BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,996,927	C→A	6.6%	M131I (ATG→ATT)	EO946_RS20530 ←	VOC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,996,927	0	C	A	6.6%	661.4 / 3.5	270	M131I (ATG→ATT)	EO946_RS20530	VOC family protein
Reads supporting (aligned to +/- strand): ref base C (151/90); new base A (3/26); total (154/116)
Fisher's exact test for biased strand distribution p-value = 7.11e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

AATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGCA  >  NZ_CP034943/3996796‑3997066

attttAATTCATTTTTCGTTCGCTACCATTGTTTTTAAAAGGTTTTTGTAATCGCTTTTTCCTAAAAAGCTCTTGAAACTTTTATCCTTTAGAACGAAAAAGTTTTACTTGCCTGATATCCCTTTTGTATTCATAAAAAc                                                                                                                                     <  2:2736340/136‑1 (MQ=255)

  tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                                   >  1:1125757/1‑140 (MQ=255)

  tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                                   >  2:869704/1‑140 (MQ=255)

      aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                                                                                               >  1:1435770/1‑140 (MQ=255)

      aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                                                                                               >  2:3549462/1‑140 (MQ=255)

        tCACTATGCGTGCGCTTCCATTCGTTTTACAAGCGTTTTCTATTCGCATTGTACTAAAAAGCTCGTCAAACTTTTATCCTTTAGCCCGAAAAAGAGTTACTTGCCTGATATCCCTTTGGTATTAATAAAAACGACTCGcc                                                                                                                             <  2:3183156/140‑1 (MQ=255)

            tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                                                                                                                         >  1:1463742/1‑140 (MQ=255)

            tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                                                                                                                         >  1:10575/1‑140 (MQ=255)

            tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCACTTTTTTATTCATAAAAACGACTCGCCAAcc                                                                                                                         >  2:2990047/1‑140 (MQ=255)

                cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCTTTACAACGACTCGCCATCCGTcc                                                                                                                     >  2:2053905/1‑140 (MQ=255)

                cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGACAAACGTcc                                                                                                                     >  2:1847627/1‑140 (MQ=255)

                  ttcgGTTCAATTCGTTTTACATGCGTTTTCTCAACGATTTGTCCTAAAAAGCTGGTGAAACTTTTATCCTTTAGCACCAAAAAGAGTTAGTGGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                                                                                                   <  2:1607064/140‑1 (MQ=255)

                   tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGGTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGa                                                                                                                  >  2:3111576/1‑140 (MQ=255)

                    cgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAt                                                                                                                 >  2:352995/1‑140 (MQ=255)

                    cgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAt                                                                                                                 >  2:3798330/1‑140 (MQ=255)

                    cgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAt                                                                                                                 >  2:1738240/1‑140 (MQ=255)

                     gCTCCCAGTCGTTTTACAAGCGTTTTCTCCTCGAATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTCGCACTAAAAAGACTTACTTGCCTCATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATc                                                                                                                <  2:3488749/140‑1 (MQ=255)

                           aTTCTTTTTACAAGCGTTTTCTCTTGGCATTGTCCTAAAAAGCTCGTTAAATTTTTATCATTTAGCACGAAAAAGATTTAATTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTAt                                                                                                          <  2:468935/140‑1 (MQ=255)

                                ttttACATGGTTTTTATATTCGAATTGTCATAAAAATCTGGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                                                                                                     <  2:1162206/140‑1 (MQ=255)

                                ttttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                                                                                                     <  2:2829852/140‑1 (MQ=255)

                                tttgACAAGTGTTGTCTCTTCGAATTGTCCTAAAAAGCTCGGGAAATTTTTATCAGTGAGCACCAAAAAGTGTTACTTGCGTGATATCCCTGGGGTTTTCTTAAACACGACTCGCCCACCGTCCGGATCCTCTCTCGTCa                                                                                                     <  2:3333972/140‑1 (MQ=255)

                                 tttACAAGCGTTTTCTCATCGCATTGTCCTCAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAACAAGAGTTACTTGCCTGATATCCCTTTTGTATTCACTACAAAGACTCGCCAACCGTCCGGATCCGCTTTCGGCAc                                                                                                    >  2:2327481/1‑140 (MQ=255)

                                        gCGTTTTCTAATCGAATGTTCATAAAAAGCTGTTTAAATTTTTATCCTTTAGCACTAAAAATTTTTATTTGCCTGATAGCCATTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                                                                                             <  1:922326/140‑1 (MQ=255)

                                         ggTTTTCTCATCTATTTGTCAAAAAAACCTCTTGAAAATTTTAACCTTTAGAACTAAAAATAGTTACTTGCCTGATATCCATTTTGTATGAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                            <  2:1495484/139‑1 (MQ=255)

                                         ggTTTGCTCAGCGATTTGTACTAAAAAGTTGTTTAATTTTTTATGCTTTACCAACAAAAATATTTTCTTGCCTTATTTCCTTTTTTTATTAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                            <  1:2990047/139‑1 (MQ=255)

                                         ctttttCTAATCTCTTTGTCATAAAAAGATCTTTAAAATTTTATCCTTTAGCACTAAAAAGTGTTAGTTGCATGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                            <  2:2435892/138‑1 (MQ=255)

                                         cGTTTTCTCATCGCATTGTCATAAAAAGCTGGTTAACATTTTATCCTTTAGCCCCAAAATGTGTTTCTTGCCTGATATCCCTTTTTTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                            <  2:661471/140‑1 (MQ=255)

                                         cGTTTTCAAATCGAATTGTCCTAACACGCTCGTGAAACTTTTCACCTTTACCACGAAAAAGAGTTACTTGCCTGATAACCCTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                            <  1:968957/140‑1 (MQ=255)

                                         cGTTTTAACATCGCTTTGTCCTAAAACGCTCGTTAAACTTTTATCCTTTCGCACCAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                            <  2:1404224/140‑1 (MQ=255)

                                           ttttCTCATCGAATTTTCATAAAAAGCTCTTTAAATTTTTATCCTTTAGAACGAAAAATTGTTAGTTGCATGATATCCATTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                                                                                          <  2:3231935/140‑1 (MQ=255)

                                            tttCTCATCTCTTTGTACAAAAAAGCTCTTTAAATTTTTATCCTTTACCACTAAAAAGAGTTACTTGCGTTATATCCCTTTTTTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                                                         <  1:3225116/140‑1 (MQ=255)

                                                   tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                                                                  >  2:143260/1‑140 (MQ=255)

                                                    cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAAACGTCCGGATACTCTATCGTCACTCCTCAAAGCCTCCAGTAc                                                                                 >  2:960766/1‑140 (MQ=255)

                                                       aTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTCGCACCAAAAAGAGTTACTTGCCGGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACggg                                                                              <  2:41169/140‑1 (MQ=255)

                                                          gTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtc                                                                           >  1:3652500/1‑140 (MQ=255)

                                                              tAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAg                                                                       >  1:828249/1‑140 (MQ=255)

                                                                aaaaGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAt                                                                     >  2:899284/1‑140 (MQ=255)

                                                                  aagatggTGAAACTTTTATCCTTTAGAACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                   <  2:1636045/134‑1 (MQ=255)

                                                                     ctcttcaaaaTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                <  2:10575/134‑1 (MQ=255)

                                                                     cTCGTGAAAGTTTTATCCTTTAGCACTAAAAAGTGTTAGTTGCCTGATATCCCTTTTGTATTAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                <  1:44377/140‑1 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAATGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCTAc                                                                >  1:3851558/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:3957554/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:2075245/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:184646/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:2477711/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:675628/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:3300057/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:2204060/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:3490273/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:67479/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:3826653/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:1191636/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:1257035/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:493886/1‑140 (MQ=255)

                                                                     cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTCTCGTCACGCCTCCATGGCGCCAGGACGGGTTCTCAGATTCacc                                                                >  2:2145025/1‑138 (MQ=255)

                                                                      tttttaaaTTTTTATCCTTTACCACTAAAAAGAGTTAGTTGCCTTATATCCATTTTTTATTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                                                               <  1:1404513/135‑1 (MQ=255)

                                                                       gtttaaaTTTTTATCTTTTACCCCGAAAATGATTTATTTGCGTGATATCCCTTTTTTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACct                                                              <  1:2849708/136‑1 (MQ=255)

                                                                            aaCTTTTATCCTTTCCCACTAAAAAGATTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                                         <  2:576558/140‑1 (MQ=255)

                                                                            aaCTTTTATCATTTAGAACGAAAAATTGTTAATTTCATCTTATCCCTTTTGTATAAATAAAAACGACTCGCCAAACTTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                                         <  2:841504/140‑1 (MQ=255)

                                                                             atttttATCTTTTTTCACTAAAAATTTTTATTTGCCTTATTTCCTTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGAt                                                        <  1:2749526/138‑1 (MQ=255)

                                                                             aattttATCCTTTCGCACGAAAAATAGTTACTTGCCTTATATCCCTTTTGTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGAt                                                        <  2:744452/138‑1 (MQ=255)

                                                                               ttttATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAg                                                      >  2:2466098/1‑140 (MQ=255)

                                                                                   aTCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATACAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa                                                  <  1:3730933/140‑1 (MQ=255)

                                                                                         tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                            >  1:2402819/1‑140 (MQ=255)

                                                                                         tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                            >  1:3475825/1‑140 (MQ=255)

                                                                                         tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                            >  1:565898/1‑140 (MQ=255)

                                                                                          cgCACGAAAAAGAGTTACTTGCGGGATACCCGTTTGTTATACATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  1:3549462/139‑1 (MQ=255)

                                                                                          cccACGAAAAAGAGTTAATTGCCTGATATCCCTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  2:1531002/138‑1 (MQ=255)

                                                                                          agcaacaaaaaGATTTCCTGCCCTGATAACCGTTTTTTATCAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  1:162487/134‑1 (MQ=255)

                                                                                          aGCACGAAAAAGTGTTACTTGCCTGATATCCCTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  2:2774330/140‑1 (MQ=255)

                                                                                          aGCACCAAAAATCGTTAGTGGCCTGATATCCCTTTGGAATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  1:200269/140‑1 (MQ=255)

                                                                                          aGCACCAAAAAGATTTACTTGCCTCATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  2:3658584/140‑1 (MQ=255)

                                                                                                 aaaaGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATACTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCACTGGATAGCCCATCTGTTTTAGCttt                                    >  2:3259333/1‑140 (MQ=255)

                                                                                                 aaaaGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTCTCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCttt                                    >  2:2625546/1‑140 (MQ=255)

                                                                                                         tccTTGCCCGATATCCATTTTGTTGTCATAAAAACGACTAGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGAt                            <  2:1984266/138‑1 (MQ=255)

                                                                                                           ctggCCTGATATCCCTTTTTTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCg                          <  1:3184588/137‑1 (MQ=255)

                                                                                                           ctggCCTGATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCg                          <  1:2678079/137‑1 (MQ=255)

                                                                                                           cgggCCTGATCTCCGTTTTGTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCg                          <  1:339352/137‑1 (MQ=255)

                                                                                                           cTTGCCTGAGATCCCTTTTGTTTTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCg                          <  2:3832797/140‑1 (MQ=255)

                                                                                                            ttGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGc                         >  2:1274026/1‑140 (MQ=255)

                                                                                                                cTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTc                     >  2:1147201/1‑140 (MQ=255)

                                                                                                                cTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTc                     >  2:3872954/1‑140 (MQ=255)

                                                                                                                cTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTc                     >  2:1177441/1‑140 (MQ=255)

                                                                                                                cTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTc                     >  2:2848524/1‑140 (MQ=255)

                                                                                                                     acagcTTTTGTATTCATAAAAACGAATCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGATGTCAGttt                >  1:3900950/5‑140 (MQ=255)

                                                                                                                       cccTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGt              <  1:844778/140‑1 (MQ=255)

                                                                                                                       cccTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGt              <  1:2204060/140‑1 (MQ=255)

                                                                                                                          ttttGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  1:3985582/108‑1 (MQ=255)

                                                                                                                          ttttGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           >  2:3985582/1‑108 (MQ=255)

                                                                                                                          ttttGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTg           >  2:2663836/1‑140 (MQ=255)

                                                                                                                          ttttGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTg           >  2:181290/1‑140 (MQ=255)

                                                                                                                          ttttGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTg           >  2:1340121/1‑140 (MQ=255)

                                                                                                                          ttttGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCCTCACGCCTCCATCGCTCCAGTACGCGTTCTCAGATTCAAACTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTg           >  2:2384875/1‑140 (MQ=255)

                                                                                                                            ttGTATTCATAAAAACGACTCGCCAACCGTCCGGATCGTCTATCGTCACGACTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTGTGATGTTATGGCTGTCAGTTTGTGTGcc         >  1:2770820/1‑139 (MQ=255)

                                                                                                                            ttGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCa         >  1:1968484/1‑140 (MQ=255)

                                                                                                                             tGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCAt        >  2:3294466/1‑140 (MQ=255)

                                                                                                                             tGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAAGGGTTCTCAGATTCAACCTCTTGATAGCCCATGAGGTGTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGAAt        >  2:3217447/1‑140 (MQ=255)

                                                                                                                                 ttCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCgg    <  2:3280696/140‑1 (MQ=255)

                                                                                                                                 atCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACTCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCgg    <  2:850545/139‑1 (MQ=255)

                                                                                                                                   cATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGca  <  1:4018248/140‑1 (MQ=255)

AATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGCA  >  NZ_CP034943/3996796‑3997066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`