Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,857 C→A 8.6% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8570CA 8.6% 418.4 / 8.1 163intergenic (‑163/+43)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base C (82/62);  new base A (2/17);  total (84/79)
Fisher's exact test for biased strand distribution p-value = 1.28e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCT  >  NZ_CP034943/3996718‑3996994
                                                                                                                                           |                                                                                                                                         
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTACATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTAATAGCATTCTcc                                                                                                                                           >  2:3018041/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:1162206/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  2:803125/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  2:647868/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  2:3433989/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:372794/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:1246256/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAAAAGAAAGTCAATTGTAAATACGAACAATAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTAAAATCGTTTTCTCATCGCATTGTcc                                                                                                                                           >  1:2520943/1‑140 (MQ=255)
       gATTCTATTCAAACATATCAAGTTTATCAGTGAATAAACAGAAAGTCAATTGTAAATCCCCACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTAGAAGCTTTTTCTCATCGCATTGTCCTAAAAAg                                                                                                                                    <  2:4014391/140‑1 (MQ=255)
       gATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAg                                                                                                                                    >  1:407190/1‑140 (MQ=255)
       gATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAg                                                                                                                                    <  2:1542169/140‑1 (MQ=255)
         ttCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGAGTTTTCTCATCGCATTGTGCTAAAAAGCt                                                                                                                                  >  2:3115727/1‑140 (MQ=255)
              tttaaacaaaTCAAGTTTATCAGTGAAGTAACAGAAAGTCTATTGTAAATCCGATAATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  1:3651619/137‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  1:326646/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  1:3987684/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                             <  2:527012/140‑1 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTGGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                        >  2:4018248/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCAGTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                        >  1:2040409/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                        >  1:2456505/1‑140 (MQ=255)
                      aaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                     >  2:1711809/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                            >  2:434781/1‑140 (MQ=255)
                                aTCGGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCa                                                                                                           <  1:1726890/140‑1 (MQ=255)
                                aTCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCa                                                                                                           <  1:711641/140‑1 (MQ=255)
                                  cAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACg                                                                                                         <  2:3414807/140‑1 (MQ=255)
                                    gTGAAGAAACAGACAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCTTTTTCTCATCGCATTGTCCTAAAAACCTCGTGAAACTTTTATCCTTTTGCACGaa                                                                                                       <  2:825877/140‑1 (MQ=255)
                                      gaagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCTTGAAACTTTTATCATTTAGCACGaaaa                                                                                                     >  1:3548652/1‑140 (MQ=255)
                                      gaagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaa                                                                                                     >  1:2774330/1‑140 (MQ=255)
                                        agaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAag                                                                                                   <  2:774873/140‑1 (MQ=255)
                                        agaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAag                                                                                                   <  2:372794/140‑1 (MQ=255)
                                               taaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACATTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                            <  2:2360886/139‑1 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                            >  1:3231935/1‑140 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                            >  1:1495484/1‑140 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                            <  2:2463221/140‑1 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTACTGTAACCCAATATTCGTTCGCTTCCATTCGATTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                           <  2:1662452/140‑1 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                           <  2:185371/140‑1 (MQ=255)
                                                      aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                     <  2:417112/140‑1 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   <  1:1330784/140‑1 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   >  2:3465090/1‑140 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   <  2:563071/140‑1 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   <  2:3985300/140‑1 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   >  1:3829843/1‑140 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTAATGTAAATCAATATACGTTCGCTTCCAATCGTTTTACAAGCGTTTTCTCATCGCATGGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGGTACTTGCCTGaga                                                                                   >  1:3994986/1‑138 (MQ=255)
                                                        ttGTAAATCCGAACAGTAAATTAATGTAAATCCAGATTCGTTCGCTTCCACTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAACAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   <  1:2341256/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                  <  1:2440328/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                  <  1:2030915/140‑1 (MQ=255)
                                                           tAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATcc                                                                                <  2:254792/140‑1 (MQ=255)
                                                                  gAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGt                                                                         >  1:1996951/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  1:1248479/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  1:2829852/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        >  2:1527203/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        <  1:1117798/140‑1 (MQ=255)
                                                                              attttAATTCATTTTTCGTTCGCTACCATTGTTTTTAAAAGGTTTTTGTAATCGCTTTTTCCTAAAAAGCTCTTGAAACTTTTATCCTTTAGAACGAAAAAGTTTTACTTGCCTGATATCCCTTTTGTATTCATAAAAAc                                                             <  2:2736340/136‑1 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  2:869704/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:1925613/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  2:1767400/1‑140 (MQ=255)
                                                                                    aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                       >  1:1435770/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                      >  2:1420394/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                      >  2:200269/1‑140 (MQ=255)
                                                                                      tCACTATGCGTGCGCTTCCATTCGTTTTACAAGCGTTTTCTATTCGCATTGTACTAAAAAGCTCGTCAAACTTTTATCCTTTAGCCCGAAAAAGAGTTACTTGCCTGATATCCCTTTGGTATTAATAAAAACGACTCGcc                                                     <  2:3183156/140‑1 (MQ=255)
                                                                                          tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                                                 >  1:10575/1‑140 (MQ=255)
                                                                                          tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCACTTTTTTATTCATAAAAACGACTCGCCAAcc                                                 >  2:2990047/1‑140 (MQ=255)
                                                                                              cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCTTTACAACGACTCGCCATCCGTcc                                             >  2:2053905/1‑140 (MQ=255)
                                                                                              cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGACAAACGTcc                                             >  2:1847627/1‑140 (MQ=255)
                                                                                                ttcgTTTCCATTGTTTTTAAAAGCGTTTGCACATCGCTTGTTCATAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGTGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCgg                                           <  2:3659412/140‑1 (MQ=255)
                                                                                                ttcgGTTCAATTCGTTTTACATGCGTTTTCTCAACGATTTGTCCTAAAAAGCTGGTGAAACTTTTATCCTTTAGCACCAAAAAGAGTTAGTGGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                           <  2:1607064/140‑1 (MQ=255)
                                                                                                ttcgCTTCCATTCTTTTTAAAAGCTTTTTCTCATCGAATTGTCCAAAAAACCTCGTGAAACTTTTATCCTTTAGCACTAAAAATAGTTACTGGCCTGATATCCCTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCgg                                           <  2:1089634/140‑1 (MQ=255)
                                                                                                ttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                           >  1:701549/1‑140 (MQ=255)
                                                                                                ttcgCTTCCATTCGTTTAACAAGCTTTTTCTCATCGAATTGTCATAAAAAGATCTTTAAATTTTTATCCTTTCGCACGAAAATGAGTTACTTGCGTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                           <  2:3103274/140‑1 (MQ=255)
                                                                                                tgcgCTTCCTTTCGTTTTACAAGCTTTTTCTCATCGCATTGTCCTAAAAAGCTGGTTAAACTTTTATCCTTTAGCACCAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                           <  2:2830554/138‑1 (MQ=255)
                                                                                                 tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                   <  1:871943/99‑1 (MQ=255)
                                                                                                 tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGa                                          >  1:1089634/1‑140 (MQ=255)
                                                                                                 tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGa                                          >  1:3900704/1‑140 (MQ=255)
                                                                                                  cgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAt                                         >  1:529649/1‑140 (MQ=255)
                                                                                                  cgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAt                                         >  2:352995/1‑140 (MQ=255)
                                                                                                   gCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATc                                        >  2:162487/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCCTGGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCGTTTAGCTCGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTAGGCATAAACACGTGTCGCCAACCGTCCGGATcc                                       >  1:2848016/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                       >  1:928152/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                       >  2:3356019/1‑140 (MQ=255)
                                                                                                       caaTTGGTTTTACAAGCGTTTGCCCTTCGAATTGTCCTAAAACGCTCGTGAAACTTTTATCCTTTCGCACTAAAAATAGTTCGTTGCCTGATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCctct                                    <  2:3241594/138‑1 (MQ=255)
                                                                                                         aTTCTTTTTAAAAGGGTTTTCTCATCGAATTGTCCTAAAAAGCTCGTGAAACTTTTATCATTTAGCCCGAAAAAGTGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTAt                                  <  2:468934/140‑1 (MQ=255)
                                                                                                               tttACAAGCGTTTTCTCATCGCATTGTCCTCAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAACAAGAGTTACTTGCCTGATATCCCTTTTGTATTCACTACAAAGACTCGCCAACCGTCCGGATCCGCTTTCGGCAc                            >  2:2327481/1‑140 (MQ=255)
                                                                                                               tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTGTTATCCTTTAGCACGACAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                            >  2:887350/1‑140 (MQ=255)
                                                                                                                       ggTTTTCTCATCTATTTGTCAAAAAAACCTCTTGAAAATTTTAACCTTTAGAACTAAAAATAGTTACTTGCCTGATATCCATTTTGTATGAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:1495484/139‑1 (MQ=255)
                                                                                                                       ctttttCTAATCTCTTTGTCATAAAAAGATCTTTAAAATTTTATCCTTTAGCACTAAAAAGTGTTAGTTGCATGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:2435892/138‑1 (MQ=255)
                                                                                                                       cGTTTTCTCATCTCTTTGTCCTAAAAAGCGCGTCAAAATTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATAGCCCTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:1330583/140‑1 (MQ=255)
                                                                                                                       cGTTTTCTCATCGCATTGTCATAAAAAGCTGGTTAACATTTTATCCTTTAGCCCCAAAATGTGTTTCTTGCCTGATATCCCTTTTTTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:661471/140‑1 (MQ=255)
                                                                                                                       cGTTTTCAAATCGAATTGTCCTAACACGCTCGTGAAACTTTTCACCTTTACCACGAAAAAGAGTTACTTGCCTGATAACCCTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  1:968957/140‑1 (MQ=255)
                                                                                                                       cGTTTTAACATCGCTTTGTCCTAAAACGCTCGTTAAACTTTTATCCTTTCGCACCAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:1404224/140‑1 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTGTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                  >  2:1176360/1‑140 (MQ=255)
                                                                                                                         ttttCTCATCGAATTTTCATAAAAAGCTCTTTAAATTTTTATCCTTTAGAACGAAAAATTGTTAGTTGCATGATATCCATTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                  <  2:3231935/140‑1 (MQ=255)
                                                                                                                          tttCTCTTCGATTTTTCATAAAAAGCTCGTTAAATTTTTATCATTTAGAACTAAAAAGAGTTAGTTGCGTTATTTCCGTTTTTTTTTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                 <  1:3115727/140‑1 (MQ=255)
                                                                                                                          tttCTCATCGCTTTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                 <  2:2752767/140‑1 (MQ=255)
                                                                                                                          ttgctcAGCGCAGTGTCCTACACACGTCGTGAAACTTTTAGCCTTTCGCACGAAAACGCGTTAGGTCCCTTATCTCCCTTGTGTATAAATAAACACGACTCGCCAACCCTCCGGATCCTCTATCGTCACGCCTCCAGGGc                 <  1:3973511/137‑1 (MQ=255)
                                                                                                                                 tCGCTTTGTCCTAAAAAGCTGGTCAAACTTTTATCCTTTACCACTAAAAAGTGTTAGTTGCCTTATATCCCTTTTGTATTAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa          <  1:844462/140‑1 (MQ=255)
                                                                                                                                 tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa          >  2:143260/1‑140 (MQ=255)
                                                                                                                                  cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc         >  2:3835585/1‑140 (MQ=255)
                                                                                                                                  cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc         >  1:3033044/1‑140 (MQ=255)
                                                                                                                                  cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc         >  2:3629014/1‑140 (MQ=255)
                                                                                                                                  cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAAACGTCCGGATACTCTATCGTCACTCCTCAAAGCCTCCAGTAc         >  2:960766/1‑140 (MQ=255)
                                                                                                                                         tCCAAAAACGCGCGTGAAATTTTTATCCTTTCCCACCAAAATGAGTTACTTGCCTTATATCCCTTTTGTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct  <  1:1526438/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCT  >  NZ_CP034943/3996718‑3996994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: