Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,349,666 T→G 6.6% intergenic (+1/+26) EO946_RS17305 → / ← padC hypothetical protein/phenolic acid decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,349,6660TG 6.6% 750.2 / 9.6 330intergenic (+1/+26)EO946_RS17305/padChypothetical protein/phenolic acid decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (193/101);  new base G (14/20);  total (207/123)
Fisher's exact test for biased strand distribution p-value = 7.85e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

TATTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAG  >  NZ_CP034943/3349527‑3349804
                                                                                                                                           |                                                                                                                                          
    ttGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATtctc                                                                                                                                        >  1:814354/1‑140 (MQ=255)
    ttGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATtctc                                                                                                                                        >  1:2433374/1‑140 (MQ=255)
         ggCCGTGTTATGTTTACGGGACACAGCCTATTACAAGCCTATGCTCTGGACTGGCATAATACTGTTTTTCGCGGGAATTATGTTTTTTCCAGCATACTAAGCAAAAAGAAAACACTGGAACGAGAATTGATTCTATTTTc                                                                                                                                   >  2:3863958/1‑140 (MQ=255)
                ttATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGttt                                                                                                                            >  2:2868083/1‑140 (MQ=255)
                     tttACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATg                                                                                                                       >  2:108341/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGACTTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGGTTTTATGttt                                                                                                                    >  1:1726166/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATTCTCTTTTCCGGGTTTTTAGGttt                                                                                                                    >  1:2108972/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGttt                                                                                                                    >  1:450227/1‑140 (MQ=255)
                        aCGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTGTTCCGGGTTTTTATGttt                                                                                                                    >  1:3275186/1‑140 (MQ=255)
                          gggACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTttat                                                                                                                  >  2:1644591/1‑140 (MQ=255)
                               acaGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATaa                                                                                                             >  1:3865917/1‑140 (MQ=255)
                                 aGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAAtt                                                                                                           >  1:517074/1‑140 (MQ=255)
                                     tagtACCAGCCTATGCGCTGGACTGGCATCATACTCTTTTTCGCGGGAATTATGATGCTTCCCGCATACTCTGCTAACAGAAAACCCGGGAAAGAGAACTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:3019144/137‑1 (MQ=255)
                                     tATTACCAGCCTATTCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGACGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:2054807/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGTCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCAGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:3273297/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCTGTAATTATGATGGTTCCCGCATACTAAGCAAAAAGAAAAACCGTGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATGTTcc                                                                                                       <  2:511234/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:3771958/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:2919175/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  1:2957062/140‑1 (MQ=255)
                                     tATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGAGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTGTcc                                                                                                       <  2:2512350/140‑1 (MQ=255)
                                      aTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:1381298/139‑1 (MQ=255)
                                      aTTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGGTTTTATGTTTATTATAATTTTcc                                                                                                       >  1:1381298/1‑139 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:3007574/140‑1 (MQ=255)
                                       ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCACATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcg                                                                                                     <  1:3242420/140‑1 (MQ=255)
                                           cAGCCTATGCGCTGGACTCGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAATGAAAACCCGGGAAAGAGAAGTGATTGTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCcgcgcg                                                                                                 >  2:870470/1‑140 (MQ=255)
                                            aGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGt                                                                                                >  1:2275164/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATAGTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGGTTATTATAATTTTCCCGCGCGta                                                                                               >  1:1882853/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGACAGAGAAGTGATTCGCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:3845197/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCg                                                                                                                                 <  2:802867/106‑1 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       <  2:2363769/132‑1 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       >  1:1047237/1‑132 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTcc                                                                                                       >  1:2206733/1‑132 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:3229423/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:3888545/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:2432607/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:941442/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:1457065/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:2818591/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:1920236/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  2:3584328/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTGTCCCGCGCGta                                                                                               >  2:2670038/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTGTTCCGGGTTTTTATTTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:2779786/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTGTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:916149/1‑140 (MQ=255)
                                             gCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTGTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               >  1:2532755/1‑140 (MQ=255)
                                               cTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGACAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGta                                                                                               <  2:3320781/138‑1 (MQ=255)
                                                 aTGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATc                                                                                           >  2:1959391/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGACTTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGGTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:2855910/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  2:259396/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:1864452/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:3881526/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:3255576/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTGTTTATGTTTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:3343269/1‑140 (MQ=255)
                                                  tGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCAGACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTGTATTATAATTTTCCCGCGCGTATATCg                                                                                          >  1:268143/1‑140 (MQ=255)
                                                     gcTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCt                                                                                       <  2:1834339/140‑1 (MQ=255)
                                                        ggACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGttttt                                                                                                                          <  2:3150584/102‑1 (MQ=255)
                                                        ggACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTc                                                                                    >  2:1681145/1‑140 (MQ=255)
                                                        ggACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTc                                                                                    <  2:2969611/140‑1 (MQ=255)
                                                         gACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCa                                                                                   >  1:2608335/1‑140 (MQ=255)
                                                           cTGGAATCATACTGTGTTACGCGGGAATTATGATGGTTCCCGCATACTCAGCACACACAAACCCCGGGAAAGACAAGCGACTCTCTTGTCCGGGTTTTTATGTTTATTATAAAGTCCCCGCGCGTACATCGTCTCTCAtt                                                                                 <  2:1288756/140‑1 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 >  1:2710227/1‑137 (MQ=255)
                                                              gCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCt                                                                              <  1:3467874/140‑1 (MQ=255)
                                                                   atACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTCTATCGTCTGTCATTCCTTCATa                                                                         >  1:3302849/1‑140 (MQ=255)
                                                                     acgcttttgCGCGGGAATAATGATGGTTCCCGCATACCCGGCAAAAAGAAAACCCGGGAAAGAGACGTGATTCTCTTTTCCGGGGGTGTAAGGTTATGCTAATTGTGCCGCGCGTATCTCGTCTGTCCTTCGTTCATTAg                                                                       >  2:406301/5‑140 (MQ=255)
                                                                     aCTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAg                                                                       <  1:3646893/140‑1 (MQ=255)
                                                                     aCTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTAGATCGTCTGTCATTCCTTCATAAg                                                                       <  1:1229117/140‑1 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGCTTCTCTTTTCCGGGTTTGTAGGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                    >  2:1566678/1‑140 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAg                                                                    >  1:2353814/1‑140 (MQ=255)
                                                                        gTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCAtt                                                                                 <  1:3569156/127‑1 (MQ=255)
                                                                         tttttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGc                                                                   >  2:148835/1‑140 (MQ=255)
                                                                           tttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCtt                                                                 <  2:3547731/140‑1 (MQ=255)
                                                                            ttCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGGTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATATGGAGCttt                                                                >  1:2023908/1‑140 (MQ=255)
                                                                             tCGCGGGAATAATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGACTCTCTTTTCCGGGTTTTTATGATTATTATAATTTTCCCGCGCGGCTATCGTCTGTCAGTCCTTCATAAGGAGCTTTc                                                               <  2:445058/140‑1 (MQ=255)
                                                                              cgcgGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGGTTGTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCg                                                              >  1:1508271/1‑140 (MQ=255)
                                                                                            tgGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCg                                                <  2:3169041/140‑1 (MQ=255)
                                                                                            tgGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCg                                                <  1:1943633/140‑1 (MQ=255)
                                                                                              gTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGGTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGtt                                              >  2:1136761/1‑140 (MQ=255)
                                                                                                 cccGCATACTCAGCAAAATGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                           >  1:677918/1‑140 (MQ=255)
                                                                                                 cccGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTc                                           >  1:835269/1‑140 (MQ=255)
                                                                                                   cGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGa                                         <  1:1634793/140‑1 (MQ=255)
                                                                                                            aGCAAAATGAAAACCCGGGCAAGAGAAGTGATTCTCGGGTCCGGGTTTTTCTGTTTATTATACTTTTCCCGCGCGTCTCTCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGGTGTCGACTCCCTCAt                                >  2:3433937/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  2:1740310/1‑140 (MQ=255)
                                                                                                            aGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAt                                >  2:3721064/1‑140 (MQ=255)
                                                                                                              cAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCAttt                              >  2:353339/1‑140 (MQ=255)
                                                                                                                    gAAAACCCGGGCAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGa                        <  1:1278924/140‑1 (MQ=255)
                                                                                                                    gAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGa                        <  1:3148965/140‑1 (MQ=255)
                                                                                                                    gAAAACCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGa                        <  1:431837/140‑1 (MQ=255)
                                                                                                                       aaCCCGGGAAAGAGAAGTGAGTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAAGTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAg                     <  1:2071985/140‑1 (MQ=255)
                                                                                                                             ggAAAGAGAAGTGATTGTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTGCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAAtc               >  1:1141355/1‑140 (MQ=255)
                                                                                                                                  gagaAGTGATCCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGc          <  2:677918/140‑1 (MQ=255)
                                                                                                                                   agaAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCa         <  1:1820629/140‑1 (MQ=255)
                                                                                                                                    gaAGTGATTCTCTTTTCCGGGTTTGTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATAACTTCTTCTTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCaa        >  2:2628625/1‑140 (MQ=255)
                                                                                                                                       gTGATTCTCTTTTCCGGTTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTGATTCCTTCATAATGAGCTTTCGAAATTACTTCTTCGTTGTAGACTCAGTTATTTTTAATAAAGGTAATCTcctctattt     <  2:227838/140‑8 (MQ=255)
                                                                                                                                       gTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCTCGTATATCTTCTGTCATTCCTTCATAATGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCTGTCATTTTTAAGAAAGGTAATCTcctctattt     <  2:3236248/140‑8 (MQ=255)
                                                                                                                                       gTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAAtt     <  2:3275186/140‑1 (MQ=255)
                                                                                                                                       gTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAAtt     <  2:2108972/140‑1 (MQ=255)
                                                                                                                                       gTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAAtt     <  2:920349/140‑1 (MQ=255)
                                                                                                                                        ttaTTGTCTTTTCCGTGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTc    <  2:2779786/138‑1 (MQ=255)
                                                                                                                                        tGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTc    >  2:2183480/1‑140 (MQ=255)
                                                                                                                                        tGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTc    >  2:1215244/1‑140 (MQ=255)
                                                                                                                                          aTTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAg  >  1:3402208/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TATTTTGTCGGCCGTGTTATGTTTACGGGACACAGCCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTCGCGGGAATTATGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGGGAAAGAGAAGTGATTCTCTTTTCCGGGTTTTTATGTTTATTATAATTTTCCCGCGCGTATATCGTCTGTCATTCCTTCATAAGGAGCTTTCGAAATTACTTCTTCGTTGTCGACTCCCTCATTTTTCAGAAAGGTAATCTCCGCAAATTCAG  >  NZ_CP034943/3349527‑3349804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: