BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,864,573	T→G	5.2%	T415T (ACA→ACC)	pulA ←	type I pullulanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,864,573	0	T	G	5.2%	370.0 / 3.7	129	T415T (ACA→ACC)	pulA	type I pullulanase
Reads supporting (aligned to +/- strand): ref base T (42/76); new base G (5/4); total (49/80)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CGATGCCCGGCATTTTTGTAGCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACGCCGGTGC  >  NZ_CP034943/2864437‑2864712

cGATGCCCGGCATTTTTGTAGCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTAACGCAGTTTCGTTTTGTTTGATATAAAACACTGTa                                                                                                                                          >  1:284722/1‑140 (MQ=255)

     cccGGCATTTTTGTAGCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTTTCGCCAAATCCCACCCTTCTCAAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCATTCATATAAAGCACTGTATCAat                                                                                                                                     <  2:1211620/140‑1 (MQ=255)

     cccGGCATTTTTGTAGCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAat                                                                                                                                     <  1:2228037/140‑1 (MQ=255)

     cccGGCATTTTTGTAGCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAat                                                                                                                                     <  2:3247867/140‑1 (MQ=255)

     cccGGCATTTTTGTAGCGGTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAat                                                                                                                                     <  2:2466398/140‑1 (MQ=255)

               ttGTAGCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATg                                                                                                                           <  2:1800129/140‑1 (MQ=255)

                 gTAGCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGcc                                                                                                                         <  1:2938296/140‑1 (MQ=255)

                    gCGTTCGCCAAGGTCGCTTTTTTTTGTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTATTTAATATAAATCACGGTATCAATATATAAAAATCCAAg                                                                                                                      >  1:1382082/1‑140 (MQ=255)

                    gCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAACATGCCAAg                                                                                                                      <  2:1257819/140‑1 (MQ=255)

                    gCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAg                                                                                                                      <  1:4023304/140‑1 (MQ=255)

                    gCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAg                                                                                                                      <  2:1456326/140‑1 (MQ=255)

                    gCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAg                                                                                                                      <  2:1270119/140‑1 (MQ=255)

                    gCGTTCGCCAAGGTCGCTTTTTGTACGTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAg                                                                                                                      <  1:2955895/140‑1 (MQ=255)

                    gCGGTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAg                                                                                                                      <  2:836771/140‑1 (MQ=255)

                       ttCGCCAAGGTCGCTTTTTTTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCATGATTCCAGGCTTTACCGCAGTTTCTTTTTAGTTCCTAAAAAACCCAGTGTAAATATATAAAATGCCCAGccg                                                                                                                   >  1:583667/1‑138 (MQ=255)

                       ttCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCGCCAAAAAGCAGGAGTCCAGGCTTTACCGCAGTTGCTTTTTCTTTAATATAAAACACTGTGTCAATATATAAAATTCCAAGCaa                                                                                                                   >  1:707554/1‑139 (MQ=255)

                         cGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGAt                                                                                                                 <  1:1441260/140‑1 (MQ=255)

                         cGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCGTTTTCTTTGATATAAAGCACGGGATGAATAGATTAAATGCCAAGCCGAt                                                                                                                 >  1:1031658/1‑140 (MQ=255)

                           ccAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCa                                                                                                               <  1:2783382/140‑1 (MQ=255)

                               ggTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATTCCAAGCAGATCAAAGc                                                                                                           >  2:3423083/1‑140 (MQ=255)

                               ggTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAAAATCTAAAATTCCAAGCAGATCAAAGc                                                                                                           >  2:2384971/1‑140 (MQ=255)

                               ggTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTATTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGc                                                                                                           >  2:2113459/1‑140 (MQ=255)

                                 tCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCAGATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCgg                                                                                                         <  2:893529/140‑1 (MQ=255)

                                   gCTTTTTTTTGTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCGCCAAAAAGCAGGATTCCAGGCGGTAACGCATTTGGTTTTTGTTTGATATAAATCACTTTATCAAAATCTAAAATGGCAACCACATCCAAGCGGaa                                                                                                       >  1:167383/1‑140 (MQ=255)

                                     tttttGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGc                                                                                                     <  1:3038182/140‑1 (MQ=255)

                                        ttGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGt                                                                                                  <  1:3576984/140‑1 (MQ=255)

                                        ttGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGt                                                                                                  <  1:3242009/140‑1 (MQ=255)

                                        ttGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGt                                                                                                  <  2:2686082/140‑1 (MQ=255)

                                             catGCGGCAGCGGTGTAGCCAGGTCCCACCCTTCTCCAAAAAGCAGGATTCCGGGCTTTGCCTTAGTTGCTTTCTCTTTCATATAAAGCACGGTGTCAATATCTAAAATCCCGAGGAGATCAAAGCGGAAGCCGTcaaca                                                                                             <  1:2155736/138‑5 (MQ=255)

                                             catGCGGCAGCGGTGTAGCCAGGTCCCACCCTTCTCCAAAAAGCAGGATTCCGGGCTTTGCCTTAGTTGCTTTCTCTTTCATATAAAGCACGGTGTCAATATCTAAAATCCCGAGGAGATCAAAGCGGAAGCCGTcaaca                                                                                             <  1:818274/138‑5 (MQ=255)

                                             cTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATACAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCa                                                                                             <  1:3208167/140‑1 (MQ=255)

                                             cTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCa                                                                                             <  1:7579/140‑1 (MQ=255)

                                             cTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCa                                                                                             <  1:2045746/140‑1 (MQ=255)

                                             cTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCa                                                                                             <  2:3699090/140‑1 (MQ=255)

                                               tGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATc                                                                                           >  1:3857462/1‑140 (MQ=255)

                                                  ggCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTa                                                                                        <  1:3381093/140‑1 (MQ=255)

                                                  ggCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTa                                                                                        <  1:207149/140‑1 (MQ=255)

                                                       agGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCt                                                                                   <  1:2790353/140‑1 (MQ=255)

                                                       agGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCt                                                                                   <  2:3857462/140‑1 (MQ=255)

                                                       agGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCt                                                                                   <  1:3671993/140‑1 (MQ=255)

                                                            tCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCCATATCTAAAATGGCAAGCAGATCAAATCGGAAGCCGTCGGCCTCGTATTCCGCAATc                                                                              >  1:3277758/1‑140 (MQ=255)

                                                            tCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCa                                                                                 <  2:1562399/137‑1 (MQ=255)

                                                            tCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATc                                                                              <  1:2572164/140‑1 (MQ=255)

                                                            tCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTACTAAATc                                                                              >  1:3547161/1‑140 (MQ=255)

                                                            tCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAACCACTGTCTCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATc                                                                              >  1:946498/1‑140 (MQ=255)

                                                              gCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTGCTATAAACCAATGTATCAAAATCTAAAATTCCAAGCAGATCAAAGCGCAAGCCCTCGGCAACGTATTCCTCAATCCa                                                                            >  1:332925/1‑140 (MQ=255)

                                                              gCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCa                                                                            <  1:351546/140‑1 (MQ=255)

                                                              gCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCa                                                                            <  1:4005627/140‑1 (MQ=255)

                                                              gCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCa                                                                            <  1:1555896/140‑1 (MQ=255)

                                                                 aaaTCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATACAGta                                                                         >  1:1337590/1‑140 (MQ=255)

                                                                 aaaTCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATAAAAGCGGAAGCCGTCGGCATCGTATTACTCAATCCAGta                                                                         >  1:3206285/1‑140 (MQ=255)

                                                                  aaTCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGtat                                                                        <  2:792688/140‑1 (MQ=255)

                                                                   aTCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGtata                                                                       >  2:3718893/1‑140 (MQ=255)

                                                                   aTCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGgata                                                                       >  2:2841602/1‑140 (MQ=255)

                                                                        aCCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTaaa                                                                                                                              >  2:3875076/1‑80 (MQ=255)

                                                                        aCCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTaaa                                                                                                                              <  1:3875076/80‑1 (MQ=255)

                                                                            ttCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAAt                                                              <  1:2307932/140‑1 (MQ=255)

                                                                            ttCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAAt                                                              <  2:1794368/140‑1 (MQ=255)

                                                                              ctcCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATTCCAAGCAGATCAAAGCGGAAGCAGTCGGCATCGTATTCCTCAATCAAGTATATGACGCAAtac                                                            >  2:2457724/1‑138 (MQ=255)

                                                                              ctcCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATcc                                                            >  1:706636/1‑140 (MQ=255)

                                                                              ctcCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATcc                                                            >  1:82782/1‑140 (MQ=255)

                                                                              ctcCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATcc                                                            >  1:881552/1‑140 (MQ=255)

                                                                              ctcCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATcc                                                            >  2:240671/1‑140 (MQ=255)

                                                                              ctcCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCAAAAAAATCACGGTATAAATATATAAAAAGCACAGCAGATCAAACCGGAAGCCCTCGGCAGCGGATTGCTCAATCAACTATATGACCAAATca                                                            >  1:1599507/1‑139 (MQ=255)

                                                                                      aGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGGAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCt                                                                                   >  2:2094843/1‑109 (MQ=255)

                                                                                      aGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCt                                                                                   <  1:2080923/109‑1 (MQ=255)

                                                                                      aGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCt                                                                                   >  2:2080923/1‑109 (MQ=255)

                                                                                      aGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCt                                                                                   <  1:2094843/109‑1 (MQ=255)

                                                                                            agtCCAGGCTTGACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCt                                              <  1:2967687/138‑1 (MQ=255)

                                                                                                       tACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTc                                   >  2:2531988/1‑140 (MQ=255)

                                                                                                       tACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTc                                   >  1:3243312/1‑140 (MQ=255)

                                                                                                       tACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTc                                   >  2:3231191/1‑140 (MQ=255)

                                                                                                       tACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCAATGAATTTTCTTGCCAGCCGTc                                   >  1:3115835/1‑140 (MQ=255)

                                                                                                        aCCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATAACTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATGGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCt                                  >  2:3669642/1‑140 (MQ=255)

                                                                                                            cAGTTGCTTTTTCTTTCATATAAAGCCCTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCt                              >  2:1443482/1‑140 (MQ=255)

                                                                                                            cAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTACACTGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCt                              <  2:364489/140‑1 (MQ=255)

                                                                                                            cAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCt                              >  2:1932286/1‑140 (MQ=255)

                                                                                                              gTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGa                            >  2:285833/1‑140 (MQ=255)

                                                                                                               ttGCTTTTTCTTTCATTTAAAGCACTCTATCAATATATAAATTGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCACTATATGAAGCAATCCGCTATGAATTTTGTTGACATACGTCTTTATGaa                           >  1:1728851/1‑139 (MQ=255)

                                                                                                               ttGCTTTTTCTTTCATATAAAGCACTTTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTAGTCCTCAATCCAGTATATGTCGCAATCCGCTATGAATTTTCTTGCCCTCCGTCTTTCTGAc                           >  1:3740861/1‑140 (MQ=255)

                                                                                                               ttGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCACCATCCAGTATAAGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGAc                           >  2:898314/1‑140 (MQ=255)

                                                                                                                  cTTTTTCTTTCATATAAAGCACTGTATCAATCTCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGAcgcg                        <  1:2585735/140‑1 (MQ=255)

                                                                                                                  cTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGAcgcg                        <  1:1310068/140‑1 (MQ=255)

                                                                                                                  cTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGAcgcg                        <  1:3315378/140‑1 (MQ=255)

                                                                                                                  cTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGAcgcg                        <  1:3871497/140‑1 (MQ=255)

                                                                                                                  cTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTAGTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGAcgcg                        <  1:1061708/140‑1 (MQ=255)

                                                                                                                       tctttcATCTAAAGCACTGTATCACTATCTAAAAGGCCAATCATATGACAGCGGAAGGCGTCGGCATCGTATTCCTCAGTCCAGTATTTGACGCAATCCTCTATGAATTTTCTTGCCATCCGTCTTTCTGACGGTATGTc                   <  2:2358582/140‑1 (MQ=255)

                                                                                                                        ctttcATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCa                  <  1:1206689/140‑1 (MQ=255)

                                                                                                                           tcATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTg               >  2:2164572/1‑140 (MQ=255)

                                                                                                                             atatAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTGCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGcc             <  2:3472267/140‑1 (MQ=255)

                                                                                                                             atatAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGcc             <  1:3718893/140‑1 (MQ=255)

                                                                                                                               ataAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATTACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCaa           >  2:3470112/1‑140 (MQ=255)

                                                                                                                                 aaaGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACg         <  2:706636/140‑1 (MQ=255)

                                                                                                                                     cACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACGCCgg     <  1:2457724/140‑1 (MQ=255)

                                                                                                                                     cACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACGCCgg     <  1:146914/140‑1 (MQ=255)

                                                                                                                                        tGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACGCCGGTGc  >  1:476528/1‑140 (MQ=255)

                                                                                                                                        tGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACGCCGGTGc  >  1:3290475/1‑140 (MQ=255)

                                                                                                                                        tGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACGCCGGTGc  >  2:3218734/1‑140 (MQ=255)

                                                                                                                                        tGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACGCCGGTGc  >  2:2500892/1‑140 (MQ=255)

                                                                                                                                        tGTATCAATATCTAAAATGCCAAGCAGAACAAAGCGGAAGCCGTCGGCATCTTATTCCTCAATCCAGTATATGACGCAATCCGCTATCAATTTTCTAGCCATCCGTCTTTCTGACGCGATGTCATAGCCAACGCCGGTGc  >  1:3599640/1‑140 (MQ=255)

CGATGCCCGGCATTTTTGTAGCGTTCGCCAAGGTCGCTTTTTGTTCTTGCGGCAGAGGTGTCGCCAAATCCCACCCTTCTCCAAAAAGCAGGATTCCAGGCTTTACCGCAGTTGCTTTTTCTTTCATATAAAGCACTGTATCAATATCTAAAATGCCAAGCAGATCAAAGCGGAAGCCGTCGGCATCGTATTCCTCAATCCAGTATATGACGCAATCCGCTATGAATTTTCTTGCCATCCGTCTTTCTGACGCGATGTCATTGCCAACGCCGGTGC  >  NZ_CP034943/2864437‑2864712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`