BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,359	A→C	13.9%	T68P (ACC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,359	0	A	C	13.9%	313.8 / 15.7	169	T68P (ACC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (52/87); new base C (4/25); total (57/112)
Fisher's exact test for biased strand distribution p-value = 1.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

AGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607220‑607498

aGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCaa                                                                                                                                             <  1:1569114/140‑1 (MQ=255)

aGCCACGGGAGCTACTGGGGAAGCGGGACCTGCCGCACCAGCGGGCGCGAGGGGAGCGATGGGAGCTACCGGGCAAGATGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCaa                                                                                                                                             <  1:3538623/140‑1 (MQ=17)

 gCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                                                                            <  2:2206326/140‑1 (MQ=255)

    aCGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCAACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGAGACGGGAGCGACAGGAGCTACAGGCGAAGCCGGACCATCCGGACCAACCgg                                                                                                                                         >  2:2981066/1‑140 (MQ=18)

         aGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGa                                                                                                                                    >  2:2846988/1‑140 (MQ=255)

           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:1115156/140‑1 (MQ=255)

           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGCACAGACAGGCGCAGCCCGAGACGCCGCACCGGCCCGAGCCACCGGAGCGACg                                                                                                                                  >  1:2963273/1‑140 (MQ=17)

             aCTGGGGAAGCTGGACCAGCCGGACCTGCCGGAGCGACGGGAACCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  2:1100283/140‑1 (MQ=255)

             aCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACggg                                                                                                                                <  1:2450549/140‑1 (MQ=255)

              cTGGTGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACTGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGa                                                                                                                               <  2:1944691/140‑1 (MQ=255)

                 gggAAGCAGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGcc                                                                                                                            <  2:282012/140‑1 (MQ=255)

                   gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGAc                                                                                                                          >  1:3595948/1‑140 (MQ=255)

                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGTTGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:188816/140‑1 (MQ=255)

                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGATGCCGCACAAACACGACCGACGGGAGCGACGTGGGACACggg                                                                                                                       >  2:1034162/1‑140 (MQ=18)

                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:2583404/140‑1 (MQ=255)

                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:3557967/140‑1 (MQ=255)

                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       >  2:1901157/1‑140 (MQ=255)

                           accagccggaccagccggAGCGACGGGAGCCACCGGAGCGACCGGGGCAGCTGGACCCGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  2:146025/140‑1 (MQ=255)

                               gccggaccatccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACCGCAGCGACAGGCGCAACCCGGGACGCCGGACCAGCCGGACCAACCGGAGCCACGGGAGCGACGCGAGCTACCgt                                                                                                              >  2:808839/1‑139 (MQ=18)

                                ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  1:1032265/140‑1 (MQ=255)

                                ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                             <  1:1113472/140‑1 (MQ=255)

                                    accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAag                                                                                                         <  1:1353536/140‑1 (MQ=255)

                                    accagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGCGCTACCGGGGAag                                                                                                         >  1:368189/1‑140 (MQ=255)

                                        gccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgg                                                                                                     <  2:214909/140‑1 (MQ=255)

                                          cggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAagccggac                                                                                                   >  2:3671968/1‑140 (MQ=255)

                                          cggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAagccggac                                                                                                   >  2:3712637/1‑140 (MQ=255)

                                          cggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggac                                                                                                   >  2:2687100/1‑140 (MQ=255)

                                          cggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGTGCACCCGGTGCAGCCGGACCACCCGGAGCGACGGGAGCGACGGGAGCGACCGGCGCagccgggc                                                                                                   >  2:2893774/1‑138 (MQ=11)

                                             aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGCGCGACGGGAGCCAGGGGAGCTACCGGGGAagccggaccag                                                                                                >  1:811522/1‑140 (MQ=255)

                                             aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  1:1403844/140‑1 (MQ=255)

                                             aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  1:3717898/140‑1 (MQ=255)

                                             aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  1:1338352/140‑1 (MQ=255)

                                              gagACGGGAGCATCGTGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagc                                                                                               <  2:3735880/138‑1 (MQ=17)

                                              gCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagc                                                                                               <  2:2224919/140‑1 (MQ=255)

                                              gCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagc                                                                                               <  2:3310473/140‑1 (MQ=255)

                                               cGACGGGCGCCACGGGAGCGACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagcc                                                                                              <  1:2735365/140‑1 (MQ=255)

                                                   gggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccggac                                                                                          <  1:3796210/140‑1 (MQ=255)

                                                   gggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCAACCGGAGCGACCGGAGACACGGGAGCAACCGGGGAcgcaggaccagccggac                                                                                          >  1:516055/1‑140 (MQ=17)

                                                   ggccgCCACGGCAGCTACCGGGGACGCGGGACCAGCCGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCCGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccggac                                                                                          <  1:2120447/136‑1 (MQ=17)

                                                     gAGCCACGGGCGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAgccggaccagccggacca                                                                                        <  2:3088002/140‑1 (MQ=255)

                                                     gAGCCACGGGAGCGACCGGGGCAGCTGGACCAGCCGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAgccggaccagccggacca                                                                                        <  2:1806705/140‑1 (MQ=255)

                                                      aGCCACCGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCGACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCaa                                                                                       <  2:2523239/140‑1 (MQ=14)

                                                       gCCACGGGAGCTACCGGGGACGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                      <  1:657954/140‑1 (MQ=255)

                                                       gCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                      <  1:232117/140‑1 (MQ=255)

                                                       gCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                      <  1:3224639/140‑1 (MQ=255)

                                                        ccACGGGAGCTACCGGGGAAGCTGGACCAGCCGGCGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                     <  2:2991663/140‑1 (MQ=255)

                                                          ccGGGCGCTCCGGGGGAAGCTGGACCACGCGGTGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                   <  2:1519013/139‑1 (MQ=18)

                                                               aGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcga                                                                              <  1:3917903/140‑1 (MQ=255)

                                                                   aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:95433/140‑1 (MQ=255)

                                                                   aCCGGGGAAGCCGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGCCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:2028797/140‑1 (MQ=255)

                                                                         gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:932286/140‑1 (MQ=255)

                                                                         gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:1304911/140‑1 (MQ=255)

                                                                         gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:1930928/140‑1 (MQ=255)

                                                                         gAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  1:3823563/140‑1 (MQ=255)

                                                                         gAAGCTGGACCAGCAGGAGCCACGGGAGCCACAGGAGCTACCGGTGAAGCAGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:3799705/140‑1 (MQ=17)

                                                                           aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                  >  1:871388/1‑140 (MQ=255)

                                                                           aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                  >  1:1714099/1‑140 (MQ=255)

                                                                            gacggACCAGCGGTAGCGCGGGGAGAGAATGGAGCTACCGGGGAAGCCGGACCAGCCGGACAAACCGTCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGAACAGCCGGACCAACCGGagcgacaggagcgacagg                                                                 <  1:1469337/137‑1 (MQ=17)

                                                                            gCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                 <  1:1741124/140‑1 (MQ=255)

                                                                              tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgaccggagcgacaggag                                                               >  1:387894/1‑140 (MQ=255)

                                                                               ggACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCgacaggagcgacaggagc                                                              >  1:710359/1‑140 (MQ=255)

                                                                                     gCCGGAGCGCCGGGCGCGACCGGAGCCACCGGGGAAGCCGGACCAGCCGGACCACCCGGACCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                        <  2:2658895/140‑1 (MQ=17)

                                                                                     gCCGGAGCGACGGGAGCGCCAGGAGCTACCGGGGACGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                        <  2:3855135/140‑1 (MQ=255)

                                                                                     gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCACCCGGAGCGACCGGAGCGACAGGAGCTACCgg                                                        >  2:220758/1‑140 (MQ=14)

                                                                                     gCCGGAGCGACGGGAGCCACAGGAGCTCCCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                        <  2:1277320/140‑1 (MQ=255)

                                                                                     gCCGGAGCGACGGGAGCCACAGGACCTACCGGGGAACCCGGACCAGCCGGACCACCCGGAGCGTCGGGAGCCCCGGGAGCTAGCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCgg                                                        <  2:3423967/140‑1 (MQ=14)

                                                                                      ccGGAGCGACGGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCCACGGGAGCAACCGGGGAATCCGTACCAGCCGGACCCACCGGAGCGCCGGGAGCCACAGGGGCtcagggg                                                       >  1:1570493/1‑134 (MQ=14)

                                                                                      ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:3459890/140‑1 (MQ=255)

                                                                                      ccGGAGCCCCGGGAGCCACAGGAGCCACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:2047623/140‑1 (MQ=14)

                                                                                              aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACGGGAGACACGGGAGCACCCGGGGCAGCCGGACAGGCCGGACCCACCGGAGCCACAGGAGCGACAGTAGCTACCGGGGACGCCgg                                               >  1:276516/1‑140 (MQ=17)

                                                                                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGACGCCGGa                                              >  2:1949356/1‑140 (MQ=255)

                                                                                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGCGCTACCGGGGAAGCCGGa                                              >  1:1903447/1‑140 (MQ=255)

                                                                                               cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACGGGAGCTACCGGGGAAGCCGGAGACGCGGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                              >  2:120800/1‑140 (MQ=14)

                                                                                                gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  1:742625/140‑1 (MQ=255)

                                                                                                gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  1:3248237/140‑1 (MQ=255)

                                                                                                    gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCAACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCCACCGGAGCAACCGGGGCGGCCGGagcagc                                         >  2:2795030/1‑140 (MQ=11)

                                                                                                        cAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgga                                     >  1:2627847/1‑140 (MQ=255)

                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCCCCGGAGCGACAGGAGAGAGAGGAGCGACAGGCCAAGCCGGACCTGCCggagcgagc                               >  2:1070901/1‑138 (MQ=14)

                                                                                                                aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacgggagccacagggg                                                               <  2:2076673/106‑3 (MQ=255)

                                                                                                                aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCggagcgacagg                             >  1:1823230/1‑140 (MQ=255)

                                                                                                                aCCGGCCAAGCCGGACCAGCCGGACCACCCGGACCGACGGGAGCCACGGGAGCTACCGGGGAACCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                             <  2:70375/140‑1 (MQ=17)

                                                                                                                 ccGGGGACGCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGCGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagga                            <  1:564122/140‑1 (MQ=17)

                                                                                                                 ccGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGAGGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagga                            <  1:921221/140‑1 (MQ=255)

                                                                                                                 ccGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagga                            <  1:1089205/140‑1 (MQ=255)

                                                                                                                     ggcagccggccccgccggaccACCCGGACCCCCCGGAGCCACGGGAGCTACCGGCGAAGCCGGACCAGCCGGACGACCCGGAGGGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                        <  2:662210/137‑1 (MQ=17)

                                                                                                                     ggcagccggcccagccggaccACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                        <  2:588596/137‑1 (MQ=255)

                                                                                                                     ggcagccggaccacccggaccACCCGGAGCGACGGGAGCCACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                        <  2:774118/137‑1 (MQ=14)

                                                                                                                      gAAGCCGGACCAGCCGGACCGACCGGAGCGACGGGAGCGAGCGGTGCTACCGGTGAAGCCGGACCTGCCGGACCAACCGGTGCGACCGGAGCGGCAGGCGCTGCCGGGGAAGCCGGGCCGGGCggggcggccggcgcggc                       >  2:3939354/1‑138 (MQ=9)

                                                                                                                      gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACCGGAGATACCGGGGACGCCGGACCAGCCGGACCGACCGGCGCGACCGGGGCGACCGCAGCTACCGGGCACCCCGGGCCGGCCggtgcgaccggcgcgag                       >  2:936746/1‑139 (MQ=14)

                                                                                                                       tagcgggaccagccggaccCACCGGCGCGCCGGGAGCCACGGGAGGTTCCGGGGCAGGCGGCCCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                      <  1:2871381/139‑1 (MQ=17)

                                                                                                                         gccggcccagccggaccaACCGGAGCGACGGGCGCCACGGGAGCGACCGGGGAACCCGGACCACCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  2:1608627/140‑1 (MQ=17)

                                                                                                                          ccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagga                   <  2:237505/140‑1 (MQ=255)

                                                                                                                                  gccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACTGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  2:3465989/140‑1 (MQ=255)

                                                                                                                                    cggaccaCCCGGACCCCCGGGAGCACCCGGAGCTACCGGGGCAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacaggagcgacaggagcgacaggag         <  2:4250/140‑1 (MQ=14)

                                                                                                                                      gaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt       <  2:871388/140‑1 (MQ=255)

                                                                                                                                      gaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt       <  2:1714099/140‑1 (MQ=255)

                                                                                                                                       accaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTa      >  1:2582135/1‑140 (MQ=255)

                                                                                                                                          aaCCGGAGCGACGGGAGCCACCGGTGCTACCGGGGAAGCCGGACCAGCCTGACCGACCGCACCGACCGGCGCGACAGGAGCTACCGGGGAAGCCGGACGAGCCGGAGCGACCGGAGCGCCCGGCGCGACTGGAGGTGCCg   >  1:684381/1‑140 (MQ=14)

                                                                                                                                           aCCGGAGCGCCGGGAGCCACGGGAGCTCCCGGGTAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg  <  1:2394383/140‑1 (MQ=255)

                                                                                                                                           aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg  >  2:1637385/1‑140 (MQ=255)

AGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607220‑607498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`