Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,244 T→G 11.5% H273P (CAC→CCC)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2440TG11.5% 244.8 / 3.0 132H273P (CAC→CCC) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base T (69/41);  new base G (19/3);  total (88/44)
Fisher's exact test for biased strand distribution p-value = 4.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGAT  >  NZ_CP034943/2502108‑2502382
                                                                                                                                        |                                                                                                                                           
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATTTTAATAGAAATCCCCGGGGGTgg                                                                                                                                         >  1:1957445/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTgg                                                                                                                                         >  1:2859972/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGGGTgg                                                                                                                                         >  1:2226890/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATCCCCGGGTGTgg                                                                                                                                         >  1:2746212/1‑140 (MQ=255)
 ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTGTCTTTCCCGATTTTAATCGAAAACCCCGTGTGTgg                                                                                                                                         >  2:147052/1‑140 (MQ=255)
      gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGAtt                                                                                                                                    >  1:209703/1‑140 (MQ=255)
      gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATCGAAATCCCCGTGTGTGGGGAtt                                                                                                                                    >  1:1844181/1‑140 (MQ=255)
      gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTATTTCCCGATTTTAATAGAAATCACCGGGTGTGGGGAtt                                                                                                                                    >  1:1236529/1‑140 (MQ=255)
           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                               <  1:109199/140‑1 (MQ=255)
           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                               <  1:2387057/140‑1 (MQ=255)
           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                               <  2:2219306/140‑1 (MQ=255)
           tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCAGAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                               <  1:3543027/140‑1 (MQ=255)
           tCGGACCGATGATCGAAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                               <  1:270503/140‑1 (MQ=255)
               aCCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCt                                                                                                                          <  2:542259/140‑1 (MQ=255)
                   atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCCGTTGTCGATCTCTTCATAGTCGTTGTCTTTCAAGATTTTAATCGAAATCCCCGTTGGTGGGGGTTTAACGAGCTTTaa                                                                                                                      >  2:2082448/1‑140 (MQ=255)
                   atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGCTTGAACGAGCTTTaa                                                                                                                      >  2:1528164/1‑140 (MQ=255)
                   atgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGTTGGGGCTTGAACGTGCTTTaa                                                                                                                      >  2:132003/1‑140 (MQ=255)
                       tCGCAAGTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCCTAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACa                                                                                                                  <  2:19239/140‑1 (MQ=255)
                        cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCAAGAATTTAATAGAAAAACACGGGTGTGTGTATTTAAAGACAGTTaaaaaaa                                                                                                                 >  1:1123609/1‑137 (MQ=255)
                        cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATATTAATCGAAATACCCGGGTGTGGGGATTTAACGAGCGTTAAAAAAt                                                                                                                 >  1:1991602/1‑140 (MQ=255)
                        cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGAATTTAATAGAAATCCCCGTGTGTGGGGATTTAACCAGCATTAAAAAAt                                                                                                                 >  1:708467/1‑140 (MQ=255)
                        cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGCTTTTAATAGAAATACCCGGGTGGGGGGATTGAAAGAGCGTTaaaaaaa                                                                                                                 >  1:1398501/1‑137 (MQ=255)
                             ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAGGAGCGTTAAAACATCCTGt                                                                                                            >  2:2367401/1‑140 (MQ=255)
                             ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAAGAGCTTTAAACACTCCTGt                                                                                                            >  2:3554356/1‑140 (MQ=255)
                             ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACAACCTGt                                                                                                            >  2:1972963/1‑140 (MQ=255)
                             ttGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATAGAAAAACCCGTGGGTGGGGCTTGAACGAGCGTTAAAACAACCAGt                                                                                                            >  2:404363/1‑140 (MQ=255)
                                  ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                       <  2:1155583/140‑1 (MQ=255)
                                  ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCGGTTCttt                                                                                                       >  2:856352/1‑140 (MQ=255)
                                    gATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttt                                                                                                     <  2:1109981/140‑1 (MQ=255)
                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGCTTTTAATCGACAGACCCCTGTGGGGGGCTTTAGGGAGCGTTAAAAGACCCTGTTAtttttt                                                                                                    >  1:576487/1‑139 (MQ=255)
                                     aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTTTTCttttta                                                                                                    >  2:2906402/1‑139 (MQ=255)
                                       cTGCTTCTGATCAACTGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaa                                                                                                  <  1:338809/140‑1 (MQ=255)
                                       cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaa                                                                                                  <  2:922913/140‑1 (MQ=255)
                                       cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTGCCGATTTTAATAGAAAACCCCGTGTGTGGGGATTGAACGAGGTTTAAAACATCCTGGTCTTTTTCaa                                                                                                  >  1:2207261/1‑140 (MQ=255)
                                       cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAAAATCAGGTTCTTTTTCaa                                                                                                  >  2:161967/1‑140 (MQ=255)
                                       cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCAACTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaa                                                                                                  <  1:3954796/140‑1 (MQ=255)
                                          cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAAAATACTGTTCTTTTTAAATTa                                                                                               >  1:460688/1‑140 (MQ=255)
                                          cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGTACGCGCTTTAAAACATCGGGTTGTTTTTGAAttt                                                                                               >  1:3097218/1‑139 (MQ=255)
                                          cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCCATTTTAATAGAAATACCCGGGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTa                                                                                               >  1:2796718/1‑140 (MQ=255)
                                              tGATAAACGCAATAAGAAGCAGTAATCAGACTGCATTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCTTTTTTAATCGAATTCCCCGTGTGTGGGTTTTGAACCAGCTTTAAAACATCCTGTTCTTTTTCAATTAATga                                                                                           <  2:2994268/140‑1 (MQ=255)
                                                aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATgaga                                                                                         >  2:644740/1‑140 (MQ=255)
                                                aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATgtga                                                                                         >  2:1671229/1‑140 (MQ=255)
                                                    agggAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCTATTTTAATCTAAATCCCCGTGTGTGGGGTTTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                                                                     <  1:695015/138‑1 (MQ=255)
                                                     cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCCATTTTAATACAAAAACCCCTGGGTGGGGATTGAAAGAGCTTTAAAACATCCGGTTCTTTTTTAATTAATGATAGAAGc                                                                                    >  1:535014/1‑140 (MQ=255)
                                                     cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATACAAATCCCCGTGGGTGGGGATTGAACCAGCTTTAAAAAATCCTGTTATTTTTCAAATAATGATAGaacc                                                                                    >  1:3082264/1‑138 (MQ=255)
                                                       gAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGa                                                                                  <  2:678224/140‑1 (MQ=255)
                                                       gAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGa                                                                                  <  2:1788319/140‑1 (MQ=255)
                                                       gAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGa                                                                                  <  2:1433463/140‑1 (MQ=255)
                                                         aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                                <  1:1388670/140‑1 (MQ=255)
                                                         aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                                <  2:2859972/140‑1 (MQ=255)
                                                         aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                                <  2:2226890/140‑1 (MQ=255)
                                                         aTAAGAAGCAGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAAGCGAcc                                                                                <  1:1651842/140‑1 (MQ=255)
                                                          tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                               <  2:3874359/140‑1 (MQ=255)
                                                          tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                               <  2:2110623/140‑1 (MQ=255)
                                                          tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                               <  2:139783/140‑1 (MQ=255)
                                                            cgaagCCGTAATCAGACTGCAGTTTTCCCTCTCTTCATAGTCGTTTACTTGCCCGATTTTAATCGAAAGCCCCGGGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATCAGAGAAGCGACCGaa                                                                             <  2:1565798/139‑1 (MQ=255)
                                                              aagCCGTAATCAGACTGCAGTATTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAct                                                                           <  1:3222071/140‑1 (MQ=255)
                                                                gCCGTAATCAGCCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAAGATCCGGTTCTTTTTCAATTAATTAGCGAAGCGACCGAActct                                                                         >  2:2454316/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATGCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                       >  2:2128881/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATTAGAGAAGCGACCGAACTCTgg                                                                       >  2:165771/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                       <  2:2924988/140‑1 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                       >  2:3097500/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                       >  2:920338/1‑140 (MQ=255)
                                                                  cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATTAGAGAAGCGACCGAACTCggg                                                                       >  2:188382/1‑140 (MQ=255)
                                                                  cGTAAACAGACTGCAGTTTTCCCTCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                       <  2:827268/140‑1 (MQ=255)
                                                                     aaTCAGACTGCATTTTTCAATCTCTTAATAGTCGTTTTCTTTCCCGATTTTAATCGACATCCCCGTTTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                                                                    <  1:3755244/140‑1 (MQ=255)
                                                                     aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCCAAATCCCCCGGTGTGGGGATTGAACCAGCTTTAAAACATGCTGTTCTTTTTAAATTAATGACAGAAGCGACCGAACTCTGGTGa                                                                    >  2:2865682/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATTTTAATAGAAAACCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATACGGTTATTTTTGAATTAATGAGATTAGCGAACGAACACTGGTGATATAAt                                                              >  1:683492/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCCATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCGTTAAAAAATCCTGTTGTTTTTGAATTAAATAGAGAAGCGACCCAACACTGGTGATATCAt                                                              >  1:3238984/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGCTTTTAATCGAAATCCCCGTGTGTGGGGATTTGACGAGCGTTAAAACAGGCTGTTATTTTTGACTTTATGATGGACGCGACCGACCTCTGGGGATATCAt                                                              >  1:1026963/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAGTGAACGAGCTTTAAAACAACCTGTTCTTTTTCAATTAAGTAGAGTAGCGACCGAACTCTGGTGATATCAt                                                              >  1:2977527/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTAGTTCCCCATTTTAATGGAAAAAACCGTGTGTGGGGTTTTAACGAGGGTTAAAAAATGCAGTTATTTTTAAATTAAAGAGAGAATCGACCCGACTAAGGTGATATCAt                                                              >  1:798490/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGCTTTTAATCGAAAAACCCCGGTGTGGGGGTTTTACGAGCTTTAAAAAATACTGTTGTTTTTAAAATAATGAGAGAAGCGACCGAAAACTGGGGATATAAt                                                              >  1:1566349/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTGGTTCCCGCTTTTAATCGAAAAACCCGGGTGTGGGGATTGAACGAGCGTTAAAACATCCGGTTGTTTTTGAATTAATGAGAGAAGCGACCGACCTCTGGGGATATCAt                                                              >  1:759631/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATGGAAAACCCCGGGGTTGGGGATTGAAAGAGCGTTAAAAAATCCTGTTCTTTTTTAAATAATTAGTGAAACGACCGAACTCGGGTGGTATCAt                                                              >  1:1824019/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTAGTTCCCCATTTTAAACGAAATCCCCGTGTGGGGGGATTGAAACCGATTTAAAAAAACCGGTTATATTTAAAATAATGAGAGAAGAGGCCGAAAACGGGTGATATCAt                                                              >  1:2977446/1‑140 (MQ=255)
                                                                           aCTGCAGTTTTCCATCTCGTCATAGTCGTTGTCGTTCCCGATTTTAATCGTCATCCCCGGGGGTTGGGATTGTACGTGCGTTTAAAACTGCGGTTGTTTTTGTTTTAATGAGGGTCGCGACCGACCTGGGGTggtgtgat                                                              >  1:3541517/1‑133 (MQ=255)
                                                                             tGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTAATCGAAATACCCGGGGGTGGGGATTGAACGAGCTTTAAAACATGCTGTTCTTTTTGAATTAATTAGAGAAGCGACCGCACTCTGGTGTTATCATgg                                                            >  1:551788/1‑140 (MQ=255)
                                                                             tGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAATCCCCGGGGGTGGGGATTGTACGAGCTTTTAAAAATGCTGTTGTTTTTAAATTAATGTGAGAATCCCCCGAACGCGGGGGATATAATgg                                                            >  1:816533/1‑140 (MQ=255)
                                                                             tGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTGCCGAATTTAATAGAAATCCCCGGGTGGGGGGATTTTACGTGCTTTAAAAAATGCTGTTCTTTTTGAATTAAAGAGAGAAGCGAACGAAAACTGGTGATATCAttg                                                            >  1:2312562/1‑138 (MQ=255)
                                                                                 gTTTTCCATCTCTTCATAGTCGTTTTCTGTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGCGAGAAGCGACCGAACTCTGGTGATATCATGGAACt                                                        <  1:3074447/140‑1 (MQ=255)
                                                                                 gTTTTCCATCTATTCATATTCGTTTTCGTTGACGATTTTCATGGAATTCCCAGTGGGTGGGGATTGAACGAGCTTTACAACATCCGGTTCTTTTACACTGAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACt                                                        <  2:1214998/140‑1 (MQ=255)
                                                                                    ttCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGCTTTAACGAGCTTTAAAACATCCTGTTCTTTTTGAATTAATTAGAGAAGCGACCGAAATCTGGTGATATAAAGTAACTCCg                                                     >  2:3605028/1‑140 (MQ=255)
                                                                                    ttCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCGTGGGGGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCAAATAATTAGAGAAACGCCCGAACTCTGGTGATATCATGGAACACCg                                                     >  2:3411490/1‑140 (MQ=255)
                                                                                     tCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTGAAATAATGAGGGAATCGACCGAACACTGGTGATATCATTGAACTCCgg                                                    >  1:3198081/1‑140 (MQ=255)
                                                                                     tCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTTATCGAAATAACCGGGTGTGGGGGTTGAACGCGCTTTAAAAAATACGGTTCTTTTTGAATTAAATAGAGAACCGACCGCAATCTGGTGGTATGAATGAACTACgg                                                    >  1:3648305/1‑140 (MQ=255)
                                                                                      ccATCTCTTCATCGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                   <  2:1795355/140‑1 (MQ=255)
                                                                                      ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGACCTCTGGTGATATCATGGAACTCCGGc                                                   <  2:1795378/140‑1 (MQ=255)
                                                                                      ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                   <  2:1398501/140‑1 (MQ=255)
                                                                                      ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                   <  2:1991602/140‑1 (MQ=255)
                                                                                               cATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCGGGGGATATCATGGAACTCCGGCTGGGTCAGc                                          >  2:1892179/1‑140 (MQ=255)
                                                                                                       tgttgtttcCCGATTTTAATAGAAATACCCGGGTGTGGGGGTTGAACGAGCTTTAAAAAATCCTGTTGTTTTTCAATTAATGAGATAATCGACCGAACTCTGGTGATATAATGGAACGCCGGCTGGTTCAGCATATTTAt                                  >  2:1814139/6‑140 (MQ=255)
                                                                                                       tgttctttcCCGATTTTAATCGAAAACCCCGGGTGTGGGGCTTGAACGAGCTTTAAAACATCCTGTTGTTTTTCAATTAATGAGAGAAGCGACCGAACTCTTGTGATATCATGGAACTCCGGCTGGTTCAGCATATTTAt                                  >  2:1641579/3‑140 (MQ=255)
                                                                                                         ttggttcCCGATTTTAATCGAAATACCCGGGTGTGGGGATTTAACGAGCGTTAAAACATTCTGTTCTTTTTCAATTAATTAGAGAAACCAACCAACACCTGTTCTATCATTGAACAACGGCAGGTTAAGCAAAATGAttt                                >  2:2549948/5‑140 (MQ=255)
                                                                                                         ttctttcCCGCTTTTAATAGAAATCCCCCTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAAGCGACCGAACTCTGGTGAGATAAAGGAACTCCGGCTGGTTCAGCATatttattt                                >  2:2845863/1‑140 (MQ=255)
                                                                                                         ttatttcCCGATTTTAATCGAAAAACCCGTGGGTGGGGATTGAACCAGCTTTAAAAAATACTGTTATTTTTAAATTAATTTGATAACCGACAGAACTCTTGTGATATAAATGAACACCCGCTGGGTCACCATatttattt                                >  2:3465986/4‑140 (MQ=255)
                                                                                                                                    cgggtgtgGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt     >  1:1658932/4‑140 (MQ=255)
                                                                                                                                    cGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt     >  1:195085/1‑140 (MQ=255)
                                                                                                                                    cGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt     >  1:195650/1‑140 (MQ=255)
                                                                                                                                    cGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt     >  1:3978562/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
ATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGAT  >  NZ_CP034943/2502108‑2502382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: