Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,805,596 A→G 6.0% K792K (AAA→AAG EO946_RS09140 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,805,5960AG 6.0% 706.5 / 23.7 216K792K (AAA→AAGEO946_RS09140non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (107/95);  new base G (6/7);  total (113/103)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 218 total aligned reads displayed.

AAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTTATA  >  NZ_CP034943/1805458‑1805729
                                                                                                                                          |                                                                                                                                     
aaaTATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAAc                                                                                                                                      <  1:1464727/140‑1 (MQ=255)
aaaTATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAAc                                                                                                                                      <  1:1635512/140‑1 (MQ=255)
        aCGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCg                                                                                                                              <  2:1152252/140‑1 (MQ=255)
              cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                                                        >  1:469398/1‑140 (MQ=255)
              cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                                                        <  2:1903887/140‑1 (MQ=255)
              cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGAAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGAGGACCAGCATTAGAAACATGTGTCGATCGGa                                                                                                                        >  1:2785867/1‑140 (MQ=255)
                acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                                                      >  1:3490459/4‑140 (MQ=255)
                acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                                                      >  1:646010/4‑140 (MQ=255)
                aCTGAAGCCTCCATTTACGCTGCTTATTTCGGATGCGGAAAAGGAGATAATGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                                                      <  2:2487941/140‑1 (MQ=255)
                aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaca                                                                                                                      <  2:1974024/140‑3 (MQ=255)
                aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                                                      >  2:3224222/1‑140 (MQ=255)
                      gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTgg                                                                                                                >  1:1334070/1‑140 (MQ=255)
                       ccTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCAACATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTTTCGATCGGAAAGCCTGGt                                                                                                               <  2:3618669/140‑1 (MQ=255)
                        cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                                              >  1:800151/1‑140 (MQ=255)
                        cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                                              >  1:3629566/1‑140 (MQ=255)
                        cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                                              >  2:1646900/1‑140 (MQ=255)
                         tCCATTTACGCTGCGTATTTCGGATGCGGAACAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGACAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGa                                                                                                             >  2:2186944/1‑140 (MQ=255)
                          ccATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAg                                                                                                            <  2:72615/140‑1 (MQ=255)
                           cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGc                                                                                                           >  1:2152520/1‑140 (MQ=255)
                           cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                                                           >  1:398452/1‑140 (MQ=255)
                           cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                                                           >  1:2535742/1‑140 (MQ=255)
                           cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                                                           >  1:2121594/1‑140 (MQ=255)
                               tACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTg                                                                                                       <  1:223658/140‑1 (MQ=255)
                                aCGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGc                                                                                                      >  2:3242986/1‑140 (MQ=255)
                                   cTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATc                                                                                                   >  1:3505912/1‑140 (MQ=255)
                                     gCGTATTTCGGATGCGGAAAAGGATATATTGCAAGTCACCATACGCCGATTGGAAAGACTGTCTCCAACATGAAAATTTATATTGTGGACAAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGAATctc                                                                                                 >  2:1056874/1‑138 (MQ=255)
                                     gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                                                                 >  1:2209097/1‑140 (MQ=255)
                                     gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                                                                 >  1:346070/1‑140 (MQ=255)
                                     gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                                                                 >  1:53834/1‑140 (MQ=255)
                                      cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGt                                                                                                               <  1:1191690/125‑1 (MQ=255)
                                      cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                                                                                >  2:2654774/1‑140 (MQ=255)
                                      cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                                                                                >  2:519038/1‑140 (MQ=255)
                                          tttCTGATGCGGAAAAGGAGATATTGCAAGTGACCCTCCGCCGCTTGGAAAGCATGTCTCCAAGATCAAAATTTATCTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGCTTTGAATCGCATGCg                                                                                            <  1:1700981/140‑1 (MQ=255)
                                          tttCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCg                                                                                            <  1:1665931/140‑1 (MQ=255)
                                               gATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGt                                                                                       <  2:3080841/140‑1 (MQ=255)
                                                aTGCGGAAAAGGAGATTTTGCAAGTCACCCTACGCCGATTGGAAAGCCTCTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGATACCTGTGTCGATCGGAAAGCCGGGTGAGCTTTGCATAGCAGGAGACGGtg                                                                                      >  2:3638137/1‑139 (MQ=255)
                                                aTGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGtt                                                                                      >  2:3929665/1‑140 (MQ=255)
                                                aTGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGtt                                                                                      >  1:3804024/1‑140 (MQ=255)
                                                      aaaaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCaa                                                                                <  1:1122461/140‑1 (MQ=255)
                                                        aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                              <  2:1957052/140‑1 (MQ=255)
                                                        aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                              <  2:3891529/140‑1 (MQ=255)
                                                        aaGGAGATATTGCAAGGCACCATACGCCGATTGGAAAGCCTGGCTCCAACATGAAAATTTATATGGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                              <  2:2764855/140‑1 (MQ=255)
                                                        aaGGAGATATTGCAACTCCCCACACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATGGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                              <  2:1376225/140‑1 (MQ=255)
                                                           gagagaTCGCAAGTCACCATACACAGATTGGAAAGCCTGTTTCCAACACGAAAATTGATATTGTGGACCAGCATTTGAAGCCGGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAggc                                                                           <  1:182576/140‑2 (MQ=255)
                                                           gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTTCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                           >  2:1764737/1‑140 (MQ=255)
                                                           gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                           <  2:379511/140‑1 (MQ=255)
                                                           gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                           >  2:2622602/1‑140 (MQ=255)
                                                           gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                           <  2:1013133/140‑1 (MQ=255)
                                                             gagatTGCAACTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                         <  1:2719380/137‑1 (MQ=255)
                                                             gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                         <  1:4018405/140‑1 (MQ=255)
                                                             gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                         <  2:3983107/140‑1 (MQ=255)
                                                             gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                         <  1:2654774/140‑1 (MQ=255)
                                                             gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                         <  1:1928916/140‑1 (MQ=255)
                                                             gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                         <  2:1332769/140‑1 (MQ=255)
                                                             gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                         <  1:3139953/140‑1 (MQ=255)
                                                                atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                                                      >  2:1413585/1‑140 (MQ=255)
                                                                atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                                                      >  2:3661245/1‑140 (MQ=255)
                                                                atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                                                      >  1:293482/1‑140 (MQ=255)
                                                                    cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATCCTataat                                                                  >  2:450576/1‑140 (MQ=255)
                                                                    cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATAGTGTGGACCAGCATTTGAAACCTGTGTCGATCGGCAAGCCGGGTGAGGTTTGCATCGCAGGCGACGGTTTGGCACGAGGAGACTctaat                                                                  >  2:128353/1‑140 (MQ=255)
                                                                      aGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCCTTTGAAACCTGTGTCGATCGGAACGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTAtaataa                                                                >  2:3580036/1‑140 (MQ=255)
                                                                        tCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGc                                                              <  1:691795/140‑1 (MQ=255)
                                                                          aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                                            <  2:2930419/140‑1 (MQ=255)
                                                                          aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                                            <  2:646010/140‑1 (MQ=255)
                                                                          aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                                            <  2:3490459/140‑1 (MQ=255)
                                                                                     aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                                                 >  2:3222199/1‑140 (MQ=255)
                                                                                     aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                                                 >  1:1020413/1‑140 (MQ=255)
                                                                                     aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                                                 >  2:3397442/1‑140 (MQ=255)
                                                                                     aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                                                 >  2:3611793/1‑140 (MQ=255)
                                                                                      ttGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCt                                                >  2:1408512/1‑140 (MQ=255)
                                                                                          aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                                            <  1:3242986/140‑1 (MQ=255)
                                                                                          aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                                            <  2:1730276/140‑1 (MQ=255)
                                                                                          aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                                            <  1:2481256/140‑1 (MQ=255)
                                                                                          aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                                            <  2:1157980/140‑1 (MQ=255)
                                                                                           aaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaaa                                           >  1:2905426/1‑140 (MQ=255)
                                                                                           aaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaaa                                           >  1:3025166/1‑140 (MQ=255)
                                                                                                 gTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtat                                     >  1:878593/1‑140 (MQ=255)
                                                                                                 gTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtat                                     >  1:934405/1‑140 (MQ=255)
                                                                                                  tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata                                    <  1:3770039/140‑1 (MQ=255)
                                                                                                  tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata                                    <  1:2325627/140‑1 (MQ=255)
                                                                                                  tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata                                    <  1:1413585/140‑1 (MQ=255)
                                                                                                  tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata                                    <  1:2929084/140‑1 (MQ=255)
                                                                                                  tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata                                    <  1:3661245/140‑1 (MQ=255)
                                                                                                  tctcCAACATGAAAATTTATAGTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata                                    >  2:1664778/1‑140 (MQ=255)
                                                                                                        aCATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAAc                              >  2:1819616/1‑140 (MQ=255)
                                                                                                                   tctatTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                   <  2:2430459/138‑1 (MQ=255)
                                                                                                                   tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                   <  2:2854470/140‑1 (MQ=255)
                                                                                                                   tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                   <  1:1791608/140‑1 (MQ=255)
                                                                                                                   tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                   <  1:3040993/140‑1 (MQ=255)
                                                                                                                   tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                   <  1:3929665/140‑1 (MQ=255)
                                                                                                                            gACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACaaa          >  1:3899416/1‑140 (MQ=255)
                                                                                                                             aCCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAg         <  2:249629/140‑1 (MQ=255)
                                                                                                                             aCCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAg         <  2:800151/140‑1 (MQ=255)
                                                                                                                                aGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCtt      <  2:2246017/140‑1 (MQ=255)
                                                                                                                                 gCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGAt                                                                          >  2:607002/1‑71 (MQ=255)
                                                                                                                                  cATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                           <  2:3383620/69‑1 (MQ=255)
                                                                                                                                  cATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                           >  1:3383620/1‑69 (MQ=255)
                                                                                                                                  cATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTta    >  1:2034651/1‑140 (MQ=255)
                                                                                                                                  cATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTta    <  2:1538900/140‑1 (MQ=255)
                                                                                                                                    tttGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTtata  >  2:8496/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                     
AAATATTTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTTATA  >  NZ_CP034943/1805458‑1805729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: