Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,242 T→G 19.9% T274P (ACG→CCG)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2420TG19.9% 192.5 / 11.2 132T274P (ACG→CCG) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base T (58/42);  new base G (30/2);  total (88/44)
Fisher's exact test for biased strand distribution p-value = 8.39e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

ATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGT  >  NZ_CP034943/2502108‑2502379
                                                                                                                                      |                                                                                                                                          
atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGtgtgtg                                                                                                                                       >  1:1155583/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTgg                                                                                                                                      >  1:2859972/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTgg                                                                                                                                      >  2:277364/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGGGTgg                                                                                                                                      >  1:2226890/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATCCCCGGGTGTgg                                                                                                                                      >  1:2746212/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTGTCTTTCCCGATTTTAATCGAAAACCCCGTGTGTgg                                                                                                                                      >  2:147052/1‑140 (MQ=255)
      gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGAtt                                                                                                                                 >  1:209703/1‑140 (MQ=255)
           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                            <  1:109199/140‑1 (MQ=255)
           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                            <  1:3595615/140‑1 (MQ=255)
           tCGGACCGATGATCGAAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                            <  1:270503/140‑1 (MQ=255)
               aCCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCt                                                                                                                       <  2:542259/140‑1 (MQ=255)
                   atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGCTTGAACGAGCTTTaa                                                                                                                   >  2:1528164/1‑140 (MQ=255)
                   atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGTTGGGGCTTGAACGTGCTTTaa                                                                                                                   >  2:132003/1‑140 (MQ=255)
                   atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTaa                                                                                                                   >  2:896489/1‑140 (MQ=255)
                        cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCAAGAATTTAATAGAAAAACACGGGTGTGTGTATTTAAAGACAGTTaaaaaaa                                                                                                              >  1:1123609/1‑137 (MQ=255)
                        cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATATTAATCGAAATACCCGGGTGTGGGGATTTAACGAGCGTTAAAAAAt                                                                                                              >  1:1991602/1‑140 (MQ=255)
                        cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGAATTTAATAGAAATCCCCGTGTGTGGGGATTTAACCAGCATTAAAAAAt                                                                                                              >  1:708467/1‑140 (MQ=255)
                        cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGCTTTTAATAGAAATACCCGGGTGGGGGGATTGAAAGAGCGTTaaaaaaa                                                                                                              >  1:1398501/1‑137 (MQ=255)
                             ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAGGAGCGTTAAAACATCCTGt                                                                                                         >  2:2367401/1‑140 (MQ=255)
                             ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAAGAGCTTTAAACACTCCTGt                                                                                                         >  2:3554356/1‑140 (MQ=255)
                             ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACAACCTGt                                                                                                         >  2:1972963/1‑140 (MQ=255)
                             ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATAGAAAAACCCGTGGGTGGGGCTTGAACGAGCGTTAAAACAACCAGt                                                                                                         >  2:404363/1‑140 (MQ=255)
                                aGCCGATCTGCTTCTGATCAACGGTATAAGTAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGGTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTTGACGAGCTTTAAAACATCCTGttgt                                                                                                      >  2:1798954/1‑138 (MQ=255)
                                  ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                    <  2:1155583/140‑1 (MQ=255)
                                  ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                    <  1:3368571/140‑1 (MQ=255)
                                    gATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttt                                                                                                  <  2:1109981/140‑1 (MQ=255)
                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGCTTTTAATCGACAGACCCCTGTGGGGGGCTTTAGGGAGCGTTAAAAGACCCTGTTAtttttt                                                                                                 >  1:576487/1‑139 (MQ=255)
                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTTATAGAAATACCCGTGTGTGGGGGTTTAACGAGCTTTTAAACATCCGGTTGttttta                                                                                                 >  1:2288730/1‑139 (MQ=255)
                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTTTTCttttta                                                                                                 >  2:2906402/1‑139 (MQ=255)
                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGACCGAGCGTTAAAACATCCTGTTCttttta                                                                                                 >  2:1905626/1‑139 (MQ=255)
                                       cTGCTTCTGATCAACTGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaa                                                                                               <  1:338809/140‑1 (MQ=255)
                                       cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaa                                                                                               <  2:922913/140‑1 (MQ=255)
                                       cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAAAATCAGGTTCTTTTTCaa                                                                                               >  2:161967/1‑140 (MQ=255)
                                          cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAAAATACTGTTCTTTTTAAATTa                                                                                            >  1:460688/1‑140 (MQ=255)
                                          cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTa                                                                                            >  2:3488388/1‑140 (MQ=255)
                                          cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAAACGAAAACCCCGTGGGGGGGGATTGGAAGTGGGGTTAAACAGCCTGTTATTTTTGAATTa                                                                                            >  1:2052963/1‑140 (MQ=255)
                                              tGATAAACGCAATAAGAAGCAGTAATCAGACTGCATTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCTTTTTTAATCGAATTCCCCGTGTGTGGGTTTTGAACCAGCTTTAAAACATCCTGTTCTTTTTCAATTAATga                                                                                        <  2:2994268/140‑1 (MQ=255)
                                                aTCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAAGATCCTGTTCTTTTTCAATTAATgaga                                                                                      >  2:650206/1‑140 (MQ=255)
                                                aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATgtga                                                                                      >  2:1671229/1‑140 (MQ=255)
                                                aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGGTTTCTTTCCCGATTTTAATCGAAATCCGCGGGGGTGGGGATTGAACGAGCTTTAAAACATGGTGTTCTTTTTCAATTAATgtta                                                                                      >  2:2868172/1‑137 (MQ=255)
                                                    agggAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCTATTTTAATCTAAATCCCCGTGTGTGGGGTTTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                                                                  <  1:695015/138‑1 (MQ=255)
                                                     cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCCATTTTAATACAAAAACCCCTGGGTGGGGATTGAAAGAGCTTTAAAACATCCGGTTCTTTTTTAATTAATGATAGAAGc                                                                                 >  1:535014/1‑140 (MQ=255)
                                                     cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATACAAATCCCCGTGGGTGGGGATTGAACCAGCTTTAAAAAATCCTGTTATTTTTCAAATAATGATAGaacc                                                                                 >  1:3082264/1‑138 (MQ=255)
                                                     cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGAATTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGc                                                                                 >  2:1765120/1‑140 (MQ=255)
                                                       gAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGa                                                                               <  2:1433463/140‑1 (MQ=255)
                                                       gAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGa                                                                               <  2:678224/140‑1 (MQ=255)
                                                         aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                             <  1:1388670/140‑1 (MQ=255)
                                                         aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                             <  2:2226890/140‑1 (MQ=255)
                                                         aTAAGAAGCAGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAAGCGAcc                                                                             <  1:1651842/140‑1 (MQ=255)
                                                          tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                            <  2:2110623/140‑1 (MQ=255)
                                                          tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                            <  2:139783/140‑1 (MQ=255)
                                                          tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                            <  2:3874359/140‑1 (MQ=255)
                                                            cgaagCCGTAATCAGACTGCAGTTTTCCCTCTCTTCATAGTCGTTTACTTGCCCGATTTTAATCGAAAGCCCCGGGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATCAGAGAAGCGACCGaa                                                                          <  2:1565798/139‑1 (MQ=255)
                                                              aagCCGTAATCAGACTGCAGTATTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAct                                                                        <  1:3222071/140‑1 (MQ=255)
                                                                gCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATTTTAATCGAAATACCCGTGTGTGGGGGTTTAAAGAGCTTTAAAACATCCTGTTATTTTTGAATTAATTAGAGAAGCCAACGAActat                                                                      >  1:1499426/1‑138 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATGCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                    >  2:2128881/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATTAGAGAAGCGACCGAACTCTgg                                                                    >  2:165771/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                    >  2:3097500/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                    >  2:920338/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATTAGAGAAGCGACCGAACTCggg                                                                    >  2:188382/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                    >  2:1999756/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGCTTTTTATCGAAATACCCCTGTGTGGGGATTTTACGAGGTTTAAAACAGCCTGTTGTTTTTCAATTAATTATCGAAGCGACCGAACTCTgg                                                                    >  1:1788319/1‑140 (MQ=255)
                                                                  cGTAAACAGACTGCAGTTTTCCCTCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                    <  2:827268/140‑1 (MQ=255)
                                                                     aaTCAGACTGCATTTTTCAATCTCTTAATAGTCGTTTTCTTTCCCGATTTTAATCGACATCCCCGTTTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                                                                 <  1:3755244/140‑1 (MQ=255)
                                                                     aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTACTGAGAGAAGCGACCGAACTCTGGTGa                                                                 <  2:2151356/140‑1 (MQ=255)
                                                                     aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCCAAATCCCCCGGTGTGGGGATTGAACCAGCTTTAAAACATGCTGTTCTTTTTAAATTAATGACAGAAGCGACCGAACTCTGGTGa                                                                 >  2:2865682/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATTTTAATAGAAAACCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATACGGTTATTTTTGAATTAATGAGATTAGCGAACGAACACTGGTGATATAAt                                                           >  1:683492/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGAGTTTAATCGAAATACCCGGGTGTGGGGATTGAACGAGCGTTAAAAAATCCTGTTGTTTTTAAATTAATTAGAGAAGCGACCGCACTATGGTGATATCAt                                                           >  1:3155227/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGCTTTTAATCGAAATCCCCGTGTGTGGGGATTTGACGAGCGTTAAAACAGGCTGTTATTTTTGACTTTATGATGGACGCGACCGACCTCTGGGGATATCAt                                                           >  1:1026963/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTAGTTCCCCATTTTAATGGAAAAAACCGTGTGTGGGGTTTTAACGAGGGTTAAAAAATGCAGTTATTTTTAAATTAAAGAGAGAATCGACCCGACTAAGGTGATATCAt                                                           >  1:798490/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTGGTTCCCGCTTTTAATCGAAAAACCCGGGTGTGGGGATTGAACGAGCGTTAAAACATCCGGTTGTTTTTGAATTAATGAGAGAAGCGACCGACCTCTGGGGATATCAt                                                           >  1:759631/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATGGAAAACCCCGGGGTTGGGGATTGAAAGAGCGTTAAAAAATCCTGTTCTTTTTTAAATAATTAGTGAAACGACCGAACTCGGGTGGTATCAt                                                           >  1:1824019/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCCATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGGGTTAAAACAAGCTGTTATTTTTCAATTAATGAGAGAAGCGCCCGAACTCGGGTGCTATAAt                                                           >  1:266991/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTAGTTCCCCATTTTAAACGAAATCCCCGTGTGGGGGGATTGAAACCGATTTAAAAAAACCGGTTATATTTAAAATAATGAGAGAAGAGGCCGAAAACGGGTGATATCAt                                                           >  1:2977446/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCGTCATAGTCGTTGTCGTTCCCGATTTTAATCGTCATCCCCGGGGGTTGGGATTGTACGTGCGTTTAAAACTGCGGTTGTTTTTGTTTTAATGAGGGTCGCGACCGACCTGGGGTggtgtgat                                                           >  1:3541517/1‑133 (MQ=255)
                                                                             tGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGCTTGAACGGGCTTTAAAAAATGGTTTTCTTTTTCAAATAATTAGGGAAGCGACCGAACAATGGTGATATCATgg                                                         >  1:3968143/1‑140 (MQ=255)
                                                                             tGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTAATCGAAATACCCGGGGGTGGGGATTGAACGAGCTTTAAAACATGCTGTTCTTTTTGAATTAATTAGAGAAGCGACCGCACTCTGGTGTTATCATgg                                                         >  1:551788/1‑140 (MQ=255)
                                                                             tGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAATCCCCGGGGGTGGGGATTGTACGAGCTTTTAAAAATGCTGTTGTTTTTAAATTAATGTGAGAATCCCCCGAACGCGGGGGATATAATgg                                                         >  1:816533/1‑140 (MQ=255)
                                                                             tGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTGCCGAATTTAATAGAAATCCCCGGGTGGGGGGATTTTACGTGCTTTAAAAAATGCTGTTCTTTTTGAATTAAAGAGAGAAGCGAACGAAAACTGGTGATATCAttg                                                         >  1:2312562/1‑138 (MQ=255)
                                                                                 gTTTTCCATCTCTTCATAGTCGTTTTCTGTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGCGAGAAGCGACCGAACTCTGGTGATATCATGGAACt                                                     <  1:3074447/140‑1 (MQ=255)
                                                                                 gTTTTCCATCTATTCATATTCGTTTTCGTTGACGATTTTCATGGAATTCCCAGTGGGTGGGGATTGAACGAGCTTTACAACATCCGGTTCTTTTACACTGAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACt                                                     <  2:1214998/140‑1 (MQ=255)
                                                                                    ttCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAAAGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGATAAGCGACCGAACTCTGGGGTTATAAAGGAACTACg                                                  >  2:1637087/1‑140 (MQ=255)
                                                                                     tCCATCTCTTCATCGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTGTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCgg                                                 <  2:3070810/140‑1 (MQ=255)
                                                                                     tCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTGAAATAATGAGGGAATCGACCGAACACTGGTGATATCATTGAACTCCgg                                                 >  1:3198081/1‑140 (MQ=255)
                                                                                     tCCATCTCTTCATAGTCGTTTTCTTTCCCGATTGTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCgg                                                 <  2:2444164/140‑1 (MQ=255)
                                                                                     tCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTTATCGAAATAACCGGGTGTGGGGGTTGAACGCGCTTTAAAAAATACGGTTCTTTTTGAATTAAATAGAGAACCGACCGCAATCTGGTGGTATGAATGAACTACgg                                                 >  1:3648305/1‑140 (MQ=255)
                                                                                      ccATCTCTTCATCGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                <  2:1795355/140‑1 (MQ=255)
                                                                                      ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGACCTCTGGTGATATCATGGAACTCCGGc                                                <  2:1795378/140‑1 (MQ=255)
                                                                                      ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                <  2:1991602/140‑1 (MQ=255)
                                                                                      ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                <  2:1398501/140‑1 (MQ=255)
                                                                                               cATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCGGGGGATATCATGGAACTCCGGCTGGGTCAGc                                       >  2:1892179/1‑140 (MQ=255)
                                                                                                         ttggttcCCGATTTTAATCGAAATACCCGGGTGTGGGGATTTAACGAGCGTTAAAACATTCTGTTCTTTTTCAATTAATTAGAGAAACCAACCAACACCTGTTCTATCATTGAACAACGGCAGGTTAAGCAAAATGAttt                             >  2:2549948/5‑140 (MQ=255)
                                                                                                         ttctttcCCGCTTTTAATAGAAATCCCCCTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAAGCGACCGAACTCTGGTGAGATAAAGGAACTCCGGCTGGTTCAGCATatttattt                             >  2:2845863/1‑140 (MQ=255)
                                                                                                         ttatttcCCGATTTTAATCGAAAAACCCGTGGGTGGGGATTGAACCAGCTTTAAAAAATACTGTTATTTTTAAATTAATTTGATAACCGACAGAACTCTTGTGATATAAATGAACACCCGCTGGGTCACCATatttattt                             >  2:3465986/4‑140 (MQ=255)
                                                                                                                   aTTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGaa                   >  1:298077/1‑140 (MQ=255)
                                                                                                                   aTTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGaa                   >  1:2806745/1‑140 (MQ=255)
                                                                                                                                    cGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt  >  1:195650/1‑140 (MQ=255)
                                                                                                                                    cGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt  >  1:195085/1‑140 (MQ=255)
                                                                                                                                    cGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt  >  1:3978562/1‑140 (MQ=255)
                                                                                                                                    cGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt  >  1:195619/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                          
ATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGT  >  NZ_CP034943/2502108‑2502379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: