Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,805,557 C→T 5.7% V779V (GTC→GTT)  EO946_RS09140 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,805,5570CT 5.7% 697.6 / 20.7 209V779V (GTC→GTT) EO946_RS09140non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (104/93);  new base T (5/7);  total (109/100)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 216 total aligned reads displayed.

AAAAGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATA  >  NZ_CP034943/1805421‑1805695
                                                                                                                                        |                                                                                                                                          
    ggCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAATTCACCATACGCCGATTGGAAAGCCTGTCTCCAACa                                                                                                                                     >  2:1700981/1‑140 (MQ=255)
    ggCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACa                                                                                                                                     >  2:1665931/1‑140 (MQ=255)
      cGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                                                   <  2:3524829/140‑1 (MQ=255)
      cGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                                                   <  2:1524805/140‑1 (MQ=255)
      cGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                                                   <  2:1596442/140‑1 (MQ=255)
      cGGTATCGATCTTTAAAAACCGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCAGACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                                                   <  2:1103171/140‑1 (MQ=255)
             gATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAAttt                                                                                                                            <  2:1139740/140‑1 (MQ=255)
                   tAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATtg                                                                                                                      >  1:3080841/1‑140 (MQ=255)
                           gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                              <  1:103020/140‑1 (MQ=255)
                           gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                              <  1:1619675/140‑1 (MQ=255)
                           gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                              <  2:312895/140‑1 (MQ=255)
                           gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                              >  2:213223/1‑140 (MQ=255)
                            ccGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGc                                                                                                             <  1:689018/140‑1 (MQ=255)
                            ccGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGc                                                                                                             <  1:1293954/140‑1 (MQ=255)
                                  tGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGa                                                                                                       <  1:63574/140‑1 (MQ=255)
                                  tGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGa                                                                                                       <  1:2167292/140‑1 (MQ=255)
                                   gAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGaa                                                                                                      <  2:2555982/140‑1 (MQ=255)
                                   gAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGaa                                                                                                      <  2:1917779/140‑1 (MQ=255)
                                     aaaTATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAAc                                                                                                    <  1:1464727/140‑1 (MQ=255)
                                     aaaTATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAAc                                                                                                    <  1:1635512/140‑1 (MQ=255)
                                                   cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                      <  1:213223/140‑1 (MQ=255)
                                                   cTACTGAAGCCTCCATTTACGCTGCGTAGTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATCTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                      >  1:2103542/1‑140 (MQ=255)
                                                     acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                    >  1:646326/4‑140 (MQ=255)
                                                     acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                    >  1:3490459/4‑140 (MQ=255)
                                                     acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                    >  1:2930419/4‑140 (MQ=255)
                                                     aCTGAAGCCTCCATTTACGCTGCTTATTTCGGATGCGGAAAAGGAGATAATGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                    <  2:2487941/140‑1 (MQ=255)
                                                     aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaca                                                                                    <  2:1974024/140‑3 (MQ=255)
                                                     aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                    >  2:3224222/1‑140 (MQ=255)
                                                     aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                    <  2:2642757/140‑1 (MQ=255)
                                                           gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                                                   >  2:3228679/1‑87 (MQ=255)
                                                           gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTgg                                                                              >  1:1972831/1‑140 (MQ=255)
                                                           gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTgg                                                                              >  1:1334070/1‑140 (MQ=255)
                                                            gcTCCATTTACTCTGCGTATTTCTGTTGCTGAAAAGGATATATTGGAAGTCACAATACGCCGTTTGGAAAGGCTGTCTCCAACATGCATATTTATATTGTGGACCAGCATTTGAAACCTGTGTCTATCGGAAAGGCTGGt                                                                             <  2:3362805/139‑1 (MQ=255)
                                                            ccTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCAACATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTTTCGATCGGAAAGCCTGGt                                                                             <  2:3618669/140‑1 (MQ=255)
                                                             cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                            >  1:800151/1‑140 (MQ=255)
                                                             cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                            >  1:732709/1‑140 (MQ=255)
                                                             cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                            >  1:249629/1‑140 (MQ=255)
                                                              tCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGa                                                                           >  2:3942768/1‑140 (MQ=255)
                                                               ccATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAg                                                                          <  2:72615/140‑1 (MQ=255)
                                                                cATTTACGCTGCGTATTTCGGATGCGGAAAAGGCGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                         >  1:1367901/1‑140 (MQ=255)
                                                                cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                         >  1:2246017/1‑140 (MQ=255)
                                                                cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                         >  1:391728/1‑140 (MQ=255)
                                                                cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                         >  1:398452/1‑140 (MQ=255)
                                                                cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                         >  1:3615667/1‑140 (MQ=255)
                                                                cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                         >  1:3195766/1‑140 (MQ=255)
                                                                cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                         >  1:2535742/1‑140 (MQ=255)
                                                                cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCGGGTGAGc                                                                         >  1:2236208/1‑140 (MQ=255)
                                                                     aCGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGc                                                                    >  2:1839169/1‑140 (MQ=255)
                                                                        cTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATc                                                                 >  1:3505912/1‑140 (MQ=255)
                                                                          gCGTATTTCGGATGCGGAAAAGGATATATTGCAAGTCACCATACGCCGATTGGAAAGACTGTCTCCAACATGAAAATTTATATTGTGGACAAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGAATctc                                                               >  2:1056874/1‑138 (MQ=255)
                                                                          gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                              >  2:249740/1‑93 (MQ=255)
                                                                          gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                               >  1:243477/1‑140 (MQ=255)
                                                                          gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                               >  1:346070/1‑140 (MQ=255)
                                                                           cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGCTTTGCATCGCa                                                              >  1:3029021/1‑140 (MQ=255)
                                                                           cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGt                                                                             >  2:1191690/1‑125 (MQ=255)
                                                                           cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                                              >  2:519038/1‑140 (MQ=255)
                                                                           cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                                              >  2:2654774/1‑140 (MQ=255)
                                                                               tttCTGATGCGGAAAAGGAGATATTGCAAGTGACCCTCCGCCGCTTGGAAAGCATGTCTCCAAGATCAAAATTTATCTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGCTTTGAATCGCATGCg                                                          <  1:1700981/140‑1 (MQ=255)
                                                                               tttCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCg                                                          <  1:1665931/140‑1 (MQ=255)
                                                                                 tCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGAc                                                        <  2:1650444/140‑1 (MQ=255)
                                                                                 tCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGAc                                                        <  2:624795/140‑1 (MQ=255)
                                                                                    gATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGt                                                     <  1:2889900/140‑1 (MQ=255)
                                                                                           aaaaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCaa                                              <  1:1122461/140‑1 (MQ=255)
                                                                                             aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                            <  2:1025196/140‑1 (MQ=255)
                                                                                             aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                            <  2:1957052/140‑1 (MQ=255)
                                                                                             aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                            <  1:3790424/140‑1 (MQ=255)
                                                                                             aaGGAGATATTGCAAGGCACCATACGCCGATTGGAAAGCCTGGCTCCAACATGAAAATTTATATGGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                            <  2:2764855/140‑1 (MQ=255)
                                                                                             aaGGAGATATTGCAACTCCCCACACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATGGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                            <  2:1376225/140‑1 (MQ=255)
                                                                                                gagagaTCGCAAGTCACCATACACAGATTGGAAAGCCTGTTTCCAACACGAAAATTGATATTGTGGACCAGCATTTGAAGCCGGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAggc                                         <  1:182576/140‑2 (MQ=255)
                                                                                                gagaTATTGCAATTCACCAGACGCCGAGTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                         <  2:3413135/140‑1 (MQ=255)
                                                                                                gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTTCATCGCAGGCGACGGTTTGGCAAGAGGa                                         >  2:1764737/1‑140 (MQ=255)
                                                                                                  gagcggccAAGTCCCCCTACGCCGATTGGAAAGCCTGTCTCCAACCCGAACATTTATCCGGAGGCCCAGCCTTTCAAACCAGGCTCGATCGGAAACCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                       <  1:519038/133‑1 (MQ=255)
                                                                                                  gagatTGCAACTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                       <  1:2719380/137‑1 (MQ=255)
                                                                                                  gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                       <  1:3876199/140‑1 (MQ=255)
                                                                                                  gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                       <  1:1928916/140‑1 (MQ=255)
                                                                                                  gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                       <  1:4018405/140‑1 (MQ=255)
                                                                                                  gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                       <  2:1332769/140‑1 (MQ=255)
                                                                                                     atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTCTATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                    >  1:116474/1‑140 (MQ=255)
                                                                                                     atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                         >  1:1530543/1‑135 (MQ=255)
                                                                                                     atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                    >  1:2935082/1‑140 (MQ=255)
                                                                                                     atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                    >  2:1413585/1‑140 (MQ=255)
                                                                                                         cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATCCTataat                                >  2:450576/1‑140 (MQ=255)
                                                                                                         cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATGTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTataat                                >  2:3880523/1‑140 (MQ=255)
                                                                                                            gTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAACATTTATATTGTGGACCAGCATTTGAACCCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAg                             <  2:2041897/140‑1 (MQ=255)
                                                                                                               aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                          <  2:646326/140‑1 (MQ=255)
                                                                                                               aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                          <  2:2930419/140‑1 (MQ=255)
                                                                                                                          aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc               >  1:1020413/1‑140 (MQ=255)
                                                                                                                          aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc               >  2:3222199/1‑140 (MQ=255)
                                                                                                                           ttGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCt              >  2:1408512/1‑140 (MQ=255)
                                                                                                                             ggAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGa            <  2:1739237/140‑1 (MQ=255)
                                                                                                                              gacaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaa           <  2:3654797/137‑1 (MQ=255)
                                                                                                                               aaaGCCTGTCTCCAACATGACAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCGTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTCTAATAAGCCGGGTCTTACGGCTGaaa          <  2:3717814/140‑1 (MQ=255)
                                                                                                                               aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa          <  1:3242986/140‑1 (MQ=255)
                                                                                                                               aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa          <  2:1157890/140‑1 (MQ=255)
                                                                                                                               aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa          <  2:2209097/140‑1 (MQ=255)
                                                                                                                                     tGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTta    >  2:3608928/1‑140 (MQ=255)
                                                                                                                                      gTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtat   >  1:935337/1‑140 (MQ=255)
                                                                                                                                      gTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtat   >  1:2926832/1‑140 (MQ=255)
                                                                                                                                       tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata  <  1:3661245/140‑1 (MQ=255)
                                                                                                                                       tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata  <  1:2325627/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
AAAAGGCGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATA  >  NZ_CP034943/1805421‑1805695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑