Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 3,247,065 C→T intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,247,0650CT99.2% 479.3 / ‑4.3 130intergenic (‑11/+110)bdbD/cadAdisulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (91/38);  total (92/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 130 total aligned reads displayed.

TCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGAC  >  NZ_CP034943/3246929‑3247198
                                                                                                                                        |                                                                                                                                     
tCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATc                                                                                                                                    >  1:1665816/1‑140 (MQ=255)
       aCCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaa                                                                                                                             >  1:939073/1‑140 (MQ=255)
       aCCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaa                                                                                                                             >  1:1165711/1‑140 (MQ=255)
       aCCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaa                                                                                                                             >  1:1166072/1‑140 (MQ=255)
          gCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaat                                                                                                                          >  1:1616110/1‑140 (MQ=255)
          gCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaat                                                                                                                          >  2:133569/1‑140 (MQ=255)
          gCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaat                                                                                                                          >  1:2094065/1‑140 (MQ=255)
          gCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaat                                                                                                                          >  1:1291406/1‑140 (MQ=255)
                   ccTTGTTGCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGACTACTGCTGTTTCTTTTTCACTCCGACACCTTATAGTTTTAAAATTAAAAAaag                                                                                                                 >  1:1903189/1‑140 (MQ=255)
                        ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                            >  1:85472/1‑140 (MQ=255)
                           cGTTTTTTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  1:1318417/140‑1 (MQ=255)
                           cGTTTTGTTATTAACGATGCCAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  1:1461164/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAATACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  2:1291878/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  1:1566485/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  1:551132/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  2:806911/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  2:969671/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  2:36478/140‑1 (MQ=255)
                           cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  1:1158582/140‑1 (MQ=255)
                            gTTTTGTTATTAAAGAGGACAATGACTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATc                                                                                                        <  2:633054/140‑1 (MQ=255)
                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         >  1:484327/1‑138 (MQ=255)
                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  2:484327/138‑1 (MQ=255)
                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                       >  1:739031/1‑140 (MQ=255)
                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                       >  2:1912091/1‑140 (MQ=255)
                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                       >  2:1925961/1‑140 (MQ=255)
                             ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                       >  2:283431/1‑140 (MQ=255)
                                    attaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                          >  2:35514/1‑130 (MQ=255)
                                    attaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                          <  1:35514/130‑1 (MQ=255)
                                      taAAGATGACAATGGCTGCTAGTAAGACAACACCCACAACCGTAAGTAGGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCACTAGAcc                                                                                              <  1:1951089/140‑1 (MQ=255)
                                      taAAGATGACAATGGCTGCTAGTAAGACAACACCCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                              <  1:352983/140‑1 (MQ=255)
                                                 aTGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAt                                                                                   <  1:986075/140‑1 (MQ=255)
                                                  tGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAtt                                                                                  >  1:1310752/1‑140 (MQ=255)
                                                    gctgctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAtttt                                                                                >  2:1132042/1‑140 (MQ=255)
                                                          aGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaag                                                                          >  2:622200/1‑140 (MQ=255)
                                                          aGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTAACTCCGACACCTTATCTTTTTAAAATTAAAAAAACAAGCTGATCGCAATAGACCCACTTATTTATTGTAacaat                                                                          >  2:1244964/1‑139 (MQ=255)
                                                            tAAGACCACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCCCTCCGACACCTTCTCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAaccagac                                                                        <  2:1966891/140‑1 (MQ=255)
                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                      <  2:477503/140‑1 (MQ=255)
                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                      >  2:1921479/1‑140 (MQ=255)
                                                              aGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTACTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagacaa                                                                      <  1:1255101/140‑1 (MQ=255)
                                                                acaacaACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGt                                                                    <  2:2079277/140‑1 (MQ=255)
                                                                  aacaacCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGc                                                                  >  1:449407/1‑140 (MQ=255)
                                                                   acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                                 >  1:1461934/1‑140 (MQ=255)
                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCAGACTACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTGCGTTATTTTAACAAGACAAGTGCCt                                                                >  1:476609/1‑140 (MQ=255)
                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTCTCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACACGTGCCt                                                                >  2:1248592/1‑140 (MQ=255)
                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCt                                                                >  2:1732269/1‑140 (MQ=255)
                                                                    caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACACACTTCTTTATTTTAACAAGACAAGTGCCt                                                                >  2:1426417/1‑140 (MQ=255)
                                                                     aaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCta                                                               <  2:263000/140‑1 (MQ=255)
                                                                     aaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCta                                                               <  2:262343/140‑1 (MQ=255)
                                                                      accacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCtat                                                              >  1:573079/1‑140 (MQ=255)
                                                                       ccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTGTAAAATTAAAAAAAGAAGCGGAGCGCAATAGACCCACTGCGTTATTTTAACAAGACAAGTGccacga                                                             >  2:1769656/1‑136 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAATACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                          >  1:1479119/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                          >  1:50654/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                          >  2:1295023/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                          >  2:1814385/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                          >  2:771810/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                          >  2:1814222/1‑140 (MQ=255)
                                                                          caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                          >  1:1418784/1‑140 (MQ=255)
                                                                               gTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAAcc                                                     >  2:635824/1‑140 (MQ=255)
                                                                                     aTGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaa                                               >  2:906084/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAATACTGCTGTGTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAACAAAACCCCGaaaaaa                                            >  1:180332/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  2:101567/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  1:188315/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  1:1481217/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  2:1591111/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  1:1589102/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  2:862495/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  1:302600/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  1:1276248/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  2:387577/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                            >  1:1128255/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTGTAAAAGTAAAAAAAGACGCGGATCGCAATAGACCCCCTTCTTTATGTTAACAAGACAAGTGCCTATAAGAACACCCCGacaact                                            >  1:1859665/1‑138 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTGTTAAAATTAAAAAAAGTAGCGGATCGCAATAGACCCACGTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGacacag                                            >  1:1595035/1‑135 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGGTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCGTTATTTTAACAAGACAAGTGCCTCTAAGAAAACCCCGaaaaaa                                            >  2:2006367/1‑140 (MQ=255)
                                                                                        aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAGAAGCGGATCGCAATTGACCCACTTCTTTATTTTAACAAGACAAGCGCTGATAAGAAAACCCCGAAAAAAt                                           >  2:1601171/1‑140 (MQ=255)
                                                                                          cgcgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         <  1:1987597/77‑1 (MQ=255)
                                                                                          cgcgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                         >  2:1987597/1‑77 (MQ=255)
                                                                                            cgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGaa                                        >  2:575002/1‑140 (MQ=255)
                                                                                              aaTTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAAtt                                      >  1:2012246/1‑140 (MQ=255)
                                                                                              aaTTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAAtt                                      >  1:564357/1‑140 (MQ=255)
                                                                                                          gaCTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACact                          >  1:1890403/1‑138 (MQ=255)
                                                                                                              gctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTAt                      <  1:1591111/140‑1 (MQ=255)
                                                                                                               ctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTcc                                >  2:994358/1‑129 (MQ=255)
                                                                                                               ctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATc                     <  1:961111/140‑1 (MQ=255)
                                                                                                               ctgTTTCTTTTTCACTCCGACACCGTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTGCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATc                     >  1:1961078/1‑140 (MQ=255)
                                                                                                                  tttCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTcc                                >  1:1215354/1‑126 (MQ=255)
                                                                                                                  tttCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTcc                                <  2:1093047/126‑1 (MQ=255)
                                                                                                                  tttCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTcc                                >  1:1093047/1‑126 (MQ=255)
                                                                                                                  tttATTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTcc                                <  2:1215354/126‑1 (MQ=255)
                                                                                                                    tCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGttt                >  1:1853395/1‑140 (MQ=255)
                                                                                                                    tCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGttt                >  1:873483/1‑140 (MQ=255)
                                                                                                                       ttttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTg             >  1:1659022/1‑140 (MQ=255)
                                                                                                                        tttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTga            <  1:414153/140‑1 (MQ=255)
                                                                                                                         ttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACCATTTCTCCGTGTTTgag           >  1:705962/1‑140 (MQ=255)
                                                                                                                         ttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTgag           >  1:1058416/1‑140 (MQ=255)
                                                                                                                             cTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGc       <  1:1283460/140‑1 (MQ=255)
                                                                                                                              tCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCa      >  2:588485/1‑140 (MQ=255)
                                                                                                                                cGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATg    >  1:297970/1‑140 (MQ=255)
                                                                                                                                 gACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGa   <  1:101567/140‑1 (MQ=255)
                                                                                                                                  acacCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGAc  >  2:1535897/1‑140 (MQ=255)
                                                                                                                                  acacCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGAc  >  1:1624857/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TCCGGAAACCGCTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGAC  >  NZ_CP034943/3246929‑3247198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: