Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943514,9680GT25.6% 182.1 / 21.8 90V128L (GTA→TTA) EO946_RS02645outer membrane lipoprotein carrier protein LolA
Reads supporting (aligned to +/- strand):  ref base G (51/16);  new base T (1/22);  total (52/38)
Fisher's exact test for biased strand distribution p-value = 7.44e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGAAAAAGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAG  >  NZ_CP034943/514830‑515104
                                                                                                                                           |                                                                                                                                        
ccGAAAAAGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGt                                                                                                                                         <  1:1340061/140‑1 (MQ=255)
ccGAAAAAGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGt                                                                                                                                         <  1:1666459/140‑1 (MQ=255)
     aaaGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAgg                                                                                                                                    <  1:1057960/140‑1 (MQ=255)
     aaaGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAgg                                                                                                                                    <  1:76883/140‑1 (MQ=255)
     aaaGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAgg                                                                                                                                    <  1:1786338/140‑1 (MQ=255)
                cacAAGTAATTTTACTAAATCAAAACGTCTTATTTGTTATCACGCCGTCTTTAAATAACTGATTCAGTTTTAAACGCTACTGGCCGAACAACAGCAGCCTGGACTACTTTTTTTAATCGCTCTTAACGGTTGTAAAAAAc                                                                                                                         <  2:1340390/138‑1 (MQ=255)
                   aaGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGAc                                                                                                                      >  1:711877/1‑140 (MQ=255)
                    aGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACt                                                                                                                     >  2:310238/1‑140 (MQ=255)
                         ttttACGAAATGAAACGGGCTTTTTTGTTCTCACGCGGTCTTTAAATAAGTGGTTCTGGTTTAAACGCGAGGGGCCCAACAACAGCAGCCAGGTCTAGTTGTTTGAATCGCTCGTAACGGATGTTAAAAACGACTCGGAt                                                                                                                <  2:700800/140‑1 (MQ=255)
                          tttACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATg                                                                                                               >  1:342480/1‑140 (MQ=255)
                          tttACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATg                                                                                                               >  2:632729/1‑140 (MQ=255)
                          tttACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATg                                                                                                               >  2:1891513/1‑140 (MQ=255)
                                      aaaCGGTTTTTTGGTTCTCACGCCTTTTTTAAATAAGAGCTTCTGTTTACAACGCGACGGGCCCAACACCCGCCGCCCGGTCTAGTTTTTTTAATCGCTCTTAAAGTATGTAAAACACGACTCGGATGCAGTTTTCACTg                                                                                                   <  1:1859465/140‑1 (MQ=255)
                                         cgtattATTTGTTCTCACGCCTTATTTAAATAAGAGCTTCAGGTTTCAACGCGACGGGCCGAACAACAGCAGCCAGGTCTAGTTTTTTGAATCGCTCTTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCa                                                                                                <  2:1104025/135‑1 (MQ=255)
                                            tttttttGTTCTCTCGCTTTTTTTAAATATGTGCTTCTGTTTTAAAAGCGAGTGGCCCAAAAACAGAAGCCTTGTTTATTTTTTTGAATCGCTCTAAAAGTTTGTAAAACACGACTCGGATGCAGTTTTCACTGCCaaag                                                                                             <  1:1737482/136‑1 (MQ=255)
                                              tttttGTTATCACGCCGTATTTAAATAAGAGCTTCAGTTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTATACTTGTTTGAATCGCTCTTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCaaagaa                                                                                           <  2:2066253/138‑1 (MQ=255)
                                              tATTTGTTCTCACGCCGTATTTAAATAAGAGCGACAGGTATCAACGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTTTTTTAATCGCTCGTAAAGGATGTTCAACACGACTCGGATGCAGTTTTCACTGCCaaagaa                                                                                           <  2:1312620/140‑1 (MQ=255)
                                                  tgctctcACGCCTTATTTAAAACAGAGCTTCTGTTTCAAACGCGACGGCCCCAACAACAGCAGCCAGGTATACTTTTTTTAAGCGCTCTTAAAGGAGTTTCAAAACGACTCGGATGCAGTTTTCACTGCCaaagaaaaga                                                                                       <  1:1182053/137‑1 (MQ=255)
                                                    ttCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAagaaaagaaa                                                                                     >  1:2032530/1‑140 (MQ=255)
                                                      ctcACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGACAAGAAATa                                                                                   >  2:308130/1‑140 (MQ=255)
                                                      ctcACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATa                                                                                   >  1:234147/1‑140 (MQ=255)
                                                      ctcACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGGAATGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATa                                                                                   >  1:175601/1‑140 (MQ=255)
                                                       ccACGCGGTTTTTAAATAAGAGTTTCTGGTTACAACGCGACGGCCCCAAAAACAGCACCCAGTTTTACTTTTTTTAATCGCTCGTAAAGGTTTTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATAc                                                                                  <  1:43942/139‑1 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAACAGAAATACg                                                                                 >  2:2001467/1‑140 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACg                                                                                 >  1:770142/1‑140 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACg                                                                                 >  2:722469/1‑140 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCAATGCCAAAGAAAAGAAATACg                                                                                 >  1:451786/1‑140 (MQ=255)
                                                        cACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGGAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACg                                                                                 >  1:1206265/1‑140 (MQ=255)
                                                                       tAATAGCTTCAGTTTTAAAAGCGACTGGCCGAACAACAGAAGCCTGGTCTAATTTTTTGAATCGCTCGTAAAGGATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTaaaa                                                                  <  2:644060/140‑1 (MQ=255)
                                                                       tAAGACCTTATGGTTTAAAAGCGACTGGCCGAAAAACAGCAGCATGGTCTACTTGTTTTAATCGCTCTTAAAGTATTTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTaaaa                                                                  <  2:643502/140‑1 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCTTAAAGGATGTTCAACACGACTCGGATGCAGTTTTCACTGCCAAAGACAAGAAATACGTATTCGAAACTAAAACg                                                                >  2:526820/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                >  2:1415441/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                >  2:753476/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                >  2:216767/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                >  2:542225/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                >  2:567645/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                >  1:1606986/1‑140 (MQ=255)
                                                                         agagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACg                                                                >  2:753006/1‑140 (MQ=255)
                                                                          gagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGa                                                               >  1:561238/1‑140 (MQ=255)
                                                                          gagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGa                                                               >  1:984831/1‑140 (MQ=255)
                                                                          gagCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGa                                                               >  1:984863/1‑140 (MQ=255)
                                                                           agCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGaa                                                              >  1:188979/1‑140 (MQ=255)
                                                                           agCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGAAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGAGGTTCCAAACGACTCGGATGCGGTTTTCACTGCCAACGAAAAGAAATACGTATTCGACACTAAAACGaa                                                              >  1:302704/1‑140 (MQ=255)
                                                                           agCTTCAGGTTTCAAACCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGAGTCGGATGCAGTTTTCACTGACAAAGAAAAGAAATACGTCTTCGAAACTCAAACGac                                                              >  1:1188434/1‑139 (MQ=255)
                                                                               tcttttttCAAAGCGACTGGCCTAACAACAGCAGCCATGTCTATTTTTTTTAATCGATCTTAATGTATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                          <  2:1607922/135‑1 (MQ=255)
                                                                               tctgttttAAAAGCGACTGGCCTAACAACAGCAGCCTGTTATAGTTTTTTTAATGGATCTTAAAGTATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                          <  1:1239044/135‑1 (MQ=255)
                                                                               tctggTTTCAACGCGAGTGGCCGAACAACAGCAGCCAGTTATAGTTGTTTGAATCGATATTAAAGTATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                          <  1:1038837/137‑1 (MQ=255)
                                                                               tctggTTACAAAGCGACTGGCCTAACAACAGCTGCCAGGTATACTTTTTTGAATCGATCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                          <  2:2064866/137‑1 (MQ=255)
                                                                               taaGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                          <  2:622635/138‑1 (MQ=255)
                                                                               tCAGGTTTCAAAGCCACTGGCCGAACAACAGAAGCCAGGTCTATTTTTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTAt                                                          <  2:2015658/140‑1 (MQ=255)
                                                                                  ggTTTCAACGCGACTGGCCTAACAACCGCAGCCTGTTATACTTTTTTGAATCGCTCTTAACGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAg                                                       <  1:481104/140‑1 (MQ=255)
                                                                                   ttttCACAGGGAGGGGCCGAAAAACCGCCGCCTGTTCTATTTTTTTGAATGGCTTTTAAAGTTTTTTAAACACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGc                                                      <  1:310238/139‑1 (MQ=255)
                                                                                    tttCAACGCGAGGGGCCTAACAACAGCCGCCTGTTCTATTTTTTTGAATCGCTCTTAACGGTTTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCa                                                     <  1:589546/140‑1 (MQ=255)
                                                                                    tttCAAAGGGACTGGCCTACAATCAGAAGCAATGTCTACTTTTTTGATTCGTTCTTAATGTTTTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCa                                                     <  2:111967/140‑1 (MQ=255)
                                                                                    tttCAAAGGGACTGGCCCAACAACAGCAGCCTGTTCTTCTTTTTTTAATCGCTCTTAATGTATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCa                                                     <  1:1331372/140‑1 (MQ=255)
                                                                                       aaaacgCGACTGCCCTAAAAACAGCACCCTGTTATACTTTTTTTAATCGCTCGTAAAGCATGTAAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATa                                                   <  1:1789573/135‑1 (MQ=255)
                                                                                                cTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAAtgc                                          >  2:625425/1‑140 (MQ=255)
                                                                                                cTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAAtgc                                          >  2:1672752/1‑140 (MQ=255)
                                                                                                      caacaaaAGCACCCTGGTCTACTTGTTTGAATCGCTCGTAAAGGATTTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAACCGAACTATCAGCATAACAAAATGCTGCCaa                                    <  1:1205492/139‑1 (MQ=255)
                                                                                                         aaacaGCAGCCAGTTATTCTTTTTTTAATCGCTCTTAAAGTTTGTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGc                                 <  1:860360/139‑1 (MQ=255)
                                                                                                             agcagcCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGa                             >  2:244280/1‑140 (MQ=255)
                                                                                                              gcagcCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAAAGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTACAACGAACTATCAGCATAACAAAATGCTGACAAAGCAAGaa                            >  2:149132/1‑140 (MQ=255)
                                                                                                                  ctaGGTCTACTTGTTTGAATCGCTCGGAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACCCAAGAAATCa                        >  2:401403/3‑140 (MQ=255)
                                                                                                                      gTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTTCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGtt                    >  1:438351/1‑140 (MQ=255)
                                                                                                                      gTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCACGAAATCACGtt                    >  2:78792/1‑140 (MQ=255)
                                                                                                                           cTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACa               >  2:1023227/1‑140 (MQ=255)
                                                                                                                           cTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCACCATAACCAAATGCTGCCAACGCAAGACATCACGTTTAAcc               >  2:1116175/1‑139 (MQ=255)
                                                                                                                            ttGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaa              >  1:427492/1‑140 (MQ=255)
                                                                                                                             ttttttAATCGCTGTTAAAGGATGTAAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaa             <  2:1893133/138‑1 (MQ=255)
                                                                                                                              tttttAATCGCTCTTAACGTATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaa            <  2:188979/139‑1 (MQ=255)
                                                                                                                              tttttAATCGCTCTTAAAGTATGTTAAACACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaa            <  1:1177307/139‑1 (MQ=255)
                                                                                                                              tttttAATCGATCTTAAAGGATTTACAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATACCAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaa            <  1:454514/139‑1 (MQ=255)
                                                                                                                              ttttGAATCGCTCGTAACGGATTTACAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaa            <  2:1413343/139‑1 (MQ=255)
                                                                                                                              ttttGAATCGCTCGTAAAGGTTTTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaa            <  2:366323/139‑1 (MQ=255)
                                                                                                                               ttttAATCGCTCTTAAAGGATTTTAAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaaa           <  1:892613/140‑1 (MQ=255)
                                                                                                                               ttttAATCGATCTAAAAGCATTTTAAACACGACTCGGATTCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACaaaaa           <  1:1514210/140‑1 (MQ=255)
                                                                                                                                 tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGTAATCACGTTTAACAAAAAAg         >  1:541277/1‑140 (MQ=255)
                                                                                                                                 tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg         >  1:1012659/1‑140 (MQ=255)
                                                                                                                                 tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg         >  2:1401896/1‑140 (MQ=255)
                                                                                                                                 tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg         >  1:997009/1‑140 (MQ=255)
                                                                                                                                 tGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAg         >  1:1893133/1‑140 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGAGTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCCTAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGatatcac  >  2:1688351/1‑137 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCCAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAg  >  2:59356/1‑140 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGACATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAg  >  2:1937473/1‑140 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATgcg  >  1:478159/1‑138 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAg  >  2:1424245/1‑140 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAg  >  2:349135/1‑140 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAg  >  2:1118737/1‑140 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAAAAAAAAAGATATGAg  >  2:1470500/1‑140 (MQ=255)
                                                                                                                                        cTCGTAAAGGATGTTCAAAACGACTCGGAAGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAAACACGTTTAACAAAAAAGATATGAg  >  2:1470395/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CCGAAAAAGGACCAAAATCAAGTCATTTTACGAAATGAAAACGGAGTATTTGTTCTCACGCCGTCTTTAAATAAGAGCTTCAGGTTTCAAAGCGACTGGCCGAACAACAGCAGCCAGGTCTACTTGTTTGAATCGCTCGTAAAGGATGTTCAAAACGACTCGGATGCAGTTTTCACTGCCAAAGAAAAGAAATACGTATTCGAAACTAAAACGAACTATCAGCATAACAAAATGCTGCCAACGCAAGAAATCACGTTTAACAAAAAAGATATGAG  >  NZ_CP034943/514830‑515104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: