New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP034943	= 2408000	91 (0.660)	3 (0.020)	3/270	8.2	3.3%	coding (493/621 nt)	spoIIIAF	stage III sporulation protein AF
	?	NZ_CP034943	2408004 =	87 (0.640)					coding (489/621 nt)	spoIIIAF	stage III sporulation protein AF
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCCGTTTTTATTCATTGCCGACACTCTCCCCGCCTTCCATATGAACCGTAATTTTCTCACTTCCGATTTCCCAAATGTCCGCAAGCTGTAACTGGATCTGTTTGGCTTCTTTTTGTTCGGCTGCTTCTTTCGTTAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/2407865‑2408000 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGCATGATCTGTATCAATGTGGACCGGCGCGACCGTTTGGACCGTTTTTTCAGAAGACGGGGCCATATACACGCTGATTGTTTTAATATC  >  NZ_CP034943/2408004‑2408093                                                                                                                                                                                                                                    TCCGTTTTTATTCATTGCCGACACTCTCCCCGCCTTCCATATGAACCGTAATTTTCTCACTTCCGATTTCCCAAATGTACGCAAGCTGTAACTGGATCTGTTTGGCTTCTTTTTGTTCGGCTGCTTCTTTCGTTACTtat                                                                                        >  1:401384/1‑137                                               CGTAATTTTCTCACTTCCGATTTCCCAAATGTCCGCAAGCTGTAACTGGATCTGTTTGGCTTCTTTTTGTTCGGCTGCTTCTTTCGTTACTGCATGATCTGTATCAATGTGGACCGGCGCGACCGTTTGGACCGTTTTTT                                          >  2:2049294/1‑140                                                                                       TGTAACTGGATCTGTTTGGCTTCTTTTTGTTCGGCTGCTTCTTTCGTTACTGCATGATCTGTATCAATGTGGACCGGCGCGACCGTTTGGACCGTTTTTTCAGAAGACGGGGCCATATACACGCTGATTGTTTTAATATC  <  1:2049294/140‑1                                                                                                                                                                                                                                    TCCGTTTTTATTCATTGCCGACACTCTCCCCGCCTTCCATATGAACCGTAATTTTCTCACTTCCGATTTCCCAAATGTCCGCAAGCTGTAACTGGATCTGTTTGGCTTCTTTTTGTTCGGCTGCTTCTTTCGTTAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/2407865‑2408000 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGCATGATCTGTATCAATGTGGACCGGCGCGACCGTTTGGACCGTTTTTTCAGAAGACGGGGCCATATACACGCTGATTGTTTTAATATC  >  NZ_CP034943/2408004‑2408093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.