Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,638 G→C 9.3% A161P (GCC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6380GC 9.3% 287.2 / 3.3 137A161P (GCC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (59/63);  new base C (6/7);  total (65/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGG  >  NZ_CP034943/607499‑607770
                                                                                                                                           |                                                                                                                                    
ggAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAg                                                                                                                                      >  1:768044/1‑140 (MQ=255)
ggAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCAGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAg                                                                                                                                      >  1:737555/1‑140 (MQ=255)
 gccccgggACCAGCCGCAGCGACGGGAGCCACCGGCGCGACCGGGGAAGCCGCACCAGCCGGCGCCACGGGAGCCTCAGGGGCTTCTGGTGAAGCCGGACCTGCGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                     <  2:3545319/134‑1 (MQ=17)
 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                     >  2:3222806/1‑140 (MQ=255)
 gAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCTGCCGGAGCCACGGCAGCCACTGGCTCTCGCGCGGCACCCGGACCGACCGGAGCGACAGGAGATCCGGGGTACGGCGGACCAGc                                                                                                                                     >  2:247396/1‑140 (MQ=9)
  aaGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                    <  2:2819085/140‑1 (MQ=255)
    gcgggACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCTCAGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                  <  2:817961/137‑1 (MQ=255)
    ccggctgcAGCGGGAGCCACGGGAGCGCCCGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                  <  2:1530518/133‑1 (MQ=11)
    ccaggACCCGCGGGAGGCACGGGAGCCACAGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                  <  2:2151345/137‑1 (MQ=17)
     ccggcccAGCCGGCGCGACGGGAGCGCCAGGAGCCACCGGGGACGCCGGACCAGCCGGCGCCACGGGCGCCAGTGGGGCATGTGGGGTTGCCGGACCAGGCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                                                 <  1:26082/135‑1 (MQ=14)
          cccccgcgagcgccgGGCGCGACAGGAGCTCCCGGGGCCGCCGGCCCAGCGGGCGACACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGac                                                                                                                            <  2:387894‑M1/133‑3 (MQ=255)
          ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGac                                                                                                                            <  2:2372539‑M1/140‑3 (MQ=255)
            aGCTGGAGCGACGGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCCGCGGGGGCAACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAg                                                                                                                          <  1:1952093/140‑1 (MQ=17)
             tcTGGAGCGACGGGAGCTCCCGGAGCTCCCGCAGAACCCGGACCACCCGGCGCCACGGGAGCCACAGGCGCTACTGGGGAAGCGGGACCCCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                         <  1:1419587/139‑1 (MQ=17)
             gccgcaGCGCCGGGCGCGACAGCAGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                         <  1:3206695/135‑1 (MQ=17)
             gCTGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACTGGGGCTAGTGGGTAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                         <  1:1070901/140‑1 (MQ=17)
             gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCACCCGCAGCGACAgg                                                                                                                         >  2:2808609/1‑140 (MQ=255)
             gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAgg                                                                                                                         <  1:2064359/140‑1 (MQ=255)
             gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGCGGCCACGGGAGCCACAGGGGCTCCTGGCGAAGCCGGACCAGCGGGAGCGCCAGGAGCTCCGGGGGATGACGGTCCAGCCGGAGCGCCAgg                                                                                                                         >  2:2428006/1‑140 (MQ=17)
             ccgggcgCGACGGGAGCGACAGGAGCTACCGGCGCCCCCGGACCACCCGGGGCCACGGGAGCCCCAGGGGCAACGGGGGACGCGGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                         <  1:3867920/134‑1 (MQ=14)
              cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTGGCTGGGAAGCCGGACACGCCGGAGCGacggga                                                                                                                        >  1:2082335‑M1/1‑134 (MQ=255)
              cTGGAGCGACGGGAGCGACAGGAGCTACCGCGCAAGCCGCACCAGCCGGGGCCACTGGAGCCACAGGGGCTACTGGCGAAGCCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGaccgga                                                                                                                        >  1:3731546‑M1/1‑134 (MQ=255)
                  cgcttcGGGAGCCCCAGGAGCTACCGGGGCAGCCGGAGCAGCGGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                    <  1:2040701/135‑1 (MQ=14)
                  aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCCCCGGAGCCACAGGGGCTACTGGGGAAGACGGACCAGCCGGACCGACAGGAGCTACCGGGGAAGCCGGACCCGGCGGCGCGACACGAGCtc                                                                                                                    >  1:2153997/1‑139 (MQ=17)
                  aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                    >  1:532091/1‑140 (MQ=255)
                  aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTa                                                                                                                    >  1:459913/1‑140 (MQ=255)
                      aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCGACAGGGGCTACTCGTGACGCCGGACCAGCCGGAGCGCCAGGAGCAACCTGGGACGCCGGACCAGCCGGAGCGACAGGAGcgcgcgg                                                                                                                >  1:3778292/1‑134 (MQ=14)
                      aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCtggcgc                                                                                                                >  1:1530518/1‑135 (MQ=255)
                      aCGGGAGCGACAGGAGCTACCGGAGAAGCCGGACCAGCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTCCCgg                                                                                                                >  1:3687773/1‑140 (MQ=14)
                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCGACTGGGGAAGGCGGACCAGCGGGAGCGACCGGAGCTACGGGGGACGCCGGACCAGCCGGTGCGacgggagcgcccggg                                                                                                               >  1:2940155‑M1/1‑125 (MQ=255)
                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCggg                                                                                                               >  1:2784476/1‑140 (MQ=255)
                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGATACCggg                                                                                                               >  1:100359/1‑140 (MQ=255)
                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCggg                                                                                                               >  1:2512881/1‑140 (MQ=255)
                        gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                              >  1:2245395/1‑140 (MQ=255)
                         ggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCgccgc                                                                                                             >  1:2610015/1‑136 (MQ=255)
                           aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                           <  1:835147/140‑1 (MQ=255)
                             cGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                                                                                         <  2:678109/140‑1 (MQ=255)
                             cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                                                                                         <  2:1430455/140‑1 (MQ=255)
                                        cccGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                              <  1:1269749/139‑1 (MQ=255)
                                        aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGCCCacacgg                                                                                              >  1:666187/1‑135 (MQ=17)
                                        aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCAGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                              >  1:210688/1‑140 (MQ=255)
                                             ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCACCCGGAGCGACAGGAGCACCCGGGGCCGCCGGACCAGCCGCAGCgc                                                                                         >  1:2269185/1‑139 (MQ=17)
                                              gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                        <  1:1153347/140‑1 (MQ=255)
                                              gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                        <  1:361856/140‑1 (MQ=255)
                                              gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                        <  1:2294234/140‑1 (MQ=255)
                                                 gCCGGACCAGCCGGGGCCCCGGGCGCCTCAGGGGCTACTGGCGAAGCCGGACCAGCCGGAGCCACAGGAGCTCCCGGCGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                     <  1:1034162/140‑1 (MQ=17)
                                                   cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCCACCGGAGCGACAGGAg                                                                                   >  1:1662616/1‑140 (MQ=255)
                                                           ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACggga                                                                           >  2:1123606/1‑139 (MQ=255)
                                                           ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCAGGAGCGACAGGAGCGACAGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCAAcagcg                                                                           >  1:3392722/1‑136 (MQ=17)
                                                                    cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGa                                                                  >  1:924502/1‑140 (MQ=255)
                                                                    cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                           >  1:885984/1‑131 (MQ=255)
                                                                    cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                           <  2:885984/131‑1 (MQ=255)
                                                                    cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAGGCCGGa                                                                  >  2:1235956/1‑140 (MQ=255)
                                                                    cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                  <  1:4004087/140‑1 (MQ=255)
                                                                         gCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                             <  2:1636533/140‑1 (MQ=255)
                                                                          ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGa                                                                                    <  2:2772481/116‑1 (MQ=255)
                                                                              aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                        <  2:2245395/140‑1 (MQ=255)
                                                                                  gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAGCCCGGACCAGCCGGAGctcc                                                    >  2:3950316/1‑137 (MQ=14)
                                                                                    cccTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACGGGCGAAGCCGGAGCAGCCGGAGCCCCAGGAGCTCCGGGCGACGCAGGAGCTACCGGAGCGACAGGAGCTACCGGCGAACCCGGACCAGCCGGAGCGACAg                                                  <  1:3634389/138‑1 (MQ=14)
                                                                                    caCTGCGGACGCCGGACCCGCCGGCGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAg                                                  <  1:2669656/139‑1 (MQ=14)
                                                                                     ccgggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                 <  1:3939354/137‑1 (MQ=255)
                                                                                     aCTGGGGAAGCCGGACCAGCCGGGGCGAAAGGACATAGCTGGGAAGCCGGACCACCCGGCGCGACTAGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCAGCCGGcgcgtgggg                                                 >  2:3949107/1‑135 (MQ=11)
                                                                                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCGACCGGGCCGCCGGCGCAGCCGGAGCGACAGGa                                                >  2:1643770/1‑140 (MQ=14)
                                                                                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGAGCGACCGGAGCGCCCGGGGCAGCCGGACAAACCGGAGCgcccgg                                                 >  2:609040/1‑135 (MQ=11)
                                                                                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGCGAAGCCGGACCAGCCGGAGCTACCGGAGCGACCGGCGCGCCCGGAGCAGCCGGAgacgcagc                                                 >  2:3471282/1‑133 (MQ=11)
                                                                                     aCTGGGGAAGCCGGACCAGCCGGAGCGACACGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGTCCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                 <  1:3939355/140‑1 (MQ=255)
                                                                                       cggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                               <  2:3008840/139‑1 (MQ=39)
                                                                                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGAGACGCAGGAACAACCGCAGCGACATGAGCTACAGGGGACTCAGGACCAGCCGGAGCGCCAGGAGCTAc                                           >  1:2773157/1‑140 (MQ=14)
                                                                                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGGCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGTAGCTCAAGGGGAAGCCGGACCAGCCGGAGCGACACGAGCTccc                                          >  1:344714/1‑140 (MQ=14)
                                                                                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCGAcc                                          >  1:803072/1‑140 (MQ=255)
                                                                                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                          >  1:1596108/1‑140 (MQ=255)
                                                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                                  >  1:3566995/1‑66 (MQ=35)
                                                                                              gCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGAGCAGCCTGAGCGACATCAGCTACCGGGCAAGCCGGACCAACCGGTGCGAGAGGAGCTACCGCGGAAGCAGGACCAGCCGGGGCGACAGGAGCTACCgg                                        >  1:3485701/1‑140 (MQ=14)
                                                                                               ccGGACCCGCAGGAGCGAGTTGAGCTACCGGGGTTGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                       <  1:3340074/140‑1 (MQ=14)
                                                                                                   aCCCGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGTGGAAGCCCGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                   <  1:931463/140‑1 (MQ=255)
                                                                                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGacg                                   >  1:1868117/1‑138 (MQ=14)
                                                                                                        ccGGCGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                              <  1:2505022/140‑1 (MQ=255)
                                                                                                        ccGGAGCGCCTGGAGCCTCGGGGGACCCCCGACCAGACGGAGCCACAGGAGCTCACGGTGAACCCGGACCAACCGGAGCCACAGGAGCTACAGGCCAAGCGGGACCAGCGGGAGCCACAGGAGCTACCGGGGAAGCCGGa                              <  1:1799264/140‑1 (MQ=9)
                                                                                                        ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                              >  1:982882/1‑140 (MQ=255)
                                                                                                        ccGGAGCCACCGGAGCTCCGGGGGAAGCGGGACCAGCCGGAGCGACAGCAGCTTCCCGCGAATCCGGACCAACCGGAGCGACAGGCGCTACCGGCGAAGCCGGACAAGACGGACCGACAGGAGCTACCGGGGAAGCCGGa                              <  1:672752/140‑1 (MQ=11)
                                                                                                          ggcgccaCAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                            <  2:944524/134‑1 (MQ=11)
                                                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAGGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                          <  1:1235956/140‑1 (MQ=255)
                                                                                                                 cAGGAGCGCCCGGGGACGCCGGACCCGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                     <  1:3874389/140‑1 (MQ=14)
                                                                                                                 cAGGAGCGACCGGGGACGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                     <  1:774268/140‑1 (MQ=255)
                                                                                                                  aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGacgggagccaca                                                                                                                  <  1:3309737‑M1/46‑13 (MQ=255)
                                                                                                                       cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGTAGCGACAGGGGACGACGGACCAACCGGAGCCACg               >  1:113854/1‑140 (MQ=14)
                                                                                                                         aCCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg             <  2:768044/140‑1 (MQ=255)
                                                                                                                         aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg             <  2:349043/140‑1 (MQ=255)
                                                                                                                           cGGGGACGCCGCACCACCCGGAGCGacgggagcctcaggggctcccggggacggcggaccagccggagcgacaggagctaccggggaagccggaccacccggagcgacgggagccacaggggctactggggacgtcggac           <  2:666187‑M1/140‑116 (MQ=255)
                                                                                                                           cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAACAGGAACAGCCGGAGCGACAGTAGCAACCGACGAATCCAGACCACACGCAGAGACAGGac           >  1:1026467/1‑139 (MQ=11)
                                                                                                                           cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCAGCCGGAGCGACCGGAGCCACCGGGGCAGCCCGAGCAGGCGGAGCGACAGGAGCGACAGGGGACGCCGGACCCACCGGAGCGACCGGAg           >  2:1902338/1‑140 (MQ=11)
                                                                                                                             ggcaaGCTGGACCACCGGGAGCCCCAGGAGATACGGGCGACCCCGGACCTACCGGCGACCCAGGACCTCCCGGGGATTCCGGACCCCCCGGAGCTACAGGTGCTCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGcc         <  2:966804/137‑1 (MQ=2)
                                                                                                                               gacgccgGCCCAGCCGGAGCCaccgccgccacagccgctaccggggacggcggaccagccggcgcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagc       <  2:1868117‑M1/137‑120 (MQ=255)
                                                                                                                               gacgCCGGACCCGCCGTATCGACAGCCGCGACCGCGGAAGCAGGACCAACCGGAGCTACAGGAGCGCCCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac       <  2:1967000/137‑1 (MQ=14)
                                                                                                                                aaGCCGGACCCGCCGGCGCCAGAGGCGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca      <  2:390298/140‑1 (MQ=14)
                                                                                                                                 aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACTCCGGACCAGCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg     >  1:2962203/1‑140 (MQ=255)
                                                                                                                                  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCCGGCCCAGCCGCAGCGACAGGAGCGCCCGGCGCCGCCGGACAAGCGGGTGCGCCGGGCGCGACAgg    >  2:170225/1‑140 (MQ=11)
                                                                                                                                  cccGGACCCCCCGGAGCGCCAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCCCCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAgg    <  1:1431403/139‑1 (MQ=14)
                                                                                                                                    cgcaacAGCCGGAGCGACAGGCGCTACCGGTGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg  <  2:248733/135‑1 (MQ=14)
                                                                                                                                    cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACTCCGGACCAGCCGGAGCGACTGGAGCAACCGGGGCAGCCGGACCAACCGGAGCGACGGGATCCACAggag  >  2:1998197/1‑138 (MQ=14)
                                                                                                                                           |                                                                                                                                    
GGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGG  >  NZ_CP034943/607499‑607770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: