Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 485,053 G→T 8.8% L445F (TTG→TTT kimA → potassium transporter KimA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943485,0530GT 8.8% 552.5 / 5.5 236L445F (TTG→TTTkimApotassium transporter KimA
Reads supporting (aligned to +/- strand):  ref base G (90/115);  new base T (28/2);  total (119/117)
Fisher's exact test for biased strand distribution p-value = 1.78e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

AAACCAAAAGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAA  >  NZ_CP034943/484916‑485182
                                                                                                                                         |                                                                                                                                 
      aaaGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGAGCCTGTTTGTGCCGAAGTTTAATGTTGTTTGGCGTGTGTTAATCTTTATGCCTATCGTCGTTTTGGTGttttt                                                                                                                           >  2:1344774/1‑140 (MQ=255)
       aaGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGtttttt                                                                                                                          >  1:4028375/1‑140 (MQ=255)
            tGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGGGTTAATCTTTATGCCTATCGTCGTTTTGGTGTTTTTTTCGAt                                                                                                                     >  1:69018/1‑140 (MQ=255)
              gATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTa                                                                                                                   >  2:3498168/1‑140 (MQ=255)
                tCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACTAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTaaa                                                                                                                 <  1:384301/140‑1 (MQ=255)
                tCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTaca                                                                                                                 <  2:3608414/140‑3 (MQ=255)
                 cGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTaaaa                                                                                                                >  2:3048750/1‑140 (MQ=255)
                   gAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGCTCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCGTTTAAAAAt                                                                                                              >  2:2546435/1‑140 (MQ=255)
                   gAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAAt                                                                                                              <  1:1061599/140‑1 (MQ=255)
                   gAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAAt                                                                                                              <  2:778502/140‑1 (MQ=255)
                   gAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAAt                                                                                                              <  2:110023/140‑1 (MQ=255)
                   gAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAAt                                                                                                              <  1:939829/140‑1 (MQ=255)
                   gAAAAATGCTGATCAACTCCTGCGGCGCGTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAAt                                                                                                              <  1:2423674/140‑1 (MQ=255)
                     aaaaTGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCa                                                                                                            <  2:2557119/140‑1 (MQ=255)
                     aaaaTGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTGTTGCTGTTTTTTTCGATTAAAAAtaa                                                                                                            >  2:788325/1‑138 (MQ=255)
                         tGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTGTGTTTTTTTGGATTAAAAAtaaaaaa                                                                                                        >  1:1049549/1‑134 (MQ=255)
                         tGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATCACTac                                                                                                        >  1:974903/1‑140 (MQ=255)
                          gCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTaca                                                                                                       <  1:1738772/140‑1 (MQ=255)
                              aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                   <  1:3485862/140‑1 (MQ=255)
                              aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGGTTTTTGCGATTAAAAAt                                                                                                              >  2:89175/1‑129 (MQ=255)
                              aTCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGCTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGc                                                                                                   <  2:4010826/140‑1 (MQ=255)
                                     ccTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGTCGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTGTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGt                                                                                            >  2:3786764/1‑140 (MQ=255)
                                        gcggcgCTTTGATTTGGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGGCTGTGTTAATCTTTATGCCGATCGTCGTTTTGCTGTTTTTTTCGTTTAAAAATCACTAAAAAGATGTCGGTGaa                                                                                         >  2:2299242/1‑140 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATTGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                         >  1:748890/1‑140 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTTCGATTAAAAATAACTACACAGCTGTAgggtaa                                                                                         >  1:3092836/1‑136 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                         <  1:321350/140‑1 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                         <  1:1620307/140‑1 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                         <  1:2832308/140‑1 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATAGTCGTTTTGCTGTTTTTTTCGTTTAAAAATAACTAAAAAGCTGTCGGTGaa                                                                                         >  2:3317534/1‑140 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCGATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGaa                                                                                         >  2:582430/1‑140 (MQ=255)
                                        gcggcgCTTTGATTTCGTTTATGGTTCGGTCGATCCGGTTTGTGACGAAGTTTAATTTTGTATGGCCTGTTTTAATCTTTATGCCTATAGTCGTTTTTCAGTTTTTTGCGGGTGAAAATAACAACACAGCAGTGGGTTaa                                                                                         >  1:1750499/1‑140 (MQ=255)
                                             gcTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCt                                                                                    <  1:545630/140‑1 (MQ=255)
                                              cTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTGTGTTTTTTGCGCTTAAAAAAAACAAAACACCTGTCGGTGAACAGCtt                                                                                   >  1:1342990/1‑140 (MQ=255)
                                              cTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCtt                                                                                   >  2:1508220/1‑140 (MQ=255)
                                                ttGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCg                                                                                 <  1:1169463/140‑1 (MQ=255)
                                                ttGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCg                                                                                 <  2:2233667/140‑1 (MQ=255)
                                                ttGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCg                                                                                 <  1:2744409/140‑1 (MQ=255)
                                                ttGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCg                                                                                 <  2:28683/140‑1 (MQ=255)
                                                 tGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGc                                                                                <  1:2507234/140‑1 (MQ=255)
                                                 tGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGc                                                                                <  1:2023024/140‑1 (MQ=255)
                                                     ttCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCg                                                                            <  2:1289813/140‑1 (MQ=255)
                                                       cGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTa                                                                          <  1:2856007/140‑1 (MQ=255)
                                                            aTGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACaa                                                                     <  1:3698180/140‑1 (MQ=255)
                                                                    gTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCag                                                             <  2:654316/140‑1 (MQ=255)
                                                                          ccTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACAAAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGaaa                                                       >  2:3847811/1‑140 (MQ=255)
                                                                          ccTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTGTTTCTGTTTTTTTCGATTAAAAATCAATAAAAAGATTTCGGTGAACAGCTTCGCATCGTAGAAAAACAGCCAGAAGaaa                                                       >  2:2938870/1‑140 (MQ=255)
                                                                              tttGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCaa                                                   <  2:3344520/140‑1 (MQ=255)
                                                                               ttGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACACAGCTTTCGGTGAACAGCTTCGCATCGTAGACAAATAGCCAGAAGAAATCaaa                                                  >  2:297755/1‑140 (MQ=255)
                                                                               ttGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGGTGTTTTTTGCGATTATAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATAGTAGAAAAATATCCAGAAGAAATCaaa                                                  >  2:1682206/1‑140 (MQ=255)
                                                                                  tgACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGc                                               <  1:3065953/140‑1 (MQ=255)
                                                                                   gACGAAGTTTACTGTTGGATGGCCTGTGTTCATCTTTATGCCTACCGTCGTTCTGCTGTTTTTTGCGACTAAAAATCACTCCCCCGCTGTCGGTGAACAGCTTCGCATCGTAGACAAATAGCAAGAAGAAATCAAAGGCa                                              <  1:503401/140‑1 (MQ=255)
                                                                                   gACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCa                                              <  2:4028375/140‑1 (MQ=255)
                                                                                   gACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCa                                              <  1:1835448/140‑1 (MQ=255)
                                                                                   gACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCa                                              <  1:3512849/140‑1 (MQ=255)
                                                                                   gACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTGATGCCTAGCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCa                                              <  2:2731486/140‑1 (MQ=255)
                                                                                    aCGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCAc                                             <  1:1093941/140‑1 (MQ=255)
                                                                                    aCGAAGTTAAATGTTGGATGGACTGTGTTAATCTTTATGCCGCTCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCGTCGCCTCGTAGACAAAGAGCCAGCAGAAATCAAAGCCAc                                             <  2:341718/140‑1 (MQ=255)
                                                                                      gAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTCTCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACgg                                           <  2:1941805/140‑1 (MQ=255)
                                                                                        aGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGtt                                         <  1:1805113/140‑1 (MQ=255)
                                                                                        aGTTTAATGTTGTATGGCCTGTGTTAATCTGTATGCCTATCTTCGTTTTGATGTTTTTTGCTATTAAAAAAAACATCACATCTGTCGGTGAACACCTTCGCATCGGAGACAAAGAGCCACAAGAAATCAAAGTCCCGGtt                                         >  1:3956925/1‑140 (MQ=255)
                                                                                              aTGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGAtt                                   <  1:3815040/140‑1 (MQ=255)
                                                                                              aTGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGAtt                                   <  1:890048/140‑1 (MQ=255)
                                                                                               tgttgtATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATtg                                  <  1:3151521/140‑1 (MQ=255)
                                                                                                gttgtATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATtgt                                 <  2:921430/140‑1 (MQ=255)
                                                                                                gttgtATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATtgt                                 <  2:2849945/140‑1 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGGTGTGATTGTGCCt                             >  2:1052775/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTAGGTGAACAGCTTCGCATCGTACAAAAAGAGCCAGAAGAAAACAAATCCACGGTTGTTATTGTGCCt                             >  2:2708272/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACACACCTGTCGGTGAACAGCATAGCATCGTAGACAAAGATCCCCAAGAAAACAAACGCACGGTTGTTATTGTGCCt                             >  1:789325/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCAATACACAGCTGTCGGTTAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCt                             >  2:1319082/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAAAAACAAAACACCTGTAGGTGAACACCTTAGCATAGTAGACAAAAAGCCACAAAAAAAAAAAAACACCCTTGTTATTGTGACt                             >  1:1387809/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTTTTTTTTGCGATTAAAAAACACTACAAAGCAGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCt                             >  1:2779747/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAATAAATCAAAGGCACGGTTGTGATTGTGCCt                             >  2:2625120/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCt                             >  1:989051/1‑140 (MQ=255)
                                                                                                    tATGGCCTGTGTTAATCTTTATGCCTATCGTCGTGTTGCTGTTTTTTGCGATTAAAAATCACGACACAGCTGTCCGTGAACACCTTAGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACCGTTTTGATTGTGCCt                             >  2:1808039/1‑140 (MQ=255)
                                                                                                          cggtgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                       <  2:3284141/138‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                       <  2:52109/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                       <  1:2898960/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                       <  1:3039370/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                       <  2:1419389/140‑1 (MQ=255)
                                                                                                          cTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGcc                       <  1:1508220/140‑1 (MQ=255)
                                                                                                           tgtgTTAATCTTTATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAAACACTAAAAACCTGTCGGTGAACAGCTTAGCATCGGATAAAAAGAGCCAGAAGAAAACAAAGGCACGGTTTTTATTGTGCCTGTAGCCg                      >  1:1550076/1‑140 (MQ=255)
                                                                                                           tgtgTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACAAAACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGATCCAGAAGAAATCAAAAGCACGGTTTTTATTGTGCCTGTAGCCg                      >  1:1952494/1‑140 (MQ=255)
                                                                                                             tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCTCATCGTATAAAAATAGCCAGAAGAAATCAAAAGCACGGTTGTTATTGTGCCTTTAGCAGgt                    >  2:2643879/1‑140 (MQ=255)
                                                                                                             tgtTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGgt                    <  2:69018/140‑1 (MQ=255)
                                                                                                             tgtTAATCTTTATGCCTATCGTCGTTTTGCGGTTTTTTGCGATTAAAAATCACTACAAAGCAGTCGGTGAACAGCTTCGCAACCGAGACAAAGATCCAGAAGAAATCAAAAGAACCGTTGTGATTGTGCCTGTAGCCCgt                    >  1:2327621/1‑140 (MQ=255)
                                                                                                                  aTCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGGTGTGATTGTGCCTGTAGCCGGTGTtac               >  2:2153731/1‑140 (MQ=255)
                                                                                                                  aTCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAAAAGCTTCGCATCGTAGACAAAGAGCCAGAATAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTtac               >  2:83324/1‑140 (MQ=255)
                                                                                                                  aTCTTTATGCCTATCGTCGTTTTGCAGTTTTTTTCGATTAAAAATAACTACACAGCTTTCGGTGAACATGTTATCATGGTAGACCAAGAGCCCGAATAAATAAAAGGCAAGGGTGTGATTGTGGCTGTAGCCGGTGttta               >  2:1189272/1‑138 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAATCACTAAACACCAGTCCGTTAACAGCATCGCATCCTAGACAAAGAGCCACAAGAAAAAAAAGGCACGGTTGTGCTGTTGCCTGGAGCCGGTGTTAATGCcg          >  1:2623960/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTTCTGTTTTTTTCGATTAAAAATAACTAAACAGCTGTCGGTGAACACCTTCGCATCCTAGACAAAGAGCCAGAAGAAATAAAAGGCACCGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg          >  1:2542245/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTTCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCGTCGCATCGTAGACAAATAGCCAGAAGAAATAAAAGGCACCGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg          >  1:2123507/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTTTGATTTTGGCGGTAGCCGGTGTTACTAAcg          >  1:718498/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGGCTGTAGCCGGTGTTACTAccc          >  2:3111260/1‑139 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg          <  1:124897/140‑1 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGGAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTTTGCCTGTAGCCGGTGTTACTACcg          >  1:869554/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATAACTACACAGCTGTAGGTGAACAGCTTCGCATCGTAGACAAAGACCCACAAGAAATCAAAGGCACCGTTGTGATTGTGCCAGTAGCCGGTGTTACTAccc          >  2:2239453/1‑139 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAAACACTACACAGCTGTCGGTGAACAGCTTCTCATCGTAGACAAACAGCCACAAGAAATCAAAGGCACGGTTGTGATTTTGCCTGTATCCGGTGTtactaaag          >  1:3280726/1‑137 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTGTTGCTGTTTTTTGCGATTAAAAATCCCTACACAGCTGGCGGTGAACAGCGTCGCATCGGAGACACAGAGCCAGGCGAAATCACAGGCACGGGTGTGGTTGTGCCTGTAGCCCGTGTTTCTACcg          >  1:560893/1‑140 (MQ=255)
                                                                                                                       tATGCCTATCGTCGTGTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACcg          >  1:2620242/1‑140 (MQ=255)
                                                                                                                               tcgtcgTTTTGCTGTTTTTTGCGATTAAAAATCACTACAAAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCGGTAGACGGTGTTACTAGCGTCGTGcca  >  2:2633007/1‑138 (MQ=255)
                                                                                                                                         |                                                                                                                                 
AAACCAAAAGGGTGGATCGGAAAAATGCTGATCAACTCCTGCGGCGCTTTGATTTCGTTTATGGTTCTGTCGATCCTGTTTGTGACGAAGTTTAATGTTGTATGGCCTGTGTTAATCTTTATGCCTATCGTCGTTTTGCTGTTTTTTGCGATTAAAAATCACTACACAGCTGTCGGTGAACAGCTTCGCATCGTAGACAAAGAGCCAGAAGAAATCAAAGGCACGGTTGTGATTGTGCCTGTAGCCGGTGTTACTACCGTCGTGCAA  >  NZ_CP034943/484916‑485182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: