Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,539,589 T→G 7.5% I122L (ATC→CTC)  EO946_RS18215 ← T7SS effector LXG polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,539,5890TG 7.5% 566.2 / 4.1 223I122L (ATC→CTC) EO946_RS18215T7SS effector LXG polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base T (91/106);  new base G (23/3);  total (114/109)
Fisher's exact test for biased strand distribution p-value = 3.73e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

AGGGATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAAC  >  NZ_CP034943/3539450‑3539725
                                                                                                                                           |                                                                                                                                          
aGGGATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAt                                                                                                                                            >  2:3918902/1‑140 (MQ=255)
aGGGATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAt                                                                                                                                            <  2:3718049/140‑1 (MQ=255)
aGGGATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAt                                                                                                                                            <  2:2101884/140‑1 (MQ=255)
   gATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttc                                                                                                                                         >  2:223490/1‑140 (MQ=255)
   gATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttc                                                                                                                                         >  2:3402078/1‑140 (MQ=255)
     ttCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttctt                                                                                                                                       >  2:3892100/1‑140 (MQ=255)
     ttCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttctt                                                                                                                                       >  2:385757/1‑140 (MQ=255)
      tcatcaAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATttcttc                                                                                                                                      >  1:3945783/1‑140 (MQ=255)
       catcaAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTtgttct                                                                                                                                    >  2:2552440/1‑140 (MQ=255)
            aGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTAtt                                                                                                                               >  1:648314/1‑139 (MQ=255)
            aGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTCTTGTTTATg                                                                                                                               >  1:2972109/1‑140 (MQ=255)
             gCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTAtta                                                                                                                              >  2:2816708/1‑138 (MQ=255)
              ataagagACAGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAg                                                                                                                             >  1:1866178/7‑140 (MQ=255)
              ataagagACAGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCGTCTTTATGAg                                                                                                                             >  1:1759291/7‑140 (MQ=255)
                 aTCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGATCgg                                                                                                                          >  1:2630926/1‑140 (MQ=255)
                  tCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGGTTATTAGCGga                                                                                                                         >  2:1089114/1‑140 (MQ=255)
                     aCTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGAc                                                                                                                      <  1:1489797/140‑1 (MQ=255)
                      gtgctgTGAGTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTGTATGAGCGGAGACa                                                                                                                     <  1:47083/139‑1 (MQ=255)
                       tgctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCGTGTTTATGAGCGGAGACaa                                                                                                                    >  2:1497068/1‑140 (MQ=255)
                       tgctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTGTTTTTGAGCGGAGACaa                                                                                                                    >  2:3176854/1‑140 (MQ=255)
                       tgctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTCTTCTTTTTGAGCGGCGAcca                                                                                                                    >  2:3628007/1‑138 (MQ=255)
                        gctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTCTTTATGAGCGGAGACAAt                                                                                                                   >  2:1212710/1‑140 (MQ=255)
                         ctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGGGCGGGGACacat                                                                                                                  >  1:2259286/1‑137 (MQ=255)
                         ctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGATCGGAGACCAtt                                                                                                                  >  1:2558508/1‑140 (MQ=255)
                         ctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGAc                                                                                                                      >  2:1490420/1‑136 (MQ=255)
                         ctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACaaat                                                                                                                  >  2:3684283/1‑138 (MQ=255)
                         ctgTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATGTGTTGTTTTTGAGCGGAGACCAtt                                                                                                                  >  1:3071044/1‑140 (MQ=255)
                             gATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTg                                                                                                              <  2:1545326/140‑1 (MQ=255)
                               ttGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAg                                                                                                            <  1:181187/140‑1 (MQ=255)
                                 gtgtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGAGCGGAGACAAATTATGATGc                                                                                                          >  1:339490/1‑140 (MQ=255)
                                 gtgtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTGTTTATGAGCGGAGACAAATTCTGAGGc                                                                                                          >  1:3169181/1‑140 (MQ=255)
                                 gtgtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTCTTTATGAGCGGAGTCAATTTCTGAGGc                                                                                                          >  1:2798077/1‑140 (MQ=255)
                                 gtgtCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGGTGGTCTTTTTGTTCGGAGAAAATTTCTGAGGc                                                                                                          >  1:2986065/1‑140 (MQ=255)
                                    tCACGTCTGATTTTTTGGGCATCGCCCATCTTATCTGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATAGAATCGATTTCTTCTTTATGAGCGGAGACAATCTCTGAGGCttt                                                                                                       >  2:611367/1‑140 (MQ=255)
                                    tCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCGGAGTCCATTTGTGATGCttt                                                                                                       >  2:3328686/1‑140 (MQ=255)
                                    tCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCttt                                                                                                       <  2:525180/140‑1 (MQ=255)
                                    tCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCttt                                                                                                       <  2:2122114/140‑1 (MQ=255)
                                    tCACGTCTGATATATTCGGAATCTCCCATCTTATCGGCATAGTCGTGAAGCTTGTAACTGTCGAGATCAATAATATAGCTTATGCCTGACAAAATGGAGTCGATTTCTTCATGTATAGAGGAGACAATGTGAGAGGCtgg                                                                                                       >  2:1822596/1‑138 (MQ=255)
                                     cACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTCCATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCAGAGGCTTTc                                                                                                      <  1:883374/140‑1 (MQ=255)
                                       cGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCaa                                                                                                    <  2:2844853/140‑1 (MQ=255)
                                         tCTGATTTTTTGGGCATCTCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                  <  2:642887/140‑1 (MQ=255)
                                         tCTGATTTTTTGGGCATCGCCCATCTTATCTGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATAGAATCGATTTCTTCTTTATGAGCGGAGACAATCTCTGAGGCTTTCAGGt                                                                                                  <  1:1130411/140‑1 (MQ=255)
                                         tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATGAGCCGAGACAAATTCTGAGGCTTTCAAGt                                                                                                  >  1:967606/1‑140 (MQ=255)
                                         tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                  <  1:3628007/140‑1 (MQ=255)
                                         tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                  <  2:3035863/140‑1 (MQ=255)
                                         tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                  <  1:128000/140‑1 (MQ=255)
                                         tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGt                                                                                                  <  2:536250/140‑1 (MQ=255)
                                         tCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTGCAAGt                                                                                                  <  1:1295440/140‑1 (MQ=255)
                                            gATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGATCGGAGACAATTTCTGGGGCTTTGAAGtctc                                                                                               >  2:3765895/1‑140 (MQ=255)
                                              ttttttGGGCATCGCCCATCTTATCTGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATAGAATCGATTTCTTCTTTATGAGCGGAGACAATCTCTGAGGCTTTCAGGTCTCCa                                                                                             <  1:611367/140‑1 (MQ=255)
                                              ttttttGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCa                                                                                             <  2:2978427/140‑1 (MQ=255)
                                              ttttttGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCa                                                                                             <  1:2093956/140‑1 (MQ=255)
                                              ttttttGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCa                                                                                             <  1:23305/140‑1 (MQ=255)
                                                   tGGGCAGCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGAACAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTggg                                                                                        <  2:164335/140‑1 (MQ=255)
                                                                  ttATCGGCATAGTCGTCAAGCGTGTACATGTCGAGAGCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTATTTTTGAGCGGATACAATTTCTGAGGCTTTCAATACTCCATTGGGGCGCTCATGATccca                                                                         >  2:2024137/1‑138 (MQ=255)
                                                                    aTCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTATTCTTTATGAGCGGAGAAAATTTCTGAGGCTTTCAAGTCTCCATTTGGCAGCGAATGATCCaaaa                                                                       >  2:3737233/1‑140 (MQ=255)
                                                                         cATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaagaa                                                                  <  2:1693094/140‑1 (MQ=255)
                                                                         cATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaagaa                                                                  <  2:1759291/140‑1 (MQ=255)
                                                                         cATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAaagaa                                                                  <  2:1866178/140‑1 (MQ=255)
                                                                           tAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATTAGCGGAGACAAATTGTGAGGCTTTCAAGTCTCCCATTGGCACCGCATGATACAAAAAATAAGt                                                                >  1:3425152/1‑140 (MQ=255)
                                                                              tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                             <  2:10658/140‑1 (MQ=255)
                                                                              tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                             <  2:3945783/140‑1 (MQ=255)
                                                                              tcgtcAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGACACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTc                                                             <  2:551685/140‑1 (MQ=255)
                                                                                   gaGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACat                                                       <  1:1554825/139‑1 (MQ=255)
                                                                                        tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGAGCGGATACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATACAAAAAATAAGTTTACACATATGaa                                                  >  2:3445511/1‑140 (MQ=255)
                                                                                        tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGaa                                                  <  1:4010926/140‑1 (MQ=255)
                                                                                        tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTATGAGCGGAGACAAATTCTGTGGCTTTCAAGTCTCCCTTTGGCAGCGCAAGATACAAAAAAGAAGTTTGCACataataa                                                  >  1:2136610/1‑136 (MQ=255)
                                                                                        tgtACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGGTGGTGGTTTTGAGCGGCGACAAATTCTGAGGCTTTGAAGTGTGCCATGGGCATCGCAATATACCCAAAACAACTTTGCACAAATgta                                                  >  1:3595356/1‑138 (MQ=255)
                                                                                        tggccATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCGTTCACGTCTCCATTGGGCAGCTCATGATCCAACAAAGAAGGTTCCACATCTGaa                                                  <  2:684386/136‑1 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGCGACAAATTCTGAGGCTTTCAAGGCGCCATTGGGCAGCTCATGATCCAAAAAAGAAATTTACACAAATGGATc                                                >  2:3726275/1‑140 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTAAAGTCTCCATTTGGCAGCTCATGATCCAAAAAAGAAGTTTACACATATTAAta                                                >  2:2167854/1‑139 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTCGTTTTGAGCGGAGACAATTTGTGTGGCTTTGAAGTCTGCAGTGTGGCGCTCATGATGCAAAAAAGTAGTTTACACaaattgatc                                                >  1:3576835/1‑135 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGAc                                                                                                                      <  1:337097/71‑1 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTGTCCATTGGGCAGCAAATGATCCAAAAAATAAGTTTGCACATATTAATc                                                >  2:1978392/1‑140 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTATTTATTAGCCGAGACAAATTGTGAGGATTTAAAATCTACATTGGGCAGCTCATGATACAAAAAAGAAGTTTCCAAATATTAAta                                                >  1:1010692/1‑139 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTATTTATGAGCGGAGACAAGTTCTGAGGCTTTCAAGTCGCCATTGGGCAGCTCATGATCCAAAAAAGTAGTTTACACCTATGaaac                                                >  2:793090/1‑138 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGTTGTTGTTTTTTAGCGGAGACAAATTCTTAGGCTTTGAAGTCTCCCATTGGCAGCTCAATATCCAAAAAAAAAATTTACACAAATGaaaa                                                >  1:1033682/1‑138 (MQ=255)
                                                                                          tACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGAGGTGTTCTTTATGAGCGGAGACAAATTCTGAGGCTTTCAATTCTCCATTGTGCAGCTCATGATCAAAAAAAGAATTTTCCACATATTaaaa                                                >  2:1354016/1‑138 (MQ=255)
                                                                                             aTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCAtt                                             <  2:3550141/140‑1 (MQ=255)
                                                                                             aTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCAtt                                             <  2:1231148/140‑1 (MQ=255)
                                                                                             aTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCAtt                                             <  2:1606247/140‑1 (MQ=255)
                                                                                             aTGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTAGGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAACAGAAGTTTCCACATATGAATCAtt                                             <  1:3756674/140‑1 (MQ=255)
                                                                                              tGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTc                                            <  1:1452472/140‑1 (MQ=255)
                                                                                              tGTAGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTc                                            <  1:2959602/140‑1 (MQ=255)
                                                                                              gggcGAGAGCAATAAGATCGCTGATGCCTGACAAAATGGAGTAGATTTCTTCTTTACGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTc                                            <  1:124613/137‑1 (MQ=255)
                                                                                              agacaggaTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTc                                            <  2:3155544/134‑1 (MQ=255)
                                                                                                  gagaTCGATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTGTTTATTATCGGAGACAATTTCTGTGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAATAAGTTTCCACATATTAATAATTAAGtt                                        >  2:241928/1‑140 (MQ=255)
                                                                                                     aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                     <  1:1964178/140‑1 (MQ=255)
                                                                                                     aTCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCtt                                     <  1:3783008/140‑1 (MQ=255)
                                                                                                      tCAATAATATCGTTGATGCCTGACAAATTGGAGTAGATTTCTTCTTTATGAGCAGATACAATTTCTGAGGCTTTCAAGTCTACATTGGTCATCTCATGATGCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                    <  2:2317912/140‑1 (MQ=255)
                                                                                                      tCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTg                                    <  1:1494308/140‑1 (MQ=255)
                                                                                                       cAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTGTTGTTTATGAGCGGAGACAAATTCTGAGGCTTTGAAGTCTCCATTGGGCCGCTCATGATACAAAAAATAAGTTTACACAAATTAATAATTCAGttgttgt                                   >  1:3110918/1‑139 (MQ=255)
                                                                                                               tCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTGAAGTCTCCATTGGGCAGCTCATGATCCCAAAAAGAAGTTTACACATATTAATAATTCAGTTCTTGATATTTTAt                           >  2:1745768/1‑140 (MQ=255)
                                                                                                                 gCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAAACATTCAGTTCTTGATCTTTAATGt                         <  2:883222/140‑1 (MQ=255)
                                                                                                                  cTGATGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAAATTCTGAGGCTTTGAACTCTACATTGGGCAGCTCATGATCCAAAAAAGAAGTTTACACATATGAATCATTAAGGTCTTGTTATTTAATTtc                        >  2:3829214/1‑140 (MQ=255)
                                                                                                                  cTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTATGCGGCGTTAAAGTCTCCAGTGGGCAGCTCCTGATCCAAAAAAAAAGTTTCCACAAATGAAACAATCAGTTCTTGATATTTAATGtc                        >  2:1282147/1‑140 (MQ=255)
                                                                                                                  cTGATGCCTGACAAAATGGAGTCGAGTTGTTCTTTATGAGCGGCGACAAATTCTGAGGGTTTAAAGTCTCCCATTGGCAGCGCATTATCCAAAAAATAAATTTACACAAAAGAAACATTCAATTGTTGCTCTTTAATGtg                        >  1:3861201/1‑139 (MQ=255)
                                                                                                                     aTGCCTGACAAAATGGAGTCGATTTGTTCTTTATGAGCGGAGACAAATTCTGAGGCTTTCAAGTGTCCAATGGGCAGCGCATGATACAAAAAAGAAGTTTCCACAAAATAATCATTCAGTTGTTGATATTTAATTTATcc                     >  2:1384983/1‑140 (MQ=255)
                                                                                                                     aTGCCTGACAAAATGGAGTCGATTTATTCTTTATGAGCGTCGACCAATTGATATGCTTTCAACTCACCATTGGGCAGCTCATTTAACAAAAAATAACTTTCCACAATATAATGAGTGTGTTGTTGTTCTTTAATTtctgc                     >  2:2940472/1‑138 (MQ=255)
                                                                                                                         cTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGaa                 <  2:3231923/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGGGATTCATCAAGCTTATCGACTGCTGTGATTGTGTCACGTCTGATTTTTTGGGCATCGCCCATCTTATCGGCATAGTCGTCAAGCGTGTACATGTCGAGATCAATAATATCGCTGATGCCTGACAAAATGGAGTCGATTTCTTCTTTATGAGCGGAGACAATTTCTGAGGCTTTCAAGTCTCCATTGGGCAGCTCATGATCCAAAAAAGAAGTTTCCACATATGAATCATTCAGTTCTTGATCTTTAATGTCTCCTGAAATGCCATTTAAAAAC  >  NZ_CP034943/3539450‑3539725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: