Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,743,439 G→T 5.7% L167L (CTG→CTT cotE → outer spore coat protein CotE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,743,4390GT 5.7% 498.3 / 12.5 158L167L (CTG→CTTcotEouter spore coat protein CotE
Reads supporting (aligned to +/- strand):  ref base G (56/93);  new base T (5/4);  total (61/97)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 159 total aligned reads displayed.

GAAATAAAATTGTTGTTCAGGCAGAAAGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGTTATAATGAAGGTTG  >  NZ_CP034943/1743303‑1743578
                                                                                                                                        |                                                                                                                                           
gAAATAAAATTGTTGTTCAGGCAGAAAGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTCGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGaa                                                                                                                                          <  2:1146370/140‑1 (MQ=255)
gAAATAAAATTGTTGTTCAGGCAGAAAGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGaa                                                                                                                                          <  2:1259591/140‑1 (MQ=255)
gAAATAAAATTGTTGTTCAGGCAGAAAGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGaa                                                                                                                                          <  2:1146358/140‑1 (MQ=255)
            ttgttCAGGCAGAATGGGAGTTTTTAGCGGAAGGGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAGGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCg                                                                                                                              >  1:3598154/1‑140 (MQ=255)
            ttgttCAGGCAGAACGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCg                                                                                                                              <  1:1000024/140‑1 (MQ=255)
            ttgttCAGGCAGAAAGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCg                                                                                                                              >  1:7673/1‑140 (MQ=255)
            ttgttCAGGCAGAAAGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCg                                                                                                                              <  1:1689538/140‑1 (MQ=255)
               ttCAGGCAGAAAGGGAGTTTTTAGCGGAAGTGGTAGGATAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGaa                                                                                                                           >  1:2034575/1‑140 (MQ=255)
                   ggCAGAAAGGGAGTTTTTAGCGGACGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAAtttt                                                                                                                       <  2:2764841/140‑1 (MQ=255)
                          aGGGAGTTTTTAGCGGTAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAgg                                                                                                                <  1:2379325/140‑1 (MQ=255)
                          aGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAgg                                                                                                                <  1:1024921/140‑1 (MQ=255)
                          aGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAgg                                                                                                                <  2:12090/140‑1 (MQ=255)
                          aGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAgg                                                                                                                <  1:524589/140‑1 (MQ=255)
                          aGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAgg                                                                                                                <  1:1652598/140‑1 (MQ=255)
                          aGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCGTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAgg                                                                                                                <  1:823658/140‑1 (MQ=255)
                                    tAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTgaa                                                                                                      <  1:2251248/140‑1 (MQ=255)
                                    tAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTgaa                                                                                                      <  1:1695526/140‑1 (MQ=255)
                                         gAAGTGGTAGGAGAAACAAAGGTAGGGGTTGAGTTCAATCCTGATGGGGAAGAGGACGACGAAGAAGATTGGGACGATGAGCTTGTTGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTCGGAGATCCTGAAGAATa                                                                                                 <  2:3682838/140‑1 (MQ=255)
                                              ggTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGACGACGAAGAAGCTGGGGAAGCTGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATaaaaaa                                                                                            <  1:210849/140‑1 (MQ=255)
                                              ggTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATaaaaaa                                                                                            <  2:3790598/140‑1 (MQ=255)
                                              ggTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATaaaaaa                                                                                            <  2:606603/140‑1 (MQ=255)
                                              ggTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATaaaaaa                                                                                            <  2:232182/140‑1 (MQ=255)
                                              ggTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATaaaaaa                                                                                            <  2:1715768/140‑1 (MQ=255)
                                              ggTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCGGGAAGACATCACCCCGGAATTTTTAGTAGGAGATCCTGAAGAATaaaaaa                                                                                            <  1:1293277/140‑1 (MQ=255)
                                               gTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAg                                                                                           >  2:16134/1‑140 (MQ=255)
                                                tAGGAGAAACAAAGGTCGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAgg                                                                                          <  1:354323/140‑1 (MQ=255)
                                                  ggAGAAACAACGGTAGTGGTTGAGGTAAATCCTGATTGGGAAGCGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGCACAGCTGGACGACATCAACCCGGAATTTTTAGGAGGAGATCCTGAAGAAGCAAAAAGGGa                                                                                        <  2:386187/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTCGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGATTAAAAAAGGGa                                                                                        <  2:385272/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAGGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGa                                                                                        <  2:3598154/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTCGGAGATCCTGAAGAATAAAAAAGGGa                                                                                        <  1:1607597/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGa                                                                                        <  2:1740946/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGa                                                                                        <  2:2077888/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGa                                                                                        <  2:2914106/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGa                                                                                        <  2:7673/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGa                                                                                        <  2:3604904/140‑1 (MQ=255)
                                                  ggAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGa                                                                                        <  2:373174/140‑1 (MQ=255)
                                                    agaAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACt                                                                                      <  2:2273021/140‑1 (MQ=255)
                                                     gaAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTa                                                                                     <  1:2460701/140‑1 (MQ=255)
                                                      aaaCAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAg                                                                                    >  2:741747/1‑140 (MQ=255)
                                                      aaaCAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAg                                                                                    >  2:2851869/1‑140 (MQ=255)
                                                           aaGGTCGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGTGAAGATGAGCTTGATGAAGAGCTGGAAGCCATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGgag                                                                               <  2:1970685/140‑1 (MQ=255)
                                                              gttgTGGTTGAGTTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACa                                                                            <  1:3039503/137‑1 (MQ=255)
                                                              gTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACa                                                                            <  1:427166/140‑1 (MQ=255)
                                                                aGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGt                                                                          <  1:2502347/140‑1 (MQ=255)
                                                                aGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGt                                                                          <  1:3813439/140‑1 (MQ=255)
                                                                 gttgTTGAGGTCAATCCTGACTGGGAAGAGGATGACGAGGAAGATTGGGAAGATGAGCTTGATGAAGAGCTTGAAGACATCAACCCGGAGTTTTTAGTGGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTa                                                                         >  2:415303/4‑140 (MQ=255)
                                                                 gTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTa                                                                         >  2:2973981/1‑140 (MQ=255)
                                                                       gAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCa                                                                   >  2:1124411/1‑140 (MQ=255)
                                                                        aGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCaa                                                                  >  1:1292892/1‑140 (MQ=255)
                                                                        aGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCaa                                                                  >  2:2778932/1‑140 (MQ=255)
                                                                        aGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCaa                                                                  >  2:1244606/1‑140 (MQ=255)
                                                                        aGGTCAATCCTGACTGGGAAGAGGATGACGAGGAAGATTGGGAAGATGAGCTTGATGAAGAGCTTGAAGACATCAACCCGGAGTTTTTAGTGGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCaa                                                                  >  1:2225945/1‑140 (MQ=255)
                                                                         ggTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAg                                                                 >  1:1765415/1‑140 (MQ=255)
                                                                         ggTCAATCCTGATGGGGAAGAGGAAGACGAAGACGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTGTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAg                                                                 >  2:1167636/1‑140 (MQ=255)
                                                                          gTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGt                                                                <  2:2584757/140‑1 (MQ=255)
                                                                          gTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGt                                                                <  2:642568/140‑1 (MQ=255)
                                                                            cAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGtct                                                              <  1:1960629/140‑1 (MQ=255)
                                                                            cAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGtct                                                              <  2:3221318/140‑1 (MQ=255)
                                                                            cAATCCTGAGTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGtct                                                              <  2:3223498/140‑1 (MQ=255)
                                                                            cAAGCCTGATTGGGACGAGGAAGACCAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGtct                                                              <  1:2826548/140‑1 (MQ=255)
                                                                             aaTCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGtctc                                                             >  1:758409/1‑140 (MQ=255)
                                                                              aTCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTctct                                                            >  2:840245/1‑140 (MQ=255)
                                                                              aTCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTctct                                                            >  2:2050207/1‑140 (MQ=255)
                                                                              aTCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTctct                                                            >  1:2490330/1‑140 (MQ=255)
                                                                               tCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAATCGACTAGGGGAGACAGTACACCCAAGTCTCtt                                                           >  2:2534642/1‑140 (MQ=255)
                                                                               tCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGt                                                                          <  2:3487140/125‑1 (MQ=255)
                                                                               tCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGt                                                                          >  1:3487140/1‑125 (MQ=255)
                                                                                 cTGATTGGGAAGAGGAAGACGAAGAAGATTGTGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCtttt                                                         <  2:1612199/140‑1 (MQ=255)
                                                                                    aTTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTCGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTta                                                      <  1:371143/140‑1 (MQ=255)
                                                                                    aTTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTta                                                      >  2:3073771/1‑140 (MQ=255)
                                                                                      tGGTAAGAGGACCACGAAGACGTTTGGGAAGCTCAGCTTGATGAACAGCTGCAAGACAGAAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTtata                                                    <  1:3304966/140‑1 (MQ=255)
                                                                                       gggAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTatat                                                   <  2:2024120/140‑1 (MQ=255)
                                                                                         gaagaggacgaCGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGAGTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTg                                                 >  1:657143/1‑140 (MQ=255)
                                                                                         gaagaggaagaCGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATAAACCCGGAATGTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTg                                                 >  1:1175/1‑140 (MQ=255)
                                                                                          aagaggaagaCGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGa                                                >  2:3028314/1‑140 (MQ=255)
                                                                                             aggaagaCGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAgg                                                                                   <  2:3146336/102‑1 (MQ=255)
                                                                                             aggaagaCGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAgg                                                                                   >  1:2521664/1‑102 (MQ=255)
                                                                                             aggaagaCGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAgg                                                                                   >  1:3146336/1‑102 (MQ=255)
                                                                                             aggaagaCGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAgg                                                                                   <  2:2521664/102‑1 (MQ=255)
                                                                                               gaagaCGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGTAGATCCTGAAGAATAAAAAAGGGACTAGGCGAGACAGTACCCCCAAGTCTCTTTTTTATATTGatatct                                           <  2:2390477/140‑3 (MQ=255)
                                                                                                    cGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTc                                      >  1:1568981/1‑140 (MQ=255)
                                                                                                      aagaatATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCtt                                    <  2:1556861/140‑1 (MQ=255)
                                                                                                          agaTTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTAt                                <  2:3084150/140‑1 (MQ=255)
                                                                                                                ggAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACttt                          >  1:1133978/1‑140 (MQ=255)
                                                                                                                ggAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACttt                          >  1:3068198/1‑140 (MQ=255)
                                                                                                                   aGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTg                       >  2:1501312/1‑140 (MQ=255)
                                                                                                                    cctGAGCTTGATTAAGATCTTTAAGACATCAACCCCGAATTTTTAGTAGGACATCCTGAAGAATAAAAAAGGGACTAGGGGAGACATTACCCCCAAGTCTCTTTTTTATATTGATATATAATTCTTTTATCACTTTTTGt                      <  1:3280087/138‑1 (MQ=255)
                                                                                                                      tGAGCTTGATGAAGAGCTTGAAGACATCAACCCGGAGTTTTTAGTGGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGttt                    >  2:3351221/1‑140 (MQ=255)
                                                                                                                      tGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGttt                    <  2:1252311/140‑1 (MQ=255)
                                                                                                                      tGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGttt                    >  2:1863330/1‑140 (MQ=255)
                                                                                                                         gCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATg                 <  2:3386380/140‑1 (MQ=255)
                                                                                                                          cTTGATGAAGAGCTTGAAGACATCAACCCGGAGTTTTTAGTGGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGt                >  1:3896691/1‑140 (MQ=255)
                                                                                                                           ttGATGAAGAGCTTGAAGACATCAACCCGGAGTTTTTAGTGGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGtt               <  1:3351221/140‑1 (MQ=255)
                                                                                                                           ttGATGAAGAGCTTGAAGACATCAACCCGGAGTTTTTAGTGGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGtt               <  1:3351780/140‑1 (MQ=255)
                                                                                                                           ttGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGtt               <  2:1428563/140‑1 (MQ=255)
                                                                                                                              atgaAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGTtata            >  1:967378/1‑140 (MQ=255)
                                                                                                                               tgaAGAGCTGGAAGACATCAACCCGGAATTTGTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCTCTTTTTGTTTATGTTCTaa           >  1:953616/1‑140 (MQ=255)
                                                                                                                                 aaGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGTTATAATg         <  2:1126010/140‑1 (MQ=255)
                                                                                                                                   gagCTTGAAGACATCAACCCGGAGTTTTTAGTGGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGTTATAATGaa       <  1:415303/140‑1 (MQ=255)
                                                                                                                                    agCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGTTATAATGAAg      >  1:2924438/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
GAAATAAAATTGTTGTTCAGGCAGAAAGGGAGTTTTTAGCGGAAGTGGTAGGAGAAACAAAGGTAGTGGTTGAGGTCAATCCTGATTGGGAAGAGGAAGACGAAGAAGATTGGGAAGATGAGCTTGATGAAGAGCTGGAAGACATCAACCCGGAATTTTTAGTAGGAGATCCTGAAGAATAAAAAAGGGACTAGGGGAGACAGTACCCCCAAGTCTCTTTTTTATATTGATATATCATTCTTTTATCACTTTTTGTTTATGTTATAATGAAGGTTG  >  NZ_CP034943/1743303‑1743578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: