BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	30.5%	E181D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	30.5%	147.1 / 100.2	166	E181D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (53/57); new base C (13/35); total (70/96)
Fisher's exact test for biased strand distribution p-value = 1.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 168 total aligned reads displayed.

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAG  >  NZ_CP034943/607567‑607839

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGa                                                                                                                                       >  1:924502/1‑140 (MQ=255)

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAGGCCGGa                                                                                                                                       >  2:1235956/1‑140 (MQ=255)

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                       >  2:1273872/1‑140 (MQ=255)

cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGATCGGGGACTCCGGACCAACCGGAGCTACAGGAGCAAACGGGGATGCGGGa                                                                                                                                       >  1:622025/1‑140 (MQ=14)

 cggggaagccggaccaaccggagcgacgggagccacaggggctagtggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGAc                                                                                                                                      <  2:3917748‑M1/13‑1 (MQ=255)

    ggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAg                                                                                                                                   <  1:2567661‑M1/16‑1 (MQ=255)

        gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgccaggagctaccggggaagccggaccaaccggcgcgacgggagccacaggggctcctGGGGACGTCGGACCAGCCgg                                                                                                                               <  2:3662474‑M1/20‑1 (MQ=255)

        gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGAAGGCGGAGCAGCCgg                                                                                                                               <  2:1587696‑M1/20‑1 (MQ=255)

             accaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacgggggctactGGGGACGTCGGACCAGCCGGAGCGa                                                                                                                          <  2:313225‑M1/25‑1 (MQ=255)

              gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAGCCCGGACCAGCCGGAGctcc                                                                                                                         >  2:3950316/1‑137 (MQ=14)

                cccTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACGGGCGAAGCCGGAGCAGCCGGAGCCCCAGGAGCTCCGGGCGACGCAGGAGCTACCGGAGCGACAGGAGCTACCGGCGAACCCGGACCAGCCGGAGCGACAg                                                                                                                       <  1:3634389/138‑1 (MQ=14)

                caCTGCGGACGCCGGACCCGCCGGCGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAg                                                                                                                       <  1:2669656/139‑1 (MQ=14)

                 ccgggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                      <  1:3939354/137‑1 (MQ=255)

                 accggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAgg                                                                                                                      <  2:3382344‑M1/29‑1 (MQ=255)

                 aCTGGGGAAGCCGGACCCGCCGGAGCGACCGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                      <  1:1683957/140‑1 (MQ=255)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACCGGAGCGACC‑GGGCCGCCGGCGCAGCCGGAGCGACAGGa                                                                                                                     >  2:1643770/1‑140 (MQ=14)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGAGCGACCGGAGCGCCCGGGGCAGCCGGACAAACCGGAGCgcccgg                                                                                                                      >  2:609040/1‑135 (MQ=11)

                 aCTGGGGAAGCCGGACCAGCCGGAGCGACACGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGTCCGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                      <  1:3939355/140‑1 (MQ=255)

                 aCTGGCGAAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                      <  1:936746/140‑1 (MQ=14)

                      agcgacgggagccacaggggctcctggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccaccggggctcctGGGCCCGGCGGACCAGCCGGAgcgccagccgcga                                                                                                                 <  2:2311011‑M1/34‑7 (MQ=255)

                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGAGACGCAGGAACAACCGCAGCGACATGAGCTACAGGGGACTCAGGACCAGCCGGAGCGCCAGGAGCTAc                                                                                                                >  1:2773157/1‑140 (MQ=14)

                          gCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGAGCAGCCTGAGCGACATCAGCTACCGGGCAAGCCGGACCAACCGGTGCGAGAGGAGCTACCGCGGAAGCAGGACCAGCCGGGGCGACAGGAGCTACCgg                                                                                                             >  1:3485701/1‑140 (MQ=14)

                           cgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgacgggagccaccggggcgcctGGGGACGGCGGACCAGCCGGAGCGCCAGCAGCTCCCggg                                                                                                            <  2:3192327‑M1/39‑1 (MQ=255)

                           ccGGACCCGCAGGAGCGAGTTGAGCTACCGGGGTTGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                            <  1:3340074/140‑1 (MQ=14)

                                    ccGGCGCGACAGGAGCTACCGGCGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                   <  1:2505022/140‑1 (MQ=255)

                                    ccGGAGCGCCTGGAGCCTCGGGGGACCCCCGACCAGACGGAGCCACAGGAGCTCACGGTGAACCCGGACCAACCGGAGCCACAGGAGCTACAGGCCAAGCGGGACCAGCGGGAGCCACAGGAGCTACCGGGGAAGCCGGa                                                                                                   <  1:1799264/140‑1 (MQ=9)

                                    ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                   >  1:982882/1‑140 (MQ=255)

                                        aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAGGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                                               <  1:1235956/140‑1 (MQ=255)

                                             cAGGAGCGCCCGGGGACGCCGGACCCGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                          <  1:3874389/140‑1 (MQ=14)

                                             cAGGAGCGACCGGGGACGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                          <  1:774268/140‑1 (MQ=255)

                                                   cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGTAGCGACAGGGGACGACGGACCAACCGGAGCCACg                                                                                    >  1:113854/1‑140 (MQ=14)

                                                     aCCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                  <  2:768044/140‑1 (MQ=255)

                                                     aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                  <  2:349043/140‑1 (MQ=255)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAACAGGAACAGCCGGAGCGACAGTAGCAACCGACGAATCCAGACCACACGCAGAGACAGGac                                                                                >  1:1026467/1‑139 (MQ=11)

                                                       cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCAGGACCAGCCGGAGCGACCGGAGCCACCGGGGCAGCCCGAGCAGGCGGAGCGACAGGAGCGACAGGGGACGCCGGACCCACCGGAGCGACCGGAg                                                                                >  2:1902338/1‑140 (MQ=11)

                                                         ggcaaGCTGGACCACCGGGAGCCCCAGGAGATACGGGCGACCCCGGACCTACCGGCGACCCAGGACCTCCCGGGGATTCCGGACCCCCCGGAGCTACAGGTGCTCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                                              <  2:966804/137‑1 (MQ=2)

                                                           gAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            <  1:3952849/140‑1 (MQ=255)

                                                            caGCCGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAGCCGGGGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                           <  2:1586292/139‑1 (MQ=14)

                                                            aaGCCGGACCCGCCGGCGCCAGAGGCGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                           <  2:390298/140‑1 (MQ=14)

                                                             aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCCGCCGGACCAACAGGAGCGACAGGAGCGACCGGGGACGCCGTACCAGACTGAGCGTCAGGAGCTCGCGCGCAAGCCGGTCCACCCGGAGCGGCGGGAGCCCCAg                                                                          >  1:1960971/1‑140 (MQ=14)

                                                             aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACTCCGGACCAGCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                                                          >  1:2962203/1‑140 (MQ=255)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCCGGCCCAGCCGCAGCGACAGGAGCGCCCGGCGCCGCCGGACAAGCGGGTGCGCCGGGCGCGACAgg                                                                         >  2:170225/1‑140 (MQ=11)

                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                         <  1:1041743/140‑1 (MQ=255)

                                                              cccGGACCCCCCGGAGCGCCAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCCCCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAgg                                                                         <  1:1431403/139‑1 (MQ=14)

                                                                cgcaacAGCCGGAGCGACAGGCGCTACCGGTGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  2:248733/135‑1 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACTCCGGACCAGCCGGAGCGACTGGAGCAACCGGGGCAGCCGGACCAACCGGAGCGACGGGATCCACAggag                                                                       >  2:1998197/1‑138 (MQ=14)

                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCACCCGGAGCGCCGGGAGCCACAggcg                                                                       >  2:3943661/1‑138 (MQ=255)

                                                                cGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                       <  1:2186345/140‑1 (MQ=255)

                                                                            cgctcccggAGCTACCGGGGCATGAGGACCAACCGGAGCCACAGGAGCTACGGGTGAAGCCGGAGCAGCGGGAGCGTCAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                           <  1:3673718/133‑1 (MQ=17)

                                                                            agccccAGGAGCTCCCGGTGAACCCGGAGCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                           <  1:2751041/135‑1 (MQ=17)

                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGTGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                           >  2:2892494/1‑140 (MQ=255)

                                                                            aGCCACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                           <  1:3707886/140‑1 (MQ=255)

                                                                              ctaccggggaagctggaccagctggagcgacgggagcgACAGGAGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGGGCTACTGGGGACGTc                                                         >  1:72225‑M2/39‑140 (MQ=255)

                                                                                 ccggggacgccggacccaccggagccacgggagccaccggcgcttctGGCGACGCCGGAGCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                      >  1:1738283‑M1/48‑140 (MQ=255)

                                                                                  aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                                    <  2:1741196/93‑1 (MQ=37)

                                                                                      gacgccggcccagccggagccaccgccgccACAGCCGCTACCGGGGACGGCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                                 <  2:1868117‑M2/110‑1 (MQ=255)

                                                                                       cgtccGGGGCCGCCGGTCCAACCGGTGCTACAGGTGCTTCCGGGGAAGCGGGACCCCCGGGAGCGACAGGAGCTAGCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                                                <  2:737555/137‑1 (MQ=18)

                                                                                         gccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACcagcggg                                              <  1:518771‑M1/101‑4 (MQ=255)

                                                                                         gccggaccaaccggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                              <  1:518341‑M1/101‑1 (MQ=255)

                                                                                         actggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGCCCAGCCGGAGCGACGGGAGCCACCGGGGCGACGGGGGACGCCGGAGCCGCCgg                                              >  2:656548/4‑140 (MQ=11)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            >  2:2675495/1‑110 (MQ=255)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                              >  2:2250113/1‑140 (MQ=255)

                                                                                         aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                              >  2:2227877/1‑140 (MQ=255)

                                                                                          ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCTGCAGGAGCGACAGGGGAAGCCGGACCAACCGGAGCCTCGGGAGCCACAGGGGCTACTGGGGAGCTCGGACCAGCCGGa                                             >  2:1424556/1‑140 (MQ=18)

                                                                                           cgcaccagcgggagcgacgggagcgACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                            <  2:72225‑M2/115‑1 (MQ=255)

                                                                                            ggggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                  >  2:3924948/1‑101 (MQ=35)

                                                                                            ggggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                  <  1:1926187/101‑1 (MQ=35)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCAACCGGCGACGCCGGCCCACCCGGAGCGCCGGGAGCCACAGCCGCGACGGGGGACGTCGGACCAGCCGGAGCgcc                                        >  2:3132731/1‑138 (MQ=17)

                                                                                               gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                                 >  2:1677780/1‑51 (MQ=35)

                                                                                                  gCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGAACAACCGGAGCTAGGGTAGCCACAGGGGGTACGGGGGACCTCGGACCGGCCTGAGCGACAgg                                     >  2:803670/1‑140 (MQ=17)

                                                                                                  accggagcgacgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGTAGCGACAgg                                     <  2:2932203‑M1/110‑1 (MQ=255)

                                                                                                   ccggagcgaccggagcgaCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                            <  1:1610076/68‑1 (MQ=255)

                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                  <  2:3842468/38‑1 (MQ=33)

                                                                                                           aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                            >  2:2266564/1‑140 (MQ=255)

                                                                                                            cgggagccacaggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                           <  1:3105407‑M1/120‑1 (MQ=255)

                                                                                                                cgCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGCCGGGAGCCACAGGGGCTCCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                       <  1:1932502/139‑1 (MQ=255)

                                                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAACCAGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                       >  1:97546/1‑140 (MQ=255)

                                                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGCAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                       >  1:1176545/1‑140 (MQ=255)

                                                                                                                  ccacaggggctactGGGGACGCCGGACCAGCCGGAGCGACAgg                                                                                                                      <  1:2176340‑M1/29‑1 (MQ=255)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGCAGCGCCGGGAGCCCCAGGGGCGACTGGGGCCGTCGGACCTGCCGGAGGGACCCGAGCGACCGGGGACGCggg                   >  2:1244850/1‑140 (MQ=18)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                   >  2:2128961/1‑140 (MQ=255)

                                                                                                                    aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                   >  2:1341913/1‑140 (MQ=255)

                                                                                                                      aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACCGGCGCTACCGGGGAAGCCGGAc                 >  2:2656426/1‑140 (MQ=255)

                                                                                                                       ggggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                <  1:3828165‑M1/131‑1 (MQ=255)

                                                                                                                         ggctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa              <  1:1218284‑M1/133‑1 (MQ=255)

                                                                                                                          gctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc             <  2:3425615‑M1/134‑1 (MQ=255)

                                                                                                                          gctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAGCCGGACCAAc             <  2:3667719‑M1/134‑1 (MQ=255)

                                                                                                                          gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc             >  1:1408861/1‑140 (MQ=255)

                                                                                                                          gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCACCTGGGGACTGCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAAc             >  1:225545/1‑140 (MQ=18)

                                                                                                                           ctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGCACCAGCCGTAGCGACAGGAGCTACCGGGGAAGCCTTACCAaca            <  2:694882‑M1/135‑2 (MQ=255)

                                                                                                                           ctactGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGCACCAGCCGGAGCGCCAGGCGCTACCGGGGAAGCCGGACCAAcc            <  2:482279‑M1/135‑1 (MQ=255)

                                                                                                                             actGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAACCGGAGCGACGGGCGCCACAGGGGCGACTGGGGACGTCGGACCCGCCGGAGCGACCGGAGCTACCGGGGAAgccggaccctccgg          <  2:2422172‑M1/137‑7 (MQ=255)

                                                                                                                             actGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          <  2:1928080‑M1/137‑1 (MQ=255)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGCGACGTCGGACCAGCGGGAGCGACAGCGGCTACCGTGGAATCCTTACCAACggg          >  2:928253/1‑140 (MQ=18)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCAACAGGGGAAGTCGGACCAGCCGTAGCGCCAGGAGCTACCGGGGAATCCTGACCaccacg          >  2:1418899/1‑135 (MQ=17)

                                                                                                                             aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg          >  2:3346498/1‑140 (MQ=255)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                            >  1:339819/1‑68 (MQ=40)

                                                                                                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa         >  1:3924499/1‑135 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcgg  >  2:1390615/1‑138 (MQ=255)

                                                                                                                                     aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcgg  >  2:2427533/1‑138 (MQ=255)

CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAG  >  NZ_CP034943/607567‑607839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`