Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,447,038 T→G 12.8% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,447,0380TG12.8% 439.4 / 12.5 259N183T (AAC→ACC) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base T (76/137);  new base G (8/38);  total (84/175)
Fisher's exact test for biased strand distribution p-value = 1.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176
                                                                                                                                           |                                                                                                                                          
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTAAACAAAACCGCGGt                                                                                                                                            >  2:786139/1‑140 (MQ=255)
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAAAGATTAACCAAAAACCCGGt                                                                                                                                            >  1:3252030/1‑140 (MQ=255)
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTTCTCAAATGGTTTTTCAAGGTTTTATAACTGATAAAAAATAATTCACCAAAAACGCGGt                                                                                                                                            >  2:1441492/1‑140 (MQ=255)
tctcAGTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTGCATTAATTTTTTGCAAAATAATGCTCCCATGGGTTTTAACGGTTTTCTCACTGAAAAAAAATGATTCACCAATACCTCGGt                                                                                                                                            >  2:1955105/1‑140 (MQ=255)
     tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTTCTGACATGGGTTTTCACGGTTTTAACACTGATAAACAATGATTCACCAAAACCGCCGGTGCtt                                                                                                                                       >  2:3674906/1‑140 (MQ=255)
     tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAAAGATTCACCCATAACGCGGTTGCtt                                                                                                                                       >  2:1244036/1‑140 (MQ=255)
     tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAATATTTCTTACATGGTTTTTCAACGTTTTATCACTGATAAACAAAGATTCAACAAAAACCCGGTTGCtt                                                                                                                                       >  1:837773/1‑140 (MQ=255)
      ttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCAATTTTTGCAAAATATTGCTAACATGGTGTTTAACCGTATTCTCACTGAAAAACAATGATTCACCAATACCCCGGTTGCttt                                                                                                                                      >  2:3190572/1‑140 (MQ=255)
          aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATATTTTTCAAAATATTGCTCCCAGTGTTTTTAACGGTTTTATAACTTATAAAAAATTATTACACAATAACGCGGGTTCtgtttac                                                                                                                                  >  2:580001/1‑138 (MQ=255)
            ccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGt                                                                                                                                <  1:875474/140‑1 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                                                                           <  1:2482271/140‑1 (MQ=255)
                    ttttAATGGCGACCCCAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  2:3963705/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  2:1618502/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTATTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  2:1281213/140‑1 (MQ=255)
                         aTGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAATCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTg                                                                                                                   <  1:3003723/140‑1 (MQ=255)
                          tGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAAATAATTTTTTTGAAAAAATTTGTAACAAGGTTTTTAAAGGGTTTATAACAGATAAAAAATGGTTAACCAAAAAACCGGTTTCTTTTTTCGTCTGCAAgaaaatga                                                                                                                  >  1:1309791/1‑133 (MQ=255)
                            gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  1:266550/140‑1 (MQ=255)
                            gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  1:2076227/140‑1 (MQ=255)
                               aCCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGGTTTCTCACTGATAAACAATGATTAAACAATACCCCGGTTGCTTTTTCCGGCTGCAAGCAGCTGCAGCAc                                                                                                             >  2:1081051/1‑140 (MQ=255)
                                     aCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTcaca                                                                                                       <  2:1018499/140‑1 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCCCAAATGGTTTTTAACGGTTTTGTCACAGATAAAAAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAACCATCTGCAGCACATAACATTCccg                                                                                                 >  2:3810566/1‑140 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATTGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                                                                                 <  2:1494098/140‑1 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                                                                                 <  1:16018/140‑1 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                                                                                 <  1:151925/140‑1 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATAACGCGGTTGCTTTTTCCGTCTGCAAGCATCAGCAGCACTTCACATTCccg                                                                                                 >  2:1872565/1‑140 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGAAAAATATTGCTAACATGGTTTTTAACGGTTTTGTCACTGATAAAAAATTATTCACCAATACCGCGGTTGCTTTTTACGTCTGCAACCATCGGCAGCCCTTCACATTCccg                                                                                                 >  2:3606157/1‑140 (MQ=255)
                                             cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                                                                               <  2:2083605/140‑1 (MQ=255)
                                                 cGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGt                                                                                           <  2:3908894/140‑1 (MQ=255)
                                                  ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                                                                          <  1:2248749/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATAGCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:1475899/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:1407759/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:3055808/140‑1 (MQ=255)
                                                     cGTTTACATTCAATTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:514774/140‑1 (MQ=255)
                                                     cGTTTACATTAATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTTATAAACAATGATTCACCAATAGCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:2836861/140‑1 (MQ=255)
                                                     cGTTGACATGCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:2279411/140‑1 (MQ=255)
                                                     cGTTGACAGTCATTTTTTGCAAAATATTGCTCACATGGTTTGTCACGGGTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:2226807/140‑1 (MQ=255)
                                                     attttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCTCTGTTTTCTCCCTGATAAACAATGATTCACCTATAGCGAGGTTGCTTTTTCCGTCTGCAAGCATCTGCATCACTTCAAATTCCCGCCGAGTTaaa                                                                                       <  1:2639746/138‑1 (MQ=255)
                                                          aCATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATg                                                                                  <  2:3521385/140‑1 (MQ=255)
                                                            attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATATGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                                                                <  1:284514/140‑1 (MQ=255)
                                                                attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAATATATGTAATgg                                                                            >  1:2512263/1‑140 (MQ=255)
                                                                attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                                                                            >  2:850874/1‑140 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:431150/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:3121819/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  2:3051224/140‑1 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  1:671083/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:2942227/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:3638791/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  2:1132025/140‑1 (MQ=255)
                                                                                   tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTCATGGTCTGCGGATTTCAGGATAg                                                         <  2:949276/140‑1 (MQ=255)
                                                                                   tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg                                                         <  1:3809894/140‑1 (MQ=255)
                                                                                   tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg                                                         <  1:3266466/140‑1 (MQ=255)
                                                                                     acaTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc                                                       <  1:967899/140‑1 (MQ=255)
                                                                                     acaTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc                                                       <  1:3710348/140‑1 (MQ=255)
                                                                                     acaTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc                                                       <  2:2222646/140‑1 (MQ=255)
                                                                                     acaGGGTGTGTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCGGCAAGCATCTGCAGCACTTCCCATTCCCGCCGAGTTAAAAGATGTAATGGTCTGCGGATTTCAGGATAGAc                                                       <  2:3110736/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTTCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:3767607/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  2:3448460/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:3467050/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:3504521/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:3365281/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:2887198/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:3694201/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:2790332/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:2415339/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:622714/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1916356/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:418938/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1089412/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1341610/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAACAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1187820/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACCGATAAAAAATGATTCACCAATAACGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCATCACTTCACCTTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:679062/140‑1 (MQ=255)
                                                                                       aTGGTTTTTAACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAtt                                                                                                     >  2:2843992/1‑92 (MQ=255)
                                                                                              ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg                                              <  1:1018546/140‑1 (MQ=255)
                                                                                              ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg                                              <  1:3195297/140‑1 (MQ=255)
                                                                                                 aCGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTCAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAgg                                           <  2:1107679/140‑1 (MQ=255)
                                                                                                 aCGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAgg                                           <  2:2575055/140‑1 (MQ=255)
                                                                                                  cGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  2:869126/140‑1 (MQ=255)
                                                                                                  cGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  1:4023097/140‑1 (MQ=255)
                                                                                                  cGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  2:1703923/140‑1 (MQ=255)
                                                                                                  cGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  1:1718055/140‑1 (MQ=255)
                                                                                                    gTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGgtg                                        <  2:3295673/140‑1 (MQ=255)
                                                                                                     ttttCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                                                                                 <  2:2956975/82‑1 (MQ=255)
                                                                                                        tctcACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCa                                    <  1:786139/140‑1 (MQ=255)
                                                                                                              tGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACt                              <  1:923714/140‑1 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  2:2351651/1‑140 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  1:2602333/1‑140 (MQ=255)
                                                                                                                          aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  2:2913665/1‑105 (MQ=255)
                                                                                                                          aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  1:2957251/1‑140 (MQ=255)
                                                                                                                            tCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAg                <  2:2663305/140‑1 (MQ=255)
                                                                                                                               ccAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggag             >  2:3467783/1‑138 (MQ=255)
                                                                                                                                  aTACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGgcggcg          >  1:1137217/1‑140 (MQ=255)
                                                                                                                                      cgcgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAAt      >  1:3535199/1‑140 (MQ=255)
                                                                                                                                      cgcgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAAt      <  2:2665667/140‑1 (MQ=255)
                                                                                                                                      cgcgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAAt      <  2:1959351/140‑1 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  2:1538758/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAAGATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGACTTc    >  2:704424/1‑140 (MQ=255)
                                                                                                                                        cgGTTCCTTTTTCCGTCTGCAAGCAACTGCAGAACTTCACAGTCCCGCCGAGTTAAATTATGTAATTGACTGCCCATTTCAGCATATAACTCATCCTGAGCGTGTGAACAAAAACCGCATCTAGCAAGAAGACGGAAtaa    >  2:2405670/1‑138 (MQ=255)
                                                                                                                                         ggTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                                                                     <  1:3853946/58‑1 (MQ=255)
                                                                                                                                         ggTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                                                                     >  2:3853946/1‑58 (MQ=255)
                                                                                                                                          gTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGt  >  1:2137799/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: