTTTTTCTTTTTTTTGCTGTACCGGGTGAAAAGTTAATTTTCCCGTTTTCAGCCGTTCTTGTGTTTTTTTTGCaa                                                   >  2:604426/1‑140 (MQ=255)
                                                                                tCAGCTGAGAAGCTGAGTCTTTTTTTGTTTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGCCTTTTTCTTGTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATc                                                 <  2:3899283/140‑1 (MQ=255)
                                                                                tCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATc                                                 <  2:1960535/140‑1 (MQ=255)
                                                                                tCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATc                                                 <  2:257676/140‑1 (MQ=255)
                                                                                  aGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGGATGGTAAAGCGGCCGCGTTATTTTTTTACGTTTTGGTTTTTTTGGTTTCCCGGGTGAAAAGTGAATTTTGCCGTTTTGAGCCGGTGGTGTGTTTTTTGGGGGATCCg                                               >  1:2870897/1‑140 (MQ=255)
                                                                                    cTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAACGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGt                                             <  2:1738977/140‑1 (MQ=255)
                                                                                    cTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTGTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGt                                             <  2:365812/140‑1 (MQ=255)
                                                                                    cTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTGTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGt                                             <  1:3074754/140‑1 (MQ=255)
                                                                                        gaAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACTTTTTATTTTTTTTTGTTTGCCGGGTGAAAATTTAATTTTACCGTTTTGATCCGGTCTTCGGTTTTTTTGGCAATCACGTCGTg                                         >  1:511718/1‑140 (MQ=255)
                                                                                         aaGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCCATCCGGTCGTgg                                        <  2:2653043/140‑1 (MQ=255)
                                                                                         aaGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGAGTTTTTGTTTTTTTTGGTGTCCCGGGTGAAAAGTTAATTTTACCGTTTTGAGCCGTGGTTGTGTTTTTTTGGCAATACGGGCGTgg                                        >  1:2738336/1‑140 (MQ=255)
                                                                                            cagAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGGCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCAt                                     <  1:2657205/138‑1 (MQ=255)
                                                                                            cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCGATCCGGTCGTGGCAt                                     <  1:2380210/140‑1 (MQ=255)
                                                                                            cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTATTTTTTTTTCTGTACCGGGTGAAAAGTGAATTTTACCGTTTTCAGCCGTTCTTCTTTTTTTTTGGCAATACGGTCGTGGCAt                                     >  2:1520086/1‑140 (MQ=255)
                                                                                             tGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCAtt                                    <  1:2287326/140‑1 (MQ=255)
                                                                                              gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTTTGATTTTCTTTTTTTTTGTTTCCCGGGTGTAAAGTGTATTTTGCCCTTTTTATCCGGTGTTCTTTTTTTTTTGGAATCCGGTCGTGGGATTc                                   >  1:3160207/1‑140 (MQ=255)
                                                                                                 tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTAATTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                <  1:4014067/140‑1 (MQ=255)
                                                                                                 tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGCAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                <  2:448150/140‑1 (MQ=255)
                                                                                                  cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                               <  2:1586536/140‑1 (MQ=255)
                                                                                                  cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                               <  1:3505999/140‑1 (MQ=255)
                                                                                                  cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTCGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                               <  1:444967/140‑1 (MQ=255)
                                                                                                    ttttttgttttttgTATGGTAAAGCGGCCTCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                             <  1:2418935/140‑1 (MQ=255)
                                                                                                    ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                             <  2:1535904/140‑1 (MQ=255)
                                                                                                    ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                             <  2:329693/140‑1 (MQ=255)
                                                                                                    ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                             <  2:2815859/140‑1 (MQ=255)
                                                                                                    ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                             <  2:655911/140‑1 (MQ=255)
                                                                                                    ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                             <  2:2394935/140‑1 (MQ=255)
                                                                                                    ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                             <  2:2319656/140‑1 (MQ=255)
                                                                                                      ttttGTGTTTTGTTTGGTAAAGCGGGCGCTTTATTTTTTGACTTTTTGTTTTTTTTGCTGTCCCGGGTGTAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                           <  1:544166/140‑1 (MQ=255)
                                                                                                      ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATGCCTGGcaca                           <  1:3794157/140‑1 (MQ=255)
                                                                                                      ttttGTGTTGTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                           <  1:1001447/140‑1 (MQ=255)
                                                                                                      tcttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTGACTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                           <  2:3735742/138‑1 (MQ=255)
                                                                                                        ttGTGTTTTGTATGGTCACGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGGCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                         <  2:2896434/140‑1 (MQ=255)
                                                                                                        ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                         <  1:1238650/140‑1 (MQ=255)
                                                                                                        ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                         <  1:2614884/140‑1 (MQ=255)
                                                                                                        ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                         <  2:1849051/140‑1 (MQ=255)
                                                                                                        ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                         <  2:3004546/140‑1 (MQ=255)
                                                                                                        ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                         <  2:204469/140‑1 (MQ=255)
                                                                                                        ttGTGGTTTGTATGGTCAAGCCGCCGGTTTATTTTTTGACTTTTGCTTTTTTTTGCGGGCCCGGGTGAAAAGTGAATTGTCCCGTTGTCAGGCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                         <  2:2581071/140‑1 (MQ=255)
                                                                                                         tgtgTTTTGTATGGAAACGCGGCCGCTTTTTTTTTTGAGTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                        <  2:812542/140‑1 (MQ=255)
                                                                                                              tttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgta                   <  1:3564882/140‑1 (MQ=255)
                                                                                                               ttGTATGGTATACCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgtat                  <  2:2052045/140‑1 (MQ=255)
                                                                                                                            cGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTTCTGTACCCGGTTAAAAGTGAAATTTACCGTTTTAATCCGTTCTTCTGTTTTTTGGGGAATCCGGTAGTTGCATTACTGGCACAAATATGTATTGATTTGGCGGtt     >  2:22466/1‑140 (MQ=255)
                                                                                                                               ccGCTTTATTTTTTTACTTTTTGTTTTTTTTGCTGTCCCCGGTGAAAAGTGAATTTTCCCGTTTTAAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGGGGTTTCg  >  2:1429250/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                       
TCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCG  >  NZ_CP034943/1514723‑1514989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: