Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2270TG35.9% 77.3 / 32.1 78intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base T (15/35);  new base G (28/0);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.77e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363
                                                                                                                                        |                                                                                                                                               
ataCGAGCCACTGGAAAGCTCTAAAGCGTACTATGATTGGGTGACACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAATGTAATACAAAAACGCCTGGGAAACCGGGCGtgttt                                                                                                                                              >  2:349433/1‑140 (MQ=255)
ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAACCCGGGCGtgttt                                                                                                                                              >  2:221993/1‑140 (MQ=255)
ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCgttgtt                                                                                                                                              >  1:1078747/1‑140 (MQ=255)
ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtgttt                                                                                                                                              >  1:233774/1‑140 (MQ=255)
ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtgttt                                                                                                                                              >  1:1076297/1‑140 (MQ=255)
ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGtgttt                                                                                                                                              >  1:1593106/1‑140 (MQ=255)
   cGAGCAACTGGAAAGCTCTATAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTg                                                                                                                                           >  2:1041476/1‑140 (MQ=255)
   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACGGATTGAAACAAAGTCAAACAAAAACGCCTGGGAAACCGTGCGttgtttgg                                                                                                                                           >  2:2027942/1‑138 (MQ=255)
   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTg                                                                                                                                           >  1:1120924/1‑140 (MQ=255)
     accAACTGCAAACCTCTAACGCTAACTAGGATGGGCTCAAACGGCTTGCTGAAGCGGCGCCCAGCGGGGAAATTTTTAAATTTAAACCTATTGAAAAAAAGTAAAAAAAACACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                         <  1:854909/138‑1 (MQ=255)
     aGCACCTGGAAAGCTCTAAAGCTTACTATGATGGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                         >  2:542120/1‑140 (MQ=255)
     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTTAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAAAGCATGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                         >  1:854780/1‑140 (MQ=255)
     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                         >  1:1252353/1‑140 (MQ=255)
     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                         >  2:542140/1‑140 (MQ=255)
     aGCAACTGGAAAGCTCTAAAGCTTACTATGAGTGGCTGAAACGGATTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATGTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                         >  2:751561/1‑140 (MQ=255)
      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTACAACACAAACGCCGGGGCAACCGGGCGTGTTTTTGttt                                                                                                                                        >  2:1229905/1‑139 (MQ=255)
      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTGTTTTTGttg                                                                                                                                        >  2:1230469/1‑139 (MQ=255)
      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGttttttttttg                                                                                                                                        >  2:953129/1‑139 (MQ=255)
       cAACTGGAAAGCTCTAAAGCTTAATATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGCGCGGGGAAATTTTTAAATTAATACCTATTGAAACAAAGTAACAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCt                                                                                                                                      <  1:2036633/140‑1 (MQ=255)
        aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTGTTCtt                                                                                                                                     >  2:1730686/1‑140 (MQ=255)
         aCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCGTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTAttt                                                                                                                                    >  2:182435/1‑140 (MQ=255)
          cTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGtttt                                                                                                                                   >  2:1525765/1‑140 (MQ=255)
          cTGCAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGACTCGGTGCCGCGCGGGTAATTTTTTAAATTTATACCGATTGAAACAAAGAAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCtttt                                                                                                                                   <  1:221993/140‑1 (MQ=255)
                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGGTTTTTTTTTaa                                                                                                                           >  1:1162549/1‑140 (MQ=255)
                    ctAAAGCTTACTATGATTGGCTGAAAAGGCTTGCTGAATCGGTGCATAGCGGGGAAATTTTTAAATTTATACCGATTGAAACACAGTAAAACAAAAACGCCTGGGACACCGGGCGTTTTTTTTGTGTTTTTGATGTacat                                                                                                                         >  1:61596/1‑137 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTAGTTTTTATGTAATTAAAACaaa                                                                                                              >  2:1258925/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTCTTTTTTATTTTATtaataagaa                                                                                                               >  1:718298/1‑136 (MQ=255)
                             aCTATGATTGGCTGAACCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATtactaaacaa                                                                                                              >  1:925963/1‑135 (MQ=255)
                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTAATTAATAaaaaa                                                                                                             >  1:792993/1‑139 (MQ=255)
                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTTATTAATACAAAt                                                                                                            >  1:793088/1‑140 (MQ=255)
                                aTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTg                                                                                                         <  1:1444966/140‑1 (MQ=255)
                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTAATAAAAATTTTaaa                                                                                                      >  2:2032815/1‑140 (MQ=255)
                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAAATACTACAAATTTGaaaa                                                                                                     >  2:1444966/1‑140 (MQ=255)
                                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTTATTACTACAAATTTTGaaaaaa                                                                                                   >  2:146222/1‑139 (MQ=255)
                                             aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTAATTTATAAAAATTTTTAAAATAAGGGaa                                                                                           >  1:672455/1‑140 (MQ=255)
                                                         aTCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCAtt                                                                              <  1:953083/140‑1 (MQ=255)
                                                          tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTCTTTTTTATTTAATTAATAAAAATTTTAAAAATAAGGGAAAAAAATACCAttt                                                                             >  2:651579/1‑140 (MQ=255)
                                                               gCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTATTTTATTACTACAAAATTGTAAAATAACGGTAAAAACAACCCAttacttg                                                                         >  1:326265/1‑135 (MQ=255)
                                                                  gagCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTACTACAAATTTTAAAAAAACGGGGACAAGATCACATTTACTTACaa                                                                     >  2:1828420/1‑140 (MQ=255)
                                                                     cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTGGTTGTTTTTTTTGTAATTACGACAAATTTTAAAAATAACGGACAAACATCCCAATTACTTCCAAAat                                                                  >  2:388384/1‑140 (MQ=255)
                                                                     cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAATTAATAAAAAATTTAAAAATAAGGGAAAAAAATACCCATTTATTGCaaaag                                                                  >  2:832878/1‑139 (MQ=255)
                                                                       gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTTCTACAAATTTGAAAAATAAGGGTAATACATAGCCTTTTCTTACAAAatat                                                                >  2:1464673/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTAATTACTAAAAATTTTAAAAATAAGGGAAAAAAATACCATTTAAGTACAAAATAtt                                                               >  2:926808/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTGTTGTTCTTTTTTATGTAATTTATACAAATTTTAAAAATAAGGGTAAAACATCCCCTTTACTTGCAAAAGAtt                                                               >  2:1516590/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTTTTGTTTTTTATTTAATTAATAAAAATTTGAAAAATTAGGGAAAAAAAAGCCATTTACTTCCAAAATAtt                                                               >  2:380499/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTTTTTAATTAATAAAAATTTGAAAAATACCGGGAAAAAATACCATTTTCTTCCAAAATAtt                                                               >  2:1557705/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTAATTAAGAAAAAATTTTAAAAAAACGGAAAAAAAAACCATATAATTGCAAAATAtt                                                               >  1:451638/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGGTTTTTTTTGTAATACTAAAAAATTGTAAAATAAGGGAAAAAAAAACCATTTAATTCGCAAATAtt                                                               >  1:1959390/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAATTTATACAAAATTTTAAAATAAGGGAAAAACATACCCTTTAGTTCCAAAAGAtt                                                               >  2:964039/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAAATAATACAAATTTTAAAAATACGGGACAAACAAGCCAGATACGTCCAAAAAAtt                                                               >  2:336944/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGGTTTTTTTGTAATTACTAAACATTTTAAAAATAAGGGGAAAACATTCCAATTAAGTCCAAAATAtt                                                               >  2:43474/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTGTTTTTTTTTTATTGTATTTCTAGCAATTTTATAAAATAGGGCAAATACTCCCAGTTTCTTCGaagctatg                                                               >  2:1819246/1‑134 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTTGTTTTTTTGTTATTACTAAAAAATTTTAAAAAAACGGAAAAACAAACCAAGTAATTACaaaaaaat                                                               >  1:122502/1‑136 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTTGTTTTTTTTTTATTTATAAAAAATTTTAAAATAAGGGGAAAACATCCCAGTTTATTCCAAAAGAtt                                                               >  1:1564853/1‑140 (MQ=255)
                                                                              ttttAAATTTCGACCCATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  2:1342243/140‑1 (MQ=255)
                                                                              ttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  2:1991484/140‑1 (MQ=255)
                                                                                        ataCCTATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                               <  2:689970/140‑1 (MQ=255)
                                                                                             taTTCAACCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                          <  1:1682794/139‑1 (MQ=255)
                                                                                             gATTGAAACAAAGTAAAACAAAAACTCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                          <  2:1252353/140‑1 (MQ=255)
                                                                                                  aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:1781864/140‑1 (MQ=255)
                                                                                                        aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTAATTCCCAAATATTTGATGGTAATTTTATGTTGGTAAAATTTGTAt                               >  2:385771/1‑140 (MQ=255)
                                                                                                         gaaaaaCAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                              <  1:146222/138‑1 (MQ=255)
                                                                                                            aaccAAACACGCCTGGTAAACCGGTCTTTTATTTGTTCTTTATTAAGTAATTAATACAAATTTGAAAAATAAGTGAAAAACATCCCATTTACTTCCAAAATATTTGATTGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1240336/137‑1 (MQ=255)
                                                                                                            aaccAAACACGCCTGGGAAACCGGGCGTTTGTTTCTACTTTTTTATGTAATTACTACACATTTGAAAAATACGGGCAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:502355/137‑1 (MQ=255)
                                                                                                            aaacaaacACGCCTGGTAAACCGGGAGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGACAAATACGGGAAAAACATCCCATTTACTTCCAAACTATTGGATGGTACTTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1109576/140‑1 (MQ=255)
                                                                                                            aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:832878/140‑1 (MQ=255)
                                                                                                            aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1525765/140‑1 (MQ=255)
                                                                                                            aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:507430/140‑1 (MQ=255)
                                                                                                            aaaCAAAACCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:506852/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGTGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:152463/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1954814/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:2032815/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:793088/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:792993/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1593106/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:615446/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1076297/140‑1 (MQ=255)
                                                                                                            aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:233774/140‑1 (MQ=255)
                                                                                                                    aCGCCTGGGAAACCGGGCGTTTTTTTTTTTTTTTTTATGTAATTAATACAAAATTTAAAAATAAGGGGAAAAAAAACCAATTAATTACAAAATATTTGTTTGTAAATTTATTTATGTAAAATTTTTATTTTGGCGTTAag                   >  2:69547/1‑140 (MQ=255)
                                                                                                                      gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:892849/140‑1 (MQ=255)
                                                                                                                      gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:574867/140‑1 (MQ=255)
                                                                                                                                     cttttttttGTTATTTTTTATGTAATTACTACAAATTTGAAAAATAAGGGAAATACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  2:1226867/138‑1 (MQ=255)
                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:1230469/140‑1 (MQ=255)
                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:1229905/140‑1 (MQ=255)
                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:1730686/140‑1 (MQ=255)
                                                                                                                                     cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:953129/140‑1 (MQ=255)
                                                                                                                                     cGTTTTTTTGTTATTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  2:1734230/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                               
ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: