Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,805,565 T→C 6.1% M782T (ATG→ACG)  EO946_RS09140 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,805,5650TC 6.1% 690.0 / 22.4 211M782T (ATG→ACG) EO946_RS09140non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (101/97);  new base C (5/8);  total (106/105)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 211 total aligned reads displayed.

CGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAAC  >  NZ_CP034943/1805427‑1805701
                                                                                                                                          |                                                                                                                                        
cGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                                                         <  2:1596442/140‑1 (MQ=255)
cGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                                                         <  2:1524805/140‑1 (MQ=255)
       gATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAAttt                                                                                                                                  <  1:1501738/140‑1 (MQ=255)
       gATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAAttt                                                                                                                                  <  2:1139740/140‑1 (MQ=255)
             tAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAATGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATtg                                                                                                                            >  1:1962550/1‑140 (MQ=255)
             tAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATtg                                                                                                                            >  1:3085219/1‑140 (MQ=255)
                     gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                                    <  1:103020/140‑1 (MQ=255)
                     gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                                    <  1:234893/140‑1 (MQ=255)
                     gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                                    >  2:213223/1‑140 (MQ=255)
                     gCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                                    <  2:312895/140‑1 (MQ=255)
                      ccGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGc                                                                                                                   <  1:689018/140‑1 (MQ=255)
                      ccGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGc                                                                                                                   <  1:1293954/140‑1 (MQ=255)
                            tGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGa                                                                                                             <  1:63574/140‑1 (MQ=255)
                             gAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGaa                                                                                                            <  2:2555982/140‑1 (MQ=255)
                               aaaTATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAAc                                                                                                          <  1:1635512/140‑1 (MQ=255)
                                     ttACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTgtgt                                                                                                    >  2:1323551/1‑140 (MQ=255)
                                     ttACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTgtgt                                                                                                    >  2:1968615/1‑140 (MQ=255)
                                             cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                            <  2:1903887/140‑1 (MQ=255)
                                             cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                            >  1:469398/1‑140 (MQ=255)
                                               acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                          >  1:646326/4‑140 (MQ=255)
                                               acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                          >  1:3490459/4‑140 (MQ=255)
                                               aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                          <  2:1792076/140‑1 (MQ=255)
                                               aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                          <  2:2642757/140‑1 (MQ=255)
                                               aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                          >  2:3224222/1‑140 (MQ=255)
                                               aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                          >  2:51583/1‑140 (MQ=255)
                                               aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                          >  2:534542/1‑140 (MQ=255)
                                                     gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATg                                                                                                                                         >  2:3228679/1‑87 (MQ=255)
                                                     gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTgg                                                                                    >  1:1334070/1‑140 (MQ=255)
                                                      gcTCCATTTACTCTGCGTATTTCTGTTGCTGAAAAGGATATATTGGAAGTCACAATACGCCGTTTGGAAAGGCTGTCTCCAACATGCATATTTATATTGTGGACCAGCATTTGAAACCTGTGTCTATCGGAAAGGCTGGt                                                                                   <  2:3362805/139‑1 (MQ=255)
                                                      ccTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGt                                                                                   <  2:1923608/140‑1 (MQ=255)
                                                       cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTg                                                                                  >  2:1362836/1‑140 (MQ=255)
                                                       cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                  >  1:732709/1‑140 (MQ=255)
                                                       cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                  >  1:3629566/1‑140 (MQ=255)
                                                       cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                  >  1:800151/1‑140 (MQ=255)
                                                         ccATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAg                                                                                <  2:72615/140‑1 (MQ=255)
                                                          cATTTACGCTGCGTATTTCGGATGCGGAAAAGGCGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                               >  1:1367901/1‑140 (MQ=255)
                                                          cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGc                                                                               >  1:2152520/1‑140 (MQ=255)
                                                          cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                               >  1:3195766/1‑140 (MQ=255)
                                                          cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                               >  1:2121594/1‑140 (MQ=255)
                                                          cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                               >  1:391728/1‑140 (MQ=255)
                                                          cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                               >  1:398452/1‑140 (MQ=255)
                                                              tACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTg                                                                           <  1:223658/140‑1 (MQ=255)
                                                               aCGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGc                                                                          >  2:1839169/1‑140 (MQ=255)
                                                                    gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAg                                                                                                                    <  1:249740/93‑1 (MQ=255)
                                                                    gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                                     >  1:2209097/1‑140 (MQ=255)
                                                                    gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                                     >  1:346070/1‑140 (MQ=255)
                                                                    gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCGGGTGAGCTTTGCATCGc                                                                     >  2:1092304/1‑140 (MQ=255)
                                                                     cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGaaa                                                                                                           <  1:3057803/101‑1 (MQ=255)
                                                                     cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGt                                                                                   >  2:1191690/1‑125 (MQ=255)
                                                                     cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                                                    >  1:2640318/1‑140 (MQ=255)
                                                                     cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                                                    >  2:2654774/1‑140 (MQ=255)
                                                                         tttCTGATGCGGAAAAGGAGATATTGCAAGTGACCCTCCGCCGCTTGGAAAGCATGTCTCCAAGATCAAAATTTATCTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGCTTTGAATCGCATGCg                                                                <  1:1700981/140‑1 (MQ=255)
                                                                         tttCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCg                                                                <  1:1665931/140‑1 (MQ=255)
                                                                              gATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGt                                                           <  1:2889900/140‑1 (MQ=255)
                                                                               aTGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGtt                                                          >  2:3929665/1‑140 (MQ=255)
                                                                                   ggAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGc                                                      <  2:1030593/140‑1 (MQ=255)
                                                                                    gAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCa                                                     >  1:231900/1‑140 (MQ=255)
                                                                                     aaaaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCaa                                                    <  1:1122461/140‑1 (MQ=255)
                                                                                       aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                  <  1:3790424/140‑1 (MQ=255)
                                                                                       aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                  <  1:1459532/140‑1 (MQ=255)
                                                                                       aaGGAGATATTGCAAGGCACCATACGCCGATTGGAAAGCCTGGCTCCAACATGAAAATTTATATGGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                  <  2:2764855/140‑1 (MQ=255)
                                                                                          gagaTATTGCAATTCACCAGACGCCGAGTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                               <  2:3413135/140‑1 (MQ=255)
                                                                                          gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                               <  2:1013133/140‑1 (MQ=255)
                                                                                          gagaTATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAggc                                               <  1:182569/140‑2 (MQ=255)
                                                                                            gagatTGCAACTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                             <  1:2719380/137‑1 (MQ=255)
                                                                                            gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                             <  2:3983107/140‑1 (MQ=255)
                                                                                            gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                             <  1:2013188/140‑1 (MQ=255)
                                                                                            gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                             <  2:2027169/140‑1 (MQ=255)
                                                                                            gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                             <  1:4018405/140‑1 (MQ=255)
                                                                                            gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                             <  1:3876199/140‑1 (MQ=255)
                                                                                               atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTCTATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                          >  1:116474/1‑140 (MQ=255)
                                                                                               atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                          >  1:293482/1‑140 (MQ=255)
                                                                                                   cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTataat                                      >  2:2496133/1‑140 (MQ=255)
                                                                                                   cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATGTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTataat                                      >  2:3880523/1‑140 (MQ=255)
                                                                                                     aGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCCTTTGAAACCTGTGTCGATCGGAACGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTAtaataa                                    >  2:3580036/1‑140 (MQ=255)
                                                                                                        cACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGcc                                 >  2:1764370/1‑140 (MQ=255)
                                                                                                         aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                <  2:646010/140‑1 (MQ=255)
                                                                                                         aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                <  2:2930419/140‑1 (MQ=255)
                                                                                                         aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                <  2:3491994/140‑1 (MQ=255)
                                                                                                           cATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAAACTGTGTAGATCGGAAAGCATGGTTAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCggg                              >  2:1376754/1‑140 (MQ=255)
                                                                                                                    aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                     >  1:1020413/1‑140 (MQ=255)
                                                                                                                    aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                     >  2:3611793/1‑140 (MQ=255)
                                                                                                                    aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                     >  2:3222199/1‑140 (MQ=255)
                                                                                                                    aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCATGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGTCTACGATAATAAGCCGGGTCTTACGGc                     >  2:1412708/1‑140 (MQ=255)
                                                                                                                     ttGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCt                    >  1:3555294/1‑140 (MQ=255)
                                                                                                                        gacaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaa                 <  2:3654797/137‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGACAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCGTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTCTAATAAGCCGGGTCTTACGGCTGaaa                <  2:3717814/140‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                <  1:1607672/140‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                <  2:1157890/140‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                <  1:3242986/140‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                <  1:2481256/140‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                <  2:243477/140‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                <  2:2209097/140‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                <  1:1839169/140‑1 (MQ=255)
                                                                                                                         aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                <  2:1730276/140‑1 (MQ=255)
                                                                                                                          aaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaaa               >  1:3025166/1‑140 (MQ=255)
                                                                                                                                gTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtat         >  1:934405/1‑140 (MQ=255)
                                                                                                                                 tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata        <  1:2929084/140‑1 (MQ=255)
                                                                                                                                 tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata        <  1:3661245/140‑1 (MQ=255)
                                                                                                                                 tctcCAACATGAAAATTTATAGTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata        >  2:1664778/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CGGTATCGATCTTTAAAAACTGCCGTGTTGAAAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAAC  >  NZ_CP034943/1805427‑1805701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: