Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,294,907 T→A 6.0% D288V (GAT→GTT)  eno ← phosphopyruvate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,294,9070TA 6.0% 763.7 / 3.2 284D288V (GAT→GTT) enophosphopyruvate hydratase
Reads supporting (aligned to +/- strand):  ref base T (120/139);  new base A (21/2);  total (143/141)
Fisher's exact test for biased strand distribution p-value = 3.40e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

TGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACTCAGAAGATGCAGCATC  >  NZ_CP034943/3294770‑3295046
                                                                                                                                         |                                                                                                                                           
tGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCt                                                                                                                                           <  1:648530/140‑1 (MQ=255)
tGCCTACGCAGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCt                                                                                                                                           <  1:1009103/140‑1 (MQ=255)
 gCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCtt                                                                                                                                          <  2:2912661/140‑1 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAATAAGCTTGTGGCCTTCCCAGTCGTTTTCGTAAATTGCATCtta                                                                                                                                         >  2:503378/1‑139 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAATTCCATATTc                                                                                                                                         >  2:3012353/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAATTCCAACTTc                                                                                                                                         >  1:3942820/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                                         >  1:1736812/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                                         >  2:1810555/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                                         >  2:1660750/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                                         >  2:2509515/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                                         >  1:219768/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                                         >  1:1486213/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                                         >  2:388539/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                                         >  2:3199117/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCAACTTc                                                                                                                                         >  1:2113923/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCAATCTTc                                                                                                                                         >  1:944244/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTAGTCAAGTCCATCTTc                                                                                                                                         >  1:2962179/1‑140 (MQ=255)
  ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTAGTCAAGTACAACTTc                                                                                                                                         >  1:1922097/1‑140 (MQ=255)
  ccTACGCCGTTGTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATcgtg                                                                                                                                         >  2:2516618/1‑137 (MQ=255)
  ccTACGCCGTGTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTAGTCAAGTCCAGCttg                                                                                                                                         >  1:2252362/1‑139 (MQ=255)
  ccTACGCCGTGTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTGTTCGTCCAGTCAATCTTc                                                                                                                                         >  2:634993/1‑140 (MQ=255)
       gCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTGCATGTTCGATag                                                                                                                                    >  2:3920488/1‑140 (MQ=255)
       gCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATat                                                                                                                                    >  2:1359974/1‑139 (MQ=255)
       gCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATag                                                                                                                                    <  1:2405543/140‑1 (MQ=255)
       gCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCAACTTCGATag                                                                                                                                    >  1:389894/1‑140 (MQ=255)
       gCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTAGTCAAGTACATCATGGATag                                                                                                                                    >  1:1300934/1‑140 (MQ=255)
           tttttGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAgat                                                                                                                                >  2:1970566/1‑140 (MQ=255)
            ttttGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAgaga                                                                                                                                 <  1:730426/138‑1 (MQ=255)
             tttGATACCTTCAGCAAGTTTTTTCGTGTTACTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCATTCGTTTTCGTCAATTCCATCTTCGATagataaga                                                                                                                              >  2:3849011/1‑135 (MQ=255)
               tGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGAtt                                                                                                                            <  2:3091002/140‑1 (MQ=255)
               tGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGAtt                                                                                                                            <  2:589956/140‑1 (MQ=255)
                  tACCTTCAGCAAGTTTGTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTggg                                                                                                                         <  2:3980284/140‑1 (MQ=255)
                       tCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCCAGTCCAACTTCGATAGAGATGATTTGGTAttt                                                                                                                    >  2:2772516/1‑140 (MQ=255)
                        cAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTGCGATAGAGATGATTTGGTATTTa                                                                                                                   >  2:1297775/1‑140 (MQ=255)
                         aGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGa                                                                                                                                       >  2:2990521/1‑119 (MQ=255)
                                   ttCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGGCAACTCCATCTTCGATAGAGATGATTTGGTATTTATAAACCAtttg                                                                                                        >  2:3607437/1‑139 (MQ=255)
                                          ttGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTAc                                                                                                 <  1:1522949/140‑1 (MQ=255)
                                            gTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCa                                                                                               <  1:361948/140‑1 (MQ=255)
                                                cGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTACGTACCAGTCa                                                                                           <  1:2989057/140‑1 (MQ=255)
                                                     agGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCAt                                                                                      <  2:1139835/140‑1 (MQ=255)
                                                          atcaCCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTGAAGTCCATCTTCGGTATTGATGTTTGGGTATTTATAAACCAGTTGTTAGGTCCAGTGAACCATTTgag                                                                                 >  1:2316031/1‑140 (MQ=255)
                                                            caCCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTcagca                                                                               <  2:2158608/140‑1 (MQ=255)
                                                               caacaaGCTGAACTTTTTTGCCAGGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGAt                                                                            <  2:2331584/140‑1 (MQ=255)
                                                               caacaaGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGAt                                                                            <  2:1446059/140‑1 (MQ=255)
                                                                 acaaGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATTATTGGGTATTTAGAAACCATTTGTTGGTTCCAGTCAACCATTTCATGAGATGt                                                                          >  1:2512128/1‑140 (MQ=255)
                                                                 acaaGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTTGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCAATTAAGCAGAttt                                                                          >  1:850573/1‑138 (MQ=255)
                                                                 acaaGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGt                                                                          <  2:3769112/140‑1 (MQ=255)
                                                                 acaaGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGAATATTGGGTATTTAGAAACCATTTCTTAGTTCCAGTCAACCAATTAAGCAGAttt                                                                          >  2:2254764/1‑138 (MQ=255)
                                                                    aGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGa                                                                                                                                       <  2:796558/76‑1 (MQ=255)
                                                                      cTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGTTTGGGTATTTATAAACCATTTCTTCGTTCCAGTCAACCATTTAAGCAGATGTTTTca                                                                     >  2:3379086/1‑140 (MQ=255)
                                                                         aaCTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCCATAGAGATCATTGGGTATTTAGAAACCAATTATTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACaa                                                                  >  2:1091413/1‑140 (MQ=255)
                                                                                    cAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCca                                                       <  2:1724325/140‑1 (MQ=255)
                                                                                    cAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGGCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCca                                                       <  2:1219189/140‑1 (MQ=255)
                                                                                      aGACGCTCATTAAGTAGCTTGTGTGATTCCCAGTCGTTTTCGTCAAGTCCATGTTCGATAGAGATGATTGGGTATTTAGAAACCATTTATTCGTACCAGTCAACCATTTCAGAAGATGTTTTCACAACGCCTTCGCcaga                                                     <  2:416519/140‑1 (MQ=255)
                                                                                      aGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCcaga                                                     <  2:2061602/140‑1 (MQ=255)
                                                                                          gCTCAGTAAGAAGCTTGTGGCCTTGCCAGTCGTTTTCGTCAAGTCCATCTTCTATATAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCcagacaga                                                 <  1:3138522/140‑1 (MQ=255)
                                                                                          gCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCcagacaga                                                 <  2:1486213/140‑1 (MQ=255)
                                                                                          gCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCcagacaga                                                 <  1:3964927/140‑1 (MQ=255)
                                                                                          gCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCcagacaga                                                 <  1:2458811/140‑1 (MQ=255)
                                                                                           cTCAGTAAGAAGGTTGTGGCCTTCCCAGTCGTGTTCTTCAAGTCCATCTTCGATAGAGATGATTTGGTATTGAGTCACCATTTCTTCTTACCAGTCAACAATTTAAGGCGATGTTTTAACAACGCCTTCGCCAGACAGAt                                                <  2:2556515/140‑1 (MQ=255)
                                                                                           cTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGAt                                                <  2:543470/140‑1 (MQ=255)
                                                                                           cTCAGTAAGAAGCTTGTGCCCTTCCCAGTCGTGTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGAt                                                <  2:268095/140‑1 (MQ=255)
                                                                                             cAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTGCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTTGTAACCATTTCTTCGGAACAGTCCACCATTTCAGCAGAAGTTTTAACAACCCCGTCGCCCGACCGAggg                                              >  1:3342260/1‑140 (MQ=255)
                                                                                                tAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTACAAACCATTTATTCGTACCAGTCAACCATTTCACCAGATGTTTTAACAACGCCTTCGCCAGACAGATGGTAt                                           <  1:2598912/140‑1 (MQ=255)
                                                                                                     agCTTGTGGCCTTCCCCGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGcc                                      <  1:1040200/140‑1 (MQ=255)
                                                                                                     agCGTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGcc                                      <  1:3040293/140‑1 (MQ=255)
                                                                                                      gCTTGTGGCCTTCCCAGTCGTGTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGGATTTAGAAACCATTTCTTCGTGCCAGTCAACCATTTCAGCAGATGTTTTGACAACGCCTTCGCCAGACAGATGGTATTTGGCg                                     >  1:2724212/1‑140 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTGTTCGTTCCAGTCAACCATTTCACCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtcgt                                 >  1:3421485/1‑138 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtctt                                 >  2:3153686/1‑140 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtctt                                 >  2:1348826/1‑140 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCAATTCAGCAGATGTTTTAACAAAGCCATCGCCAGACACATGGTATTTGCCGtcat                                 >  2:3177964/1‑138 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCGTCGTACCAGTCAACCATTTAAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtcgt                                 >  2:1163873/1‑138 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCAATTCTTAGTACCAGTCCAACATTTCAGCAGATGTTTTCACAAAGCCTTCGCCAGACCGATGGTATTTTACGtctt                                 >  1:2923412/1‑140 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCAACGTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCAATTAAGCAGATGTTTTCACAACCCCATCGCCAGACAGATGGTATTTGCCCtctt                                 >  1:612086/1‑140 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAAGTACATCTTCGATAGAGATGATTGGGTTTTTAGAAACCATTTGTTGGTACCAGTCAACCATTTCATCAGATGTTTTCAAAACGCCTTCGCCACACAGATGGTATTTGCCGtgtt                                 >  1:3827160/1‑140 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGTCAACTCCAACGTCGATATAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCACCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtctt                                 >  2:2815843/1‑140 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTTTCGGCAAGTCCATCTTCGATAGAGATTATTGGGGATTTAGAAACCAATTCTTCGTACCAGTCCACCAATTAAGCAGATGTTTTAAAAACCCCGTCGCCCCACACATGGTATTTTCCGtctg                                 >  1:1152059/1‑139 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTTGTCGTCCAGTCCATCTTCGTTAGTGTTTATTGGGGATTTAGAAACCATTTCGTCGTACCAGTCAACCAGTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCggcgg                                 >  1:2797308/1‑136 (MQ=255)
                                                                                                          gtgGCCTTCCCAGTCGTGTTCGTCAAGTCCATCTTCGATAGATATTATTGGGTATTTAGAAACCATTTATTAGTACCAGTCAACCATTTCAGCAGATGTTTTAAAAACGCCTTCGCCAGACAGATGGTATTTTGCGtcgt                                 >  1:506954/1‑138 (MQ=255)
                                                                                                            ggCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtcttct                               <  2:95430/140‑1 (MQ=255)
                                                                                                            ggCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtcttct                               <  1:1109949/140‑1 (MQ=255)
                                                                                                            ggCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtcttct                               <  2:1112362/140‑1 (MQ=255)
                                                                                                             gCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTcttctt                              <  1:3684676/140‑1 (MQ=255)
                                                                                                             gCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTcttctt                              <  2:862270/140‑1 (MQ=255)
                                                                                                             gCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTcttctt                              <  2:1736812/140‑1 (MQ=255)
                                                                                                             gCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTcttctt                              <  1:3246211/140‑1 (MQ=255)
                                                                                                             gCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTcttctt                              <  2:3226744/140‑1 (MQ=255)
                                                                                                               cTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTttg                            <  2:3252508/140‑1 (MQ=255)
                                                                                                                ttCCCAGTCGTGTTCGTCAAGTCCAACTTCGATAGAGATGATTGGGTCTTTAGAAACCATTTCTTCGTACCAGTCAAACAATTAAGCACATTGTTTCACAAAGCCATCGCCAGACAGATGGTATTTGCCGTCGTCTgtgt                           >  2:703915/1‑140 (MQ=255)
                                                                                                                 tCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTATTAACCATTTAGTCGTACCAGTCAACCATTTCAGCACATGTTTTCACAACGCCGTCGCCCGACACATGGGATTTTCCGTCTTCTttgtt                          >  2:2529929/1‑140 (MQ=255)
                                                                                                                    cAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTa                       <  1:1353681/140‑1 (MQ=255)
                                                                                                                     aGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAg                      <  1:397221/140‑1 (MQ=255)
                                                                                                                     aGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAg                      <  1:3203679/140‑1 (MQ=255)
                                                                                                                            ttCGTCAAGTCCATCTTCGATAGAGATTATTTGCTATTTAGAAACCATTTCTTAGTACCAGTCAACCATTTCAGCAGATGTTTTAAAAAAGCCATCGCCACACAGATGGTATTTGCCGTCTTCTTTGTTGTAGaaaacag               >  2:343042/1‑135 (MQ=255)
                                                                                                                            ttCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATTTTTTCACAACCCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACTCag               >  2:1017389/1‑140 (MQ=255)
                                                                                                                            ttCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCGTCGCCAGACAGATGGTATTTGCCGTCTTCTTTTTTGTAGAACTCag               >  2:3712850/1‑140 (MQ=255)
                                                                                                                            ttCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTAAGCACATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACtaat               >  2:2428213/1‑137 (MQ=255)
                                                                                                                            ttCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTAGTACCACTCAACCATTTCAGCAGATGTTTTAACAACGCCTTCGCCAGACAGATGGTATTTTCCGTCTTCTTTGTTGTAGAACTCag               >  2:1301876/1‑140 (MQ=255)
                                                                                                                             tCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACTCaga              <  2:390417/140‑1 (MQ=255)
                                                                                                                                         tCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACTCAGAAGATGCAGCATc  >  2:1849815/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACTCAGAAGATGCAGCATC  >  NZ_CP034943/3294770‑3295046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: