Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,753,598 G→T 13.6% intergenic (+289/‑216) fabD → / → EO946_RS09090 ACP S‑malonyltransferase/acyltransferase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,753,5980GT13.6% 456.3 / 8.8 218intergenic (+289/‑216)fabD/EO946_RS09090ACP S‑malonyltransferase/acyltransferase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (69/109);  new base T (38/2);  total (107/111)
Fisher's exact test for biased strand distribution p-value = 1.09e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

CATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATT  >  NZ_CP034943/1753463‑1753735
                                                                                                                                       |                                                                                                                                         
cATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTTTGATACTGTAATTATTTtat                                                                                                                                       >  1:332295/1‑140 (MQ=255)
  tGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTatat                                                                                                                                     <  1:408806/140‑1 (MQ=255)
    tCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCATTATGTTatataa                                                                                                                                   >  1:3599712/1‑138 (MQ=255)
    tCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATTTTATATCa                                                                                                                                   >  1:917427/1‑140 (MQ=255)
    tCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTATTTGTTTATCCTGTCATTATGTTttataa                                                                                                                                   >  2:1763163/1‑138 (MQ=255)
        cacGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAAttt                                                                                                                               >  2:3161476/1‑140 (MQ=255)
           gATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTga                                                                                                                            <  2:354347/140‑1 (MQ=255)
              atTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCACTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGat                                                                                                                         <  2:1959022/140‑1 (MQ=255)
               tttGAACTATGTCAGTCAGCCGGAATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCTCTCCAAATTCTTTGAGTAGATTTTCTTTGTTGTTGCTTTATTTATGTTTTATCAATTTTgagttt                                                                                                                        <  2:713953/140‑4 (MQ=255)
                ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTGAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                                       <  1:1987342/140‑1 (MQ=255)
                ttGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCCTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGatat                                                                                                                       <  1:2941259/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAAct                                                                                                                  <  2:404562/140‑2 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  2:592226/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  1:1164845/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  1:3651888/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  2:461437/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  2:1286471/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  2:2124930/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  2:2498922/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  2:3460331/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  1:2212356/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCGTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTTTATCAATTTTGAGATATCAACa                                                                                                                  <  1:1763163/140‑1 (MQ=255)
                     cTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTGAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACa                                                                                                                  <  1:428015/140‑1 (MQ=255)
                     cTATCTCAGTCAGCCGGCAGATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTTTTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTACATCAATTTTGAGATATCAACa                                                                                                                  <  2:2267448/140‑1 (MQ=255)
                       aTGTCCGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGCGGTTCTTTGTTGCTCCTGTCATTATGTTATATGAAGTTTGAGATTTCAACATa                                                                                                                >  1:3185828/1‑140 (MQ=255)
                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTGATCCTGTCATTATGTTATATAAATTTTGAGATATAAAAATa                                                                                                                >  2:2652522/1‑140 (MQ=255)
                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACaaa                                                                                                                >  2:1395319/1‑138 (MQ=255)
                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCaaaaaa                                                                                                                >  1:1495941/1‑136 (MQ=255)
                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATa                                                                                                                >  2:2862117/1‑140 (MQ=255)
                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTAGTTTTTTTACAGTTCATTATTTTATATAAATTTTGACATATGAACATa                                                                                                                >  2:3243879/1‑140 (MQ=255)
                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTGATGCAGGCATTATGTTATTTCAATTTTGAGTTATGaaaaaa                                                                                                                >  1:1219322/1‑136 (MQ=255)
                       aTGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTGAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATa                                                                                                                >  1:3731874/1‑140 (MQ=255)
                        tGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATac                                                                                                               <  2:807498/140‑1 (MQ=255)
                        tGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATac                                                                                                               <  2:2554621/140‑1 (MQ=255)
                             gtcagCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTa                                                                                                          <  1:509170/140‑1 (MQ=255)
                             gtcagCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTATTTGTTGATCCTGTAATTATGTTATATGAATTTTTAGATATAAAAATAAAccaa                                                                                                          >  2:1473413/1‑138 (MQ=255)
                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGTCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:1149065/140‑1 (MQ=255)
                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:3315954/140‑1 (MQ=255)
                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:3740229/140‑1 (MQ=255)
                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  2:26096/140‑1 (MQ=255)
                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       <  1:1827806/140‑1 (MQ=255)
                                agCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGAc                                                                                                       >  1:2983845/1‑140 (MQ=255)
                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     <  2:2912225/140‑1 (MQ=255)
                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     <  2:358759/140‑1 (MQ=255)
                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACACAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     <  2:632999/140‑1 (MQ=255)
                                  ccGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCATTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCa                                                                                                     <  1:1349363/140‑1 (MQ=255)
                                   cGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGGTTGTTGTTCCTGTAAATATGTTATAACACTATTTGGATATAAACAAACACCTAGACCaa                                                                                                    >  1:3101538/1‑139 (MQ=255)
                                   cGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATACTGTCATTATGTTATATCAATTTTGAGATATCAACATAAACCTAGACCAc                                                                                                    >  1:3106228/1‑140 (MQ=255)
                                        tatCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGGTGTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCat                                                                                               >  2:517751/1‑140 (MQ=255)
                                            aTGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCATTATGTTATATCAATTTTGAGATATAAAAATAAAACAAGAACACTTCataata                                                                                           >  2:3958080/1‑140 (MQ=255)
                                            aTGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCataata                                                                                           <  1:1859202/140‑1 (MQ=255)
                                            aTGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCataata                                                                                           <  1:1319981/140‑1 (MQ=255)
                                               aTAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGTTCCTGTCATTATTTTATATCAATTTTTAGATATAAAAATACACATAGACCACTTCATAAAagat                                                                                        >  2:2649226/1‑139 (MQ=255)
                                                tAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGc                                                                                       <  2:2783407/140‑1 (MQ=255)
                                                tAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACACAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGc                                                                                       <  2:2384660/140‑1 (MQ=255)
                                                   aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTGATCCTGTGATTATGTTATATCAATTTTGAGATATAAAAATACACCTAGACCCCGTCATAATATAGCCGc                                                                                    >  1:3360794/1‑140 (MQ=255)
                                                   aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTTAAACTTTGATTATTTTATATGACTTTTGAGATTTAAACATAAACCGAGACCAATTGAAAATAGAGCCGc                                                                                    >  1:252771/1‑140 (MQ=255)
                                                   aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATCCTGTCATTATTTTATATGAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGc                                                                                    >  1:1169271/1‑140 (MQ=255)
                                                   aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATACTTTGATTATTTTATATAAATTTTGAGATATCAAAATAAACCGAGACCACTTCATAAAAAATCCGc                                                                                    >  1:258053/1‑140 (MQ=255)
                                                   aCCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGAGGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTTAGATATCAACATACACCTATACCACTTAATAATATATCCGc                                                                                    >  2:1819087/1‑140 (MQ=255)
                                                     cATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTCTTTTTTGATCCTGTCATTATGGTATATCAATTTTGAGATATCAACATACACCTAGACCACATAATAATAGACCCGCAt                                                                                  >  2:3690325/1‑140 (MQ=255)
                                                       tagCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACACAAATCTTAGATTCGATGTTCATTGTTGATGCTGTACATATTGTAAATAAATTTTGAGATATCAAAATACACCAACACCACTTCATAATAGACCCGCCTcc                                                                                >  2:3077652/3‑140 (MQ=255)
                                                       tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATCCGGTCATTATGTTATATGAATTTTGTGATATCAACATACACCTAGACCACTTCAGAATACAGCCGCATcc                                                                                >  2:1053363/1‑140 (MQ=255)
                                                       tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCATTATGTTATAACAATTTTGAGATAACAACATAAACCAATAACACATAATAATATAGCCGCAtac                                                                                >  2:1522390/1‑138 (MQ=255)
                                                       tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCGGTCATTATGTTATATCAATTTTGAGATATAAACATACACCAAGACCACATCATAATAGAGCCGCCTcc                                                                                >  2:1101461/1‑140 (MQ=255)
                                                       tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATGCTGTCATTATTTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCAGAATAGAGCCGCATcc                                                                                >  2:3854212/1‑140 (MQ=255)
                                                       tCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATACTGGCATTATGTTATATCAATTTTTAGATATCAACATACAACTATAACACATCATAATAGATCCCCaaac                                                                                >  2:1896930/1‑137 (MQ=255)
                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTGTTGATACATTCATTAATTTATATAAAATTTTAATTATAAAAAAACAACTAAAACACATCATAAAAAAGACGCATCCa                                                                               >  1:1609721/1‑140 (MQ=255)
                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTTTTCCTGTCATTATTTTATATCAATTTTGTGATATCAACAAACAACTAGACCACGTCATAATAGAGCCGCCTCCa                                                                               >  1:3404441/1‑140 (MQ=255)
                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCATTATGTTATATCAAATTTGAGATATCAACATAAACCTAGGACACGTCATAATAGAGCCGCCTccc                                                                               >  1:1046317/1‑139 (MQ=255)
                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATGCTGTCATTATTTTATATCAATTTTTATATATCAACATACACCTAGACCACTTCATAAAAGATCCGCATACa                                                                               >  2:2416898/1‑140 (MQ=255)
                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTGGTTGTTGTTGCTGTGATTATGTTTTATGAATTTTTTGATATAAAAATAAACCTAGACCACGTCCTAATAGAGCCGccacca                                                                               >  1:3529325/1‑135 (MQ=255)
                                                        cGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGGTCTTTGTTGATCCTGTCATTATTTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGcc                                                                                      >  1:2652406/1‑133 (MQ=255)
                                                        cGCTCTCAACTCAAGATGGCCGTCTATTTAACCGCACCCAAAGCTTAGAGTCGATTTTCGTTGTTGTTACTGGGATTATTTTATATGAATGTTGATGTATCAACATACAACTATGCCACTTCATACTATATCCGGATCCa                                                                               >  2:1606941/1‑140 (MQ=255)
                                                               aactcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCCCGTCAGAATAGAGCCGCCTCCAGAGCAcc                                                                        >  1:3276502/1‑140 (MQ=255)
                                                               aactcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATGCTGTGATTATTTTATATGAATTTTGTGCTATCAACATACACCTATACCACGTCATAATAGTTCCGCATCCAGAGCAcc                                                                        >  1:2613097/1‑140 (MQ=255)
                                                                 ctcaaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCaa                                                                      <  1:642063/140‑1 (MQ=255)
                                                                    aaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTGTTTTTTTATCCTTTCCTTTTGTTTTATGAATTTTTATATATAAACATAAAACAAGAACACATAATAATAGAGCCGCATACCGAGCACCaaaaa                                                                   >  1:1480434/1‑139 (MQ=255)
                                                                    aaGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTAATTGTTGATCCTGTCATTATGTTATATCAATATTGAGATATCAAAATACAACGAGACCACATCATAAAAGATCCGCATCCAGAGCACCAAAAg                                                                   >  2:1758043/1‑140 (MQ=255)
                                                                           ccTTCTATTTAACCGCACCCAAATCTGAGAGTCGATGTTCTTTGTTGTTCCTTTCATTATGTTATATCAATTTTGATATATCAACATACAACTAGAACACTTCATAATAGAGCCGCATCCAGAGAACCAAAAGCAAtcct                                                            >  2:871167/1‑140 (MQ=255)
                                                                                tATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTTTTTATCCAGTCAATATTTTATATAAAATTTTAGATATAAAAAAAAAACTAGACCACTTCATAATAGATCCCCATACAGAGCACCAAAAGCAATCCTCCTAt                                                       >  1:1420093/1‑140 (MQ=255)
                                                                                     aaCCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATAAACCTATACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGtt                                                  >  2:4003407/1‑140 (MQ=255)
                                                                                     aaCCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTTATCCTGTCATTAAGTTAGATCCATTTTGAGATAAAAACATAAACCGAGACCACGTCATAATAGTGCCGCATACATAGAAACAAAAGAAATACTACGATTAGtt                                                  >  2:825717/1‑140 (MQ=255)
                                                                                          cACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGGTATATAAAATTTGAGATATCAACATACACCAAGACCACTTCCTAATAGAGCCGCATACCGAGCACCAAAAGCAATCCTCCGATTATGTGAttg                                             >  2:783160/1‑139 (MQ=255)
                                                                                             ccAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGAttcttc                                          <  2:2952624/140‑1 (MQ=255)
                                                                                             ccAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAACCAATCCTCCTATTAGTTGAttcttc                                          <  2:1392689/140‑1 (MQ=255)
                                                                                                     tAGAGTCGATGTTCTTTGTTGATCCTGTCATTATTTTATATCAATTTTGAGATATCAACATACAACTAGACCAATTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAttttt                                  <  1:3989365/140‑1 (MQ=255)
                                                                                                     tAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAttttt                                  <  1:1232705/140‑1 (MQ=255)
                                                                                                     tAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAttttt                                  <  1:768775/140‑1 (MQ=255)
                                                                                                      agagTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAtttttt                                 <  2:1223723/140‑1 (MQ=255)
                                                                                                      agagTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTAtttttt                                 <  2:3236387/140‑1 (MQ=255)
                                                                                                             aTGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTAt                          <  2:418392/140‑1 (MQ=255)
                                                                                                                    ttgttgATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCtttt                   <  1:2662528/140‑1 (MQ=255)
                                                                                                                         gATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAAtt              <  1:2351319/140‑1 (MQ=255)
                                                                                                                         gATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTGTTCAAtt              <  2:3911170/140‑1 (MQ=255)
                                                                                                                          aTCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTa             >  2:1311184/1‑140 (MQ=255)
                                                                                                                               gTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat        <  1:3837307/140‑1 (MQ=255)
                                                                                                                               gTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat        <  1:1522390/140‑1 (MQ=255)
                                                                                                                               gTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAatat        <  2:3818969/140‑1 (MQ=255)
                                                                                                                                   ttATGTTAGATCAAATTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATcaca    <  2:2054270/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
CATGTCCACACGATATTTGAACTATGTCAGTCAGCCGGCATATCATGATAGACCATCGCTCTCAACTCAAGATGGCCTTCTATTTAACCGCACCCAAATCTTAGAGTCGATGTTCTTTGTTGATCCTGTCATTATGTTATATCAATTTTGAGATATCAACATACACCTAGACCACTTCATAATAGAGCCGCATCCAGAGCACCAAAAGCAATCCTCCTATTAGTTGATTCTTCTTATTTTTTACATTATGTCTTTTCAATTAAATATCACATT  >  NZ_CP034943/1753463‑1753735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: