New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 607179 =239 (1.750)7 (0.050) 3/254 7.9 4.0% coding (22/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607466 114 (0.890)coding (309/1125 nt) EO946_RS03185 collagen‑like protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGTCGCTCCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/607325‑607179
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctcctgtCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCT  <  NZ_CP034943/607466‑607328
                                                                                                                                                                                                                                                                                              
TGTCGCTCCCGTCGCTCCGGCTGGTCCCGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAG                                                                                                                                                    <  2:46118/140‑1
TGTCGCTCCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAG                                                                                                                                                    <  2:1416553/140‑1
TGTCGCTCCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAG                                                                                                                                                    <  2:115945/140‑1
 GTCGCTCCCGTCGCTCCGGCTGGTCCCGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGC                                                                                                                                                   >  2:1339901/1‑140
 GTCGCTCCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCATGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGC                                                                                                                                                   >  2:273607/1‑140
     CTCCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGCTCCT                                                                                                                                               >  2:545054/1‑140
      TCCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTG                                                                                                                                              >  1:1101083/1‑140
       CCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGT                                                                                                                                             >  2:1280892/1‑140
       CCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGT                                                                                                                                             >  2:514516/1‑140
       CCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGT                                                                                                                                             >  2:1472309/1‑140
       CCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGT                                                                                                                                             >  1:778549/1‑140
       CCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAG                                                                                                                                                    >  2:1530006/1‑133
       CCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAG                                                                                                                                                    <  1:1530006/133‑1
       CCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCGTCCCCGGTAGCTCCTGT                                                                                                                                             >  2:400658/1‑140
                                                                                                                   CCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGT                                                                                                                  <  1:1657553/59‑1
                                                                                                                               GCTTCCCCGGTAGCTCCTGTCGCTCCGGCTGGTCCGGCTTC                                                                                                                        <  1:1345726/41‑1
                                                                                                                                CTTCCCCGGTAGCTCCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTG                                                                                                          >  2:1047016/1‑54
                                                                                                                                CTTCCCCGGGAGCTCCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTG                                                                                                          <  1:1047016/54‑1
                                                                                                                                    CCCGGTAGCTCCTGTCGCTCCGGCTGGTCCGGCTTC                                                                                                                        <  1:1385874/36‑1
                                                                                                                                         TAGCTCCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGT                                                                          <  1:30973/77‑1
                                                                                                                                         TAGCTCCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGT                                                                          >  2:30973/1‑77
                                                                                                                                           ggTCCTGTCTCTCCGGCTGGTGCGGCGTCCCCGGTAGCTCCTGTCGCTCCTGTCGGTCCGGTTTGTCCGGATTGTCCGGCTTCCCCGGTTGCTCCCGTTGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCC         <  1:1398104/138‑1
                                                                                                                                           ccgCCTGGCGCTCCCGCTGGTCCGGCTTCCCCGGTCGCACCTGTCGCTCCGGTCGCTCCGGTTGGTCCGGATGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCC         <  1:524309/137‑1
                                                                                                                                              CCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGT      <  1:1119676/140‑1
                                                                                                                                              CCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGT      <  1:1024542/140‑1
                                                                                                                                              CCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGT      <  2:1816324/140‑1
                                                                                                                                              CCTGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGT      >  2:199465/1‑140
                                                                                                                                                ccTCGCGCCGGCTGGTCCGGCTTCGCCGGTCGCTGCGCTCGCTCCTGTCGCTCCGGTTGGTCCGGCGGGTCCGGTTTCCCCGGTAGCTCCGGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAG    <  2:1347106/138‑1
                                                                                                                                                TGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAG    <  1:638199/140‑1
                                                                                                                                                TGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAG    >  2:1605784/1‑140
                                                                                                                                                TGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCCGGTGGTCCGGCTGGGCCGGCTTCGCCGGTAGC   >  2:1408509/1‑140
                                                                                                                                                 GTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAG    <  2:1754744/139‑1
                                                                                                                                                 GTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAG    >  1:2076708/1‑139
                                                                                                                                                 GTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAG    <  2:2076708/139‑1
                                                                                                                                                 GTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAG    >  1:1754744/1‑139
                                                                                                                                                 GTCGCTCCGCCCGCTCCGGCCGCCCCGGTAGCTCCTGACGCTCCTGGCGCTCCGGTCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCGGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGC   <  2:159344/140‑1
                                                                                                                                                  TCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCT  >  1:1252349/1‑140
                                                                                                                                                                                                                                                                                              
TGTCGCTCCCGTCGCTCCGGCTGGTCCAGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGCTGGTCCGGCTGGTCCAGCTTCCCCAGTAGCTCCCGTGGCTCCTGTCGCCCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/607325‑607179
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctcctgtCGCTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCTGTCGCTCCTGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCTCCCGTGGCTCCCGTCGCTCCGGTTGGTCCGGCTGGTCCGGCTTCCCCGGTAGCT  <  NZ_CP034943/607466‑607328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.