Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,995 C→A 9.1% V197V (GTG→GTT degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9950CA 9.1% 752.8 / 10.8 304V197V (GTG→GTTdegUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base C (75/185);  new base A (39/2);  total (117/187)
Fisher's exact test for biased strand distribution p-value = 1.24e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

AAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTG  >  NZ_CP034943/3446858‑3447132
                                                                                                                                         |                                                                                                                                         
aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                                         <  1:3913611/140‑1 (MQ=255)
             aGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTg                                                                                                                            <  2:3883651/140‑1 (MQ=255)
               tAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTAACGGTTTTCTCACTGAt                                                                                                                          >  1:3141208/1‑140 (MQ=255)
                aaaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAAGGTTTTCTCACTGATa                                                                                                                         <  2:1329057/140‑1 (MQ=255)
                  aaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATaaa                                                                                                                       <  1:2458428/140‑1 (MQ=255)
                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGGTCACATGGTTTTTCACGGTTTTCTGCGTGATAAACa                                                                                                                     >  2:3280478/1‑140 (MQ=255)
                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                                                     >  2:2481732/1‑140 (MQ=255)
                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAATATTGCTCCCATTGTTTTTAACGGTTTTATCAAGGATaaaaa                                                                                                                     >  1:1107679/1‑138 (MQ=255)
                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATaaaaa                                                                                                                     >  2:622727/1‑138 (MQ=255)
                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGAGTCCGGTCGTTTTCATTAATTTTTTGCAAAATTTTACTCACAAGGTTTTTCACCGTTTTATCACTCATaaaaa                                                                                                                     >  1:2968664/1‑138 (MQ=255)
                       aaGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAAATGCTCACATTGTTTTTCACCGTTTTATAACTTATaaaaaaag                                                                                                                  >  2:3638791/1‑138 (MQ=255)
                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                 <  2:456079/140‑1 (MQ=255)
                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                 <  1:1870400/140‑1 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTAAAAATATTGATCACATGGTTTTTCACGGTTTTATCACTGATAAAAAATTAt                                                                                                                >  2:3149112/1‑140 (MQ=255)
                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTGCAAAATATTTCTCACAATGTTTTTCAAGGTTTTGTAACTGATAAAAAAAGAt                                                                                                                >  1:2877715/1‑140 (MQ=255)
                          tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACCACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                               <  1:3830671/140‑1 (MQ=255)
                          tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGTGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                               <  1:1182892/140‑1 (MQ=255)
                          tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                               <  1:3280478/140‑1 (MQ=255)
                          tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                               <  1:2580775/140‑1 (MQ=255)
                          tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                               <  1:775836/140‑1 (MQ=255)
                               tattatACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAATATTGCTCACATTGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTCAACa                                                                                                          >  2:2710962/1‑140 (MQ=255)
                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAATTCATTTTTTGCAAAATATTTGTGAGATGGTTTTTCACGGTTTTGTACCTGATAAAAAATTCTTCACCAAttcg                                                                                                     >  2:2610378/1‑137 (MQ=255)
                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCACAATATTTCGCACATGGTTTTTCACCGGTTTCTCACTGCTAAACAATGATTCAACAATAcc                                                                                                     >  1:677459/1‑140 (MQ=255)
                                    taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAAAATTTCTCACAATGTTTTTCACGGTTTTATCACTGATAAACAAATATTAACCaaaaac                                                                                                     >  1:911156/1‑136 (MQ=255)
                                      cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTAAATGTTAAAAAATTATTCAACAATACcgc                                                                                                   >  2:217139/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATTGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATAACGCgg                                                                                                 >  2:40719/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTAACCAATACCGCgg                                                                                                 >  2:3710348/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTCTCACATGGTTTTTAAAGGTTTTATAAATGATAAAAAATTATTAAACAATACCGCgg                                                                                                 >  2:3266466/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAATATTTCTCACATTGTTTTTCACGGTTTTCTCACTGAGAAACAAATATTAACCAATAACCCgg                                                                                                 >  2:3777610/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAAAAATGCGCACAAGGTTTTTCACCGTTTTGTCACAGATAAAAAAATATTAACCCATAACCCgg                                                                                                 >  1:878191/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCgg                                                                                                 >  2:3809894/1‑140 (MQ=255)
                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGAGTAAAATCATTTTTTGCAAAATAATGCACACATGGTTTTTCACGCTTTTATCACTGATAAAAAAATAATCACCAATACCGCgg                                                                                                 >  2:901050/1‑140 (MQ=255)
                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAAAGATTAACCAAAAACCCGGt                                                                                                >  1:3252030/1‑140 (MQ=255)
                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCggg                                                                                                <  1:3033862/140‑2 (MQ=255)
                                         tctcAGTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTGCATTAATTTTTTGCAAAATAATGCTCCCATGGGTTTTAACGGTTTTCTCACTGAAAAAAAATGATTCACCAATACCTCGGt                                                                                                >  2:1955105/1‑140 (MQ=255)
                                          ctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTTTCACTGATAAAAAATGATTAACCAATACCGCGGtt                                                                                               >  2:1066645/1‑140 (MQ=255)
                                              tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTTCTGACATGGGTTTTCACGGTTTTAACACTGATAAACAATGATTCACCAAAACCGCCGGTGCtt                                                                                           >  2:3674906/1‑140 (MQ=255)
                                                tCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCtttt                                                                                         <  2:1778810/140‑1 (MQ=255)
                                                   aCCCAGCCATTTTTAATGGCGACCACAACTGCTTGCGTCCGGTCGTTTACATTAATTTTTTGAAAAATATTGCTAACATGGTTTTTAACGGTTTTCTCACAGATAAAAAAAGATTCACCAAAACCGCGGTTGCtttttac                                                                                      >  2:1475899/1‑138 (MQ=255)
                                                   aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                                                      <  1:3808891/140‑1 (MQ=255)
                                                   aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                                                      >  2:821890/1‑140 (MQ=255)
                                                   aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTTCAAAATATTGGGCCCATGGTTTTTCACGGTTTTCTGACTGATAAACAATGATTAACCAATACCGCGGGTGGTTTTTcc                                                                                      >  1:2678821/1‑140 (MQ=255)
                                                          cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCa                                                                               <  1:1587477/140‑1 (MQ=255)
                                                             ttttAATGGCGACCCCAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                            <  2:3963705/140‑1 (MQ=255)
                                                                  aTGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAATCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                                                       <  1:3003723/140‑1 (MQ=255)
                                                                   tGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAAAATTGCTCACATGGTTTTTGACCGGTTTGTGACTGATAAAAAAAGTTTCACCAATACCGCGGGTGGTTTTTACGTCTGGAAGCAGCTgc                                                                      >  1:2488154/1‑140 (MQ=255)
                                                                     gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGTTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                    <  1:2627878/140‑1 (MQ=255)
                                                                     gCGACCACAACGGCTGGCGTCCGGTCGTTTACATTCATTTTTTGCCAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                    <  2:30228/140‑1 (MQ=255)
                                                                       gACCACACCGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTgcagca                                                                  <  1:2306255/140‑1 (MQ=255)
                                                                       gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                                  <  1:2664020/140‑1 (MQ=255)
                                                                            caACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                                             <  1:3686898/140‑1 (MQ=255)
                                                                              aCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTcaca                                                           <  2:1018499/140‑1 (MQ=255)
                                                                                    tGCGTCCGGTCGTTTACATTCATTTTTTTGAAAATATTGGTCACATGGTTTTTCACGGTTTTGTGACTGATAAAAAATTATTAAACAATACCGCGGTTGGTTTTTGCGTGTGCAAGACTCTGGAGCACTTCCCAGTAccg                                                     >  2:3504521/1‑140 (MQ=255)
                                                                                    tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCccg                                                     <  2:958496/140‑1 (MQ=255)
                                                                                          cGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGt                                               <  2:3196374/140‑1 (MQ=255)
                                                                                           ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                              <  1:2837512/140‑1 (MQ=255)
                                                                                           ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                              <  1:375883/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATAGCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                           <  1:1475899/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                           <  1:3703940/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                           <  2:1409341/140‑1 (MQ=255)
                                                                                              cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATGCCCGCCGAGTTaaa                                           <  2:1894954/140‑1 (MQ=255)
                                                                                              cGTTTACATTCAATTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                           <  1:514774/140‑1 (MQ=255)
                                                                                                   aCATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATg                                      <  2:558916/140‑1 (MQ=255)
                                                                                                         attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                                >  2:1579687/1‑140 (MQ=255)
                                                                                                          ttttttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGt                               <  2:1214012/140‑1 (MQ=255)
                                                                                                             tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg                            <  2:2877715/140‑1 (MQ=255)
                                                                                                               tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCACTACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                          <  1:1244036/140‑1 (MQ=255)
                                                                                                               tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                          <  1:431150/140‑1 (MQ=255)
                                                                                                               tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                          <  2:1622993/140‑1 (MQ=255)
                                                                                                                    aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                     <  1:2572341/140‑1 (MQ=255)
                                                                                                                    aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                     <  1:3090979/140‑1 (MQ=255)
                                                                                                                         tGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGa                >  2:2415339/1‑140 (MQ=255)
                                                                                                                         tGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGa                <  2:1254746/140‑1 (MQ=255)
                                                                                                                          gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt               <  1:3149112/140‑1 (MQ=255)
                                                                                                                          gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt               <  1:853662/140‑1 (MQ=255)
                                                                                                                          gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt               <  2:1132025/140‑1 (MQ=255)
                                                                                                                            tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg             <  1:3898719/140‑1 (MQ=255)
                                                                                                                              acaTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc           <  2:3536629/140‑1 (MQ=255)
                                                                                                                              acaGGGTGTGTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCGGCAAGCATCTGCAGCACTTCCCATTCCCGCCGAGTTAAAAGATGTAATGGTCTGCGGATTTCAGGATAGAc           <  2:3110736/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                           <  1:131452/106‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         >  1:151857/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:2066865/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  2:3843499/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         >  1:2790332/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:3504521/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         >  2:3448460/1‑140 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:3365281/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:3467050/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:961202/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:3523516/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:3713979/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  2:2275636/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:622714/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:2129997/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  2:2921054/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  1:1089412/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTCACGGTTTTCTCACCGATAAAAAATGATTCACCAATAACGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCATCACTTCACCTTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt         <  2:679062/140‑1 (MQ=255)
                                                                                                                                aTGGTTTTTAACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAtt                                                         >  2:2843992/1‑92 (MQ=255)
                                                                                                                                       ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg  <  1:3195297/140‑1 (MQ=255)
                                                                                                                                       ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg  <  1:1018546/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTG  >  NZ_CP034943/3446858‑3447132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: