Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,805,539 G→A 6.2% T773T (ACG→ACA EO946_RS09140 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,805,5390GA 6.2% 627.8 / 21.0 192T773T (ACG→ACAEO946_RS09140non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (97/83);  new base A (5/7);  total (102/90)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 192 total aligned reads displayed.

TTCCCTAAAGAATTAGTGAAAAAGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGG  >  NZ_CP034943/1805402‑1805673
                                                                                                                                         |                                                                                                                                      
ttCCCTAAAGAATTAGTGAAAAAGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGcc                                                                                                                                      >  2:1501738/1‑140 (MQ=255)
           aTTAGTGAAAAAGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGc                                                                                                                           >  2:3139953/1‑140 (MQ=255)
             tAGTGAAAAAGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCt                                                                                                                         >  2:691795/1‑140 (MQ=255)
              aGTGAAAAAGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTg                                                                                                                        >  1:1538900/1‑140 (MQ=255)
                    aaaGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCa                                                                                                                  >  2:1635512/1‑140 (MQ=255)
                       ggCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAATTCACCATACGCCGATTGGAAAGCCTGTCTCCAACa                                                                                                               >  2:1700981/1‑140 (MQ=255)
                         cgttATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACTGACCTAGTGAAGCCTCCATTTACGCTGCGTATTTAGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGGCGATTGGAAAGCCTGTCTCCAACATg                                                                                                             <  2:2060530/137‑1 (MQ=255)
                         cGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                             <  1:1025039/140‑1 (MQ=255)
                         cGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                             <  2:3524829/140‑1 (MQ=255)
                                gATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAAttt                                                                                                      <  1:1501738/140‑1 (MQ=255)
                                      tAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAATGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATtg                                                                                                >  1:1962550/1‑140 (MQ=255)
                                      tAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATtg                                                                                                >  1:3080841/1‑140 (MQ=255)
                                              gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                        <  1:103020/140‑1 (MQ=255)
                                              gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                        >  2:1122461/1‑140 (MQ=255)
                                              gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                        <  1:234893/140‑1 (MQ=255)
                                              gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                        <  1:1619685/140‑1 (MQ=255)
                                               ccGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGc                                                                                       <  1:689018/140‑1 (MQ=255)
                                               ccGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGc                                                                                       <  1:1293954/140‑1 (MQ=255)
                                                     tGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGa                                                                                 <  1:63574/140‑1 (MQ=255)
                                                      gAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGaa                                                                                <  2:2555982/140‑1 (MQ=255)
                                                              ttACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTgtgt                                                                        >  2:1968615/1‑140 (MQ=255)
                                                                aCGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCg                                                                      <  2:1152252/140‑1 (MQ=255)
                                                                      cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                <  2:1903887/140‑1 (MQ=255)
                                                                       aaCTTAAGCCTCCATTTAATATTATTATTTCGGATGATGAAAAGGAGATATTGCAAGTCACCATACGCCGATTTGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaa                                                               >  2:2644994/2‑140 (MQ=255)
                                                                       aaCTGAAGCCTCCATATACGCTTAATATTACGGATTAGGAAAAGGAGATAATGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaa                                                               >  2:3227444/2‑140 (MQ=255)
                                                                        acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                              >  1:646326/4‑140 (MQ=255)
                                                                        acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                              >  1:3490459/4‑140 (MQ=255)
                                                                        aCTGAAGCCTCCATTTACGCTGCTTATTTCGGATGCGGAAAAGGAGATAATGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                              <  2:2487941/140‑1 (MQ=255)
                                                                        aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                              >  2:3223860/1‑140 (MQ=255)
                                                                              gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                             <  1:3228679/87‑1 (MQ=255)
                                                                              gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTgg                                                        >  1:1334070/1‑140 (MQ=255)
                                                                               ccTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGt                                                       <  2:1923608/140‑1 (MQ=255)
                                                                               ccTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCAACATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTTTCGATCGGAAAGCCTGGt                                                       <  2:3618669/140‑1 (MQ=255)
                                                                                cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                      >  1:249629/1‑140 (MQ=255)
                                                                                cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                      >  1:800151/1‑140 (MQ=255)
                                                                                cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                      >  1:3629566/1‑140 (MQ=255)
                                                                                cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                      >  1:732709/1‑140 (MQ=255)
                                                                                 tCCATTTACGCTGCGTATTTCGGATGCGGAACAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGACAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGa                                                     >  2:2186944/1‑140 (MQ=255)
                                                                                 tCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGa                                                     >  1:3905327/1‑140 (MQ=255)
                                                                                  ccATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAg                                                    <  1:1646900/140‑1 (MQ=255)
                                                                                   cATTTACGCTGCGTATTTCGGATGCGGAAAAGGCGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                   >  1:1367901/1‑140 (MQ=255)
                                                                                   cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGc                                                   >  1:2152520/1‑140 (MQ=255)
                                                                                   cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                   >  1:2535742/1‑140 (MQ=255)
                                                                                   cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                   >  1:2246017/1‑140 (MQ=255)
                                                                                   cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                   >  1:2121594/1‑140 (MQ=255)
                                                                                   cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCGGGTGAGc                                                   >  1:2236208/1‑140 (MQ=255)
                                                                                        aCGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGc                                              >  2:3242986/1‑140 (MQ=255)
                                                                                        aCGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGc                                              >  2:1839169/1‑140 (MQ=255)
                                                                                           cTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATc                                           >  1:3505912/1‑140 (MQ=255)
                                                                                             gCGTATTTCGGATGCGGAAAAGGATATATTGCAAGTCACCATACGCCGATTGGAAAGACTGTCTCCAACATGAAAATTTATATTGTGGACAAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGAATctc                                         >  2:1056874/1‑138 (MQ=255)
                                                                                             gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                        >  2:249740/1‑93 (MQ=255)
                                                                                             gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                         >  1:1730276/1‑140 (MQ=255)
                                                                                             gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                         >  1:346070/1‑140 (MQ=255)
                                                                                             gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                         >  1:4003344/1‑140 (MQ=255)
                                                                                             gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCGGGTGAGCTTTGCATCGc                                         >  2:1092304/1‑140 (MQ=255)
                                                                                              cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGCTTTGCATCGCa                                        >  1:3029021/1‑140 (MQ=255)
                                                                                              cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                        >  2:519038/1‑140 (MQ=255)
                                                                                              cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                        >  2:2654774/1‑140 (MQ=255)
                                                                                               gTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAACTTTAGATTGTGGACCAGCTTTTGTAACCTGGGTCGATCGGACAGCGGGGTGAGCTTTGCATCGcgg                                       >  1:2169115/1‑138 (MQ=255)
                                                                                                  tttCTGATGCGGAAAAGGAGATATTGCAAGTGACCCTCCGCCGCTTGGAAAGCATGTCTCCAAGATCAAAATTTATCTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGCTTTGAATCGCATGCg                                    <  1:1700981/140‑1 (MQ=255)
                                                                                                    tCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGAc                                  <  2:624795/140‑1 (MQ=255)
                                                                                                    tCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGAc                                  <  2:1650444/140‑1 (MQ=255)
                                                                                                       gATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGt                               <  2:3080841/140‑1 (MQ=255)
                                                                                                       gATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGt                               <  1:2889900/140‑1 (MQ=255)
                                                                                                        aTGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGtt                              >  2:3929665/1‑140 (MQ=255)
                                                                                                        aTGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGtt                              >  1:3804024/1‑140 (MQ=255)
                                                                                                        aTGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGtt                              >  1:442932/1‑140 (MQ=255)
                                                                                                            ggAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGc                          <  2:2356621/140‑1 (MQ=255)
                                                                                                              aaaaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCaa                        <  1:1122461/140‑1 (MQ=255)
                                                                                                                aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                      <  1:3790424/140‑1 (MQ=255)
                                                                                                                aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                      <  2:1025196/140‑1 (MQ=255)
                                                                                                                aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                      <  1:1459532/140‑1 (MQ=255)
                                                                                                                aaGGAGATATTGCAAGGCACCATACGCCGATTGGAAAGCCTGGCTCCAACATGAAAATTTATATGGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                      <  2:2764855/140‑1 (MQ=255)
                                                                                                                aaGGAGATATTGCAACTCCCCACACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATGGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                      <  2:1376225/140‑1 (MQ=255)
                                                                                                                   gagagaTCGCAAGTCACCATACACAGATTGGAAAGCCTGTTTCCAACACGAAAATTGATATTGTGGACCAGCATTTGAAGCCGGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAggc                   <  1:182576/140‑2 (MQ=255)
                                                                                                                   gagaTATTGCAATTCACCAGACGCCGAGTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                   <  2:3413135/140‑1 (MQ=255)
                                                                                                                   gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTTCATCGCAGGCGACGGTTTGGCAAGAGGa                   >  2:1764737/1‑140 (MQ=255)
                                                                                                                   gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                   >  2:2622602/1‑140 (MQ=255)
                                                                                                                     tatatTGCAAGTCACCATACGCAGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  1:3943546/139‑1 (MQ=255)
                                                                                                                     gagcggccAAGTCCCCCTACGCCGATTGGAAAGCCTGTCTCCAACCCGAACATTTATCCGGAGGCCCAGCCTTTCAAACCAGGCTCGATCGGAAACCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  1:519038/133‑1 (MQ=255)
                                                                                                                     gagatTGCAACTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  1:2719380/137‑1 (MQ=255)
                                                                                                                     gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  1:2654774/140‑1 (MQ=255)
                                                                                                                     gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  2:3983107/140‑1 (MQ=255)
                                                                                                                     gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  1:2013188/140‑1 (MQ=255)
                                                                                                                     gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  2:2027169/140‑1 (MQ=255)
                                                                                                                     gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  1:3139953/140‑1 (MQ=255)
                                                                                                                     gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                 <  2:1332769/140‑1 (MQ=255)
                                                                                                                        atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTCTATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta              >  1:116474/1‑140 (MQ=255)
                                                                                                                        atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                   <  2:1530543/135‑1 (MQ=255)
                                                                                                                        atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta              >  1:293482/1‑140 (MQ=255)
                                                                                                                        atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta              >  2:3661245/1‑140 (MQ=255)
                                                                                                                        atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta              >  2:1675813/1‑140 (MQ=255)
                                                                                                                        atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta              >  2:1413585/1‑140 (MQ=255)
                                                                                                                            cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATCCTataat          >  2:450576/1‑140 (MQ=255)
                                                                                                                            cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATAGTGTGGACCAGCATTTGAAACCTGTGTCGATCGGCAAGCCGGGTGAGGTTTGCATCGCAGGCGACGGTTTGGCACGAGGAGACTctaat          >  2:128353/1‑140 (MQ=255)
                                                                                                                            cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATGTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTataat          >  2:3880523/1‑140 (MQ=255)
                                                                                                                                  aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg    <  2:2930419/140‑1 (MQ=255)
                                                                                                                                  aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg    <  2:646326/140‑1 (MQ=255)
                                                                                                                                  aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg    <  2:3491994/140‑1 (MQ=255)
                                                                                                                                    cATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAAAAAGCCggg  >  2:874537/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TTCCCTAAAGAATTAGTGAAAAAGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGG  >  NZ_CP034943/1805402‑1805673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: